LOCUS       pSYC_190                5622 bp    DNA        circular     10-NOV-2021
DEFINITION  .
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     primer_bind     complement(1916..1935)
                     /vntifkey="28"
                     /locus_tag="T3"
                     /label="T3"
                     /ApEinfo_label="T3"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(1953..1973)
                     /vntifkey="28"
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     35..52
                     /vntifkey="28"
                     /locus_tag="SP6"
                     /label="SP6"
                     /ApEinfo_label="SP6"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(2341..3023)
                     /vntifkey="33"
                     /locus_tag="ColE1\origin"
                     /label="ColE1\origin"
                     /ApEinfo_label="ColE1\origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(4241..4547)
                     /vntifkey="33"
                     /locus_tag="F1\ori"
                     /label="F1\ori"
                     /ApEinfo_label="F1\ori"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(4566..4634)
                     /vntifkey="4"
                     /locus_tag="LacZ\alpha"
                     /label="LacZ\alpha"
                     /ApEinfo_label="LacZ\alpha"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_binding    complement(1979..2001)
                     /vntifkey="20"
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(3121..3780)
                     /vntifkey="4"
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     polyA_signal    1675..1866
                     /vntifkey="25"
                     /locus_tag="SV40\late\polyA"
                     /label="SV40\late\polyA"
                     /ApEinfo_label="SV40\late\polyA"
                     /ApEinfo_fwdcolor="pink"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1724..1774)
                     /vntifkey="21"
                     /locus_tag="SV40\pA"
                     /label="SV40\pA"
                     /ApEinfo_label="SV40\pA"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1414..1491
                     /vntifkey="21"
                     /locus_tag="3xFLAG"
                     /label="3xFLAG"
                     /ApEinfo_label="3xFLAG"
                     /ApEinfo_fwdcolor="#00ffff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1537..1602
                     /vntifkey="21"
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    85..1380
                     /locus_tag="hGFAP"
                     /label="hGFAP"
                     /ApEinfo_label="hGFAP"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     modified_base   319..319
                     /locus_tag="C>T (R79C)"
                     /label="C>T (R79C)"
                     /ApEinfo_label="C>T (R79C)"
                     /ApEinfo_fwdcolor="#92ff65"
                     /ApEinfo_revcolor="#92ff65"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        4638..5622
                     /locus_tag="CMV IE94 Promoter"
                     /label="CMV IE94 Promoter"
                     /ApEinfo_label="CMV IE94 Promoter"
                     /ApEinfo_fwdcolor="#346ee0"
                     /ApEinfo_revcolor="#346ee0"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcaGG ATCCatggag aggagacgca tcacctccgc tgctcgccgc
      121 tcctacgtct cctcagggga gatgatggtg gggggcctgg ctcctggccg ccgtctgggt
      181 cctggcaccc gcctctccct ggctcgaatg ccccctccac tcccgacccg ggtggatttc
      241 tccctggctg gggcactcaa tgctggcttc aaggagaccc gggccagtga gcgggcagag
      301 atgatggagc tcaatgactg ctttgccagc tacatcgaga aggttcgctt cctggaacag
      361 caaaacaagg cgctggctgc tgagctgaac cagctgcggg ccaaggagcc caccaagctg
      421 gcagacgtct accaggctga gctgcgagag ctgcggctgc ggctcgatca actcaccgcc
      481 aacagcgccc ggctggaggt tgagagggac aatctggcac aggacctggc cactgtgagg
      541 cagaagctcc aggatgaaac caacctgagg ctggaagccg agaacaacct ggctgcctat
      601 agacaggaag cagatgaagc caccctggcc cgtctggatc tggagaggaa gattgagtcg
      661 ctggaggagg agatccggtt cttgaggaag atccacgagg aggaggttcg ggaactccag
      721 gagcagctgg cccgacagca ggtccatgtg gagcttgacg tggccaagcc agacctcacc
      781 gcagccctga aagagatccg cacgcagtat gaggcaatgg cgtccagcaa catgcatgaa
      841 gccgaagagt ggtaccgctc caagtttgca gacctgacag acgctgctgc ccgcaacgcg
      901 gagctgctcc gccaggccaa gcacgaagcc aacgactacc ggcgccagtt gcagtccttg
      961 acctgcgacc tggagtctct gcgcggcacg aacgagtccc tggagaggca gatgcgcgag
     1021 caggaggagc ggcacgtgcg ggaggcggcc agttatcagg aggcgctggc gcggctggag
     1081 gaagaggggc agagcctcaa ggacgagatg gcccgccact tgcaggagta ccaggacctg
     1141 ctcaatgtca agctggccct ggacatcgag atcgccacct acaggaagct gctagagggc
