LOCUS       Exported               13663 bp DNA     circular SYN 09-OCT-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Oct 27, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13663
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        2..200
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             218..398
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     LTR             220..321
                     /label=R
                     /note="R"
     LTR             322..399
                     /label=U5
                     /note="U5"
     misc_feature    445..570
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    450..587
                     /label=psi
                     /note="psi"
     misc_feature    554..918
                     /label=gag
                     /note="gag"
     misc_feature    1063..1296
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1823..1940
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    complement(1852..1876)
                     /label=Kermit
     promoter        1991..2231
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2217..2233
                     /label=Neon
     misc_feature    2220..2240
                     /label=Argon
     misc_feature    2241..2260
                     /label=BsmBIx2-6T
     misc_RNA        2261..2336
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2337..2358
                     /label=10x GuideCapture 2
     misc_feature    complement(2378..2402)
                     /label=Beaker
     promoter        2420..2631
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     2573..2592
                     /label=o1309
     primer_bind     2594..2613
                     /label=o1307
     primer_bind     2595..2614
                     /label=Primer 1
     primer_bind     2595..2614
                     /label=o1305
     CDS             2645..2680
                     /codon_start=1
                     /label=Start
                     /translation="MKRTADGSEFES"
     CDS             2681..2701
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    2702..3199
                     /label=ABE8e
     primer_bind     complement(2885..2897)
                     /label=o1313
     CDS             3200..3295
                     /codon_start=1
                     /label=Linker_in_RDA_077
                     /translation="SGGSSGGSSGSETPGTSESATPESSGGSSGGS"
     CDS             3224..3271
                     /codon_start=1
                     /label=XTEN linker
                     /translation="SGSETPGTSESATPES"
     CDS             3296..7396
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(3300..3319)
                     /label=Primer 2
     primer_bind     complement(3300..3319)
                     /label=o1306
     misc_feature    3320..3322
                     /label=GAC_to_GCC(SeqWell)
                     /note="GPP expects for GAC, but SeqWell shows GCC"
     primer_bind     complement(3362..3381)
                     /label=o1310
     primer_bind     complement(3424..3443)
                     /label=o1308
     primer_bind     7354..7373
                     /label=o1311
     primer_bind     7365..7384
                     /label=o1313
     CDS             7439..7459
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7469..7525
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(7514..7533)
                     /label=o1312
     CDS             7526..8122
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7660..7679)
                     /label=o1314
     misc_feature    8138..8726
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    complement(8204..8227)
                     /label=Gonzo
     CDS             complement(8609..8620)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8798..9031
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     LTR             8853..8954
                     /label=R
                     /note="R"
     LTR             8955..9032
                     /label=U5
                     /note="U5"
     polyA_signal    9063..9287
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9333..9761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9775..10104
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9955..10090
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10218..10592
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10722..10843
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(11294..11882)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(12053..12913)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(12914..13018)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        13284..13663
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat
      241 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
      301 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
      361 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
      421 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
      481 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
      541 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
      601 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
      661 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
      721 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
      781 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
      841 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
      901 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
      961 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1021 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1081 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1141 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1201 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1261 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1321 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1381 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1441 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1501 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1561 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1621 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1681 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1741 cagagacaga tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac
     1801 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     1861 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     1921 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa
     1981 ttaaggtacc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     2041 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     2101 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     2161 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     2221 gtggaaagga cgaaacaccg gagacgcttt tttcgtctca gtttgagagc tagaaatagc
     2281 aagttcaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgcgctc
     2341 acctattagc ggctaaggtt ttttgaattc gctagctagg tcttgaaagg agtgggaatt
     2401 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     2461 ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     2521 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     2581 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggtgc
     2641 caccatgaaa cggacagccg acggaagcga gttcgagtca ccaaagaaga agcggaaagt
     2701 ctctgaggtg gagttttccc acgagtactg gatgagacat gccctgaccc tggccaagag
     2761 ggcacgggat gagagggagg tgcctgtggg agccgtgctg gtgctgaaca atagagtgat
     2821 cggcgagggc tggaacagag ccatcggcct gcacgaccca acagcccatg ccgaaattat
     2881 ggccctgaga cagggcggcc tggtcatgca gaactacaga ctgattgacg ccaccctgta
     2941 cgtgacattc gagccttgcg tgatgtgcgc cggcgccatg atccactcta ggatcggccg
     3001 cgtggtgttt ggatggagaa attctaaaag aggcgccgca ggctccctga tgaacgtgct
     3061 gaactacccc ggcatgaatc accgcgtcga aattaccgag ggaatcctgg cagatgaatg
     3121 tgccgccctg ctgtgcgatt tctatcggat gcctagacag gtgttcaatg ctcagaagaa
     3181 ggcccagagc tccatcaact ctggaggatc tagcggagga tcctctggca gcgagacacc
     3241 aggaacaagc gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa
     3301 gaagtacagc atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga
     3361 cgagtacaag gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat
     3421 caagaagaac ctgatcggag ccctgctgtt cgacagcggc gaaacagccg aggccacccg
     3481 gctgaagaga accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca
     3541 agagatcttc agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga
     3601 gtccttcctg gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt
     3661 ggacgaggtg gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt
     3721 ggacagcacc gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa
     3781 gttccggggc cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa
     3841 gctgttcatc cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc
     3901 cagcggcgtg gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga
     3961 aaatctgatc gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc
     4021 cctgagcctg ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa
     4081 actgcagctg agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg
     4141 cgaccagtac gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag
     4201 cgacatcctg agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa
     4261 gagatacgac gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct
     4321 gcctgagaag tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat
     4381 tgacggcgga gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat
     4441 ggacggcacc gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg
     4501 gaccttcgac aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct
     4561 gcggcggcag gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat
     4621 cctgaccttc cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc
     4681 ctggatgacc agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga
     4741 caagggcgct tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc
     4801 caacgagaag gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga
     4861 gctgaccaaa gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga
     4921 gcagaaaaag gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca
     4981 gctgaaagag gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt
     5041 ggaagatcgg ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga
     5101 caaggacttc ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct
     5161 gacactgttt gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt
     5221 cgacgacaaa gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag
     5281 ccggaagctg atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct
     5341 gaagtccgac ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac
     5401 ctttaaagag gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca
     5461 cattgccaat ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt
     5521 ggtggacgag ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat
     5581 ggccagagag aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg
     5641 gatcgaagag ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa
     5701 cacccagctg cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta
     5761 cgtggaccag gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc
     5821 tcagagcttt ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa
     5881 ccggggcaag agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg
     5941 gcggcagctg ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc
     6001 cgagagaggc ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga
     6061 aacccggcag atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta
     6121 cgacgagaat gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt
     6181 gtccgatttc cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca
     6241 cgcccacgac gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa
     6301 gctggaaagc gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc
     6361 caagagcgag caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat
     6421 gaactttttc aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat
     6481 cgagacaaac ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt
     6541 gcggaaagtg ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg
     6601 cggcttcagc aaagagtcta tcctgcccaa gaggaacagc gataagctga tcgccagaaa
     6661 gaaggactgg gaccctaaga agtacggcgg cttcgacagc cccaccgtgg cctattctgt
     6721 gctggtggtg gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct
     6781 gctggggatc accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga
     6841 agccaagggc tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct
     6901 gttcgagctg gaaaacggcc ggaagagaat gctggcctct gccggcgagc tgcagaaggg
     6961 aaacgaactg gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga
     7021 gaagctgaag ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa
     7081 gcactacctg gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc
     7141 cgacgctaat ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag
     7201 agagcaggcc gagaatatca tccacctgtt taccctgacc aatctgggag cccctgccgc
     7261 cttcaagtac tttgacacca ccatcgaccg gaagaggtac accagcacca aagaggtgct
     7321 ggacgccacc ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc
     7381 tcagctggga ggtgacagcg gaggctccaa aagaaccgcc gacggcagcg agttcgagcc
     7441 caagaagaag aggaaagtca gcgctggcgc aacaaacttc tctctgctga aacaagccgg
     7501 agatgtcgaa gagaatcctg gaccgaccga gtacaagccc acggtgcgcc tcgccacccg
     7561 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     7621 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     7681 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     7741 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     7801 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     7861 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggag tctcgcccga
     7921 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     7981 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     8041 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     8101 gacccgcaag cccggtgcct gaacgcgtta agtcgacaat caacctctgg attacaaaat
     8161 ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc
     8221 tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt
     8281 gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg
     8341 cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg
     8401 tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc
     8461 cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt
     8521 gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct
     8581 gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg
     8641 cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg
     8701 gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct
     8761 gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa
     8821 cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc
     8881 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     8941 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     9001 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     9061 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     9121 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     9181 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     9241 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     9301 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     9361 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     9421 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     9481 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     9541 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     9601 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     9661 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     9721 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     9781 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9841 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9901 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9961 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    10021 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
    10081 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
    10141 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
    10201 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
    10261 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
    10321 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
    10381 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
    10441 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
    10501 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
    10561 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
    10621 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
    10681 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
    10741 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10801 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
    10861 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
    10921 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
    10981 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
    11041 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
    11101 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    11161 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    11221 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    11281 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    11341 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    11401 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    11461 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    11521 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    11581 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    11641 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    11701 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
    11761 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    11821 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    11881 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    11941 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
    12001 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
    12061 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
    12121 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
    12181 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
    12241 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
    12301 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
    12361 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
    12421 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
    12481 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
    12541 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
    12601 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
    12661 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
    12721 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
    12781 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
    12841 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
    12901 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
    12961 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    13021 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
    13081 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
    13141 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
    13201 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
    13261 tgtacgggcc agatatacgc gttgacattg attattgact agttattaat agtaatcaat
    13321 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
    13381 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
    13441 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
    13501 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
    13561 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
    13621 tacttggcag tacatctacg tattagtcat cgctattacc atg
//
