LOCUS       pBBK65_pHomL-RPL18Bp-pr 5738 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3521
                     /label="RPL18B promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3528..3608
                     /label="preCOX4"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             3528..3608
                     /label="Translation 3528-3608"
     CDS             3609..3614
                     /label="Translation 3609-3614"
     misc_feature    3615..3635
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             3615..3635
                     /label="Translation 3615-3635"
     misc_feature    3636..4346
                     /label="ymNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3636..4346
                     /label="Translation 3636-4346"
     primer          complement(3725..3742)
                     /label="BB1179. mNeon R"
                     /note="sequence: ccttgaccgaccatatcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3912..3932
                     /label="BB899. NeonGreen seq F"
                     /note="sequence: ggttaccaagtccacagaacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4353..4356
                     /label="4a>4b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4357..4581
                     /label="SSA1t"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          complement(4535..4557)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4582..4585
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4586..4754
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4755..4758
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4759..4878
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4759..5632
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(4857..4877)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4883..4996
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5001..5136
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5108..5128
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5141..5260
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5265..5384
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(5362..5381)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5389..5508
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5513..5632
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5633..5636
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatcTATG CTTTCACTAC
     3541 GTCAATCTAT AAGATTTTTC AAGCCAGCCA CAAGAACTTT GTGTAGCTCT AGATATCTGC
     3601 TTCAGCAAGG ATCCGGTCCT TCTGGCCCAA CTGGCatggt ttctaagggt gaagaagata
     3661 acatggcttc tttgccagcc acccacgagt tgcacatctt cggttccatt aacggtgtcg
     3721 acttcgatat ggtcggtcaa ggtaccggta acccaaacga cggttacgaa gaattgaact
     3781 tgaagtctac caagggcgac ttgcagttct ccccttggat tttggttcca cacatcggtt
     3841 acggtttcca ccaatacttg ccatacccag acggtatgtc cccattccaa gccgccatgg
     3901 ttgacggttc tggttaccaa gtccacagaa ccatgcaatt cgaagatggt gcttccttga
     3961 ccgtgaacta cagatacacc tacgaaggtt ctcacatcaa gggcgaagcc caagttaagg
     4021 gtaccggttt ccctgctgac ggtccagtca tgactaactc cttgaccgct gctgactggt
     4081 gtcgttccaa gaaaacctac ccaaacgata agaccatcat ttccactttc aagtggtcct
     4141 acaccaccgg taacggcaag agataccgct ctaccgctcg taccacctac accttcgcca
     4201 agccaatggc tgccaactac ttgaagaacc agcctatgta cgttttccgt aagaccgagc
     4261 tgaagcattc caagactgaa ttgaacttca aggaatggca aaaggctttc accgacgtca
     4321 tgggtatgga cgaattgtac aagtaactcg agtggcgcca attggtgcgg caattgataa
     4381 taacgaaaat gtcttttaat gatctgggta taatgaggaa ttttccgaac gtttttactt
     4441 tatatatata tatacatgta acatatattc tatacgctat agagaaagga aatttttcaa
     4501 ttaaaaaaaa aatagagaaa gagtttcact tcttgattat cgctaacact aatggttgaa
     4561 gtactgctac tttaatttta tgctgccaat gagacgacgg ggtcatcacg gctcatcatg
     4621 cgccaaacaa atgtgtgcaa tacacgctcg gatgactgca tgatgaccgc actgactggg
     4681 gacagcagat ccacctaagc ctgtgagaga agcagacacc cgacagatca aggcagttaa
     4741 ctagtgcact gcagTACAaa tcgtccccaa caaaagtggg ctctcaaaat tcatcacatt
     4801 taaatgcata taggaagagc aacagttggt ttgcatctga tgttccttaa agatttcgac
     4861 ataatgtgcg aagtagatCC CTTCAGCACT GTACTTGTTT TTGCGACTAG ATTGTAAATC
     4921 ATTCTTTATT TAATCTCTTT CTTTAACTAC TGCTTAAAGT ATAATTTGGT CCGTAGTTTA
     4981 ATAACTATAC TAAGCGAACG CTCTCTCGAA ATTTTTCTTA ACGCGTCCTT GTACTGCGTC
     5041 TAACGCTTTT GCCACTTGGA TTTCTATTAT AGGAAATAGT CTCACTTACT GGGCGACGAA
     5101 TTTTCGCGTT TTGATGAAGC ACAGGAAGAA TTTCTTTATG ctcagaagtt tgacagcaag
     5161 caagttcatc attcgaacta gccttattgt tttagttcag tgacagcgaa ctgccgtact
     5221 cgatgcttta tttctcacgg tagagcggaa gaacagatag ATCCCTACAT ATTGCTACCA
     5281 CTTCTATTAC ACAATAGTTT CAATAGCTTG CAGCGTAGCT AAACTCTAAA ATTTATCTAA
     5341 ATCACTCATA TAAACCGAAC CCTTCCCCTT CCGCTTATAG TACAGCTGAT GCTCCTTCAC
     5401 TATTTTAACA TGTGGAATTC TTGAAAGAAT GAAATCGCCA TGCCAAGCCA TCACACGGTC
     5461 TTTTATGCAA TTGATTGACC GCCTGCAACA CATAGGCAGT AAAATTTTTA CAAGCAGTAG
     5521 CAGAACAGGC CACACAATCG CAAGTGATTA ACGTCCACAC AGGTATAGGG TTTCTGGACC
     5581 ATATGATACA TGCTCTGGCC AAGCATTCCG GCTGGTCGCT AATCGTTGAG TGccgagcgg
     5641 ccgcgattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5701 tcaatctaaa gtatatatga gtaaacttgg tctgacag
//