LOCUS       pBBK88_pHomL-TDH3p-Neon 6525 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(633..652)
                     /label="BB1006. AmpR seq1 R"
                     /note="sequence: taaagttctgctatgtggcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2835..3501
                     /label="TDH3 promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3330..3353
                     /label="BB70. TDH3pr Seq F"
                     /note="sequence: cctgaaattattcccctacttgac"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3476..3501)
                     /label="BB43. TDH3pr R (LN398)"
                     /note="sequence: TTTGTTTGTTTATGTGTGTTTATtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3507..3507
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3508..4215
                     /label="ymNeongreen"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             3508..5109
                     /label="Translation 3508-5109"
     primer          complement(3597..3614)
                     /label="BB1179. mNeon R"
                     /note="sequence: ccttgaccgaccatatcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          3784..3804
                     /label="BB899. NeonGreen seq F"
                     /note="sequence: ggttaccaagtccacagaacc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4216..4238
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            4264..4653
                     /label="PLCd PH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4654..4719
                     /label="PH-PH Link"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4713..4728
                     /label="BB406. PH-Link-PH int Fv2"
                     /note="sequence: GGCAGCTCACGGGCTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            4720..5109
                     /label="PLCd PH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5110..5112
                     /label="Translation 5110-5112"
     misc_feature    5135..5368
                     /label="SSA1t (4)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5322..5344)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5369..5372
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5373..5541
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5542..5545
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5546..5665
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5546..6419
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5644..5664)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5670..5783
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5788..5923
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5895..5915
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5928..6047
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6052..6171
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6149..6168)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6176..6295
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6300..6419
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6420..6423
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gcagttcgag tttatcatta tcaatactgc catttcaaag aatacgtaaa taattaatag
     2881 tagtgatttt cctaacttta tttagtcaaa aaattagcct tttaattctg ctgtaacccg
     2941 tacatgccca aaataggggg cgggttacac agaatatata acatcgtagg tgtctgggtg
     3001 aacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag ctggcatcca
     3061 gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc agttcatagg
     3121 tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa acgggcacaa
     3181 cctcaatgga gtgatgcaac ctgcctggag taaatgatga cacaaggcaa ttgacccacg
     3241 catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct gatttggaaa
     3301 aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt gactaataag
     3361 tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa
