LOCUS       pBBK53_pHomL-RPL18Bp-Ne 6309 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(633..652)
                     /label="BB1006. AmpR seq1 R"
                     /note="sequence: taaagttctgctatgtggcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3521
                     /label="RPL18B promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3527..3527
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3528..4235
                     /label="ymNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3528..4262
                     /label="Translation 3528-4262"
     primer          complement(3617..3634)
                     /label="BB1179. mNeon R"
                     /note="sequence: ccttgaccgaccatatcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3804..3824
                     /label="BB899. NeonGreen seq F"
                     /note="sequence: ggttaccaagtccacagaacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4236..4262
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4263..4907
                     /label="SFT2"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4263..4910
                     /label="Translation 4263-4910"
     primer          4632..4650
                     /label="BB958. SFT2 seq1 F"
                     /note="sequence: GTTCTTGCGTTTGGGGTAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4919..5152
                     /label="SSA1t (4)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5106..5128)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5153..5156
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5157..5325
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5326..5329
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5330..5449
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5330..6203
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5428..5448)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5454..5567
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5572..5707
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5679..5699
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5712..5831
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5836..5955
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(5933..5952)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5960..6079
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6084..6203
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6204..6207
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatcTatg gtttctaagg
     3541 gtgaagaaga taacatggct tctttgccag ccacccacga gttgcacatc ttcggttcca
     3601 ttaacggtgt cgacttcgat atggtcggtc aaggtaccgg taacccaaac gacggttacg
     3661 aagaattgaa cttgaagtct accaagggcg acttgcagtt ctccccttgg attttggttc
     3721 cacacatcgg ttacggtttc caccaatact tgccataccc agacggtatg tccccattcc
     3781 aagccgccat ggttgacggt tctggttacc aagtccacag aaccatgcaa ttcgaagatg
     3841 gtgcttcctt gaccgtgaac tacagataca cctacgaagg ttctcacatc aagggcgaag
     3901 cccaagttaa gggtaccggt ttccctgctg acggtccagt catgactaac tccttgaccg
     3961 ctgctgactg gtgtcgttcc aagaaaacct acccaaacga taagaccatc atttccactt
     4021 tcaagtggtc ctacaccacc ggtaacggca agagataccg ctctaccgct cgtaccacct
     4081 acaccttcgc caagccaatg gctgccaact acttgaagaa ccagcctatg tacgttttcc
     4141 gtaagaccga gctgaagcat tccaagactg aattgaactt caaggaatgg caaaaggctt
     4201 tcaccgacgt catgggtatg gacgaattgt acaagTCTGG TCCTTCTGGC CCAACTGGTT
     4261 CTATGAGCGA GGAACCACCT TCTGACCAGG TCAATAGTCT CCGTGACTCA TTGAATCGAT
     4321 GGAATCAAAC AAGACAGCAG AACTCGCAGG GTTTTAATGA ATCTGCGAAG ACATTGTTCT
     4381 CAAGCTGGGC GGATTCTCTC AATACCAGGG CCCAGGATAT ATATCAGACG TTGCCTGTAT
     4441 CTAGACAGGA CTTGGTGCAA GACCAGGAGC CGTCGTGGTT CCAATTGTCA AGAACGGAAA
     4501 GAATGGTACT TTTTGTCTGT TTTCTTTTGG GTGCAACAGC CTGTTTCACT CTTTGTACTT
     4561 TCCTTTTCCC CGTTCTAGCC GCTAAACCAA GAAAGTTTGG TTTACTATGG ACAATGGGGT
     4621 CCCTACTATT TGTTCTTGCG TTTGGGGTAC TTATGGGACC ACTCGCGTAC TTAAAACATT
     4681 TGACTGCAAG GGAAAGGCTG CCTTTTTCGA TGTTCTTTTT CGCCACATGC TTCATGACGA
     4741 TTTATTTCGC AGCCTTTTCC AAGAACACGG TGCTGACTAT TACATGTGCT CTTCTTGAAT
     4801 TAGTTGCCGT CATTTATTAT GCTATTTCAT ATTTCCCATT CGGTGCAACA GGTTTGAGGA
     4861 TGTTAAGCTC TGCTGGTGTC AATTCGGCAA GAGGTGTTCT GCGCATCTAG cgagATCCta
     4921 actcgaggcc aattggtgcg gcaattgata ataacgaaaa tgtcttttaa tgatctgggt
     4981 ataatgagga attttccgaa cgtttttact ttatatatat atatacatgt aacatatatt
     5041 ctatacgcta tagagaaagg aaatttttca attaaaaaaa aaatagagaa agagtttcac
     5101 ttcttgatta tcgctaacac taatggttga agtactgcta ctttaatttt atgctgccaa
     5161 tgagacgacg gggtcatcac ggctcatcat gcgccaaaca aatgtgtgca atacacgctc
     5221 ggatgactgc atgatgaccg cactgactgg ggacagcaga tccacctaag cctgtgagag
     5281 aagcagacac ccgacagatc aaggcagtta actagtgcac tgcagTACAa atcgtcccca
     5341 acaaaagtgg gctctcaaaa ttcatcacat ttaaatgcat ataggaagag caacagttgg
     5401 tttgcatctg atgttcctta aagatttcga cataatgtgc gaagtagatC CCTTCAGCAC
     5461 TGTACTTGTT TTTGCGACTA GATTGTAAAT CATTCTTTAT TTAATCTCTT TCTTTAACTA
     5521 CTGCTTAAAG TATAATTTGG TCCGTAGTTT AATAACTATA CTAAGCGAAC GCTCTCTCGA
     5581 AATTTTTCTT AACGCGTCCT TGTACTGCGT CTAACGCTTT TGCCACTTGG ATTTCTATTA
     5641 TAGGAAATAG TCTCACTTAC TGGGCGACGA ATTTTCGCGT TTTGATGAAG CACAGGAAGA
     5701 ATTTCTTTAT Gctcagaagt ttgacagcaa gcaagttcat cattcgaact agccttattg
     5761 ttttagttca gtgacagcga actgccgtac tcgatgcttt atttctcacg gtagagcgga
     5821 agaacagata gATCCCTACA TATTGCTACC ACTTCTATTA CACAATAGTT TCAATAGCTT
     5881 GCAGCGTAGC TAAACTCTAA AATTTATCTA AATCACTCAT ATAAACCGAA CCCTTCCCCT
     5941 TCCGCTTATA GTACAGCTGA TGCTCCTTCA CTATTTTAAC ATGTGGAATT CTTGAAAGAA
     6001 TGAAATCGCC ATGCCAAGCC ATCACACGGT CTTTTATGCA ATTGATTGAC CGCCTGCAAC
     6061 ACATAGGCAG TAAAATTTTT ACAAGCAGTA GCAGAACAGG CCACACAATC GCAAGTGATT
     6121 AACGTCCACA CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC CAAGCATTCC
     6181 GGCTGGTCGC TAATCGTTGA GTGccgagcg gccgcgatta tcaaaaagga tcttcaccta
     6241 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6301 gtctgacag
//