LOCUS       pBBK36_pHomL-RPL18Bp-GF 6672 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3521
                     /label="RPL18B promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3527..3530
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3528..3530
                     /label="Translation 3528-3530"
     misc_feature    3531..4556
                     /label="GFP dropout cassette"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     RBS             3686..3697
                     /label="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             3704..4417
                     /label="superfolder GFP"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     terminator      4429..4500
                     /label="rrnB T1 terminator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      4516..4543
                     /label="T7Te terminator"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4557..4560
                     /label="3>4"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4558..4581
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4558..4581
                     /label="Translation 4558-4581"
     ORF             4582..5277
                     /label="ymScarlet"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             4582..5280
                     /label="Translation 4582-5280"
     primer          4888..4909
                     /label="BB1186. Scarlet seq F"
                     /note="sequence: ggtggtgctgttactgttactc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5110..5133)
                     /label="BB1187. Scarlet seq R"
                     /note="sequence: agctttataggtggtcttgaagtc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5291..5515
                     /label="SSA1t"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(5469..5491)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5516..5519
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5520..5688
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5689..5692
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5693..5812
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5693..6566
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5791..5811)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5817..5930
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5935..6070
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6042..6062
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6075..6194
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6199..6318
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6296..6315)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6323..6442
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6447..6566
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6567..6570
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatcTATG tgagaccgaa
     3541 agtgaaacgt gatttcatgc gtcattttga acattttgta aatcttattt aataatgtgt
     3601 gcggcaattc acatttaatt tatgaatgtt ttcttaacat cgcggcaact caagaaacgg
     3661 caggttcgga tcttagctac tagagaaaga ggagaaatac tagatgcgta aaggcgaaga
     3721 gctgttcact ggtgtcgtcc ctattctggt ggaactggat ggtgatgtca acggtcataa
     3781 gttttccgtg cgtggcgagg gtgaaggtga cgcaactaat ggtaaactga cgctgaagtt
     3841 catctgtact actggtaaac tgccggttcc ttggccgact ctggtaacga cgctgactta
     3901 tggtgttcag tgctttgctc gttatccgga ccatatgaag cagcatgact tcttcaagtc
     3961 cgccatgccg gaaggctatg tgcaggaacg cacgatttcc tttaaggatg acggcacgta
     4021 caaaacgcgt gcggaagtga aatttgaagg cgataccctg gtaaaccgca ttgagctgaa
     4081 aggcattgac tttaaagagg acggcaatat cctgggccat aagctggaat acaattttaa
     4141 cagccacaat gtttacatca ccgccgataa acaaaaaaat ggcattaaag cgaattttaa
     4201 aattcgccac aacgtggagg atggcagcgt gcagctggct gatcactacc agcaaaacac
     4261 tccaatcggt gatggtcctg ttctgctgcc agacaatcac tatctgagca cgcaaagcgt
     4321 tctgtctaaa gatccgaacg agaaacgcga tcatatggtt ctgctggagt tcgtaaccgc
     4381 agcgggcatc acgcatggta tggatgaact gtacaaatga ccaggcatca aataaaacga
     4441 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     4501 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatag gtctcaATCC
     4561 GGTCCTTCTG GCCCAACTGG Catggttagt aaaggtgaag ctgttataaa agaattcatg
     4621 aggtttaaag ttcatatgga aggttcaatg aatggtcatg aatttgaaat tgaaggtgaa
     4681 ggtgaaggta gaccatatga aggtacacaa actgctaaat tgaaagttac taaaggtggt
     4741 ccattaccat tttcttggga tattttgtct ccacaattca tgtatggttc tagagctttt
     4801 attaagcatc cagctgatat tccagattat tataaacaat cttttcctga aggttttaaa
     4861 tgggaaagag ttatgaattt cgaagatggt ggtgctgtta ctgttactca agatacttct
     4921 ttggaagatg gtactttaat ctataaagtt aaattgagag gtactaattt tccaccagat
     4981 ggtccagtta tgcaaaagaa aactatgggt tgggaagcat ctactgaaag attgtatcca
     5041 gaagatggtg ttttgaaagg agacattaag atggctttga gattgaaaga tggtggtcgg
     5101 tacctggccg acttcaagac cacctataaa gctaaaaaac cagttcaaat gccaggtgca
     5161 tataatgttg atagaaagtt agatataacg tcgcataacg aggactatac tgtagttgaa
     5221 caatatgaac gtagtgaagg tagacatagt accggaggaa tggatgaatt gtataaaTAG
     5281 ctcgagtggc gccaattggt gcggcaattg ataataacga aaatgtcttt taatgatctg
     5341 ggtataatga ggaattttcc gaacgttttt actttatata tatatataca tgtaacatat
     5401 attctatacg ctatagagaa aggaaatttt tcaattaaaa aaaaaataga gaaagagttt
     5461 cacttcttga ttatcgctaa cactaatggt tgaagtactg ctactttaat tttatgctgc
     5521 caatgagacg acggggtcat cacggctcat catgcgccaa acaaatgtgt gcaatacacg
     5581 ctcggatgac tgcatgatga ccgcactgac tggggacagc agatccacct aagcctgtga
     5641 gagaagcaga cacccgacag atcaaggcag ttaactagtg cactgcagTA CAaatcgtcc
     5701 ccaacaaaag tgggctctca aaattcatca catttaaatg catataggaa gagcaacagt
     5761 tggtttgcat ctgatgttcc ttaaagattt cgacataatg tgcgaagtag atCCCTTCAG
     5821 CACTGTACTT GTTTTTGCGA CTAGATTGTA AATCATTCTT TATTTAATCT CTTTCTTTAA
     5881 CTACTGCTTA AAGTATAATT TGGTCCGTAG TTTAATAACT ATACTAAGCG AACGCTCTCT
     5941 CGAAATTTTT CTTAACGCGT CCTTGTACTG CGTCTAACGC TTTTGCCACT TGGATTTCTA
     6001 TTATAGGAAA TAGTCTCACT TACTGGGCGA CGAATTTTCG CGTTTTGATG AAGCACAGGA
     6061 AGAATTTCTT TATGctcaga agtttgacag caagcaagtt catcattcga actagcctta
     6121 ttgttttagt tcagtgacag cgaactgccg tactcgatgc tttatttctc acggtagagc
     6181 ggaagaacag atagATCCCT ACATATTGCT ACCACTTCTA TTACACAATA GTTTCAATAG
     6241 CTTGCAGCGT AGCTAAACTC TAAAATTTAT CTAAATCACT CATATAAACC GAACCCTTCC
     6301 CCTTCCGCTT ATAGTACAGC TGATGCTCCT TCACTATTTT AACATGTGGA ATTCTTGAAA
     6361 GAATGAAATC GCCATGCCAA GCCATCACAC GGTCTTTTAT GCAATTGATT GACCGCCTGC
     6421 AACACATAGG CAGTAAAATT TTTACAAGCA GTAGCAGAAC AGGCCACACA ATCGCAAGTG
     6481 ATTAACGTCC ACACAGGTAT AGGGTTTCTG GACCATATGA TACATGCTCT GGCCAAGCAT
     6541 TCCGGCTGGT CGCTAATCGT TGAGTGccga gcggccgcga ttatcaaaaa ggatcttcac
     6601 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6661 ttggtctgac ag
//