LOCUS       pBTK800                 8414 bp ds-DNA     circular     22-DEC-2021
DEFINITION  .
ACCESSION   urn.local...z-caqd7rd                                              
FEATURES             Location/Qualifiers
     misc_feature    complement(1112..1126)
                     /label="polyArg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     rep_origin      complement(2091..2178)
                     /label="RSF1010 oriT"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     rep_origin      complement(2520..2914)
                     /label="RSF1010 oriV"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          3293..3310
                     /label="pMMB67EH F"
                     /note="sequence: CACCTGGTCGCTTTCAGA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    3383..3525
                     /label="ConLS'"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(3530..3611)
                     /label="rnpB_T1"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     promoter        3612..3670
                     /label="CP25 promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    3672..3682
                     /label="Type 3 dropout"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        3683..3812
                     /label="BBa_J72163 GlpT Promoter"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3683..4694
                     /label="dropout region"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    3813..3848
                     /label="sfGFP Ribosome Binding Site"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3849..4565
                     /label="sfGFP"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     terminator      4566..4694
                     /label="BBa_B0015 Terminator"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4695..4705
                     /label="Type 3 dropout DS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        complement(4706..4764)
                     /label="CP25 promoter"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     terminator      4765..4847
                     /label="rpoC"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    4859..4923
                     /label="5-ConRE'-7"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    complement(4936..5946)
                     /label="SpecR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5832..5851
                     /label="mb_SpecR_Ori_Seam"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        complement(5947..6080)
                     /label="SpecR Promoter"
                     /ApEinfo_revcolor=#cbb300
                     /ApEinfo_fwdcolor=#cbb300
     misc_feature    complement(6107..7057)
                     /label="RSF1010 RepC"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    complement(6945..7784)
                     /label="RSF1010 RepA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(join(8295..8414,1..852))
                     /label="RSF1010 RepB"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
ORIGIN
        1 ctcgatgtaa tccgcttcgt ggccgggctt gcgctctgcc agcgctgggc tggcctcggc
       61 catggccttg ccgatttcct cggcactgcg gccccggctg gccagcttct gcgcggcgat
      121 aaagtcgcac ttgctgaggt catgaccgaa gcgcttgacc agcccggcca tctcgctgcg
      181 gtactcgtcc agcgccgtgc gccggtggcg gctaagctgc cgctcgggca gttcgaggct
      241 ggccagcctg cgggccttct cctgctgccg ctgggcctgc tcgatctgct ggccagcctg
