LOCUS       pDY0310_pCAG-TP901-1_In 6317 bp ds-DNA     circular     02-SEP-2021
DEFINITION  .
KEYWORDS    "accession:addgene_51271_256264"                                   
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..6317
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     enhancer        6..385
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        387..664
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     intron          665..1681
                     /label="chimeric intron"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     1605..1627
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1684..1696
                     /label="F common for Bxb1 mutants"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     1694..1696
                     /label="T7"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        1694..1696
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    1697..3252
                     /label="TP901-1 Integrase"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1729..3216
                     /label="Translation 1729-3216"
     primer_bind     complement(3253..3253)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    3253..3466
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3538..3554)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(3538..3555)
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(3547..3569)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      3696..4124
                     /label="f1 ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(3783..3802)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     3993..4014
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        4177..4281
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4282..5142
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(4500..4519)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      5313..5901
                     /label="ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     5802..5821
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6055..6072
                     /label="L4440"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    6189..6210
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        6225..6255
                     /label="lac promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    6263..6279
                     /label="lac operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     6268..6290
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6287..6303
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     6287..6303
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccAT GACAGCGCCA
     1741 AAGAAAAAGA GGAAGGTCAT GACCAAGAAA GTGGCCATCT ATACTAGAGT GAGCACAACG
     1801 AATCAGGCCG AGGAGGGGTT CTCTATTGAC GAGCAAATCG ATCGTCTGAC CAAGTACGCG
     1861 GAAGCAATGG GCTGGCAAGT CAGCGACACT TACACCGATG CTGGGTTCTC CGGCGCCAAA
     1921 CTGGAAAGGC CTGCCATGCA GCGGCTGATT AACGACATTG AGAACAAGGC CTTTGATACA
     1981 GTGCTCGTAT ACAAGCTCGA CAGGCTCAGC CGATCTGTGC GGGACACGCT TTACCTCGTA
     2041 AAGGATGTTT TCACTAAGAA TAAAATCGAC TTCATTAGCC TGAACGAATC CATTGACACC
     2101 AGCTCAGCTA TGGGCTCTCT GTTCCTGACC ATCCTGAGCG CTATCAATGA GTTTGAGAGG
     2161 GAGAATATAA AGGAGCGCAT GACAATGGGA AAGCTGGGTA GAGCGAAGTC CGGGAAATCT
     2221 ATGATGTGGA CCAAGACCGC TTTTGGATAC TACCACAATA GGAAGACGGG CATTCTGGAG
     2281 ATCGTGCCCT TGCAGGCAAC CATCGTTGAG CAGATCTTCA CCGACTACCT GAGCGGAATA
     2341 TCTCTCACGA AGTTGCGAGA TAAGCTGAAT GAGAGCGGAC ACATTGGCAA GGATATTCCT
     2401 TGGTCATATA GAACCCTCCG CCAAACTCTG GATAATCCGG TGTACTGCGG TTACATCAAG
     2461 TTCAAAGACA GCCTCTTCGA GGGAATGCAT AAACCTATCA TTCCATACGA GACATACCTG
     2521 AAAGTCCAAA AGGAACTCGA AGAGCGCCAG CAACAGACTT ACGAACGGAA TAATAATCCC
     2581 AGGCCTTTCC AGGCCAAATA TATGCTGTCC GGCATGGCAA GATGCGGATA CTGCGGGGCA
     2641 CCACTCAAGA TTGTGCTTGG CCATAAACGG AAGGATGGAA GCAGAACCAT GAAATATCAC
     2701 TGCGCAAACC GCTTTCCAAG GAAAACGAAG GGGATTACCG TGTACAATGA CAACAAAAAA
     2761 TGTGATAGCG GAACCTACGA TCTGTCCAAC TTGGAAAACA CCGTCATTGA CAATTTAATT
     2821 GGATTTCAGG AAAATAATGA CAGCCTTCTG AAGATTATCA ACGGGAACAA TCAGCCGATT
     2881 CTGGACACTT CATCTTTCAA AAAACAGATC TCTCAGATTG ATAAGAAAAT TCAGAAAAAT
     2941 TCCGATTTAT ACCTCAATGA TTTCATAACG ATGGATGAGC TGAAGGACCG GACCGACAGT
     3001 TTGCAGGCCG AGAAGAAACT GCTGAAAGCA AAGATCTCCG AGAACAAGTT CAATGACAGT
     3061 ACCGATGTCT TCGAGTTGGT GAAGACCCAG CTGGGTAGTA TCCCAATCAA CGAGTTGAGC
     3121 TATGACAATA AGAAGAAGAT TGTTAATAAC CTGGTGAGCA AAGTGGACGT GACCGCTGAT
     3181 AACGTGGATA TTATCTTCAA GTTCCAGCTG GCCTGAgtca gagctcgctg atcagcctcg
     3241 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     3301 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     3361 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     3421 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     3481 agaaccagct ggggctcgag atccactagt tctagcctcg aggctagagc ggccgccact
     3541 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3601 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3661 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     3721 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     3781 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     3841 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     3901 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     3961 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4021 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4081 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     4141 gcttacrmkt ymsrtkssmc wttymggsga aatgtgcgcg gaacccctat ttgtttattt
     4201 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4261 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4321 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4381 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4441 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4501 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4561 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4621 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4681 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4741 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4801 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4861 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4921 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4981 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5041 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5101 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5161 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5221 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5281 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5341 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5401 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     5461 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5521 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5581 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5641 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5701 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5761 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5821 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5881 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5941 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6001 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6061 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6121 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6181 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6241 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6301 gaccatgagg cgcgccg
//