LOCUS       Exported                9519 bp ds-DNA     circular SYN 16-NOV-2021
DEFINITION  Lentiviral vector expressing N-terminally tagged 
            eGFP-NDP52/CALCOCO2.
ACCESSION   .
VERSION     .
KEYWORDS    pHAGE-eGFP-NDP52
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Heo JM, Ordureau A, Paulo JA, Rinehart J, Harper JW
  TITLE     The PINK1-PARKIN Mitochondrial Ubiquitylation Pathway Drives a 
            Program of OPTN/NDP52 Recruitment and TBK1 Activation to Promote 
            Mitophagy.
  JOURNAL   Mol Cell. 2015 Oct 1;60(1):7-20. doi: 10.1016/j.molcel.2015.08.016. 
            Epub 2015 Sep 10.
  PUBMED    26365381
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Nov 16, 2021 from SnapGene 5.1.7
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Oct 1"; volume: "60(1)"; pages: "7-20. doi: 
            10.1016/j.molcel.2015.08.016. Epub 2015 Sep 10"
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2685..2705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2731..2755
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2814..2823
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2820..3536
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             2820..3536
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2865..2886)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3126..3145)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3473..3494
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    3552..3575
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3576..4754
                     /label=NAP1
     protein_bind    complement(4756..4780)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4799..5298
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4834..4857)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5231..5250
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5322..5720
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    5762..5883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5799..5818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    5909..6497
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5962..5982)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6380..6391)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6572..6805
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(6924..6942)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7010..7114
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7115..7975
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7333..7352)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8146..8734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8635..8654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8888..8905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     8997..9016
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(9020..9031)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    9145..9166
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9181..9211
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9219..9235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9224..9246
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9243..9259
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9243..9259
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccga tctgccacca
     2821 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     2881 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     2941 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     3001 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     3061 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     3121 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     3181 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     3241 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     3301 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     3361 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     3421 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     3481 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaaggctc
     3541 gaggttatca aacaagtttg tacaaaaaag ttggcatgga tgcactggta gaagatgata
     3601 tctgtattct gaatcatgaa aaagcccata agagagatac agtgactcca gtttcaatat
     3661 attcaggaga tgaatctgtt gcttcccatt ttgctcttgt cactgcatat gaagacatca
     3721 aaaaacgact taaggattca gagaaagaga actctttgtt aaagaagaga ataagatttt
     3781 tggaagaaaa gctaatagct cgatttgaag aagaaacaag ttccgtggga cgagaacaag
     3841 taaataaggc ctatcatgca tatcgagagg tttgcattga tagagataat ttgaagagca
     3901 aactggacaa aatgaataaa gacaactctg aatctttgaa agtattgaat gagcagctac
     3961 aatctaaaga agtagaactc ctccagctga ggacagaggt ggaaactcag caggtgatga
     4021 ggaatttaaa tccaccttca tcaaactggg aggtggaaaa gttgagctgt gacctgaaga
     4081 tccatggttt ggaacaagag ctggaactga tgaggaaaga atgtagcgat ctcaaaatag
     4141 aactacagaa agccaaacaa acggatccat atcaggaaga caatctgaag agcagagatc
     4201 tccaaaaact aagcatttca agtgataata tgcagcatgc atactgggaa ctgaagagag
     4261 aaatgtctaa tttacatctg gtgactcaag tacaagctga actactaaga aaactgaaaa
     4321 cctcaactgc aatcaagaaa gcctgtgccc ctgtaggatg cagtgaagac cttggaagag
     4381 acagcacaaa actgcacttg atgaatttta ctgcaacata cacaagacat ccccctctct
     4441 taccaaatgg caaagctctt tgtcatacca catcttcccc tttaccagga gatgtaaagg
     4501 ttttatcaga gaaagcaatc ctccaatcat ggacagacaa tgagagatcc attcctaatg
     4561 atggtacatg ctttcaggaa cacagttctt atggcagaaa ttctctggaa gacaattcct
     4621 gggtatttcc aagtcctcct aaatcaagtg agacagcatt tggggaaact aaaactaaaa
     4681 ctttgccttt acccaacctt ccaccactgc attacttgga tcaacataat cagaactgcc
     4741 tttataagaa ttaatgccca actttcttgt acaaagtggt tcgataacga attctaccgg
     4801 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     4861 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     4921 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     4981 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     5041 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5101 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5161 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5221 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5281 tcatctccgg gcctttcgac ctgcagccca ttaattaaac catggccaag cctttgtctc
     5341 aagaagaatc caccctcatt gaaagagcaa cggctacaat caacagcatc cccatctctg
     5401 aagactacag cgtcgccagc gcagctctct ctagcgacgg ccgcatcttc actggtgtca
     5461 atgtatatca ttttactggg ggaccttgtg cagaactcgt ggtgctgggc actgctgctg
     5521 ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg aaatgagaac aggggcatct
     5581 tgagcccctg cggacggtgc cgacaggtgc ttctcgatct gcatcctggg atcaaagcca
     5641 tagtgaagga cagtgatgga cagccgacgg cagttgggat tcgtgaattg ctgccctctg
     5701 gttatgtgtg ggagggctaa gcacttcgtg gccgaggagc aggactgaca ctcgacctcg
     5761 aaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5821 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5881 ttatcatgtc tatgcatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag
     5941 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6001 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6061 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     6121 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     6181 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     6241 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     6301 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     6361 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     6421 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     6481 ttgggccgcc tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat
     6541 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     6601 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     6661 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6721 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6781 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     6841 taacttgcaa agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct
     6901 agatcattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6961 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7021 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     7081 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     7141 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     7201 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     7261 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     7321 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     7381 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     7441 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     7501 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     7561 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     7621 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     7681 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     7741 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     7801 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     7861 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     7921 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     7981 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     8041 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     8101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8281 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8761 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8821 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8881 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     8941 ccccgcgcgt tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt
     9001 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     9061 tttggaggcc taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa
     9121 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
     9181 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
     9241 cacaggaaac agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca
     9301 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat
     9361 aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa
     9421 ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag
     9481 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//