      301 ctgcaccagc gccgggccag cggtggcggt cttgcccttg gattcacgca gcagcaccca
      361 cggctgataa ccggcgcggg tggtgtgctt gtccttgcgg ttggtgaagc ccgccaagcg
      421 gccatagtgg cggctgtcgg cgctggccgg gtcggcgtcg tactcgctgg ccagcgtccg
      481 ggcaatctgc ccccgaagtt caccgcctgc ggcgtcggcc accttgaccc atgcctgata
      541 gttcttcggg ctggtttcca ctaccagggc aggctcccgg ccctcggctt tcatgtcatc
      601 caggtcaaac tcgctgaggt cgtccaccag caccagacca tgccgctcct gctcggcggg
      661 cctgatatac acgtcattgc cctgggcatt catccgcttg agccatggcg tgttctggag
      721 cacttcggcg gctgaccatt cccggttcat catctggccg gtgggtgcgt ccctgacgcc
      781 gatatcgaag cgctcacagc ccatggcctt gagctgtcgg cctatggcct gcaaagtcct
      841 gtcgttcttc atcgggccac caagcgcagc cagatcgagc cgtcctcggt tgtcagtggc
      901 gtcaggtcga gcaagagcaa cgatgcgatc agcagcacca ccgtaggcat catggaagcc
      961 agcatcacgg ttagccatag cttccagtgc cacccccgcg acgcgctccg ggcgctctgc
     1021 gcggcgctgc tcacctcggc ggctacctcc cgcaactctt tggccagctc cacccatgcc
     1081 gcccctgtct ggcgctgggc tttcagccac tccgccgcct gcgcctcgct ggcctgcttg
     1141 gtctggctca tgacctgccg ggcttcgtcg gccagtgtcg ccatgctctg ggccagcggt
     1201 tcgatctgct ccgctaactc gttgatgcct ctggatttct tcactctgtc gattgcgttc
     1261 atggtctatt gcctcccggt attcctgtaa gtcgatgatc tgggcgttgg cggtgtcgat
     1321 gttcagggcc acgtctgccc ggtcggtgcg gatgccccgg ccttccatct ccaccacgtt
     1381 cggccccagg tgaacaccgg gcaggcgctc gatgccctgc gcctcaagtg ttctgtggtc
     1441 aatgcgggcg tcgtggccag cccgctctaa tgcccggttg gcatggtcgg cccatgcctc
     1501 gcgggtctgc tcaagccatg ccttgggctt gagcgcttcg gtcttctgtg ccccgccctt
     1561 ctccggggtc ttgccgttgt accgcttgaa ccactgagcg gcgggccgct cgatgccgtc
     1621 attgatccgc tcggagatca tcaggtggca gtgcgggttc tcgccgccac cggcatggat
     1681 ggccagcgta tacggcaggc gctcggcacc ggtcaggtgc tgggcgaact cggacgccag
     1741 cgccttctgc tggtcgaggg tcagctcgac cggcagggca aattcgacct ccttgaacag
     1801 ccgcccattg gcgcgttcat acaggtcggc agcatcccag tagtcggcgg gccgctcgac
     1861 gaactccggc atgtgcccgg attcggcgtg caagacttca tccatgtcgc gggcatactt
     1921 gccttcgcgc tggatgtagt cggccttggc cctggccgat tggccgcccg acctgctgcc
     1981 ggttttcgcc gtaaggtgat aaatcgccat gctgcctcgc tgttgctttt gcttttcggc
     2041 tccatgcaat ggccctcgga gagcgcaccg cccgaagggt ggccgttagg ccagtttctc
     2101 gaagagaaac cggtaagtgc gccctcccct acaaagtagg gtcgggattg ccgccgctgt
     2161 gcctccatga tagcctacga gacagcacat taacaatggg gtgtcaagat ggttaagggg
     2221 agcaacaagg cggcggatcg gctggccaag ctcgaagaac aacgagcgcg aatcaatgcc
     2281 gaaattcagc gggagcgggc aagggaacag cagcaagagc gcaagaacga aacaaggcgc
     2341 aaggtgctgg tgggggccat gattttggcc aaggtgaaca gcagcgagtg gccggaggat
     2401 cggctcatgg cggcaatgga tgcgtacctt gaacgcgacc acgaccgcgc cttgttcggt
     2461 ctgccgccac gccagaagga tgagccgggc tgaatgatcg accgagacag gccctgcggg
     2521 gctgcacacg cgcccccacc cttcgggtag ggggaaaggc cgctaaagcg gctaaaagcg
     2581 ctccagcgta tttctgcggg gtttggtgtg gggtttagcg ggctttgccc gcctttcccc
     2641 ctgccgcgca gcggtggggc ggtgtgtagc ctagcgcagc gaatagacca gctatccggc
     2701 ctctggccgg gcatattggg caagggcagc agcgccccac aagggcgctg ataaccgcgc
     2761 ctagtggatt attcttagat aatcatggat ggatttttcc aacaccccgc cagcccccgc
     2821 ccctgctggg tttgcaggtt tgggggcgtg acagttattg caggggttcg tgacagttat
     2881 tgcagggggg cgtgacagtt attgcagggg ttcgtgacag ttagtacggg agtgacgggc
     2941 actggctggc aatgtctagc aacggcaggc atttcggctg agggtaaaag aactttccgc
