LOCUS       pBBK90_pHomL-TEF2p-Scar 6533 bp ds-DNA     circular     04-NOV-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     CDS             complement(1..861)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(90..107)
                     /label="BB871. MoCl vector R"
                     /note="sequence: agccctcccgtatcgtag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(633..652)
                     /label="BB1006. AmpR seq1 R"
                     /note="sequence: taaagttctgctatgtggcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        complement(862..966)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     rep_origin      1069..1657
                     /label="ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     1558..1577
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1593..1610
                     /label="BB774. MoCloEnt chk F"
                     /note="sequence: ttcgccacctctgacttg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          1736..1755
                     /label="BB797. pYES2 chk R"
                     /note="sequence: CCTGATTCTGTGGATAACCG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1765..1768
                     /label="8a>8b"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    1769..1888
                     /label="FF18L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1769..2623
                     /label="HomL (8b)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1893..2012
                     /label="FF21L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2017..2136
                     /label="XII-2L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2050..2072
                     /label="BB777. HomArmL seq F"
                     /note="sequence: CGAGCTAACTATTTTCGACACAC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2144..2263
                     /label="XII-5L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2268..2386
                     /label="ARS308L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2391..2510
                     /label="ARS416L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2515..2623
                     /label="HIS3L"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2627
                     /label="8b>1"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2628..2817
                     /label="ConLS (1)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          2724..2743
                     /label="BB867. ConLS F"
                     /note="sequence: gcgatcacagacattaaccc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2818..2821
                     /label="1>2"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2822..3526
                     /label="TEF2p (2)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3337..3357
                     /label="BB868. TEF2p F"
                     /note="sequence: agtgctatttctggcttttcc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3527..3527
                     /label="2>3"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     ORF             3528..4223
                     /label="ymScarlet"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     CDS             3528..5117
                     /label="Translation 3528-5117"
     primer          3834..3855
                     /label="BB1186. Scarlet seq F"
                     /note="sequence: ggtggtgctgttactgttactc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(4056..4079)
                     /label="BB1187. Scarlet seq R"
                     /note="sequence: agctttataggtggtcttgaagtc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    4224..4246
                     /label="Link"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     gene            4272..4661
                     /label="PLCd PH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    4662..4727
                     /label="PH-PH Link"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4721..4736
                     /label="BB406. PH-Link-PH int Fv2"
                     /note="sequence: GGCAGCTCACGGGCTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            4728..5117
                     /label="PLCd PH"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5118..5120
                     /label="Translation 5118-5120"
     misc_feature    5143..5376
                     /label="SSA1t (4)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5330..5352)
                     /label="BB502. SSA1 Chk R"
                     /note="sequence: AACCATTAGTGTTAGCGATAATC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5377..5380
                     /label="4>5"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5381..5549
