LOCUS       Exported                8355 bp ds-DNA     circular SYN 08-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 9, 2021 from SnapGene Viewer 5.0.7
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    33..136
                     /label=AAV ITR
     enhancer        278..657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        659..936
                     /label=chicken beta-actin promoter
     promoter        1526..1544
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1577..3346
                     /label=mGrn
     CDS             3365..3394
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=c-myc tag
                     /translation="EQKLISEEDL"
     misc_feature    3440..4028
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3911..3922)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    4039..4552
                     /label=Rabbit B Globulin Poly A Terminator
     polyA_signal    4184..4239
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_feature    4637..4740
                     /label=AAV ITR
     misc_feature    4769..4837
                     /label=LacZ alpha
     rep_origin      5138..5651
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        6398..6502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6503..7363
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7534..8122
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggccaactcc
      121 atcactaggg gttccttgta gttaatgatt aacccgccat gctacttatc tacgtagcca
      181 tgctctagcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat gagcttggcc
      241 cattgcatac gttgtatcca tatcataata tgtactcgac attgattatt gactagttat
      301 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
      361 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
      421 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
      481 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
      601 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      661 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
      721 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
      781 cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg
      841 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
      901 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgtt gccttcgccc
      961 cgtgccccgc tccgcgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc
     1021 ccacaggtga gcgggcggga cggcccttct cctccgggct gtaattagcg cttggtttaa
     1081 tgacggctcg tttcttttct gtggctgcgt gaaagcctta aagggctccg ggagggccct
     1141 ttgtgcgggg gggagcggct cggggggtgc gtgcgtgtgt gtgtgcgtgg ggagcgccgc
     1201 gtgcggcccg cgctgcccgg cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg
     1261 ctccgcgtgt gcgcgagggg agcgcggccg ggggcggtgc cccgcggggg acggctgcct
     1321 tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg gctctagagc
     1381 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     1441 tgttgtgctg tctcatcatt ttggcaaaga attctcgagc tctctggcta actagagaac
     1501 ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc caagctggct
     1561 agttaagctt gccgccacca tgtgggtcct gatgagctgg ctggccttcg cggcagggct
     1621 ggtagccgga acacagtgtc cagatgggca gttctgccct gttgcctgct gccttgacca
     1681 gggaggagcc aactacagct gctgtaaccc tcttctggac acatggccta gaataacgag
     1741 ccatcatcta gatggctcct gccagaccca tggccactgt cctgctggct attcttgtct
     1801 tctcactgtg tctgggactt ccagctgctg cccgttctct aagggtgtgt cttgtggtga
     1861 tggctaccac tgctgccccc agggcttcca ctgtagtgca gatgggaaat cctgcttcca
     1921 gatgtcagat aaccccttgg gtgctgtcca gtgtcctggg agccagtttg aatgtcctga
     1981 ctctgccacc tgctgcatta tggttgatgg ttcgtgggga tgttgtccca tgccccaggc
     2041 ctcttgctgt gaagacagag tgcattgctg tccccatggg gcctcctgtg acctggttca
     2101 cacacgatgc gtttcaccca cgggcaccca caccctacta aagaagttcc ctgcacaaaa
     2161 gaccaacagg gcagtgtctt tgcctttttc tgtcgtgtgc cctgatgcta agacccagtg
     2221 tcccgatgat tctacctgct gtgagctacc cactgggaag tatggctgct gtccaatgcc
     2281 caatgccatc tgctgttccg accacctgca ctgctgcccc caggacactg tatgtgacct
     2341 gatccagagt aagtgcctat ccaagaacta caccacggat ctcctgacca agctgcctgg
     2401 atacccagtg aaggaggtga agtgcgacat ggaggtgagc tgccctgaag gatatacctg
     2461 ctgccgcctc aacactgggg cctggggctg ctgtccattt gccaaggccg tgtgttgtga
     2521 ggatcacatt cattgctgcc cggcagggtt tcagtgtcac acagagaaag gaacctgcga
     2581 aatgggtatc ctccaagtac cctggatgaa gaaggtcata gcccccctcc gcctgccaga
     2641 cccacagatc ttgaagagtg atacaccttg tgatgacttc actaggtgtc ctacaaacaa
     2701 tacctgctgc aaactcaatt ctggggactg gggctgctgt cccatcccag aggctgtctg
     2761 ctgctcagac aaccagcatt gctgccctca gggcttcaca tgtctggctc aggggtactg
     2821 tcagaaggga gacacaatgg tggctggcct ggagaagata cctgcccgcc agacaacccc
     2881 gctccaaatt ggagatatcg gttgtgacca gcataccagc tgcccagtag ggcaaacctg
     2941 ctgcccaagc ctcaagggaa gttgggcctg ctgccagctg ccccatgctg tgtgctgtga
