LOCUS       pBBK62_pHomL-RPL18Bp-ER 6806 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3521
                     /label="RPL18B promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3528..4676
                     /label="ERG6"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3528..4676
                     /label="Translation 3528-4676"
     primer          4077..4098
                     /label="BB313. ERG6 int Chk F"
                     /note="sequence: GAAGAAAACACTTTCGACAAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4677..4682
                     /label="Translation 4677-4682"
     misc_feature    4683..4703
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4683..4703
                     /label="Translation 4683-4703"
     misc_feature    4704..5414
                     /label="ymNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4704..5414
                     /label="Translation 4704-5414"
     primer          complement(4793..4810)
                     /label="BB1179. mNeon R"
                     /note="sequence: ccttgaccgaccatatcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          4980..5000
                     /label="BB899. NeonGreen seq F"
                     /note="sequence: ggttaccaagtccacagaacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5421..5424
                     /label="4a>4b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5425..5649
                     /label="SSA1t"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(5603..5625)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5650..5653
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5654..5822
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5823..5826
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5827..5946
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5827..6700
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5925..5945)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5951..6064
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6069..6204
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6176..6196
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6209..6328
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6333..6452
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6430..6449)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6457..6576
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6581..6700
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6701..6704
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatcTATG AGTGAAACAG
     3541 AATTGAGAAA AAGACAGGCC CAATTCACTA GGGAGTTACA TGGTGATGAT ATTGGTAAAA
     3601 AGACAGGTTT GAGTGCATTG ATGTCGAAGA ACAACTCTGC CCAAAAGGAA GCCGTTCAGA
     3661 AGTACTTGAG AAATTGGGAT GGTAGAACCG ATAAAGATGC CGAAGAACGT CGTCTTGAGG
     3721 ATTATAATGA AGCCACACAT TCCTACTATA ACGTCGTTAC AGATTTCTAT GAATATGGTT
     3781 GGGGTTCCTC TTTCCATTTC AGCAGATTTT ATAAAGGTGA GAGTTTCGCT GCCTCGATAG
     3841 CAAGACATGA ACATTATTTA GCTTACAAGG CTGGTATTCA AAGAGGCGAT TTAGTTCTCG
     3901 ACGTTGGTTG TGGTGTTGGG GGCCCAGCAA GAGAGATTGC AAGATTTACC GGTTGTAACG
     3961 TCATCGGTCT AAACAATAAC GATTACCAAA TTGCCAAGGC AAAATATTAC GCTAAAAAAT
     4021 ACAATTTGAG TGACCAAATG GACTTTGTAA AGGGTGATTT CATGAAAATG GATTTCGAAG
     4081 AAAACACTTT CGACAAAGTT TATGCAATTG AGGCCACATG TCACGCTCCA AAATTAGAAG