     1201 gaggagaacc ggatcaccat tcccgtgcag accttctcca acctgcagat tcgagaaacc
     1261 agcctggaca ccaagtctgt gtcagaaggc cacctcaaga ggaacatcgt ggtgaagacc
     1321 gtggagatgc gggatggaga ggtcattaag gagtccaagc aggagcacaa ggatgtgatg
     1381 ATGATATCtg gcgcgcctac catggggccc ggcgactaca aggatgacga tgacaagggc
     1441 gactacaagg atgacgatga caagggcgac tacaaggatg acgatgacaa gggccggcct
     1501 aagatcaccg gtcccgggct taaggagctc gcatgcggaa gcggagctac taacttcagc
     1561 ctgctgaagc aggctggaga cgtggaggag aaccctggac ctagatctgg aattcaaggc
     1621 ctctcgagcc tctagatagc tagcctccct atagtgagtc gtattacgta gatccagaca
     1681 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1741 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     1801 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1861 ttttttaatt cgcggccgcg gcgccaatgc attgggcccg gtacccagct tttgttccct
     1921 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1981 ttgttatccg ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg
     2041 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     2101 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     2161 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2221 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2281 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2341 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2401 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2461 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2521 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2581 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2641 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2701 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2761 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2821 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2881 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2941 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     3001 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     3061 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     3121 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3181 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3241 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3301 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3361 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3421 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3481 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3541 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3601 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3661 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3721 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3781 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3841 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3901 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3961 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     4021 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     4081 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     4141 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4201 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4261 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4321 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4381 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4441 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4501 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4561 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4621 tcgctattac gccagtcgac catagccaat tcaatatggc gtatatggac tcatgccaat
     4681 tcaatatggt ggatctggac ctgtgccaat tcaatatggc gtatatggac tcgtgccaat
     4741 tcaatatggt ggatctggac cccagccaat tcaatatggc ggacttggca ccatgccaat
     4801 tcaatatggc ggacttggca ctgtgccaac tggggagggg tctacttggc acggtgccaa
     4861 gtttgaggag gggtcttggc cctgtgccaa gtccgccata ttgaattggc atggtgccaa
     4921 taatggcggc catattggct atatgccagg atcaatatat aggcaatatc caatatggcc
     4981 ctatgccaat atggctattg gccaggttca atactatgta ttggccctat gccatatagt
     5041 attccatata tgggttttcc tattgacgta gatagcccct cccaatgggc ggtcccatat
     5101 accatatatg gggcttccta ataccgccca tagccactcc cccattgacg tcaatggtct
     5161 ctatatatgg tctttcctat tgacgtcata tgggcggtcc tattgacgta tatggcgcct
     5221 cccccattga cgtcaattac ggtaaatggc ccgcctggct caatgcccat tgacgtcaat
     5281 aggaccaccc accattgacg tcaatgggat ggctcattgc ccattcatat ccgttctcac
     5341 gccccctatt gacgtcaatg acggtaaatg gcccacttgg cagtacatca atatctatta
     5401 atagtaactt ggcaagtaca ttactattgg aagtacgcca gggtacattg gcagtactcc
     5461 cattgacgtc aatggcggta aatggcccgc gatggctgcc aagtacatcc ccattgacgt
     5521 caatggggag gggcaatgac gcaaatgggc gttccattga cgtaaatggg cggtaggcgt
     5581 gcctaatggg aggtctatat aagcaatgct cgtttaggga ac 
//