     3001 ggaccggcag cactgttgcc cggccgggta cacctgcaat gtgaaggcga ggacctgtga
     3061 gaaggatgtc gattttatcc agcctcccgt gctcctgacc ctcggcccta aggttgggaa
     3121 tgtggagtgt ggagaagggc atttctgcca tgataaccag acctgttgta aagacagtgc
     3181 aggagtctgg gcctgctgtc cctacctaaa gggtgtctgc tgtagagatg gacgtcactg
     3241 ttgccccggt ggcttccact gttcagccag gggaaccaag tgtttgcgaa agaagattcc
     3301 tcgctgggac atgtttttga gggatccggt cccaagaccg ctactggctc gaggtcaccc
     3361 attcgaacaa aaactcatct cagaagagga tctgaatatg cattaagcgg ccgctagcga
     3421 tatcggtacc ggatccgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3481 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3541 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3601 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3661 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3721 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     3781 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     3841 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     3901 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     3961 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4021 ctccccgcct ggatccaatt cactcctcag gtgcaggctg cctatcagaa ggtggtggct
     4081 ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc tgccaaaaat
     4141 tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt
     4201 ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg
     4261 gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgccata
     4321 tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga aacagccccc
     4381 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     4441 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     4501 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatgaag
     4561 atccctcgac ctgcagccca agctctagag catggctacg tagataagta gcatggcggg
     4621 ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4681 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4741 gcctcagtga gcgagcgagc gcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4801 cccttcccaa cagttgcgca gcctgaatgg cgaatggcga ttctgttgca atggctggcg
     4861 gtaatattgt tctggatatt accagcaagg ccgatagttt gagttcttct actcaggcaa
     4921 gtgatgttat tactaatcaa agaagtattg cgacaacggt taatttgcgt gatggacaga
     4981 ctcttttact cggtggcctc actgattata aaaacacttc tcaggattct ggcgtaccgt
     5041 tcctgtctaa aatcccttta atcggcctcc tgtttagctc ccgctctgat tctaacgagg
     5101 aaagcacgtt atacgtgctc gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta
     5161 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     5221 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     5281 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     5341 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     5401 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     5461 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     5521 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     5581 acgtttacaa tttaaatatt tgcttataca atcttcctgt ttttggggct tttctgatta
     5641 tcaaccgggg tacatatgat tgacatgcta gttttacgat taccgttcat cgattctctt
     5701 gtttgctcca gactctcagg caatgacctg atagcctttg tagagacctc tcaaaaatag
     5761 ctaccctctc cggcatgaat ttatcagcta gaacggttga atatcatatt gatggtgatt
     5821 tgactgtctc cggcctttct cacccgtttg aatctttacc tacacattac tcaggcattg
     5881 catttaaaat atatgagggt tctaaaaatt tttatccttg cgttgaaata aaggcttctc
     5941 ccgcaaaagt attacagggt cataatgttt ttggtacaac cgatttagct ttatgctctg
     6001 aggctttatt gcttaatttt gctaattctt tgccttgcct gtatgattta ttggatgttg
     6061 gaatgccctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg
     6121 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc
     6181 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     6241 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     6301 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     6361 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     6841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     6901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     6961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7561 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7621 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7681 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7741 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7801 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7861 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7921 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7981 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8041 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8101 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8161 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8221 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8281 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8341 gccgattcat taatg
//