     3421 ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaactt agtttcgaat
     3481 aaacacacat aaacaaacaa aagatcTatg gtttctaagg gtgaagaaga taacatggct
     3541 tctttgccag ccacccacga gttgcacatc ttcggttcca ttaacggtgt cgacttcgat
     3601 atggtcggtc aaggtaccgg taacccaaac gacggttacg aagaattgaa cttgaagtct
     3661 accaagggcg acttgcagtt ctccccttgg attttggttc cacacatcgg ttacggtttc
     3721 caccaatact tgccataccc agacggtatg tccccattcc aagccgccat ggttgacggt
     3781 tctggttacc aagtccacag aaccatgcaa ttcgaagatg gtgcttcctt gaccgtgaac
     3841 tacagataca cctacgaagg ttctcacatc aagggcgaag cccaagttaa gggtaccggt
     3901 ttccctgctg acggtccagt catgactaac tccttgaccg ctgctgactg gtgtcgttcc
     3961 aagaaaacct acccaaacga taagaccatc atttccactt tcaagtggtc ctacaccacc
     4021 ggtaacggca agagataccg ctctaccgct cgtaccacct acaccttcgc caagccaatg
     4081 gctgccaact acttgaagaa ccagcctatg tacgttttcc gtaagaccga gctgaagcat
     4141 tccaagactg aattgaactt caaggaatgg caaaaggctt tcaccgacgt catgggtatg
     4201 gacgaattgt acaagTCTGG TCCTTCTGGC CCAACTGGTT CTCCAGGTAG AACTCTCAGA
     4261 TCCCACGGGC TCCAGGATGA CCCGGACCTT CAGGCCCTTC TGAAGGGCAG CCAGCTTCTG
     4321 AAGGTGAAGT CCAGCTCGTG GCGTAGGGAA CGCTTCTACA AGCTACAGGA GGACTGCAAG
     4381 ACCATCTGGC AGGAATCTCG AAAGGTCATG AGGTCCCCGG AGTCGCAGCT GTTCTCCATC
     4441 GAGGACATTC AGGAGGTACG GATGGGACAC CGCACAGAAG GCCTGGAGAA GTTTGCCCGA
     4501 GACATCCCCG AGGATCGATG CTTCTCCATT GTCTTCAAGG ACCAGCGCAA CACCCTAGAC
     4561 CTCATTGCCC CATCACCAGC TGACGCTCAG CACTGGGTGC AGGGCCTGCG CAAGATCATC
     4621 CACCACTCCG GCTCCATGGA CCAGCGGCAG AAGGCAGCGG CaGCAGGtgg cgcATTCaga
     4681 AAGaGCGAAT TCGCCCTTGC GGAATCCGCC GCGGCAGCTC ACGGGCTCCA GGATGACCCG
     4741 GACCTTCAGG CCCTTCTGAA GGGCAGCCAG CTTCTGAAGG TGAAGTCCAG CTCGTGGCGT
     4801 AGGGAACGCT TCTACAAGCT ACAGGAGGAC TGCAAGACCA TCTGGCAGGA ATCTCGAAAG
     4861 GTCATGAGGT CCCCGGAGTC GCAGCTGTTC TCCATCGAGG ACATTCAGGA GGTACGGATG
     4921 GGACACCGCA CAGAAGGCCT GGAGAAGTTT GCCCGAGACA TCCCCGAGGA TCGATGCTTC
     4981 TCCATTGTCT TCAAGGACCA GCGCAACACC CTAGACCTCA TTGCCCCATC ACCAGCTGAC
     5041 GCTCAGCACT GGGTGCAGGG CCTGCGCAAG ATCATCCACC ACTCCGGCTC CATGGACCAG
     5101 CGGCAGAAGT AGTCGACCTC GGTCGCcgag ATCCtaactc gaggccaatt ggtgcggcaa
     5161 ttgataataa cgaaaatgtc ttttaatgat ctgggtataa tgaggaattt tccgaacgtt
     5221 tttactttat atatatatat acatgtaaca tatattctat acgctataga gaaaggaaat
     5281 ttttcaatta aaaaaaaaat agagaaagag tttcacttct tgattatcgc taacactaat
     5341 ggttgaagta ctgctacttt aattttatgc tgccaatgag acgacggggt catcacggct
     5401 catcatgcgc caaacaaatg tgtgcaatac acgctcggat gactgcatga tgaccgcact
     5461 gactggggac agcagatcca cctaagcctg tgagagaagc agacacccga cagatcaagg
     5521 cagttaacta gtgcactgca gTACAaatcg tccccaacaa aagtgggctc tcaaaattca
     5581 tcacatttaa atgcatatag gaagagcaac agttggtttg catctgatgt tccttaaaga
     5641 tttcgacata atgtgcgaag tagatCCCTT CAGCACTGTA CTTGTTTTTG CGACTAGATT
     5701 GTAAATCATT CTTTATTTAA TCTCTTTCTT TAACTACTGC TTAAAGTATA ATTTGGTCCG
     5761 TAGTTTAATA ACTATACTAA GCGAACGCTC TCTCGAAATT TTTCTTAACG CGTCCTTGTA
     5821 CTGCGTCTAA CGCTTTTGCC ACTTGGATTT CTATTATAGG AAATAGTCTC ACTTACTGGG
     5881 CGACGAATTT TCGCGTTTTG ATGAAGCACA GGAAGAATTT CTTTATGctc agaagtttga
     5941 cagcaagcaa gttcatcatt cgaactagcc ttattgtttt agttcagtga cagcgaactg
     6001 ccgtactcga tgctttattt ctcacggtag agcggaagaa cagatagATC CCTACATATT
     6061 GCTACCACTT CTATTACACA ATAGTTTCAA TAGCTTGCAG CGTAGCTAAA CTCTAAAATT
     6121 TATCTAAATC ACTCATATAA ACCGAACCCT TCCCCTTCCG CTTATAGTAC AGCTGATGCT
     6181 CCTTCACTAT TTTAACATGT GGAATTCTTG AAAGAATGAA ATCGCCATGC CAAGCCATCA
     6241 CACGGTCTTT TATGCAATTG ATTGACCGCC TGCAACACAT AGGCAGTAAA ATTTTTACAA
     6301 GCAGTAGCAG AACAGGCCAC ACAATCGCAA GTGATTAACG TCCACACAGG TATAGGGTTT
     6361 CTGGACCATA TGATACATGC TCTGGCCAAG CATTCCGGCT GGTCGCTAAT CGTTGAGTGc
     6421 cgagcggccg cgattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     6481 ttttaaatca atctaaagta tatatgagta aacttggtct gacag
//