     4141 GTGTATACAG CGAAATCTAC AAGGTTTTGA AACCGGGTGG TACCTTTGCT GTTTACGAAT
     4201 GGGTAATGAC TGATAAATAT GACGAAAACA ATCCTGAACA TAGAAAGATC GCTTATGAAA
     4261 TTGAACTAGG TGATGGTATC CCAAAGATGT TCCATGTCGA CGTGGCTAGG AAAGCATTGA
     4321 AGAACTGTGG TTTCGAAGTC CTCGTTAGCG AAGACCTGGC GGACAATGAT GATGAAATCC
     4381 CTTGGTATTA CCCATTAACT GGTGAGTGGA AGTACGTTCA AAACTTAGCT AATTTGGCCA
     4441 CATTTTTCAG AACTTCTTAC TTGGGTAGAC AATTTACTAC AGCAATGGTT ACTGTAATGG
     4501 AAAAATTAGG TCTAGCCCCA GAAGGTTCCA AGGAAGTTAC TGCTGCTCTA GAAAATGCTG
     4561 CGGTTGGTTT AGTTGCCGGT GGTAAGTCCA AGTTATTCAC TCCAATGATG CTTTTCGTCG
     4621 CTAGGAAGCC AGAAAACGCC GAAACCCCCT CCCAAACTTC CCAAGAAGCA ACTCAAGGAT
     4681 CCGGTCCTTC TGGCCCAACT GGCatggttt ctaagggtga agaagataac atggcttctt
     4741 tgccagccac ccacgagttg cacatcttcg gttccattaa cggtgtcgac ttcgatatgg
     4801 tcggtcaagg taccggtaac ccaaacgacg gttacgaaga attgaacttg aagtctacca
     4861 agggcgactt gcagttctcc ccttggattt tggttccaca catcggttac ggtttccacc
     4921 aatacttgcc atacccagac ggtatgtccc cattccaagc cgccatggtt gacggttctg
     4981 gttaccaagt ccacagaacc atgcaattcg aagatggtgc ttccttgacc gtgaactaca
     5041 gatacaccta cgaaggttct cacatcaagg gcgaagccca agttaagggt accggtttcc
     5101 ctgctgacgg tccagtcatg actaactcct tgaccgctgc tgactggtgt cgttccaaga
     5161 aaacctaccc aaacgataag accatcattt ccactttcaa gtggtcctac accaccggta
     5221 acggcaagag ataccgctct accgctcgta ccacctacac cttcgccaag ccaatggctg
     5281 ccaactactt gaagaaccag cctatgtacg ttttccgtaa gaccgagctg aagcattcca
     5341 agactgaatt gaacttcaag gaatggcaaa aggctttcac cgacgtcatg ggtatggacg
     5401 aattgtacaa gtaactcgag tggcgccaat tggtgcggca attgataata acgaaaatgt
     5461 cttttaatga tctgggtata atgaggaatt ttccgaacgt ttttacttta tatatatata
     5521 tacatgtaac atatattcta tacgctatag agaaaggaaa tttttcaatt aaaaaaaaaa
     5581 tagagaaaga gtttcacttc ttgattatcg ctaacactaa tggttgaagt actgctactt
     5641 taattttatg ctgccaatga gacgacgggg tcatcacggc tcatcatgcg ccaaacaaat
     5701 gtgtgcaata cacgctcgga tgactgcatg atgaccgcac tgactgggga cagcagatcc
     5761 acctaagcct gtgagagaag cagacacccg acagatcaag gcagttaact agtgcactgc
     5821 agTACAaatc gtccccaaca aaagtgggct ctcaaaattc atcacattta aatgcatata
     5881 ggaagagcaa cagttggttt gcatctgatg ttccttaaag atttcgacat aatgtgcgaa
     5941 gtagatCCCT TCAGCACTGT ACTTGTTTTT GCGACTAGAT TGTAAATCAT TCTTTATTTA
     6001 ATCTCTTTCT TTAACTACTG CTTAAAGTAT AATTTGGTCC GTAGTTTAAT AACTATACTA
     6061 AGCGAACGCT CTCTCGAAAT TTTTCTTAAC GCGTCCTTGT ACTGCGTCTA ACGCTTTTGC
     6121 CACTTGGATT TCTATTATAG GAAATAGTCT CACTTACTGG GCGACGAATT TTCGCGTTTT
     6181 GATGAAGCAC AGGAAGAATT TCTTTATGct cagaagtttg acagcaagca agttcatcat
     6241 tcgaactagc cttattgttt tagttcagtg acagcgaact gccgtactcg atgctttatt
     6301 tctcacggta gagcggaaga acagatagAT CCCTACATAT TGCTACCACT TCTATTACAC
     6361 AATAGTTTCA ATAGCTTGCA GCGTAGCTAA ACTCTAAAAT TTATCTAAAT CACTCATATA
     6421 AACCGAACCC TTCCCCTTCC GCTTATAGTA CAGCTGATGC TCCTTCACTA TTTTAACATG
     6481 TGGAATTCTT GAAAGAATGA AATCGCCATG CCAAGCCATC ACACGGTCTT TTATGCAATT
     6541 GATTGACCGC CTGCAACACA TAGGCAGTAA AATTTTTACA AGCAGTAGCA GAACAGGCCA
     6601 CACAATCGCA AGTGATTAAC GTCCACACAG GTATAGGGTT TCTGGACCAT ATGATACATG
     6661 CTCTGGCCAA GCATTCCGGC TGGTCGCTAA TCGTTGAGTG ccgagcggcc gcgattatca
     6721 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6781 atatatgagt aaacttggtc tgacag
//