LOCUS       pJH1969                 8690 bp    DNA        circular     01-NOV-2021
DEFINITION  
ACCESSION   
VERSION     
SOURCE      
  ORGANISM  
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     UTR             5336..6070
                     /locus_tag="unc-54 3UTR"
                     /label="unc-54 3UTR"
                     /ApEinfo_label="unc-54 3UTR"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     exon            5819..5973
                     /locus_tag="aex-5 exon1"
                     /label="aex-5 exon1"
                     /ApEinfo_label="aex-5 exon1"
                     /ApEinfo_fwdcolor="plum"
                     /ApEinfo_revcolor="plum"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          5285..5335
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     8545..8565
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     gene            3147..3147
                     /gene="nmr-1"
                     /db_xref="WormBase:WBGene00003774"
                     /locus_tag="nmr-1"
                     /label="nmr-1"
                     /ApEinfo_label="nmr-1"
                     /ApEinfo_fwdcolor="#817c7c"
                     /ApEinfo_revcolor="#817c7c"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      7495..8177
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3147..3147
                     /gene="nmr-1"
                     /standard_name="F07F6.6"
                     /note="contains similarity to Pfam domains PF00060
                     (Ligand-gated ion channel), PF01094 (Receptor family
                     ligand binding region); coded for by the following C.
                     elegans cDNAs: OSTF114A11_1, OSTF122C11_1, yk421g6.3,
                     yk421g6.5, AF318613"
                     /codon_start=1
                     /product="Nmda class glutamate receptor protein 1"
                     /protein_id="AAC46779.3"
                     /db_xref="GI:17976516"
                     /db_xref="WormBase:F07F6.6"
                     /db_xref="WormBase:WBGene00003774"
                     /translation="MFRISVIFIWIFLQLGYTIDYKVSVLIVSEPNQETFKELKVSVT
                     AAFVEVFGSTSYHLGNDTISAAFVDAKSGDNRLELTQDIVCSQMLNHSLASVIFSPLL
                     TSSSRFIDLVTSSAYTLSFYKLPVVGVMVRDAEFSKKNIYPTFVRPTAPLSDEAFVFL
                     HMLLSLKYRQVVVLSVKRDINADQFVEEFEKRRVEFKIIVQRYIEVELNENLNDTLAE
                     SFEEVTSNIIVLFAKKDDAVRIFANAGDLTGKGKVWIVSESAGEAHNVPNGSLGCRLG
                     QTAFSVLRDSFSILKSAMETIFRESKIDIFPPVECDRDSVDAEWNSLQAPALLNEICG
                     TSTSRVHFNDKCERIGVEYDIINFHMERKQVGNMVGDILRLDEDSIEWAGGTKPLEIS
                     LPKHLRVVTVADPPFVYTTPIGSPSQCAELGNTVVEWSIFDKIVVSGPWYSCPLTLEN
                     STEYFCCAGLAIDLLSNLSLPEANNSIDTSFTFSLHLNESYGVVQASETTGITISGVI
                     GELDGDTADMAIGGITINPERERIVDFTEPWLYHGIRILEKNIPRDSPMQSFLQPLQS
                     SLWTALFISVILVGLAIYCLDFKSPFERFYQADKEMEQDLKKEFELWIGKDADENVNF
                     GEAMWFVWGVLLNSGVSEKTPRSCSARVLGIVWCGFCMIMVASYTANLAAFLVLDQPE
                     KGLTGVTDPRLRNPSANFSFGTVLNSNVYQYFKRHVELSSMFRKMEPHNVRRASEAVH
                     SLLNGSLDAFIWDSTRLEFEAARHCELRTRGSLFGRSAYGIGLQKNSPWTPHITSAIL
                     RMSESGVMEKLDQKWIDRGGPNCVVEAHKSPARLGLVNMKDIFILVSSGVALGIFLSF
                     VEVSYGRRLADKGRRRRIVTRYFQKWHDLTLGKKRRPYRLKYNLDRMIVRRGFSGLER
                     CSFQELRERRQIRGLPTSKVDPYCFWPDLDFKDKPLVLFCSRCRNIVESDVHRETGLF
                     AFLSCFLFAILFLWPCSPLPCFLSSFSDFVHICPLCSHIMGRFRRARSTRFYV"
                     /locus_tag="Nmda class glutamate receptor protein 1"
                     /label="Nmda class glutamate receptor protein 1"
                     /ApEinfo_label="Nmda class glutamate receptor protein 1"
                     /ApEinfo_fwdcolor="#b9ff00"
                     /ApEinfo_revcolor="#b9ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    8517..8539
