LOCUS       pBBK64_pHomL-RPL18Bp-PE 6839 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3521
                     /label="RPL18B promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3528..4709
                     /label="PEX25"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             3528..4709
                     /label="Translation 3528-4709"
     primer          4063..4081
                     /label="BB929. PEX25 seq1 F"
                     /note="sequence: GAGTGTGCAGTTTCTTGGG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4710..4715
                     /label="Translation 4710-4715"
     misc_feature    4716..4736
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             4716..4736
                     /label="Translation 4716-4736"
     misc_feature    4737..5447
                     /label="ymNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             4737..5447
                     /label="Translation 4737-5447"
     primer          complement(4826..4843)
                     /label="BB1179. mNeon R"
                     /note="sequence: ccttgaccgaccatatcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5013..5033
                     /label="BB899. NeonGreen seq F"
                     /note="sequence: ggttaccaagtccacagaacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5454..5457
                     /label="4a>4b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5458..5682
                     /label="SSA1t"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(5636..5658)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          5636..5659
                     /label="BB1353. SSA1t F"
                     /note="sequence: gattatcgctaacactaatggttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5683..5686
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5687..5855
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5856..5859
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5860..5979
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5860..6733
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5958..5978)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5984..6097
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6102..6237
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6209..6229
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6242..6361
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6366..6485
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6463..6482)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6490..6609
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6614..6733
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6734..6737
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatcTATG AGTCAGTTTG
     3541 GCACGACAGA TATCGTTTCC GGTTCAGAAA CACCACCTTA TTCTGGGGCA AGTTACCAGG
     3601 ACGCCCAGGA TGACAATACC CATCCACATT CATCAGACGC CGGTGCAGAG AAGTTCAGTG
     3661 CAGGCAGTGG AAGCGAGTCG CATACTGAAT CCTCGAGAAG TGATGATGAG GATTCTCAAG
     3721 CAAAAACAAA GATGGTGGAT AATATTACCA TCTTAAAATA TATATTGGAT TCTCTATCTG
     3781 GTAGAGACAA ATTAGCCAAA ATTATCAAAT ACGCTCTGGA TATATTAAAA CTGTTTATCG
     3841 AAAAGTCTAA GCGAAATTTA ACTGTTTTGG ACCCTTCCGT ATTAACGTAC TATACCAAGA
     3901 TTTTGAAAAA TCTGACTGTA AAAGTCGCAC TAAGGCATCC AATCACGGTA ATTAAAGTAC
     3961 TTCTCCTGTC TCTTTTGAGA AATTTTGACA AGAAAATTGA TTTTATCAGT CAACAATTAA
     4021 GTACTTTTAG ATATATTTTA AGGTTTGGCG GCACACCATT CCGAGTGTGC AGTTTCTTGG