                     /label="ConR1 (5)"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    5550..5553
                     /label="5>(6/7)"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    5554..5673
                     /label="FF18R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5554..6427
                     /label="HomArmR (6/7)"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5652..5672)
                     /label="BB239. FF18d seq R"
                     /note="sequence: tctacttcgcacattatgtcg"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5678..5791
                     /label="FF21R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    5796..5931
                     /label="XII-2R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          5903..5923
                     /label="BB778. HomArmR seq F"
                     /note="sequence: GTTTTGATGAAGCACAGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    5936..6055
                     /label="XII-5R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6060..6179
                     /label="ARS308R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6157..6176)
                     /label="BB870. ARS308r R"
                     /note="sequence: ACTATAAGCGGAAGGGGAAG"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    6184..6303
                     /label="ARS416R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6308..6427
                     /label="HIS3R"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6428..6431
                     /label="7>8a"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
ORIGIN
        1 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
       61 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
      121 cagtgctgca atgataccgc gggacccacg ctcaccggct ccagatttat cagcaataaa
      181 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
      241 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
      301 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
      361 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
      421 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
      481 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
      541 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
      601 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
      661 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
      721 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
      781 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
      841 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
      901 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
      961 tccgcgcaca tttccccgaa aagtgccacc tgtcatgacc aaaatccctt aacgtgagtt
     1021 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     1081 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     1141 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     1201 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     1261 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     1321 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     1381 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     1441 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     1501 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     1561 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     1621 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     1681 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     1741 ttctgtggat aaccgtgcgg ccgcCAATgt tatctattgg cagatgttca tacggggctt
     1801 tcaaatattg atgaaattat gtgatgttta gaagaagatt cgaactgttt tcagtagatt
     1861 tggtaactgt gcaaccataa ctcatgccCC CTATTTATCG GCATTGAGAT TGTAAAGGAA
     1921 GTAAAACGCA TTTTTGCAGA TCTGTTCTCT TATGTATTTT TAATCGTCCT TGTATGGAAG
     1981 TATCAAAGGG GACGTTCTTC ACCTCCTTGG AAAACGCAAA CTTTCTTTTT GCCTATCACA
     2041 GTATCTTATC GAGCTAACTA TTTTCGACAC ACATGAAAAA GCAGAAATAT TAACGAAAAA
     2101 GAAAAGAAAG ACCATGTCAT GTACGGGCAA TCAGAATATG caagatgagc gggaacgggc
     2161 gaaatgtgca cgcgcacaac ttacgaaacg cggatgagtc actgacagcc accgcagagg
     2221 ttctgactcc tactgagctc tattggaggt ggcagaaccg gtaATCCCAC AGTGAGCAAC
     2281 TTGACAAACC AACAGATATA GGCTGTGTCT TAAAATATAC CATCCAATAC CTTGATGAAC
     2341 TTTTCAAAGA TGACTAAAAT AAGTGAAATT TCAACATTAA CTTCGAGCTG TAAACACAGT
     2401 GGTTTCTTTG CATAAACACC ATCAGCCTCA AGTCGTCAAG TAAAGATTTC GTGTTCATGC
     2461 AGATAGATAA CAATCTATAT GTTGATAATT AGCGTTGCCT CATCAATGCG TACACGAAGA
     2521 ATATACTAAA AAATGAGCAG GCAAGATAAA CGAAGGCAAA GATGACAGAG CAGAAAGCCC
     2581 TAGTAAAGCG TATTACAAAT GAAACCAAGA TTCAGATTGC GATccctgaa ttcgcatcta
     2641 gatggtagag ccacaaacag ccggtacaag caacgatctc caggaccatc tgaatcatgc
     2701 gcggatgaca cgaactcacg acggcgatca cagacattaa cccacagtac agacactgcg