     3001 taagcgatag actgtatgta aacacagtat tgcaaggacg cggaacatgc ctcatgtggc
     3061 ggccaggacg gccagccggg atcgggatac tggtcgttac cagagccacc gacccgagca
     3121 aacccttctc tatcagatcg ttgacgagta ttacccggca ttcgctgcgc ttatggcaga
     3181 gcagggaaag gaattgccgg gctatgtgca acgggaattt gaagaatttc tccaatgcgg
     3241 gcggctggag catggctttc tacgggttcg ctgcgagtct tgccacgccg agcacctggt
     3301 cgctttcaga aatcaatcta aagtatatat gagtaaactt ggtctgacag gcccctgaat
     3361 tcgcatctag actgatgaga cgtggtagag ccacaaacag ccggtacaag caacgatctc
     3421 caggaccatc tgaatcatgc gcggatgaca cgaactcacg acggcgatca cagacattaa
     3481 cccacagtac agacactgcg acaacgtggc aattcgtcgc aatacaacgg acagtcattc
     3541 atctttctgc ccctccaaaa gcaaaaaccc gccgaagcgg gtttttacgt aaatcaggtg
     3601 aaactgaccg actttggcag tttattcttg acatgtagtg agggggctgg tataatcaca
     3661 tagtactgtt ttatgtgaga ccgaaagtga aacgtgattt catgcgtcat tttgaacatt
     3721 ttgtaaatct tatttaataa tgtgtgcggc aattcacatt taatttatga atgttttctt
     3781 aacatcgcgg caactcaaga aacggcaggt tcggatctta gctactagag aaagaggaga
     3841 aatactagat gcgtaaaggc gaagagctgt tcactggtgt cgtccctatt ctggtggaac
     3901 tggatggtga tgtcaacggt cataagtttt ccgtgcgtgg cgagggtgaa ggtgacgcaa
     3961 ctaatggtaa actgacgctg aagttcatct gtactactgg taaactgccg gttccttggc
     4021 cgactctggt aacgacgctg acttatggtg ttcagtgctt tgctcgttat ccggaccata
     4081 tgaagcagca tgacttcttc aagtccgcca tgccggaagg ctatgtgcag gaacgcacga
     4141 tttcctttaa ggatgacggc acgtacaaaa cgcgtgcgga agtgaaattt gaaggcgata
     4201 ccctggtaaa ccgcattgag ctgaaaggca ttgactttaa agaggacggc aatatcctgg
     4261 gccataagct ggaatacaat tttaacagcc acaatgttta catcaccgcc gataaacaaa
     4321 aaaatggcat taaagcgaat tttaaaattc gccacaacgt ggaggatggc agcgtgcagc
     4381 tggctgatca ctaccagcaa aacactccaa tcggtgatgg tcctgttctg ctgccagaca
     4441 atcactatct gagcacgcaa agcgttctgt ctaaagatcc gaacgagaaa cgcgatcata
     4501 tggttctgct ggagttcgta accgcagcgg gcatcacgca tggtatggat gaactgtaca
     4561 aatgaccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     4621 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     4681 tttctgcgtt tataggtctc aatccaacag tactatgtga ttataccagc cccctcacta
     4741 catgtcaaga ataaactgcc aaaggtaatc gttaatccgc aaataacgta aaaacccgct
     4801 tcggcgggtt tttttatggg gggagtttag ggaaagagca tttgtcagct gcgtctcaag
     4861 cagttacaga gatgttacga acccccagga catccgagaa tgcgaggcga tggagggtac
     4921 aacccgagcg gccgcttatt tgccgactac cttggtgatc tcgcctttca cgtagtggac
     4981 aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc caagataagc
     5041 ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca ttgcccagtc
     5101 ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa tgcgggacaa
     5161 cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc atagcgttaa
     5221 ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga gttcctccgc
     5281 cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga tagccagatc
     5341 aatgtcgatc gtggctggct cgaagatacc cgcaagaatg tcattgcgct gccattctcc
     5401 aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt gcacaacaat
     5461 ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag tttccaaaag
     5521 gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg taaccagcaa
     5581 atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca aatgtacggc
     5641 cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata gttgagtcga
