LOCUS       Exported               13823 bp DNA     circular SYN 10-DEC-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Dec 10, 2021 from SnapGene 5.3.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13823
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     protein_bind    14..32
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    49..67
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    85..103
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    121..139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    156..174
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    192..210
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             316..1002
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from 
                     rat"
                     /label=APOBEC-1
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFL
                     SRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSN
                     EAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHI
                     LWATGLK"
     misc_feature    1048..1557
                     /label=YTHdomain
     CDS             1564..1590
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1591..1644
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1645..2361
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     promoter        2372..2583
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2605..3201
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             3211..3267
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3268..4014
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4035..4623
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4506..4517)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4695..4928
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4960..5184
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5230..5658
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5672..6001
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5852..5987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6049..6096
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6115..6489
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6619..6740
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6789..6805)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6837..6867)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     rep_origin      complement(7191..7779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7950..8810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8811..8915)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        9181..9560
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9562..9760
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9778..9958
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10005..10130
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10623..10856
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11383..11500
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        11551..11791
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        13681..13756
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        join(13793..13823,1..284)
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    13801..13819
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc cctatcagtg
       61 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
      121 tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata gagaacgtat
      181 gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt gtacggtggg
      241 aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga ataccggttc
      301 tagagcgctg ccaccatgag ctcagagact ggcccagtgg ctgtggaccc cacattgaga
      361 cggcggatcg agccccatga gtttgaggta ttcttcgatc cgagagagct ccgcaaggag
      421 acctgcctgc tttacgaaat taattggggg ggccggcact ccatttggcg acatacatca
      481 cagaacacta acaagcacgt cgaagtcaac ttcatcgaga agttcacgac agaaagatat
      541 ttctgtccga acacaaggtg cagcattacc tggtttctca gctggagccc atgcggcgaa
      601 tgtagtaggg ccatcactga attcctgtca aggtatcccc acgtcactct gtttatttac
      661 atcgcaaggc tgtaccacca cgctgacccc cgcaatcgac aaggcctgcg ggatttgatc
      721 tcttcaggtg tgactatcca aattatgact gagcaggagt caggatactg ctggagaaac
      781 tttgtgaatt atagcccgag taatgaagcc cactggccta ggtatcccca tctgtgggta
      841 cgactgtacg ttcttgaact gtactgcatc atactgggcc tgcctccttg tctcaacatt
      901 ctgagaagga agcagccaca gctgacattc tttaccatcg ctcttcagtc ttgtcattac
      961 cagcgactgc ccccacacat tctctgggcc accgggttga aaagcggcag cgagactccc
     1021 gggacctcag agtccgccac accagaaggc cgggttttca tcattaagag ctactctgag
     1081 gacgatattc accgttccat taagtataat atttggtgca gcacagagca tggtaacaag
     1141 agactggatg ctgcttatcg ttccatgaac gggaaaggcc ccgtttactt acttttcagt
     1201 gtcaacggca gtggacactt ctgtggcgtg gcagaaatga aatctgctgt ggactacaac
     1261 acatgtgcag gtgtgtggtc ccaggacaaa tggaagggtc gttttgatgt caggtggatt
     1321 tttgtgaagg acgttcccaa tagccaactg cgacacattc gcctagagaa caacgagaat
     1381 aaaccagtga ccaactctag ggacactcag gaagtgcctc tggaaaaggc taagcaggtg
     1441 ttgaaaatta tagccagcta caagcacacc acttccattt ttgatgactt ctcacactat
     1501 gagaaacgcc aagaggaaga agaaagtgtt aaaaaggaac gtcaaggtcg tgggaaactc
     1561 gagtacccct acgacgtgcc cgactacgcc gagggcagag gaagtctgct aacatgcggt
     1621 gacgtcgagg agaatcctgg cccagtgagc aagggcgagg agctgttcac cggggtggtg
     1681 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1741 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     1801 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     1861 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     1921 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     1981 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2041 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2101 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2161 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2221 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     2281 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2341 atggacgagc tgtacaagta agctagcatc cgggcagagc gcacatcgcc cacagtcccc
     2401 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
     2461 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     2521 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     2581 cagactgcga tcgcaatgta cagtatgacc gagtacaagc ccacggtgcg cctcgccacc
     2641 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     2701 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     2761 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     2821 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     2881 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     2941 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg agtctcgccc
     3001 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     3061 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     3121 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     3181 atgacccgca agcccggtgc cggttccggc gcaacaaact tctctctgct gaaacaagcc
     3241 ggagatgtcg aagagaatcc tggaccgatg tctagactgg acaagagcaa agtcataaac
     3301 ggagctctgg aattactcaa tggtgtcggt atcgaaggcc tgacgacaag gaaactcgct
     3361 caaaagctgg gagttgagca gcctaccctg tactggcacg tgaagaacaa gcgggccctg
     3421 ctcgatgccc tgccaatcga gatgctggac aggcatcata cccacttctg ccccctggaa
     3481 ggcgagtcat ggcaagactt tctgcggaac aacgccaagt cataccgctg tgctctcctc
     3541 tcacatcgcg acggggctaa agtgcatctc ggcacccgcc caacagagaa acagtacgaa
     3601 accctggaaa atcagctcgc gttcctgtgt cagcaaggct tctccctgga gaacgcactg
     3661 tacgctctgt ccgccgtggg ccactttaca ctgggctgcg tattggagga acaggagcat
     3721 caagtagcaa aagaggaaag agagacacct accaccgatt ctatgccccc acttctgaga
     3781 caagcaattg agctgttcga ccggcaggga gccgaacctg ccttcctttt cggcctggaa
     3841 ctaatcatat gtggcctgga gaaacagcta aagtgcgaaa gcggcgggcc gaccgacgcc
     3901 cttgacgatt ttgacttaga catgctccca gccgatgccc ttgacgactt tgaccttgat
     3961 atgctgcctg ctgacgctct tgacgatttt gaccttgaca tgctccccgg gtaactcgag
     4021 cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     4081 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     4141 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     4201 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     4261 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     4321 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     4381 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct
