LOCUS       Exported               10915 bp DNA     circular SYN 16-OCT-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10915)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10915
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     3385..3429
                     /label=fragment for
                     /note="color: black; sequence: 
                     cttccatttcaggtgtcgtgaGGTTTAGTGAACCGTCAGATCCGC"
     primer_bind     complement(3385..3429)
                     /label=vector rev
                     /note="color: black; sequence: 
                     GCGGATCTGACGGTTCACTAAACCtcacgacacctgaaatggaag"
     primer_bind     3407..3419
                     /label=pCEP-F
                     /note="color: black; sequence: AGAGCTCGTTTAGTGAACCG"
     primer_bind     3418..3469
                     /label=pmRubby3-F-donnor
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCatggtgtctaagggcgaaga"
     primer_bind     3418..3469
                     /label=pmNeonGreen-F-donnor
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCatggtgtccaagggcgaaga"
     primer_bind     3440..3471
                     /label=28a_green_ddx21_dat_ruby3_F_bamhi
                     /note="color: black; sequence: 
                     acagcaaatgggtcgcggatccatggtgtccaagggcgaagagg"
     primer_bind     3448..3471
                     /label=28a_green_ddx21_dat_ruby3_F_bamhi
                     /note="color: black; sequence: 
                     acagcaaatgggtcgcggatccatggtgtccaagggcgaagagg"
     CDS             3450..4157
                     /label=mNeongreen
                     /note="modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(4138..4195)
                     /label=pmRubby3-R-donnor
                     /note="color: black; sequence: 
                     GCAGAATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc"
     primer_bind     complement(4138..4195)
                     /label=pmNeonGreen-R-donnor
                     /note="color: black; sequence: 
                     GCAGAATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc"
     misc_feature    4172..4203
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     primer_bind     4187..4230
                     /label=DDX21-DAT-F-KPNI
                     /note="color: black; sequence: 
                     GAATTCTGCAGTCGACGGTACCCCGGGAAAACTCCGTAGTGACG"
     misc_feature    4189..4203
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     4191..4230
                     /label=p23-puro-DDX21-Ruby3-F_nhei
                     /note="color: black; sequence: 
                     gccgcaccggtctgcagctagcATGCCGGGAAAACTCCGTAGTGACG"
     misc_feature    4209..6554
                     /label=DDX21-DAT
                     /note="color: #a6acb3"
     CDS             4209..6554
                     /codon_start=1
                     /label=DDX21
                     /note="color: #993366"
                     /translation="PGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAE
                     EEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKK
                     VVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPS
                     EAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSG
                     KDLIAQARTGTDATFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSD
                     ITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEV
                     DQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLI
                     GKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSA
                     IKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDV
                     ESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSK
                     DAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGF
                     VTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQE
                     KWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGP
                     RGQRSGGGNKSNRSQNKGQKRSFSKAFGQ"
     misc_feature    4209..6554
                     /label=DDX21
                     /note="color: #a6acb3"
     primer_bind     4209..4230
                     /label=p23-puro-DDX21-Ruby3-F_nhei
                     /note="color: black; sequence: 
                     gccgcaccggtctgcagctagcATGCCGGGAAAACTCCGTAGTGACG"
     primer_bind     4814..4833
                     /label=Primer 1
                     /note="color: black; sequence: CCGAGGAGTGACCTTCCTAT"
     misc_feature    4854..5393
                     /label=DDX21-D1
                     /note="color: #a6acb3"
     primer_bind     4886..4929
                     /label=DDX21-GKT-DAT-F
                     /note="color: black; sequence: 
                     TGCACAGGCACGGACAGGAACTGACGCTACATTCTCCTTTGCCA"
     primer_bind     complement(4886..4929)
                     /label=DDX21-GKT-DAT-R
                     /note="color: black; sequence: 
                     TGGCAAAGGAGAATGTAGCGTCAGTTCCTGTCCGTGCCTGTGCA"
     misc_feature    4908..4913
                     /label=DAT-235-236
                     /note="color: #a6acb3"
     primer_bind     4989..5008
                     /label=DDX21-detect-F
                     /note="color: black; sequence: CAGGTACTGGTTCTTGCACC"
     primer_bind     5067..5086
                     /label=DDX21-seq-F
                     /note="color: black; sequence: TCAGTGGCTTGTTTTTATGG"
     primer_bind     complement(5089..5108)
                     /label=DDX21-detect-R1
                     /note="color: black; sequence: TTGACCTCCATAGGGAGTTC"
     primer_bind     complement(5202..5244)
                     /label=E340A-R
                     /note="color: black; sequence: 
                     TATCCAACATCTGGTCCACGGCATCCAGGACAACATGCTTAAG"
     primer_bind     5204..5248
                     /label=E340A-F
                     /note="color: black; sequence: 
                     TAAGCATGTTGTCCTGGATGCCGTGGACCAGATGTTGGATATGGG"
     misc_feature    5394..5924
                     /label=DDX21-D2
                     /note="color: #a6acb3"
     primer_bind     5813..5832
                     /label=DDX21-detect-F2
                     /note="color: black; sequence: GTGCATCTGCTTTTATCAGC"
     primer_bind     complement(5986..6005)
                     /label=DDX21-seq-R2
                     /note="color: black; sequence: CTTCTCAGCTGATTGTTTGA"
     primer_bind     complement(6532..6554)
                     /label=DDX21-DAT-XbaI-R
                     /note="color: black; sequence: 
                     cgttaggggggggggagggatcctctagattaTTATTGACCAAATGCTTTACTGAAAC"
     primer_bind     6533..6576
                     /label=DDX21-Ruby3-F2-overlap
                     /note="color: black; sequence: 
                     TTTCAGTAAAGCATTTGGTCAAatggtgtctaagggcgaagagc"
     primer_bind     complement(6533..6576)
                     /label=DDX21-Ruby3-R1-overlap
                     /note="color: black; sequence: 
                     gctcttcgcccttagacaccatTTGACCAAATGCTTTACTGAAA"
     primer_bind     complement(6533..6554)
                     /label=DDX21-DAT-R-BAMHI
                     /note="color: black; sequence: 
                     gTTATCTAGATCCGGTGGATCCTTATTGACCAAATGCTTTACTGAAA"
     misc_feature    6554
                     /label=????
