LOCUS       Exported                2166 bp ds-DNA     circular SYN 07-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sunday, Nov 14, 2021 from SnapGene 4.3.11
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        27..290
                     /label=U6 promoter
                     /note="U6 promoter"
                     /note="color: #8080ff"
     misc_feature    292..323
                     /label=Type IC-CRISPR-repeat
                     /note="color: #a6acb3"
     misc_feature    324..348
                     /label=BbsI cassette
                     /note="SgRNA for SpCas9 with BbsI"
                     /note="color: #ff8000"
     misc_feature    349..380
                     /label=Type IC-CRISPR-repeat
                     /note="color: #a6acb3"
     misc_feature    578..1393
                     /label=aminoglycoside phosphotransferase (KanR)
                     /note="aminoglycoside phosphotransferase (KanR)"
                     /note="color: #ff8000"
ORIGIN
        1 ccaagctcgg atccgatatc gacgtcaagg tcgggcagga agagggccta tttcccatga
       61 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattaga attaatttga
      121 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
      181 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
      241 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gtcagccgcc
      301 tctaggcggc tgtgtgttga aacgggtctt cattattcca gaagacaatc agccgcctct
      361 aggcggctgt gtgttgaaac ttttttttct agatatcgct caatactgac catttaaatc
      421 atacctgacc tccatagcag aaagtcaaaa gcctccgacc ggaggctttt gacttgatcg
      481 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
      541 tgagttatcg agattttcag gagctaagga agctaaaatg agccatattc aacgggaaac
      601 gtcttgctcg aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg
      661 ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga
      721 tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga
      781 gatggtcagg ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat
      841 ccgtactcct gatgatgcat ggttactcac cactgcgatc ccagggaaaa cagcattcca
      901 ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct
      961 gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacggcgatc gcgtatttcg
     1021 tctcgctcag gcgcaatcac gaatgaataa cggtttggtt ggtgcgagtg attttgatga
     1081 cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt
     1141 ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga
     1201 ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga
     1261 tcttgccatc ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt
     1321 tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcact tgatgctcga
     1381 tgagtttttc taatgagggc ccaaatgtaa tcacctggct caccttcggg tgggcctttc
     1441 tgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgatg
     1501 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     1561 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     1621 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     1681 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     1741 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     1801 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     1861 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     1921 gctgaagcca gttacctcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     1981 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     2041 caagaagatc ctttgatttt ctaccgaaga aaggcccacc cgtgaaggtg agccagtgag
     2101 ttgattgcag tccagttacg ctggagtctg aggctcgtcc tgaatgatat caagcttgaa
     2161 ttcgtt
//
