LOCUS       pTriEx-FLAG-mCherry-Sspb        6105 bp    DNA     circular
            
ACCESSION   DNA_pTriEx-4
VERSION     DNA_pTriEx-4.0
KEYWORDS    .

COMMENT
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DISPLAY_SETUP_END|
COMMENT     
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FEATURES             Location/Qualifiers
     primer          1534..1554
                     /label=CMV F
                     /vntifkey="27"
     misc_feature    3379..3399
                     /label=DOWN
                     /type="misc_feature"
     misc_feature    2117..2140
                     /label=FLAG
                     /type="misc_feature"
     misc_feature    2153..2857
                     /label=mCherry
                     /type="misc_feature"
     misc_feature    2870..3208
                     /label=SspB
                     /type="misc_feature"
ORIGIN
        1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
       61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
      121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
      181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
      241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
      301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
      361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
      421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
      481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
      541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
      601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
      661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
      721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
      781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
      841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
      901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
      961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggcct
     1021 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     1081 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     1141 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     1201 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagtc
     1261 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     1321 ccttacggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgc
     1381 tgatgcggtt ttggcagtac accaatgggc gtggatagcg gtttgactca cggggatttc
     1441 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     1501 ttccaaaatg tcgtaataac cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt
     1561 gggaggtcta tataagcaga cgtcgtttag tgaaccgtca gatcactaga tgctttattg
     1621 cggtagttta tcacagttaa attgctaacg ccagtctcga acttaacgtg cagaagttgg
     1681 tcgtgaggca ctgggcaggt aagtatcggg ccctttgtgc ggggggagcg gctcggggct
     1741 gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1801 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1861 agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc
     1921 ggaccgaaat taatacgact cactataggg gaattgtgag cggataacaa ttccccggag
     1981 ttaatccggg acctttaatt caacccaaca caatatatta tagttaaata agaattatta
     2041 tcaaatcatt tgtatattaa ttaaaatact atactgtaaa ttacatttta tttacaatca
     2101 aaggagatat accatggact ataaggacga tgatgacaaa ggatctggat ctgtgagcaa
     2161 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     2221 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     2281 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     2341 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     2401 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     2461 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     2521 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     2581 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     2641 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     2701 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     2761 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     2821 ccgccactcc accggcggca tggacgagct gtacaagggt tctggatcca tgagctcccc
     2881 gaaacgccct aagctgctgc gtgaatatta cgattggctg gttgataaca gctttacccc
     2941 atatctggtg gtggatgcca catacctggg cgtgaacgtg cccgtggagt atgtgaaaga
     3001 cggtcagatc gtgctgaatc tgtctgcaag tgcgaccggc aacctgcaac tgacaaatga
     3061 ttttatccag ttcaacgccc gctttaaggg cgtgtctcgt gaactgtata tcccgatggg
     3121 tgccgctctg gccatttacg ctcgcgagaa cggcgatggt gtgatgttcg aaccagaaga
     3181 aatctatgac gagctgaata ttggtagatg aaagcttgcg gccgcacagc tgtatacacg
     3241 tgcaagccag ccagaactcg ccccggaaga ccccgaggat ctcgagcacc accatcacca
     3301 tcaccatcac taagtgatta acctcaggtg caggctgcct atcagaaggt ggtggctggt
     3361 gtggccaatg ccctggctca caaataccac tgagatcgat ctttttccct ctgccaaaaa
     3421 ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat
     3481 tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag
     3541 ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca
     3601 tatgtaacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
     3661 agcatgcgga ggaaattctc cttgaagttt ccctggtgtt caaagtaaag gagtttgcac
     3721 cagacgcacc tctgttcact ggtccggcgt attaaaacac gatacattgt tattagtaca
     3781 tttattaagc gctagattct gtgcgttgtt gatttacaga caattgttgt acgtatttta
     3841 ataattcatt aaatttataa tctttagggt ggtatgttag agcgaaaatc aaatgatttt
     3901 cagcgtcttt atatctgaat ttaaatatta aatcctcaat agatttgtaa aataggtttc
     3961 gattagtttc aaacaagggt tgtttttccg aaccgatggc tggactatct aatggatttt
     4021 cgctcaacgc cacaaaactt gccaaatctt gtagcagcaa tctagctttg tcgatattcg
     4081 tttgtgtttt gttttgtaat aaaggttcga cgtcgttcaa aatattatgc gcttttgtat
     4141 ttctttcatc actgtcgtta gtgtacaatt gactcgacgt aaacacgtta aatagagctt
     4201 ggacatattt aacatcgggc gtgttagctt tattaggccg attatcgtcg tcgtcccaac
     4261 cctcgtcgtt agaagttgct tccgaagacg attttgccat agccacacga cgcctattaa
     4321 ttgtgtcggc taacacgtcc gcgatcaaat ttgtagttga gctttttgga attgcgatcg
     4381 cataacttcg tatagcatac attatacgaa gttataagct cggaacgctg cgctcggtcg
     4441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4561 aaaaggccgc gttgctggcg tttttccata ggctccgccc cctgacgagc atcacaaaaa
     4621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     4681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     4741 cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag
     4801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     4861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     4921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     4981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     5041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5161 aggatctcaa gaagatcctt tgttaccaat gcttaatcag tgaggcacct atctcagcga
     5221 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     5281 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     5341 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     5401 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     5461 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     5521 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     5581 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     5641 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     5701 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     5761 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     5821 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     5881 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     5941 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     6001 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     6061 attattgaag catttatcag ggttattgtc tcatgtccgc gcgtt
//
