LOCUS       pDY0312_pCAG-PhiBT1_Int 6638 bp ds-DNA     circular     02-SEP-2021
DEFINITION  .
KEYWORDS    "accession:addgene_51271_256264"                                   
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct                                            
FEATURES             Location/Qualifiers
     source          1..6638
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     enhancer        6..385
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        387..664
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     intron          665..1681
                     /label="chimeric intron"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     1605..1627
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1684..1696
                     /label="F common for Bxb1 mutants"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1694..1696
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1694..1696
                     /label="T7"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1697..3573
                     /label="PhiBT1 Integrase"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             1729..3537
                     /label="Translation 1729-3537"
     primer_bind     complement(3574..3574)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     polyA_signal    3574..3787
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3859..3875)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(3859..3876)
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(3868..3890)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      4017..4445
                     /label="f1 ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(4104..4123)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     4314..4335
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        4498..4602
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4603..5463
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(4821..4840)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      5634..6222
                     /label="ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     6123..6142
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6376..6393
                     /label="L4440"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     protein_bind    6510..6531
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        6546..6576
                     /label="lac promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    6584..6600
                     /label="lac operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     6589..6611
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6608..6624
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     6608..6624
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 gattcgacat tgattattga ctagttatta atagtaatca attacggggt cattagttca
       61 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      121 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      181 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      241 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
      301 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
      361 cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc
      421 catctccccc ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc
      481 agcgatgggg gcgggggggg ggggggggcg cgcgccrggs ggggsggggs ggggsgrggg
      541 gsggggsggg gsgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa
      601 gtttcctttt atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg
      661 ggcgggagtc gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg
      721 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc
      781 tcctccgggc tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg
      841 tgaaagcctt gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc
      901 gtgcgtgtgt gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga
      961 gcgctgcggg cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc
     1021 cgggggcggt gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg
     1081 tgtgtgcgtg ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct
     1141 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg
     1201 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg
     1261 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc
     1321 cggcggctgt cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag
     1381 ggcgcaggga cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg
     1441 caccccctct agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg
     1501 ggagggcctt cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg
     1561 tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gatccttaat taataatacg actcactata gggggtcgac ccgccaccAT GCCCAAGAAG
     1741 AAACGGAAAG TGATGAGCCC CTTTATCGCC CCGGACGTGC CCGAGCACCT CCTGGACACT
     1801 GTGCGCGTCT TTCTGTACGC CCGTCAGAGT AAAGGACGGT CAGATGGATC TGACGTGTCC
     1861 ACCGAAGCAC AGCTCGCTGC CGGACGGGCC CTTGTTGCCT CAAGAAACGC ACAAGGGGGA
     1921 GCTAGATGGG TGGTGGCGGG CGAATTCGTG GATGTGGGCA GATCAGGGTG GGACCCGAAT
     1981 GTGACACGCG CCGACTTCGA AAGAATGATG GGCGAGGTGC GCGCCGGTGA GGGAGACGTA
     2041 GTGGTGGTTA ATGAACTGAG TCGCCTTACG AGGAAGGGCG CCCACGACGC TCTGGAGATC
     2101 GATAACGAAC TCAAAAAACA CGGTGTGCGG TTCATGAGCG TGCTGGAACC ATTCCTGGAT
     2161 ACCAGCACCC CAATCGGTGT CGCGATCTTT GCCCTGATTG CCGCGCTCGC TAAACAGGAT
     2221 TCAGACCTTA AAGCTGAGCG GCTGAAGGGG GCTAAAGATG AGATCGCTGC CTTGGGGGGT
     2281 GTGCACAGCT CATCTGCGCC ATTCGGCATG AGGGCGGTCA GAAAGAAAGT GGATAACCTG
     2341 GTCATATCTG TTCTGGAGCC TGATGAGGAC AACCCGGACC ACGTTGAGCT TGTGGAACGG
     2401 ATGGCTAAGA TGTCTTTCGA AGGCGTCAGC GATAACGCAA TTGCCACAAC ATTTGAGAAG
     2461 GAGAAAATCC CCTCTCCGGG GATGGCTGAG AGACGAGCCA CGGAGAAGAG GCTTGCTTCT
     2521 ATTAAGGCAC GGAGGCTCAA TGGCGCCGAA AAGCCGATCA TGTGGCGGGC GCAGACAGTT
     2581 AGATGGATTC TTAACCATCC CGCGATTGGT GGATTCGCAT TCGAGCGGGT GAAACACGGA
     2641 AAAGCCCACA TCAACGTGAT ACGAAGAGAT CCCGGCGGCA AACCCCTTAC CCCTCACACT
     2701 GGTATCCTGT CTGGATCCAA GTGGTTGGAA CTCCAGGAGA AGAGAAGCGG GAAAAATCTC
     2761 TCCGACCGCA AACCAGGTGC CGAAGTGGAA CCTACGCTGC TTTCCGGGTG GAGATTTCTG
     2821 GGATGTCGGA TATGCGGTGG GTCAATGGGC CAGTCCCAAG GGGGCCGTAA GAGGAATGGG
     2881 GACTTGGCTG AGGGCAATTA CATGTGTGCA AACCCAAAGG GGCACGGCGG TCTGAGCGTC
     2941 AAGAGGTCTG AGCTTGATGA ATTCGTGGCA TCAAAAGTCT GGGCCAGGTT GCGCACGGCT
     3001 GACATGGAGG ATGAACATGA CCAAGCATGG ATTGCAGCTG CAGCTGAACG GTTTGCTTTG
     3061 CAGCACGACC TGGCGGGGGT AGCTGACGAG CGACGGGAGC AACAAGCTCA CCTGGATAAC
     3121 GTTCGGAGAT CAATAAAAGA TCTCCAGGCG GATAGGAAGG CAGGTCTCTA CGTGGGACGC
     3181 GAAGAACTGG AGACCTGGCG CAGTACCGTC CTGCAATATA GGAGCTACGA GGCTGAGTGT
     3241 ACTACTAGGT TGGCTGAGCT GGATGAAAAA ATGAATGGAT CCACCCGGGT GCCTTCAGAA
     3301 TGGTTTAGCG GCGAGGACCC AACCGCGGAA GGAGGCATAT GGGCGAGCTG GGATGTCTAT
     3361 GAGCGCCGGG AGTTTCTCAG CTTTTTTTTG GACTCCGTAA TGGTTGACAG GGGCAGACAT
     3421 CCTGAAACCA AGAAATATAT ACCATTGAAA GACCGGGTGA CCTTAAAGTG GGCGGAGCTG
     3481 TTAAAGGAAG AGGATGAAGC AAGCGAGGCC ACAGAACGGG AGCTGGCAGC TCTTTAGgtc
     3541 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     3601 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     3661 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3721 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3781 tctatggctt ctgaggcgga aagaaccagc tggggctcga gatccactag ttctagcctc
     3841 gaggctagag cggccgccac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     3901 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     3961 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     4021 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     4081 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     4141 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     4201 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     4261 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     4321 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     4381 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     4441 gaattttaac aaaatattaa cgcttacrmk tymsrtkssm cwttymggsg aaatgtgcgc
     4501 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4561 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4621 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4681 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4741 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4801 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4861 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4921 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4981 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5041 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5101 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     5161 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5221 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5281 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5341 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5401 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5461 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5521 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5581 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5641 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5701 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5761 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     5821 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5881 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5941 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6001 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6061 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6121 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6181 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6241 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6301 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6361 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6421 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6481 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6541 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6601 tttcacacag gaaacagcta tgaccatgag gcgcgccg
//