     2761 acaacgtggc aattcgtcgc aataccgtct cactgaactg gccgataatt gcagacgaac
     2821 gttgataggt caagatcaat gtaaacaatt actttgttat gtagagtttt tttagctacc
     2881 tatattccac cataacatca atcatgcggt tgctggtgta tttaccaata atgtttaatg
     2941 tatatatata tatatatata tggggccgta tacttacata tagtagatgt caagcgtagg
     3001 cgcttcccct gccggctgtg agggcgccat aaccaaggta tctatagacc gccaatcagc
     3061 aaactacctc cgtacattca tgttgcaccc acacatttat acacccagac cgcgacaaat
     3121 tacccataag gttgtttgtg acggcgtcgt acaagagaac gtgggaactt tttaggctca
     3181 ccaaaaaaga aagaaaaaat acgagttgct gacagaagcc tcaagaaaaa aaaaattctt
     3241 cttcgactat gctggaggca gagatgatcg agccggtagt taactatata tagctaaatt
     3301 ggttccatca ccttcttttc tggtgtcgct ccttctagtg ctatttctgg cttttcctat
     3361 tttttttttt ccatttttct ttctctcttt ctaatatata aattctcttg cattttctat
     3421 ttttctctct atctattcta cttgtttatt cccttcaagg ttttttttta aggagtactt
     3481 gtttttagaa tatacggtca acgaactata attaactaaa cagatcTatg gttagtaaag
     3541 gtgaagctgt tataaaagaa ttcatgaggt ttaaagttca tatggaaggt tcaatgaatg
     3601 gtcatgaatt tgaaattgaa ggtgaaggtg aaggtagacc atatgaaggt acacaaactg
     3661 ctaaattgaa agttactaaa ggtggtccat taccattttc ttgggatatt ttgtctccac
     3721 aattcatgta tggttctaga gcttttatta agcatccagc tgatattcca gattattata
     3781 aacaatcttt tcctgaaggt tttaaatggg aaagagttat gaatttcgaa gatggtggtg
     3841 ctgttactgt tactcaagat acttctttgg aagatggtac tttaatctat aaagttaaat
     3901 tgagaggtac taattttcca ccagatggtc cagttatgca aaagaaaact atgggttggg
     3961 aagcatctac tgaaagattg tatccagaag atggtgtttt gaaaggagac attaagatgg
     4021 ctttgagatt gaaagatggt ggtcggtacc tggccgactt caagaccacc tataaagcta
     4081 aaaaaccagt tcaaatgcca ggtgcatata atgttgatag aaagttagat ataacgtcgc
     4141 ataacgagga ctatactgta gttgaacaat atgaacgtag tgaaggtaga catagtaccg
     4201 gaggaatgga tgaattgtat aaaTCTGGTC CTTCTGGCCC AACTGGTTCT CCAGGTAGAA
     4261 CTCTCAGATC CCACGGGCTC CAGGATGACC CGGACCTTCA GGCCCTTCTG AAGGGCAGCC
     4321 AGCTTCTGAA GGTGAAGTCC AGCTCGTGGC GTAGGGAACG CTTCTACAAG CTACAGGAGG
     4381 ACTGCAAGAC CATCTGGCAG GAATCTCGAA AGGTCATGAG GTCCCCGGAG TCGCAGCTGT
     4441 TCTCCATCGA GGACATTCAG GAGGTACGGA TGGGACACCG CACAGAAGGC CTGGAGAAGT
     4501 TTGCCCGAGA CATCCCCGAG GATCGATGCT TCTCCATTGT CTTCAAGGAC CAGCGCAACA
     4561 CCCTAGACCT CATTGCCCCA TCACCAGCTG ACGCTCAGCA CTGGGTGCAG GGCCTGCGCA
     4621 AGATCATCCA CCACTCCGGC TCCATGGACC AGCGGCAGAA GGCAGCGGCa GCAGGtggcg
     4681 cATTCagaAA GaGCGAATTC GCCCTTGCGG AATCCGCCGC GGCAGCTCAC GGGCTCCAGG
     4741 ATGACCCGGA CCTTCAGGCC CTTCTGAAGG GCAGCCAGCT TCTGAAGGTG AAGTCCAGCT
     4801 CGTGGCGTAG GGAACGCTTC TACAAGCTAC AGGAGGACTG CAAGACCATC TGGCAGGAAT
     4861 CTCGAAAGGT CATGAGGTCC CCGGAGTCGC AGCTGTTCTC CATCGAGGAC ATTCAGGAGG
     4921 TACGGATGGG ACACCGCACA GAAGGCCTGG AGAAGTTTGC CCGAGACATC CCCGAGGATC
     4981 GATGCTTCTC CATTGTCTTC AAGGACCAGC GCAACACCCT AGACCTCATT GCCCCATCAC
     5041 CAGCTGACGC TCAGCACTGG GTGCAGGGCC TGCGCAAGAT CATCCACCAC TCCGGCTCCA
     5101 TGGACCAGCG GCAGAAGTAG TCGACCTCGG TCGCcgagAT CCtaactcga ggccaattgg
     5161 tgcggcaatt gataataacg aaaatgtctt ttaatgatct gggtataatg aggaattttc
     5221 cgaacgtttt tactttatat atatatatac atgtaacata tattctatac gctatagaga
     5281 aaggaaattt ttcaattaaa aaaaaaatag agaaagagtt tcacttcttg attatcgcta
     5341 acactaatgg ttgaagtact gctactttaa ttttatgctg ccaatgagac gacggggtca
     5401 tcacggctca tcatgcgcca aacaaatgtg tgcaatacac gctcggatga ctgcatgatg
     5461 accgcactga ctggggacag cagatccacc taagcctgtg agagaagcag acacccgaca
     5521 gatcaaggca gttaactagt gcactgcagT ACAaatcgtc cccaacaaaa gtgggctctc
     5581 aaaattcatc acatttaaat gcatatagga agagcaacag ttggtttgca tctgatgttc
     5641 cttaaagatt tcgacataat gtgcgaagta gatCCCTTCA GCACTGTACT TGTTTTTGCG
     5701 ACTAGATTGT AAATCATTCT TTATTTAATC TCTTTCTTTA ACTACTGCTT AAAGTATAAT
     5761 TTGGTCCGTA GTTTAATAAC TATACTAAGC GAACGCTCTC TCGAAATTTT TCTTAACGCG
     5821 TCCTTGTACT GCGTCTAACG CTTTTGCCAC TTGGATTTCT ATTATAGGAA ATAGTCTCAC
     5881 TTACTGGGCG ACGAATTTTC GCGTTTTGAT GAAGCACAGG AAGAATTTCT TTATGctcag
     5941 aagtttgaca gcaagcaagt tcatcattcg aactagcctt attgttttag ttcagtgaca
     6001 gcgaactgcc gtactcgatg ctttatttct cacggtagag cggaagaaca gatagATCCC
     6061 TACATATTGC TACCACTTCT ATTACACAAT AGTTTCAATA GCTTGCAGCG TAGCTAAACT
     6121 CTAAAATTTA TCTAAATCAC TCATATAAAC CGAACCCTTC CCCTTCCGCT TATAGTACAG
     6181 CTGATGCTCC TTCACTATTT TAACATGTGG AATTCTTGAA AGAATGAAAT CGCCATGCCA
     6241 AGCCATCACA CGGTCTTTTA TGCAATTGAT TGACCGCCTG CAACACATAG GCAGTAAAAT
     6301 TTTTACAAGC AGTAGCAGAA CAGGCCACAC AATCGCAAGT GATTAACGTC CACACAGGTA
     6361 TAGGGTTTCT GGACCATATG ATACATGCTC TGGCCAAGCA TTCCGGCTGG TCGCTAATCG
     6421 TTGAGTGccg agcggccgcg attatcaaaa aggatcttca cctagatcct tttaaattaa
     6481 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cag
//