LOCUS       pBBK89_pHomL-TDH3p-Scar 6513 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(633..652)
                     /label="BB1006. AmpR seq1 R"
                     /note="sequence: taaagttctgctatgtggcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     promoter        2835..3501
                     /label="TDH3 promoter"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3330..3353
                     /label="BB70. TDH3pr Seq F"
                     /note="sequence: cctgaaattattcccctacttgac"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3476..3501)
                     /label="BB43. TDH3pr R (LN398)"
                     /note="sequence: TTTGTTTGTTTATGTGTGTTTATtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3507..3507
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     ORF             3508..4203
                     /label="ymScarlet"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3508..5097
                     /label="Translation 3508-5097"
     primer          3814..3835
                     /label="BB1186. Scarlet seq F"
                     /note="sequence: ggtggtgctgttactgttactc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4036..4059)
                     /label="BB1187. Scarlet seq R"
                     /note="sequence: agctttataggtggtcttgaagtc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4204..4226
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            4252..4641
                     /label="PLCd PH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4642..4707
                     /label="PH-PH Link"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4701..4716
                     /label="BB406. PH-Link-PH int Fv2"
                     /note="sequence: GGCAGCTCACGGGCTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            4708..5097
                     /label="PLCd PH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5098..5100
                     /label="Translation 5098-5100"
     misc_feature    5123..5356
                     /label="SSA1t (4)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5310..5332)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5357..5360
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5361..5529
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5530..5533
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5534..5653
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5534..6407
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5632..5652)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5658..5771
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5776..5911
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5883..5903
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5916..6035
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6040..6159
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6137..6156)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6164..6283
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6288..6407
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6408..6411
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gcagttcgag tttatcatta tcaatactgc catttcaaag aatacgtaaa taattaatag
     2881 tagtgatttt cctaacttta tttagtcaaa aaattagcct tttaattctg ctgtaacccg
     2941 tacatgccca aaataggggg cgggttacac agaatatata acatcgtagg tgtctgggtg
     3001 aacagtttat tcctggcatc cactaaatat aatggagccc gctttttaag ctggcatcca
     3061 gaaaaaaaaa gaatcccagc accaaaatat tgttttcttc accaaccatc agttcatagg
     3121 tccattctct tagcgcaact acagagaaca ggggcacaaa caggcaaaaa acgggcacaa
     3181 cctcaatgga gtgatgcaac ctgcctggag taaatgatga cacaaggcaa ttgacccacg
     3241 catgtatcta tctcattttc ttacaccttc tattaccttc tgctctctct gatttggaaa
     3301 aagctgaaaa aaaaggttga aaccagttcc ctgaaattat tcccctactt gactaataag
     3361 tatataaaga cggtaggtat tgattgtaat tctgtaaatc tatttcttaa acttcttaaa