     4441 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     4501 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     4561 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     4621 cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     4681 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     4741 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     4801 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     4861 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     4921 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     4981 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     5041 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     5101 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     5161 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     5221 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5281 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5341 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5401 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5461 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5521 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5581 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5641 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5701 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     5761 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     5821 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5881 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5941 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6001 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     6061 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     6121 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     6181 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     6241 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     6301 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     6361 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     6421 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     6481 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     6541 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     6601 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6661 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6721 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     6781 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6901 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6961 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7921 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8581 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8641 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8701 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8761 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8941 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     9001 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     9061 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     9121 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     9181 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     9241 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     9301 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     9361 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     9421 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     9481 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     9541 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     9601 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     9661 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     9721 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     9781 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9841 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9901 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9961 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
    10021 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
    10081 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
    10141 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
    10201 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
    10261 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
    10321 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
    10381 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
    10441 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
    10501 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
    10561 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10621 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10681 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10741 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    10801 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10861 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10921 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    10981 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    11041 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    11101 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    11161 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
    11221 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
    11281 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
    11341 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
    11401 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    11461 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    11521 cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat tccttcatat
    11581 ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac tgtaaacaca
    11641 aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta gtttgcagtt
    11701 ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa gtatttcgat
    11761 ttcttggctt tatatatctt gtggaaagga cgaaacaccg gagacggttg taaatgagca
    11821 cacaaaatac acatgctaaa atattatatt ctatgacctt tataaaatca accaaaatct
    11881 tctttttaat aactttagta tcaataatta gaatttttat gttccttttt gcaaactttt
    11941 aataaaaatg agcaaaataa aaaaacgcta gttttagtaa ctcgcgttgt tttcttcacc
    12001 tttaataata gctactccac cacttgttcc taagcggtca gctcctgctt caatcatttt
    12061 ttgagcatct tcaaatgttc taactccacc agctgcttta actaaagcat tgtctttaac
    12121 aactgacttc attagtttaa catcttcaaa tgttgcacct gattttgaaa atcctgttga
    12181 tgttttaaca aattctaatc cagcttcaac agctatttca caagctttca tgatttcttc
    12241 ttttgttaat aaacaatttt ccataataca tttaacaaca tgtgatccag ctgctttttt
    12301 tacagctttc atgtcttcta aaactaattc ataatttttg tcttttaatg caccaatatt
    12361 taataccata tcaatttctg ttgcaccatc tttaattgct tcagaaactt cgaatgcttt
    12421 tgtagctgtt gtgcatgcac ctagaggaaa acctacaaca tttgttattc ctacatttgt
    12481 gccttttaat aattctttac aatagcttgt tcaatatgaa ttaacacaaa ctgttgcaaa
    12541 atcaaattca attgcttcat cacataattg tttaatttca gctttcgtag catcttgttt
    12601 taataatgtg tgatctatat atttgtttag tttcattttt tctcctatat attcattttt
    12661 aattttaatt ctttaataat ttcgtctact ttaactttag cgttttgaac agattcacca
    12721 acacctataa aataaatttt tagtttaggt tcagttccac ttgggcgaac agcaaatcat
    12781 gacttatctt ctaaataaaa ttttagtaag tcttgtcctg gcatattata cattccatcg
    12841 atgtagtctt caacattaac aactttaagt ccagcaattt gagttaaggg tgttgctctc
    12901 aatgatttca ttaatggttc aatttttaat ttcttttctt ctggtttaaa attcaagttt
    12961 aaagtgaaag tgtaatatgc acccatttct ttaaataaat cttctaaata gtctactaat
    13021 gttttatttt gttttttata aaatcaagca gcctctgcta ttaatataga agcttgtatt
    13081 ccatctttat ctctagctga gtcatcaatt acatatccat aactttcttc ataagcaaaa
    13141 acaaaattta atccgttatc ttcttcttta gcaatttctc tacccattca tttaaatcca
    13201 gttaaagttt ttacaatatt aactccatat ttttcatgag cgattctatc acccaaatca
    13261 cttgttacaa aacttgaata tagagccgga ttttttggaa tgctatttaa gcgttttaga
    13321 tttgataatt ttcaatcaat taaaattggt cctgtttgat ttccatctaa tcttacaaaa
    13381 tgaccatcat gttttattgc cattccaaat ctgtcagcat ctgggtcatt cataataata
    13441 atatctgcat catgtttaat accatattca agcggtattt ttcatgcagg atcaaattct
    13501 ggatttggat ttacaacatt tttaaatgtt tcatcttcaa atgcatgctc ttcaacctca
    13561 ataacgttat atcctgattc acgtaatatt tttggggtaa atttagttcc tgttccatta
    13621 actgcgctaa aaataatttt taaatctttt ttagcttctt gctctttttt gtacgtctct
    13681 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    13741 ggcaccgagt cggtgctttt ttgaattcac tttggccgcg aatcgatatg tcgagtttac
    13801 tccctatcag tgatagagaa cgt
//
