LOCUS       pBBK26r_pHomL-TEF2p-Neo 6682 bp ds-DNA     circular     18-MAY-2022
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          510..527
                     /label="OR0070. pYTK083 chk2 F"
                     /note="sequence: ttactgtcatgccatccg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(633..652)
                     /label="BB1006. AmpR seq1 R"
                     /note="sequence: taaagttctgctatgtggcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          741..759
                     /label="BB1478. AmpR F"
                     /note="sequence: gtgcacccaactgatcttc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(1869..1888)
                     /label="BB1476. HomArmL seq R"
                     /note="sequence: ggcatgagttatggttgcac"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(2724..2743)
                     /label="BB1477. ConLS R"
                     /note="sequence: gggttaatgtctgtgatcgc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3525
                     /label="TEF2p (2)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3337..3357
                     /label="BB868. TEF2p F"
                     /note="sequence: agtgctatttctggcttttcc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3526..3526
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3527..4234
                     /label="ymNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3527..4234
                     /label="Translation 3527-4234"
     primer          complement(3616..3633)
                     /label="BB1179. mNeon R"
                     /note="sequence: ccttgaccgaccatatcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3803..3823
                     /label="BB899. NeonGreen seq F"
                     /note="sequence: ggttaccaagtccacagaacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4235..4258
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4235..4258
                     /label="Translation 4235-4258"
     misc_feature    4258..4261
                     /label="3a>3b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4262..5287
                     /label="GFP dropout cassette"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             4417..4428
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             4435..5148
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      5160..5231
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      5247..5274
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5288..5291
                     /label="3>4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5292..5525
                     /label="SSA1t (4)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5479..5501)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5526..5529
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5530..5698
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5699..5702
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5703..5822
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5703..6576
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5801..5821)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5827..5940
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5945..6080
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6052..6072
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6085..6204
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6209..6328
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6306..6325)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6333..6452
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6457..6576
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6577..6580
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gttgataggt caagatcaat gtaaacaatt actttgttat gtagagtttt tttagctacc
     2881 tatattccac cataacatca atcatgcggt tgctggtgta tttaccaata atgtttaatg
     2941 tatatatata tatatatata tggggccgta tacttacata tagtagatgt caagcgtagg
     3001 cgcttcccct gccggctgtg agggcgccat aaccaaggta tctatagacc gccaatcagc
     3061 aaactacctc cgtacattca tgttgcaccc acacatttat acacccagac cgcgacaaat
     3121 tacccataag gttgtttgtg acggcgtcgt acaagagaac gtgggaactt tttaggctca
     3181 ccaaaaaaga aagaaaaaat acgagttgct gacagaagcc tcaagaaaaa aaaaattctt