     5701 tacttcggcg atcaccgctt ccctcataat gtttaacttt gttttagggc gactgccctg
     5761 ctgcgtaaca tcgttgctgc tccataacat caaacatcga cccacggcgt aacgcgcttg
     5821 ctgcttggat gcccgaggca tagactgtac cccaaaaaaa cagtcataac aagccatgaa
     5881 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
     5941 gcgcatacgc tacttgcatt acagcttacg aaccgaacag gcttatgtcc actgggttcg
     6001 tgccttcatc cgtttccacg gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga
     6061 ggcatttctg tcctggctgg tcatgaccaa aatcccttaa cgtgagtcag cctgccgcct
     6121 tgggccgggt gatgtcgtac ttgcccgccg cgaactcggt taccgtccag cccagcgcga
     6181 ccagctccgg caacgcctcg cgcacccgct tgcggcgctt gcgcatggtc gaaccactgg
     6241 cctctgacgg ccagacatag ccgcacaagg tatctatgga agccttgccg gttttgccgg
     6301 ggtcgatcca gccacacagc cgctggtgca gcaggcgggc ggtttcgctg tccagcgccc
     6361 gcacctcgtc catgctgatg cgcacatgct ggccgccacc catgacggcc tgcgcgatca
     6421 aggggttcag ggccacgtac aggcgcccgt ccgcctcgtc gctggcgtac tccgacagca
     6481 gccgaaaccc ctgccgcttg cggccattct gggcgatgat ggataccttc caaaggcgct
     6541 cgatgcagtc ctgtatgtgc ttgagcgccc caccactatc gacctctgcc ccgatttcct
     6601 ttgccagcgc ccgatagcta cctttgacca catggcattc agcggtgacg gcctcccact
     6661 tgggttccag gaacagccgg agctgccgtc cgccttcggt cttgggttcc gggccaagca
     6721 ctaggccatt aggcccagcc atggccacca gcccttgcag gatgcgcaga tcatcagcgc
     6781 ccagcggctc cgggccgctg aactcgatcc gcttgccgtc gccgtagtca tacgtcacgt
     6841 ccagcttgct gcgcttgcgc tcgccccgct tgagggcacg gaacaggccg ggggccagac
     6901 agtgcgccgg gtcgtgccgg acgtggctga ggctgtgctt gttcttaggc ttcaccacgg
     6961 ggcaccccct tgctcttgcg ctgcctctcc agcacggcgg gcttgagcac cccgccgtca
     7021 tgccgcctga accaccgatc agcgaacggt gcgccatagt tggccttgct cacaccgaag
     7081 cggacgaaga accggcgctg gtcgtcgtcc acaccccatt cctcggcctc ggcgctggtc
     7141 atgctcgaca ggtaggactg ccagcggatg ttatcgacca gtaccgagct gccccggctg
     7201 gcctgctgct ggtcgcctgc gcccatcatg gccgcgccct tgctggcatg gtgcaggaac
     7261 acgatagagc acccggtatc ggcggcgatg gcctccatgc gaccgatgac ctgggccatg
     7321 gggccgctgg cgttttcttc ctcgatgtgg aaccggcgca gcgtgtccag caccatcagg
     7381 cggcggccct cggcggcgcg cttgaggccg tcgaaccact ccggggccat gatgttgggc
     7441 aggctgccga tcagcggctg gatcagcagg ccgtcagcca cggcttgccg ttcctcggcg
     7501 ctgaggtgcg ccccaagggc gtgcaggcgg tgatgaatgg cggtgggcgg gtcttcggcg
     7561 ggcaggtaga tcaccgggcc ggtgggcagt tcgcccacct ccagcagatc cggcccgcct
     7621 gcaatctgtg cggccagttg cagggccagc atggatttac cggcaccacc gggcgacacc
     7681 agcgccccga ccgtaccggc caccatgttg ggcaaaacgt agtccagcgg tggcggcgct
     7741 gctgcgaacg cctccagaat attgataggc ttatgggtag ccattgattg cctcctttgc
     7801 aggcagttgg tggttaggcg ctggcggggt cactaccccc gccctgcgcc gctctgagtt
     7861 cttccaggca ctcgcgcagc gcctcgtatt cgtcgtcggt cagccagaac ttgcgctgac
     7921 gcatcccttt ggccttcatg cgctcggcat atcgcgcttg gcgtacagcg tcagggctgg
     7981 ccagcaggtc gccggtctgc ttgtcctttt ggtctttcat atcagtcacc gagaaacttg
     8041 ccggggccga aaggcttgtc ttcgcggaac aaggacaagg tgcagccgtc aaggttaagg
     8101 ctggccatat cagcgactga aaagcggcca gcctcggcct tgtttgacgt ataaccaaag
     8161 ccaccgggca accaatagcc cttgtcactt ttgatcaggt agaccgaccc tgaagcgctt
     8221 ttttcgtatt ccataaaacc cccttctgtg cgtgagtact catagtataa caggcgtgag
     8281 taccaacgca agcactacat gctgaaatct ggcccgcccc tgtccatgcc tcgctggcgg
     8341 ggtgccggtg cccgtgccag ctcggcccgc gcaagctgga cgctgggcag acccatgacc
     8401 ttgctgacgg tgcg
//