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="cornflower blue"
                     /ApEinfo_revcolor="cornflower blue"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6738..7397
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        2144..3146
                     /locus_tag="pnmr-1(short)"
                     /label="pnmr-1(short)"
                     /ApEinfo_label="pnmr-1(short)"
                     /ApEinfo_fwdcolor="#939dff"
                     /ApEinfo_revcolor="#939dff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     source          3240..5208
                     /organism="synthetic construct"
                     /mol_type="other DNA"
                     /db_xref="taxon:32630"
                     /locus_tag="source:synthetic construct"
                     /label="source:synthetic construct"
                     /ApEinfo_label="source:synthetic construct"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     intron          3174..3215
                     /locus_tag="intron(1)"
                     /label="intron(1)"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="#b0adad"
                     /ApEinfo_revcolor="#b0adad"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        27..3146
                     /locus_tag="pnmr-1long(noATG)"
                     /label="pnmr-1long(noATG)"
                     /ApEinfo_label="pnmr-1long(noATG)"
                     /ApEinfo_fwdcolor="#c8ffea"
                     /ApEinfo_revcolor="#c8ffea"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3933..4469
                     /note="Region: mutant calmodulin-peptide (D3)"
                     /locus_tag="Region: mutant calmodulin-peptide (D3)"
                     /label="Region: mutant calmodulin-peptide (D3)"
                     /ApEinfo_label="Region: mutant calmodulin-peptide (D3)"
                     /ApEinfo_fwdcolor="#ff0027"
                     /ApEinfo_revcolor="#ff0027"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3246..5207
                     /codon_start=1
                     /transl_table=11
                     /product="calcium sensor cameleon D3cpv"
                     /protein_id="ABF21066.1"
                     /db_xref="GI:94471597"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKN
                     GIKAHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAARMHDQLTEEQIAEFKEAFSLLDKDGDGTITTKELGTALRSLGQNPTEA
                     ELQDMINEVDADGNGTIYFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAE
                     LRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAKGGKRRWQKTGHAVR
                     AFGRLKKISSSGALELMDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNE
                     KRDHMVLLEFVTAAGITLGMDELYKGGSGGMVSKGEELFTGVVPILVELDGDVNGHKF
                     SVSGEGEGDATYGKLTLKLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFK
                     SAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEY
                     NYNSHNVYITADKQKNGIKANFKIRHNIE"
                     /locus_tag="calcium sensor cameleon D3cpv"
                     /label="calcium sensor cameleon D3cpv"
                     /ApEinfo_label="calcium sensor cameleon D3cpv"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4470..5204
                     /note="Region: circularly-permuted Venus fluorescent
                     protein"
                     /locus_tag="Region: circularly-permuted Venus fluorescent
                     prot"
                     /label="Region: circularly-permuted Venus fluorescent
                     prot"
                     /ApEinfo_label="Region: circularly-permuted Venus
                     fluorescent prot"
                     /ApEinfo_fwdcolor="#a0ff00"
                     /ApEinfo_revcolor="#a0ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3246..3932