     3241 cttcgactat gctggaggca gagatgatcg agccggtagt taactatata tagctaaatt
     3301 ggttccatca ccttcttttc tggtgtcgct ccttctagtg ctatttctgg cttttcctat
     3361 tttttttttc catttttctt tctctctttc taatatataa attctcttgc attttctatt
     3421 tttctctcta tctattctac ttgtttattc ccttcaaggt ttttttttaa ggagtacttg
     3481 tttttagaat atacggtcaa cgaactataa ttaactaaac agatcTatgg tttctaaggg
     3541 tgaagaagat aacatggctt ctttgccagc cacccacgag ttgcacatct tcggttccat
     3601 taacggtgtc gacttcgata tggtcggtca aggtaccggt aacccaaacg acggttacga
     3661 agaattgaac ttgaagtcta ccaagggcga cttgcagttc tccccttgga ttttggttcc
     3721 acacatcggt tacggtttcc accaatactt gccataccca gacggtatgt ccccattcca
     3781 agccgccatg gttgacggtt ctggttacca agtccacaga accatgcaat tcgaagatgg
     3841 tgcttccttg accgtgaact acagatacac ctacgaaggt tctcacatca agggcgaagc
     3901 ccaagttaag ggtaccggtt tccctgctga cggtccagtc atgactaact ccttgaccgc
     3961 tgctgactgg tgtcgttcca agaaaaccta cccaaacgat aagaccatca tttccacttt
     4021 caagtggtcc tacaccaccg gtaacggcaa gagataccgc tctaccgctc gtaccaccta
     4081 caccttcgcc aagccaatgg ctgccaacta cttgaagaac cagcctatgt acgttttccg
     4141 taagaccgag ctgaagcatt ccaagactga attgaacttc aaggaatggc aaaaggcttt
     4201 caccgacgtc atgggtatgg acgaattgta caagTCTGGT CCTTCTGGCC CAACTGGTTC
     4261 Ttgagaccga aagtgaaacg tgatttcatg cgtcattttg aacattttgt aaatcttatt
     4321 taataatgtg tgcggcaatt cacatttaat ttatgaatgt tttcttaaca tcgcggcaac
     4381 tcaagaaacg gcaggttcgg atcttagcta ctagagaaag aggagaaata ctagatgcgt
     4441 aaaggcgaag agctgttcac tggtgtcgtc cctattctgg tggaactgga tggtgatgtc
     4501 aacggtcata agttttccgt gcgtggcgag ggtgaaggtg acgcaactaa tggtaaactg
     4561 acgctgaagt tcatctgtac tactggtaaa ctgccggttc cttggccgac tctggtaacg
     4621 acgctgactt atggtgttca gtgctttgct cgttatccgg accatatgaa gcagcatgac
     4681 ttcttcaagt ccgccatgcc ggaaggctat gtgcaggaac gcacgatttc ctttaaggat
     4741 gacggcacgt acaaaacgcg tgcggaagtg aaatttgaag gcgataccct ggtaaaccgc
     4801 attgagctga aaggcattga ctttaaagag gacggcaata tcctgggcca taagctggaa
     4861 tacaatttta acagccacaa tgtttacatc accgccgata aacaaaaaaa tggcattaaa
     4921 gcgaatttta aaattcgcca caacgtggag gatggcagcg tgcagctggc tgatcactac
     4981 cagcaaaaca ctccaatcgg tgatggtcct gttctgctgc cagacaatca ctatctgagc
     5041 acgcaaagcg ttctgtctaa agatccgaac gagaaacgcg atcatatggt tctgctggag
     5101 ttcgtaaccg cagcgggcat cacgcatggt atggatgaac tgtacaaatg accaggcatc
     5161 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg
     5221 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata
     5281 ggtctcaATC Ctaactcgag gccaattggt gcggcaattg ataataacga aaatgtcttt
     5341 taatgatctg ggtataatga ggaattttcc gaacgttttt actttatata tatatataca
     5401 tgtaacatat attctatacg ctatagagaa aggaaatttt tcaattaaaa aaaaaataga
     5461 gaaagagttt cacttcttga ttatcgctaa cactaatggt tgaagtactg ctactttaat
     5521 tttatgctgc caatgagacg acggggtcat cacggctcat catgcgccaa acaaatgtgt
     5581 gcaatacacg ctcggatgac tgcatgatga ccgcactgac tggggacagc agatccacct
     5641 aagcctgtga gagaagcaga cacccgacag atcaaggcag ttaactagtg cactgcagTA
     5701 CAaatcgtcc ccaacaaaag tgggctctca aaattcatca catttaaatg catataggaa
     5761 gagcaacagt tggtttgcat ctgatgttcc ttaaagattt cgacataatg tgcgaagtag
     5821 atCCCTTCAG CACTGTACTT GTTTTTGCGA CTAGATTGTA AATCATTCTT TATTTAATCT
     5881 CTTTCTTTAA CTACTGCTTA AAGTATAATT TGGTCCGTAG TTTAATAACT ATACTAAGCG
     5941 AACGCTCTCT CGAAATTTTT CTTAACGCGT CCTTGTACTG CGTCTAACGC TTTTGCCACT
     6001 TGGATTTCTA TTATAGGAAA TAGTCTCACT TACTGGGCGA CGAATTTTCG CGTTTTGATG
     6061 AAGCACAGGA AGAATTTCTT TATGctcaga agtttgacag caagcaagtt catcattcga
     6121 actagcctta ttgttttagt tcagtgacag cgaactgccg tactcgatgc tttatttctc
     6181 acggtagagc ggaagaacag atagATCCCT ACATATTGCT ACCACTTCTA TTACACAATA
     6241 GTTTCAATAG CTTGCAGCGT AGCTAAACTC TAAAATTTAT CTAAATCACT CATATAAACC
     6301 GAACCCTTCC CCTTCCGCTT ATAGTACAGC TGATGCTCCT TCACTATTTT AACATGTGGA
     6361 ATTCTTGAAA GAATGAAATC GCCATGCCAA GCCATCACAC GGTCTTTTAT GCAATTGATT
     6421 GACCGCCTGC AACACATAGG CAGTAAAATT TTTACAAGCA GTAGCAGAAC AGGCCACACA
     6481 ATCGCAAGTG ATTAACGTCC ACACAGGTAT AGGGTTTCTG GACCATATGA TACATGCTCT
     6541 GGCCAAGCAT TCCGGCTGGT CGCTAATCGT TGAGTGccga gcggccgcga ttatcaaaaa
     6601 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     6661 atgagtaaac ttggtctgac ag
//