LOCUS       pDY0663_New_Scaffold_AC 2377 bp ds-DNA     circular     10-AUG-2021
DEFINITION  .
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     rep_origin      complement(38..626)
                     /label="ori"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer_bind     complement(118..137)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             complement(797..1657)
                     /label="AmpR"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     1420..1439
                     /label="Amp-R"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(1658..1762)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    1811..1838
                     /label="PD4074"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1812..1840
                     /label="PD3842"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    1814..1842
                     /label="PD3749"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     source          join(1869..2377,1..1868)
                     /label="source"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     1869..1889
                     /label="hU6-F"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        1869..2109
                     /label="U6 promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     2040..2059
                     /label="LKO.1 5'"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(2116..2137)
                     /label="PD4075"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2117..2137)
                     /label="PD3750"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2118..2137
                     /label="ACTB guide"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2138..2223
                     /label="Optimized scaffold"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2224..2252
                     /label="RT template"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2253..2298
                     /label="Bxb1 site"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2275..2276
                     /label="di nuc"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2299..2311
                     /label="PBS"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
       61 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      121 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      181 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      241 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      301 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      361 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      421 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      481 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
      541 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
      601 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
      661 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
      721 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
      781 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
      841 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
      901 gcttaccatc tggccccagt gctgcaatga taccgcgaga tccacgctca ccggctccag
      961 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     1021 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     1081 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     1141 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     1201 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     1261 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     1321 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     1381 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     1441 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     1501 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     1561 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     1621 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     1681 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     1741 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgctagct
     1801 gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac tggatccggt accaaggtcg
     1861 ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg
     1921 ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta caaaatacgt
     1981 gacgtagaaa gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg
     2041 actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2101 ggaaaggacg aaacaccGCT ATTCTCGCAG CTCACCAGTT TgAGAGCTAt gctgGAAAca
     2161 gcaTAGCAAG TTcAAATAAG GCTAGTCCGT TATCAACTTG AAAAAGTGGC ACCGAGTCGG
     2221 TGCGACGAGC GCGGCGATAT CATCATCCAT GGccggATGA TCCTGACGAC GGagaccgcc
     2281 gtcgtcgaca agccggccTG AGCTGCGAGA Atttttttaa gcttgggccg ctcgaggtac
     2341 ctctctacat atgacatgtg agcaaaaggc cagcaaa
//