LOCUS       pDY0739_aRY584_G2_integ 6311 bp ds-DNA     circular     08-SEP-2021
DEFINITION  .
ACCESSION   urn.local...1m-dg6hxcq                                             
KEYWORDS    "accession:addgene_51271_256264"                                   
SOURCE      synthetic DNA construct                                            
  ORGANISM  synthetic DNA construct.                                           
FEATURES             Location/Qualifiers
     protein_bind    16..37
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        52..82
                     /label="lac promoter"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    90..106
                     /label="lac operator"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     95..117
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     114..130
                     /label="M13 rev"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     114..130
                     /label="M13 Reverse"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     source          join(145..6311,1..144)
                     /label="source"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     enhancer        150..529
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     promoter        531..808
                     /label="chicken beta-actin promoter"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     intron          809..1825
                     /label="chimeric intron"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer_bind     1749..1771
                     /label="pCAGGS-5"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1828..1863
                     /label="F common for Bxb1 mutants"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     promoter        1838..1856
                     /label="T7 promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer_bind     1838..1857
                     /label="T7"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1841..1872
                     /label="5' Gibson"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     CDS             1873..3354
                     /label="Translation 1873-3354"
     misc_feature    1874..1903
                     /label="PD5642"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    1876..3351
                     /label="CCAAAAAAGAAAAGAAAAGTGTATCCCTATGATGTCCCCGATTATGCCGGTTCAAGAGCCCTGGTCGTGATTAGACTGAGCCGAGTGACAGACGCCACCACAAGTCCCGAGAGACAGCTGGAATCATGCCAGCAGCTCTGTGCTCAGCGGGGTTGGGATGTGGTCGGCGTGGCAGAGGATCTGGACGTGAGCGGGGCCGTCGATCCATTCGACAGAAAGAGGAGGCCCAACCTGGCAAGATGGCTCGCTTTCGAGGAACAGCCCTTTGATGTGATCGTCGCCTACAGAGTGGACCGGCTGACCCGCTCAATTCGACATCTCCAGCAGCTGGTGCATTGGGCTGAGGACCACAAGAAACTGGTGGTCAGCGCAACAGAAGCCCACTTCGATACTACCACACCTTTTGCCGCTGTGGTCATCGCACTGATGGGCACTGTGGCCCAGATGGAGCTCGAAGCTATCAAGGAGCGAAACAGGAGCGCAGCCCATTTCAATATTAGGGCCGGTAAATACAGAGGCTCCCTGCCCCCTTGGGGATATCTCCCTACCAGGGTGGATGGGGAGTGGAGACTGGTGCCAGACCCCGTCCAGAGAGAGCGGATTCTGGAAGTGTACCACAGAGTGGTCGATAACCACGAACCACTCCATCTGGTGGCACACGACCTGAATAGACGCGGCGTGCTCTCTCCAAAGGATTATTTTGCTCAGCTGCAGGGAAGAGAGCCACAGGGAAGAGAATGGAGTGCTACTGCACTGAAGAGATCTATGATCAGTGAGGCTATGCTGGGTTACGCAACACTCAATGGCAAAACTGTCCGGGACGATGACGGAGCCCCTCTGGTGAGGGCTGAGCCTATTCTCACCAGAGAGCAGCTCGAAGCTCTGCGGGCAGAACTGGTCAAGACTAGTCGCGCCAAACCTGCCGTGAGCACCCCAAGCCTGCTCCTGAGGGTGCTGTTCTGCGCCGTCTGTGGAGAGCCAGCATACAAGTTTGCCGGCGGAGGGCGCAAACATCCCCGCTATCGATGCAGGAGCATGGGGTTCCCTAAGCACTGTGGAAACGGGACAGTGGCCATGGCTGAGTGGGACGCCTTTTGCGAGGAACAGGTGCTGGATCTCCTGGGTGACGCTGAGCGGCTGGAAAAAGTGTGGGTGGCAGGATCTGACTCCGCTGTGGAGCTGGCAGAAGTCAATGCCGAGCTCGTGGATCTGACTTCCCTCATCGGATCTCCTGCATATAGAGCTGGGTCCCCACAGAGAGAAGCTCTGGACGCACGAATTGCTGCACTCGCTGCTAGACAGGAGGAACTGGAGGGCCTGGAGGCCAGGCCCTCTGGATGGGAGTGGCGAGAAACCGGACAGAGGTTTGGGGATTGGTGGAGGGAGCAGGACACCGCAGCCAAGAACACATGGCTGAGATCCATGAATGTCCGGCTCACATTCGACGTGCGCGGTGGCCTGACTCGAACCATCGATTTTGGCGACCTGCAGGAGTATGAACAGCACCTGAGACTGGGGTCCGTGGTCGAAAGACTGCACACTGGGATGTCC"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(3321..3352)
                     /label="PD5168"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3355..3390
                     /label="3' Gibson"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     complement(3374..3391)
                     /label="BGH-rev"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    complement(3376..3398)
                     /label="PD4560"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    3380..3604
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer_bind     complement(3676..3692)
                     /label="M13 fwd"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer_bind     complement(3676..3693)
                     /label="M13 Forward"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(3685..3707)
                     /label="M13/pUC Forward"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     rep_origin      3834..4262
                     /label="f1 ori"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer_bind     complement(3921..3940)
                     /label="F1ori-R"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer_bind     4131..4152
                     /label="F1ori-F"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     promoter        4315..4419
                     /label="AmpR promoter"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4420..5280
                     /label="AmpR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     complement(4638..4657)
                     /label="Amp-R"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     rep_origin      5451..6039
                     /label="ori"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer_bind     5940..5959
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer_bind     6193..6210
                     /label="L4440"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
       61 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
      121 cagctatgac catgaggcgc gccggattcg acattgatta ttgactagtt attaatagta
      181 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      241 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      301 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      361 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      421 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      481 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag
      541 ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt
      601 atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc
      661 rggsggggsg gggsggggsg rggggsgggg sggggsgagg cggagaggtg cggcggcagc
      721 caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc
      781 ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc
      841 ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca
      901 ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg tttaatgacg
      961 gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg gccctttgtg
     1021 cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc gtggggagcg ccgcgtgcgg
     1081 ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc gcggggcttt gtgcgctccg
     1141 cagtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg ggggctgcga
     1201 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1261 gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga gttgctgagc acggcccggc
     1321 ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg
     1381 gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg gctcggggga
     1441 ggggcgcggc ggcccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1501 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctgtgcgga
     1561 gccgaaatct gggaggcgcc gccgcacccc ctctagcggg cgcggggcga agcggtgcgg
     1621 cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc gccgtcccct
     1681 tctccctctc cagcctcggg gctgtccgcg gggggacggc tgccttcggg ggggacgggg
     1741 cagggcgggg ttcggcttct ggcgtgtgac cggcggctct agagcctctg ctaaccatgt
     1801 tcatgccttc ttctttttcc tacagatcct taattaataa tacgactcac tatagggggt
     1861 cgacccgcca ccatgaagga gaaggtgagt gagagaaaaa caggcgccat ttacataaga
     1921 gtttctacgg acaaacagga agagctttca ccagacgcac agctgaggct cctcctggac
     1981 tacgctaaga aagattctat cgatgttcct aaggagtaca tcttccaaga taacggcatt
     2041 agtgggcgaa aagcgaacaa gcgccccgcg ttccagaata tgatcgcact cgcgaagtcc
     2101 aaagagcacc caatcgacac aatcattgtg tggaagttct ctcgctttgc ccggaatcag
     2161 gaggaatcaa ttgtgtacaa gagtttactc aaaaaaaaca acgtcgatgt ggtgagtgtg
     2221 tccgagcctc tgattgatgg gccatttgga agcctgattg agagaattat tgagtggatg
     2281 gacgagtatt attccattcg attgtctggc gaggtgatgc gtggtatgac tcaaaatgcc
     2341 atgcgggggc attaccagag cgatgcaccg attgggtaca catccccagg ggacaaaaag
     2401 cccccggtta taaacccgga taccgttcag attcctctga tgatcaaaga tatgttctta
     2461 agcggctcaa cccagctgca aattgccaga aagctcaacg acagtggcta taggacaaag
     2521 cgcggtaacc tgtgggacgc gagaggcgtc cggtacgtcc tggagaaccc gttttacatc
     2581 gggaaaagcc gctggaatta cacggagaga gggcgacggc tgaagccggc agatgaggtg
     2641 atatacgctg acgggaactg ggaggcactg tgggatgagg acaccttcaa ggagatccaa
     2701 aaaagattgg cactgaatat gcgcaagtcc aagtctaggg acatctcagc tgcaaaacac
     2761 tggctgagcg gtctcttaat ctgttcttcc tgcggcggaa ccctggcctt cgggggagca
     2821 cacaatatga gggggtttca atgctggaaa tactcaaagg ggttctgcag cgaatcccat
     2881 tatatcagca ccggtccaat tgagaaaatg gttctggagt acttagaggc cgtcatgcac
     2941 tcccctgcgc tgagttacac ggttatcagt agttcatccg tcgatgccag ctccaaactg
     3001 tcagacctgg agcgccaatt gcagaaaata gacgccaagg agaaacgcat caaggcagca
     3061 tacctcaacg aaatagatac actggaggag tacaaagcta ataaaacagc cttggaggaa
     3121 gaacgccgta ccgtcgagaa ggaaatcgag gagctcaccc tcagcgacgt gaaatattct
     3181 aaggaggacc ttgacaagaa aatgaagcag aatatatcag acctgctgcg ggtgctgaga
     3241 gacgaatctg ccgattacat ccagaaaggt aacatgatga gaaacgtggt cgatcatatc
     3301 gtctttaaca ggaagaatac tagcctggac gtttttctga aattagtagt gtaggtcaga
     3361 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     3421 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     3481 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3541 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3601 atggcttctg aggcggaaag aaccagctgg ggctcgagat ccactagttc tagcctcgag
     3661 gctagagcgg ccgccactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     3721 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     3781 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     3841 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3901 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3961 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4021 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4081 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4141 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4201 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4261 ttttaacaaa atattaacgc ttacrmktym srtkssmcwt tymggsgaaa tgtgcgcgga
     4321 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4381 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4441 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4501 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4561 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4621 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     4681 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     4741 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     4801 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     4861 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     4921 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     4981 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5041 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5101 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5161 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5221 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5281 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5341 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5401 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5461 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5521 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5581 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     5641 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     5701 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     5761 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     5821 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     5881 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     5941 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6001 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6061 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6121 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6181 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6241 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6301 aaagcgggca g
//