                     /note="Region: truncated CFP"
                     /locus_tag="Region: truncated CFP"
                     /label="Region: truncated CFP"
                     /ApEinfo_label="Region: truncated CFP"
                     /ApEinfo_fwdcolor="#6100ff"
                     /ApEinfo_revcolor="#6100ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2909..2930
                     /locus_tag="OZM2875"
                     /label="OZM2875"
                     /ApEinfo_label="OZM2875"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(3373..3394)
                     /locus_tag="OZM2788"
                     /label="OZM2788"
                     /ApEinfo_label="OZM2788"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(3131..3147)
                     /locus_tag="OZM3526"
                     /label="OZM3526"
                     /ApEinfo_label="OZM3526"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(5480..5503)
                     /locus_tag="OZM1718"
                     /label="OZM1718"
                     /ApEinfo_label="OZM1718"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 AAGCTTGGGC TGCAgcatgc ggccgctgtg gtctcacttt gaacgacaaa agaaaaccaa
       61 atattgtaaa ggaatagtac aaataaaagg gaacaagtga taccagctga tggatgtagg
      121 tcagcgacaa aagacaggag acgtatagtt tgatgattat ggaaccaaac tcagaattta
      181 atgttggaga cgctataaaa attgaattga gaaattgaaa ccatattctt gaatatctct
      241 caatttggaa tttggaagta gccgtaaaat cggctagcta gaacataaaa ggactgattt
      301 gcaaccttga ccattcatga tatcatttca ataaaagaag agattccaat gaccaatctt
      361 caagaatcac aataatcaca acaaaactaa gcaaaagtaa caacaaattg ttcaggtaga
      421 caagtagaca tttggacaca ttttgcgggg atgtgatgac tgtataataa aaataagtgg
      481 acactgagag agagaggagg tcagcaggaa aaaagaacat cgaactgctt gacgaattga
      541 aattagaatg aggtgtcagg caggtgacga ttacatggtt aattattagt gttctttttc
      601 ttctttttgt ggatgccgat tagttgaaat aattgcatgg aaagtgagaa ttcaatgtga
      661 atctctttct tattatgttt caagtggtat ctggatggaa gcttgtactt ttatgaatcg
      721 aaaattgcgc ccaaggttga ttttgagatt attcagtaaa tttttgtgct gattattcag
      781 ttaatgtaga acagagacgc ggatgaaaac ttttttaatt tctaaaaaaa aagacaaaat
      841 gtggttaaaa agtttggaaa ataattgtta tatacttctt gagagcttat tcaaacggaa
      901 accaatcata aatagtcgaa tagtcattta gaaaagagaa actagttgat gtagagagat
      961 tcaaaaagta aaatatgcaa atgatccgtt tcttagattt agtaaacatt taccaaatga
     1021 aacaggaatt gtcgaaacaa tttagaaaag gagaaggaaa catttgttta ttttcccctt
     1081 taaatggcgg tagaggtaaa acagcaatac acatagtaca aggttatact actttcaaaa
     1141 gaatttgaca tcccgccgag aaaatgtggt gattatatgg taaagttttc agttggatat
     1201 tatcttctac agacggtgat tcaaaatgat caaaagggca agtgggtggt aagatttgaa
     1261 aaattgagat atagaacgtg cgattttggt aaaacttttt gaacacaagg catatttttg
     1321 aactttgaaa aacgcaaaat ttaaattttg ttaattacat atattttgaa cagattatga
     1381 atataagcaa ggctacataa aatatgtata ttttcttttt acaaatattc gtacactgtt
     1441 aaatttatag aaaaatcatt tatattcaga aaaacctatt tgaacaattt ttagttgctc
     1501 aataaccaat ttgcacaaat ttagaaaata taatgggggc ttttaaaaat ttaaatcctc
     1561 agcgaatatt cctttgcgat tttttcgaac gttttctttc agattaaaaa atctgaagca
     1621 gaagtaaatg aactaccata gaaaattcac caaggatcac atttttacaa agcctccgcc
     1681 caattgtaaa cttgggaatt tttgcttgtt ttcttttttt tttcaaaaaa ggaatagttg
     1741 ttttaaagta atttactccc ctatgctttc tccagcaatc agttgttctc cccatacttt
     1801 acacaattat caacaacttc acatagaaaa caaaacaaca aatcagaaat tcccaccggt
     1861 cacagtcaca gattccccat gttttgttca tctcatttct gcaagaaagt gacatcgctg
     1921 ccacccaatt cgacaaattg tccaagtggt catcaggaag atatgacctt ctttttaatg
     1981 ataatttaga ctacaaaggg cgtcaaaaaa gattcaatga tcaggggtaa ttgctgtaaa
     2041 atgtagtttg tatgtgtgtt taccgtagcc tataactgta aaaattggtt ttttaaaaag
     2101 tatttctgag attaaaaggc tccccaagga tgcacgattc tagctagaaa tgaacattcc