     4081 GGAAATTCAA TAAAACCAGA AAGTGCAACT TTCAAATTGA CCAAATTAAG AAAATATGGT
     4141 TTAATGAAGC TTCATTGAGA GAATTTTTGG ACTTGTATTA TGGTATCTTT GATGAATTAG
     4201 ACTTGCTATA CAAATTGAAA ATATGGACAA ATAAATCATT CTATTCCTTT GTCAGTAGAC
     4261 AAGAGTCTCT TGCATGGCAA TACGACATTC TTTTAAGCTT GAAGGACCAT TGGCTCAACC
     4321 TGCAAAGCTT ACAGAAGAGA CAATTAGAAT TGGAAGTGCA ACTAAAAGTT CAAAATAACG
     4381 CGTTACTACT CTCACCCATA CTGATGCATC AGGCCCATAA AGACGATGGC TCACAATCGC
     4441 CAATTAGGAA ACAGTTGTTA AATGACCTAA ATGTTAATAA CGACGCAGAG GTGTTAATAC
     4501 ACAAACAACT AAAGGCCATC AAGGATGAAA AGACACTTGT TTATTTAGAT ATTGCAAGAT
     4561 TATCGTTTGA TTGCATGGCT AATACATCAG ATATTCTAAA TTTAAAAACT CCAAAGGGAA
     4621 CGTATGCCGT TCTGTCCCTG GGATCCGGCT TAACTGGACT GGTTAAACTT TGGATAACAA
     4681 CAAAGAGGTC ACTTTGCTCT TCAAAAGATG GATCCGGTCC TTCTGGCCCA ACTGGCatgg
     4741 tttctaaggg tgaagaagat aacatggctt ctttgccagc cacccacgag ttgcacatct
     4801 tcggttccat taacggtgtc gacttcgata tggtcggtca aggtaccggt aacccaaacg
     4861 acggttacga agaattgaac ttgaagtcta ccaagggcga cttgcagttc tccccttgga
     4921 ttttggttcc acacatcggt tacggtttcc accaatactt gccataccca gacggtatgt
     4981 ccccattcca agccgccatg gttgacggtt ctggttacca agtccacaga accatgcaat
     5041 tcgaagatgg tgcttccttg accgtgaact acagatacac ctacgaaggt tctcacatca
     5101 agggcgaagc ccaagttaag ggtaccggtt tccctgctga cggtccagtc atgactaact
     5161 ccttgaccgc tgctgactgg tgtcgttcca agaaaaccta cccaaacgat aagaccatca
     5221 tttccacttt caagtggtcc tacaccaccg gtaacggcaa gagataccgc tctaccgctc
     5281 gtaccaccta caccttcgcc aagccaatgg ctgccaacta cttgaagaac cagcctatgt
     5341 acgttttccg taagaccgag ctgaagcatt ccaagactga attgaacttc aaggaatggc
     5401 aaaaggcttt caccgacgtc atgggtatgg acgaattgta caagtaactc gagtggcgcc
     5461 aattggtgcg gcaattgata ataacgaaaa tgtcttttaa tgatctgggt ataatgagga
     5521 attttccgaa cgtttttact ttatatatat atatacatgt aacatatatt ctatacgcta
     5581 tagagaaagg aaatttttca attaaaaaaa aaatagagaa agagtttcac ttcttgatta
     5641 tcgctaacac taatggttga agtactgcta ctttaatttt atgctgccaa tgagacgacg
     5701 gggtcatcac ggctcatcat gcgccaaaca aatgtgtgca atacacgctc ggatgactgc
     5761 atgatgaccg cactgactgg ggacagcaga tccacctaag cctgtgagag aagcagacac
     5821 ccgacagatc aaggcagtta actagtgcac tgcagTACAa atcgtcccca acaaaagtgg
     5881 gctctcaaaa ttcatcacat ttaaatgcat ataggaagag caacagttgg tttgcatctg
     5941 atgttcctta aagatttcga cataatgtgc gaagtagatC CCTTCAGCAC TGTACTTGTT
     6001 TTTGCGACTA GATTGTAAAT CATTCTTTAT TTAATCTCTT TCTTTAACTA CTGCTTAAAG
     6061 TATAATTTGG TCCGTAGTTT AATAACTATA CTAAGCGAAC GCTCTCTCGA AATTTTTCTT
     6121 AACGCGTCCT TGTACTGCGT CTAACGCTTT TGCCACTTGG ATTTCTATTA TAGGAAATAG
     6181 TCTCACTTAC TGGGCGACGA ATTTTCGCGT TTTGATGAAG CACAGGAAGA ATTTCTTTAT
     6241 Gctcagaagt ttgacagcaa gcaagttcat cattcgaact agccttattg ttttagttca
     6301 gtgacagcga actgccgtac tcgatgcttt atttctcacg gtagagcgga agaacagata
     6361 gATCCCTACA TATTGCTACC ACTTCTATTA CACAATAGTT TCAATAGCTT GCAGCGTAGC
     6421 TAAACTCTAA AATTTATCTA AATCACTCAT ATAAACCGAA CCCTTCCCCT TCCGCTTATA
     6481 GTACAGCTGA TGCTCCTTCA CTATTTTAAC ATGTGGAATT CTTGAAAGAA TGAAATCGCC
     6541 ATGCCAAGCC ATCACACGGT CTTTTATGCA ATTGATTGAC CGCCTGCAAC ACATAGGCAG
     6601 TAAAATTTTT ACAAGCAGTA GCAGAACAGG CCACACAATC GCAAGTGATT AACGTCCACA
     6661 CAGGTATAGG GTTTCTGGAC CATATGATAC ATGCTCTGGC CAAGCATTCC GGCTGGTCGC
     6721 TAATCGTTGA GTGccgagcg gccgcgatta tcaaaaagga tcttcaccta gatcctttta
     6781 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacag
//