LOCUS       pBBK70_pHomL-RPL18Bp-Sc 6993 bp ds-DNA     circular     23-SEP-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(633..652)
                     /label="BB1006. AmpR seq1 R"
                     /note="sequence: taaagttctgctatgtggcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3521
                     /label="RPL18B promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    3527..3527
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     ORF             3528..4223
                     /label="ymScarlet"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3528..4250
                     /label="Translation 3528-4250"
     primer          3834..3855
                     /label="BB1186. Scarlet seq F"
                     /note="sequence: ggtggtgctgttactgttactc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4056..4079)
                     /label="BB1187. Scarlet seq R"
                     /note="sequence: agctttataggtggtcttgaagtc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4224..4250
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4251..5591
                     /label="TUB1"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     CDS             4251..5594
                     /label="Translation 4251-5594"
     primer          4276..4293
                     /label="BB1364. TUB1 clean F"
                     /note="sequence: TCGGTCAAGCTGGTTGTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(5212..5231)
                     /label="BB1367. TUB1 chk R"
                     /note="sequence: TCTTGTTACCACATCACCCC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5603..5836
                     /label="SSA1t (4)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5790..5812)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5837..5840
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5841..6009
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    6010..6013
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6014..6133
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6014..6887
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(6112..6132)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6138..6251
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6256..6391
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6363..6383
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6396..6515
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6520..6639
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6617..6636)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6644..6763
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6768..6887
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6888..6891
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gaagaggatg tccaatattt tttttaagga ataaggatac ttcaagacta gattcccccc
     2881 tgcattccca tcagaaccgt aaaccttggc gctttccttg ggaagtattc aagaagtgcc
     2941 ttgtccggtt tctgtggctc acaaaccagc gcgcccgata tggctttctt ttcacttatg
     3001 aatgtaccag tacgggacaa ttagaacgct cctgtaacaa tctctttgca aatgtggggt
     3061 tacattctaa ccatgtcaca ctgctgacga aattcaaagt aaaaaaaaat gggaccacgt
     3121 cttgagaacg atagattttc tttattttac attgaacagt cgttgtctca gcgcgcttta
     3181 tgttttcatt catacttcat attataaaat aacaaaagaa gaatttcata ttcacgccca
     3241 agaaatcagg ctgctttcca aatgcaattg acacttcatt agccatcaca caaaactctt
     3301 tcttgctgga gcttctttta aaaaagacct cagtacacca aacacgttac ccgacctcgt
     3361 tattttacga caactatgat aaaattctga agaaaaaata aaaaaatttt catacttctt
     3421 gcttttattt aaaccattga atgatttctt ttgaacaaaa ctacctgttt caccaaagga
     3481 aatagaaaga aaaaatcaat tagaagaaaa caaaaaacaa aagatcTatg gttagtaaag
     3541 gtgaagctgt tataaaagaa ttcatgaggt ttaaagttca tatggaaggt tcaatgaatg
     3601 gtcatgaatt tgaaattgaa ggtgaaggtg aaggtagacc atatgaaggt acacaaactg
     3661 ctaaattgaa agttactaaa ggtggtccat taccattttc ttgggatatt ttgtctccac
     3721 aattcatgta tggttctaga gcttttatta agcatccagc tgatattcca gattattata
     3781 aacaatcttt tcctgaaggt tttaaatggg aaagagttat gaatttcgaa gatggtggtg