     2161 atgggttgtg aagatgcttt tatctcactt tagaatgtct gactccgccc ctctgtgtac
     2221 gaattgaagt gctcttttgt gatgatcata gctcctcctt gcggcgacgc ccgcacacag
     2281 cacgagatat tacacggaat acttatgtga ttcataggaa acaaggaaat cgaaaaaata
     2341 ataggatttt gaggaggtat ttttagatga atgcttatga tgttatgatg aacccaagaa
     2401 attctgaata gacatatgtc agaacggggt aggagctcca gtgtcgcttt tgaaataaaa
     2461 aatcaagtgg tatacttctc gtatataccc ttctgaacta ataaaataat ttatcttttg
     2521 aaaaagtttg aaaaagaaaa atgcccaaaa cttgaatttt aatgttttgt attttgagca
     2581 tatcgttcaa acgtcgtgat ctgaaagtag aaagatctaa atattcgtga cacagtttgg
     2641 tttgaaggtc ttagtggtaa ttttttatta cagaagcaac aaaaagttga catattttca
     2701 gaatttccaa aaaaacgttt aaaatttcaa tttcttcgat agagttctaa aaattttttg
     2761 gaacggattt tgtaatatcc gctgataacc gttcaagaac aatttgaatt ttgaaataat
     2821 ttaatcaact agtatccagt gctctttctc actgctccac ccatttttca acccatttcc
     2881 tttactcaat ttcgcccgtt ttccttttgc tcaaatgtgc ccaccaaatc atctttcttc
     2941 gtcttctttt ctcttattcg gaaggagaag aggtgaaagt agaatacgtt tttttgttga
     3001 aaagagaaga gcactgccac ttttctctta tttccatcct tctactacct cgttttactt
     3061 tccatttaaa aacccgttta aagttccaaa atttcaatta tcttgcttct gtttggaaca
     3121 cgacaaactt tagttttgtt acagatacCC GGGATTGGCC AAAGGACCCA AAGgtatgtt
     3181 tcgaatgata ctaacataac atagaacatt ttcagGAGGA CCCTTGGCTA GCGTCGACGg
     3241 atcccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     3301 tggacggcga cgtaaacggc cacaggttca gcgtgtccgg cgagggcgag ggcgatgcca
     3361 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     3421 ccaccctcgt gaccaccctg acctggggcg tgcagtgctt cagccgctac cccgaccaca
     3481 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgtacca
     3541 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     3601 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     3661 ggcacaagct ggagtacaac tacatcagcc acaacgtcta tatcaccgcc gacaagcaga
     3721 agaacggcat caaggcccac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     3781 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     3841 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     3901 tggtcctgct ggagttcgtg accgccgccc gcatgcatga ccaactgaca gaagagcaga
     3961 ttgcagagtt caaagaagcc ttctcattat tggacaagga tggggacggc accatcacca
     4021 caaaggaact tggcaccgct ctgaggtcgc ttggacaaaa cccaacggaa gcagaattgc
     4081 aggatatgat caatgaagtc gatgctgatg gcaatggaac gatttacttt cctgaatttc
     4141 ttactatgat ggctagaaaa atgaaggaca cagacagcga agaggaaatc cgagaagcat
     4201 tccgtgtttt tgacaaggat gggaacggct acatcagcgc tgctgaatta cgtcacgtca
     4261 tgacaaacct cggggagaag ttaacagatg aagaagttga tgaaatgata agggaagcag
     4321 atatcgatgg tgatggccaa gtaaactatg aagagtttgt acaaatgatg acagcaaagg
     4381 gggggaagag gcgctggcag aaaacaggcc atgctgtccg agcattcgga agactgaaga
     4441 agatctccag ctccggggca ctggagctca tggacggcgg cgtgcagctc gccgaccact
     4501 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4561 gctaccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4621 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggtggcagcg
     4681 gtggcatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     4741 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     4801 cctacggcaa gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc
     4861 ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca
     4921 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     4981 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     5041 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     5101 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga
     5161 agaacggcat caaggccaac ttcaagatcc gccacaacat cgagtaagAA TTCCAACTGA
     5221 GCGCCGGTCG CTACCATTAC CAACTTGTCT GGTGTCAAAA ATAATAGGGG CCGCTGTCAT
     5281 CAGAgtaagt ttaaactgag ttctactaac taacgagtaa tatttaaatt ttcagCATCT
     5341 CGCGCCCGTG CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC
     5401 TCCCTGTGCT CCCACCCCCT ATTTTTGTTA TTATCAAAAA AACTTCTTCT TAATTTCTTT
     5461 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA
     5521 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT
     5581 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTTTAC TTCTGATAAA
     5641 TTTTTTTTGA AACATCATAG AAAAAACCGC ACACAAAATA CCTTATCATA TGTTACGTTT
     5701 CAGTTTATGA CCGCAATTTT TATTTCTTCG CACGTCTGGG CCTCTCATGA CGTCAAATCA
     5761 TGCTCATCGT GAAAAAGTTT TGGAGTATTT TTGGAATTTT TCAATCAAGT GAAAGTTTAT
     5821 GAAATTAATT TTCCTGCTTT TGCTTTTTGG GGGTTTCCCC TATTGTTTGT CAAGAGTTTC
     5881 GAGGACGGCG TTTTTCTTGC TAAAATCACA AGTATTGATG AGCACGATGC AAGAAAGATC
     5941 GGAAGAAGGT TTGGGTTTGA GGCTCAGTGG AAGGTGAGTA GAAGTTGATA ATTTGAAAGT
     6001 GGAGTAGTGT CTATGGGGTT TTTGCCTTAA ATGACAGAAT ACATTCCCAA TATACCAAAC
     6061 ATAACTGTTT CCTACTAGTC GGCCGTACGG GCCCTTTCGT CTCGCGCGTT TCGGTGATGA
     6121 CGGTGAAAAC CTCTGACACA TGCAGCTCCC GGAGACGGTC ACAGCTTGTC TGTAAGCGGA
     6181 TGCCGGGAGC AGACAAGCCC GTCAGGGCGC GTCAGCGGGT GTTGGCGGGT GTCGGGGCTG
     6241 GCTTAACTAT GCGGCATCAG AGCAGATTGT ACTGAGAGTG CACCATATGC GGTGTGAAAT
     6301 ACCGCACAGA TGCGTAAGGA GAAAATACCG CATCAGGCGG CCTTAAGGGC CTCGTGATAC
     6361 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT
     6421 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT
     6481 ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA
     6541 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG
     6601 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC
     6661 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG
     6721 AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC
     6781 GTATTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG
     6841 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT
     6901 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG
     6961 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG
     7021 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC
     7081 CTGTAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT
     7141 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT
     7201 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC
     7261 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA
     7321 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT
     7381 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT
     7441 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA
     7501 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA
     7561 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC
     7621 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG
     7681 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG
     7741 GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC
     7801 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT
     7861 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG
     7921 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCATTGAGAA AGCGCCACGC
     7981 TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC
     8041 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC
     8101 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA
     8161 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT
     8221 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG
     8281 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG
     8341 AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC
     8401 ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC
     8461 TCACTCATTA GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA
     8521 TTGTGAGCGG ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTgt
     8581 aagtttaaac atgatcttac taactaacta ttctcattta aattttcagA GCTTAAAAAT
     8641 GGCTGAAATC ACTCACAACG ATGGATACGC TAACAACTTG GAAATGAAAT 
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