     3421 ttctactttt atagttagtc ttttttttag ttttaaaaca ccaagaactt agtttcgaat
     3481 aaacacacat aaacaaacaa aagatcTatg gttagtaaag gtgaagctgt tataaaagaa
     3541 ttcatgaggt ttaaagttca tatggaaggt tcaatgaatg gtcatgaatt tgaaattgaa
     3601 ggtgaaggtg aaggtagacc atatgaaggt acacaaactg ctaaattgaa agttactaaa
     3661 ggtggtccat taccattttc ttgggatatt ttgtctccac aattcatgta tggttctaga
     3721 gcttttatta agcatccagc tgatattcca gattattata aacaatcttt tcctgaaggt
     3781 tttaaatggg aaagagttat gaatttcgaa gatggtggtg ctgttactgt tactcaagat
     3841 acttctttgg aagatggtac tttaatctat aaagttaaat tgagaggtac taattttcca
     3901 ccagatggtc cagttatgca aaagaaaact atgggttggg aagcatctac tgaaagattg
     3961 tatccagaag atggtgtttt gaaaggagac attaagatgg ctttgagatt gaaagatggt
     4021 ggtcggtacc tggccgactt caagaccacc tataaagcta aaaaaccagt tcaaatgcca
     4081 ggtgcatata atgttgatag aaagttagat ataacgtcgc ataacgagga ctatactgta
     4141 gttgaacaat atgaacgtag tgaaggtaga catagtaccg gaggaatgga tgaattgtat
     4201 aaaTCTGGTC CTTCTGGCCC AACTGGTTCT CCAGGTAGAA CTCTCAGATC CCACGGGCTC
     4261 CAGGATGACC CGGACCTTCA GGCCCTTCTG AAGGGCAGCC AGCTTCTGAA GGTGAAGTCC
     4321 AGCTCGTGGC GTAGGGAACG CTTCTACAAG CTACAGGAGG ACTGCAAGAC CATCTGGCAG
     4381 GAATCTCGAA AGGTCATGAG GTCCCCGGAG TCGCAGCTGT TCTCCATCGA GGACATTCAG
     4441 GAGGTACGGA TGGGACACCG CACAGAAGGC CTGGAGAAGT TTGCCCGAGA CATCCCCGAG
     4501 GATCGATGCT TCTCCATTGT CTTCAAGGAC CAGCGCAACA CCCTAGACCT CATTGCCCCA
     4561 TCACCAGCTG ACGCTCAGCA CTGGGTGCAG GGCCTGCGCA AGATCATCCA CCACTCCGGC
     4621 TCCATGGACC AGCGGCAGAA GGCAGCGGCa GCAGGtggcg cATTCagaAA GaGCGAATTC
     4681 GCCCTTGCGG AATCCGCCGC GGCAGCTCAC GGGCTCCAGG ATGACCCGGA CCTTCAGGCC
     4741 CTTCTGAAGG GCAGCCAGCT TCTGAAGGTG AAGTCCAGCT CGTGGCGTAG GGAACGCTTC
     4801 TACAAGCTAC AGGAGGACTG CAAGACCATC TGGCAGGAAT CTCGAAAGGT CATGAGGTCC
     4861 CCGGAGTCGC AGCTGTTCTC CATCGAGGAC ATTCAGGAGG TACGGATGGG ACACCGCACA
     4921 GAAGGCCTGG AGAAGTTTGC CCGAGACATC CCCGAGGATC GATGCTTCTC CATTGTCTTC
     4981 AAGGACCAGC GCAACACCCT AGACCTCATT GCCCCATCAC CAGCTGACGC TCAGCACTGG
     5041 GTGCAGGGCC TGCGCAAGAT CATCCACCAC TCCGGCTCCA TGGACCAGCG GCAGAAGTAG
     5101 TCGACCTCGG TCGCcgagAT CCtaactcga ggccaattgg tgcggcaatt gataataacg
     5161 aaaatgtctt ttaatgatct gggtataatg aggaattttc cgaacgtttt tactttatat
     5221 atatatatac atgtaacata tattctatac gctatagaga aaggaaattt ttcaattaaa
     5281 aaaaaaatag agaaagagtt tcacttcttg attatcgcta acactaatgg ttgaagtact
     5341 gctactttaa ttttatgctg ccaatgagac gacggggtca tcacggctca tcatgcgcca
     5401 aacaaatgtg tgcaatacac gctcggatga ctgcatgatg accgcactga ctggggacag
     5461 cagatccacc taagcctgtg agagaagcag acacccgaca gatcaaggca gttaactagt
     5521 gcactgcagT ACAaatcgtc cccaacaaaa gtgggctctc aaaattcatc acatttaaat
     5581 gcatatagga agagcaacag ttggtttgca tctgatgttc cttaaagatt tcgacataat
     5641 gtgcgaagta gatCCCTTCA GCACTGTACT TGTTTTTGCG ACTAGATTGT AAATCATTCT
     5701 TTATTTAATC TCTTTCTTTA ACTACTGCTT AAAGTATAAT TTGGTCCGTA GTTTAATAAC
     5761 TATACTAAGC GAACGCTCTC TCGAAATTTT TCTTAACGCG TCCTTGTACT GCGTCTAACG
     5821 CTTTTGCCAC TTGGATTTCT ATTATAGGAA ATAGTCTCAC TTACTGGGCG ACGAATTTTC
     5881 GCGTTTTGAT GAAGCACAGG AAGAATTTCT TTATGctcag aagtttgaca gcaagcaagt
     5941 tcatcattcg aactagcctt attgttttag ttcagtgaca gcgaactgcc gtactcgatg
     6001 ctttatttct cacggtagag cggaagaaca gatagATCCC TACATATTGC TACCACTTCT
     6061 ATTACACAAT AGTTTCAATA GCTTGCAGCG TAGCTAAACT CTAAAATTTA TCTAAATCAC
     6121 TCATATAAAC CGAACCCTTC CCCTTCCGCT TATAGTACAG CTGATGCTCC TTCACTATTT
     6181 TAACATGTGG AATTCTTGAA AGAATGAAAT CGCCATGCCA AGCCATCACA CGGTCTTTTA
     6241 TGCAATTGAT TGACCGCCTG CAACACATAG GCAGTAAAAT TTTTACAAGC AGTAGCAGAA
     6301 CAGGCCACAC AATCGCAAGT GATTAACGTC CACACAGGTA TAGGGTTTCT GGACCATATG
     6361 ATACATGCTC TGGCCAAGCA TTCCGGCTGG TCGCTAATCG TTGAGTGccg agcggccgcg
     6421 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     6481 ctaaagtata tatgagtaaa cttggtctga cag
//