     3841 ctgttactgt tactcaagat acttctttgg aagatggtac tttaatctat aaagttaaat
     3901 tgagaggtac taattttcca ccagatggtc cagttatgca aaagaaaact atgggttggg
     3961 aagcatctac tgaaagattg tatccagaag atggtgtttt gaaaggagac attaagatgg
     4021 ctttgagatt gaaagatggt ggtcggtacc tggccgactt caagaccacc tataaagcta
     4081 aaaaaccagt tcaaatgcca ggtgcatata atgttgatag aaagttagat ataacgtcgc
     4141 ataacgagga ctatactgta gttgaacaat atgaacgtag tgaaggtaga catagtaccg
     4201 gaggaatgga tgaattgtat aaaTCTGGTC CTTCTGGCCC AACTGGTTCT ATGAGAGAAG
     4261 TTATTAGTAT TAATGTCGGT CAAGCTGGTT GTCAGATTGG TAATGCCTGT TGGGAATTAT
     4321 ATTCCCTTGA GCACGGTATT AAGCCGGATG GACATCTAGA AGATGGCCTT TCAAAGCCGA
     4381 AGGGAGGAGA AGAGGGTTTC TCCACGTTTT TCCATGAAAC CGGCTACGGT AAGTTCGTTC
     4441 CAAGGGCTAT TTACGTGGAT TTAGAGCCCA ACGTTATTGA CGAAGTCCGT AACGGTCCTT
     4501 ACAAGGACTT GTTCCATCCA GAACAGTTGA TCAGTGGTAA GGAGGACGCG GCTAATAATT
     4561 ATGCAAGAGG CCATTACACC GTTGGTAGAG AAATTTTGGG CGATGTTCTG GATAGGATTA
     4621 GAAAACTGGC AGACCAATGT GATGGGTTAC AAGGGTTCTT GTTTACCCAT TCTCTTGGTG
     4681 GTGGTACTGG TTCCGGTCTA GGGTCCTTAC TGTTGGAAGA ACTATCCGCT GAATACGGTA
     4741 AGAAATCCAA GCTGGAATTT GCCGTATACC CTGCTCCACA AGTGTCTACT TCTGTGGTTG
     4801 AGCCTTACAA CACAGTTTTA ACTACACATA CTACATTGGA ACATGCAGAT TGTACTTTCA
     4861 TGGTCGATAA TGAGGCTATT TACGACATGT GCAAAAGAAA CTTGGATATC CCAAGACCAA
     4921 GCTTTGCAAA CTTAAACAAC CTAATTGCTC AAGTGGTATC ATCTGTTACA GCATCATTGA
     4981 GATTCGACGG TTCATTAAAT GTAGATTTGA ACGAATTTCA AACCAATTTG GTTCCATATC
     5041 CAAGAATTCA TTTCCCCTTA GTTTCATATT CTCCAGTCTT ATCCAAATCA AAGGCATTCC
     5101 ATGAGTCCAA CTCTGTGTCA GAAATTACAA ACGCTTGTTT TGAACCTGGT AACCAGATGG
     5161 TCAAGTGTGA TCCAAGAGAT GGTAAATACA TGGCTACTTG TCTGTTATAC AGGGGTGATG
     5221 TGGTAACAAG AGATGTTCAA AGAGCTGTCG AGCAGGTGAA AAACAAGAAG ACCGTCCAAT
     5281 TGGTTGATTG GTGTCCAACT GGTTTCAAGA TCGGTATTTG CTACGAACCT CCAACTGCCA
     5341 CACCAAACTC ACAATTGGCC ACTGTGGATA GGGCCGTCTG TATGTTGTCA AATACCACAT
     5401 CCATTGCTGA GGCTTGGAAG AGAATCGATA GAAAATTCGA TTTAATGTAT GCCAAACGTG
     5461 CTTTCGTCCA CTGGTATGTC GGTGAAGGTA TGGAAGAAGG TGAATTCACC GAAGCTAGAG
     5521 AAGATTTGGC TGCTTTAGAA AGAGATTACA TCGAAGTGGG TGCCGACTCA TACGCTGAGG
     5581 AAGAGGAATT TTAAcgagAT CCtaactcga ggccaattgg tgcggcaatt gataataacg
     5641 aaaatgtctt ttaatgatct gggtataatg aggaattttc cgaacgtttt tactttatat
     5701 atatatatac atgtaacata tattctatac gctatagaga aaggaaattt ttcaattaaa
     5761 aaaaaaatag agaaagagtt tcacttcttg attatcgcta acactaatgg ttgaagtact
     5821 gctactttaa ttttatgctg ccaatgagac gacggggtca tcacggctca tcatgcgcca
     5881 aacaaatgtg tgcaatacac gctcggatga ctgcatgatg accgcactga ctggggacag
     5941 cagatccacc taagcctgtg agagaagcag acacccgaca gatcaaggca gttaactagt
     6001 gcactgcagT ACAaatcgtc cccaacaaaa gtgggctctc aaaattcatc acatttaaat
     6061 gcatatagga agagcaacag ttggtttgca tctgatgttc cttaaagatt tcgacataat
     6121 gtgcgaagta gatCCCTTCA GCACTGTACT TGTTTTTGCG ACTAGATTGT AAATCATTCT
     6181 TTATTTAATC TCTTTCTTTA ACTACTGCTT AAAGTATAAT TTGGTCCGTA GTTTAATAAC
     6241 TATACTAAGC GAACGCTCTC TCGAAATTTT TCTTAACGCG TCCTTGTACT GCGTCTAACG
     6301 CTTTTGCCAC TTGGATTTCT ATTATAGGAA ATAGTCTCAC TTACTGGGCG ACGAATTTTC
     6361 GCGTTTTGAT GAAGCACAGG AAGAATTTCT TTATGctcag aagtttgaca gcaagcaagt
     6421 tcatcattcg aactagcctt attgttttag ttcagtgaca gcgaactgcc gtactcgatg
     6481 ctttatttct cacggtagag cggaagaaca gatagATCCC TACATATTGC TACCACTTCT
     6541 ATTACACAAT AGTTTCAATA GCTTGCAGCG TAGCTAAACT CTAAAATTTA TCTAAATCAC
     6601 TCATATAAAC CGAACCCTTC CCCTTCCGCT TATAGTACAG CTGATGCTCC TTCACTATTT
     6661 TAACATGTGG AATTCTTGAA AGAATGAAAT CGCCATGCCA AGCCATCACA CGGTCTTTTA
     6721 TGCAATTGAT TGACCGCCTG CAACACATAG GCAGTAAAAT TTTTACAAGC AGTAGCAGAA
     6781 CAGGCCACAC AATCGCAAGT GATTAACGTC CACACAGGTA TAGGGTTTCT GGACCATATG
     6841 ATACATGCTC TGGCCAAGCA TTCCGGCTGG TCGCTAATCG TTGAGTGccg agcggccgcg
     6901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6961 ctaaagtata tatgagtaaa cttggtctga cag
//