                     /note="color: #a6acb3"
     CDS             6555..7265
                     /label=mRuby3
                     /note="created_by=User
                     modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     6555..6574
                     /label=pmRubby3-F-donnor
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCatggtgtctaagggcgaaga"
     primer_bind     6555..6574
                     /label=pmNeonGreen-F-donnor
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCatggtgtccaagggcgaaga"
     misc_feature    6678..6680
                     /label=T38V
                     /note="created_by=User"
                     /note="color: #a6acb3"
     primer_bind     complement(7244..7287)
                     /label=DDX21-Ruby3-R2-overlap_bamhi
                     /note="color: black; sequence: 
                     gTTATCTAGATCCGGTGGATCCcttgtacagctcgtccatgcca"
     primer_bind     complement(7244..7266)
                     /label=28a_green_ddx21_dat_ruby3_R_NOTI
                     /note="color: black; sequence: 
                     ggtggtgctcgagtgcggccgccttgtacagctcgtccatgcca"
     primer_bind     complement(7244..7265)
                     /label=p23-puro-DDX21-Ruby3-R_Xbai
                     /note="color: black; sequence: 
                     ggagaggggcagatccTCTAGacttgtacagctcgtccatgcca"
     primer_bind     complement(7246..7265)
                     /label=pmRubby3-R-donnor
                     /note="color: black; sequence: 
                     GCAGAATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc"
     primer_bind     complement(7246..7265)
                     /label=pmNeonGreen-R-donnor
                     /note="color: black; sequence: 
                     GCAGAATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc"
     primer_bind     complement(7266..7311)
                     /label=fragment rev
                     /note="color: black; sequence: 
                     gttgattaggatctatcgATgggcgTTATCTAGATCCGGTGGATCC"
     primer_bind     7266..7311
                     /label=vector for
                     /note="color: black; sequence: 
                     GGATCCACCGGATCTAGATAAcgcccATcgatagatcctaatcaac"
     primer_bind     complement(7266..7287)
                     /label=DDX21-DAT-R-BAMHI
                     /note="color: black; sequence: 
                     gTTATCTAGATCCGGTGGATCCTTATTGACCAAATGCTTTACTGAAA"
     misc_feature    7267..7285
                     /label=Feature 21
                     /note="color: #a6acb3"
     misc_feature    7267..7271
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     CDS             7286
                     /codon_start=1
                     /label=PuroR
                     /note="color: #ccffcc"
                     /translation=""
     misc_feature    7305..7893
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7776..7787)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7775"
                     /translation="IEGR"
     LTR             7968..8201
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     promoter        8406..8510
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             8511..9371
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8511 .. 8579 / #ccffcc / signal sequence
                        2: 8580 .. 9371 / #ccffcc
                     Cleavage site after base 8579"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9542..10130
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    10541..10562
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        10577..10607
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 10577 .. 10582 / #ffffff / -35
                        2: 10583 .. 10600 / #ffffff
                        3: 10601 .. 10607 / #ffffff / -10"
     protein_bind    10615..10631
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     10639..10655
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggttt agtgaaccgt
     3421 cagatccgct agcgctaccg gtcgccacca tggtgtccaa gggcgaagag gacaacatgg
     3481 ccagcctgcc tgccacccac gagctgcaca tcttcggcag catcaacggc gtggacttcg
     3541 acatggtggg acagggcacc ggcaacccca acgacggcta cgaggaactg aacctgaagt
     3601 ccacaaaggg cgacctgcag ttcagcccct ggattctggt gccccacatc ggctacggct
     3661 tccaccagta cctgccctac cccgacggca tgagcccttt tcaggccgcc atggtggatg
     3721 gcagcggcta ccaggtgcac cggaccatgc agtttgagga cggcgccagc ctgaccgtga
     3781 actaccggta cacatacgag ggcagccaca tcaagggcga ggcccaagtg aagggcacag
     3841 gctttccagc cgacggcccc gtgatgacca atagcctgac agccgccgac tggtgcagaa
     3901 gcaagaaaac ctaccccaat gacaagacca tcatcagcac cttcaagtgg tcctacacca
     3961 ccggcaatgg caagcggtac agaagcaccg cccggaccac ctacaccttc gccaaaccca
     4021 tggccgccaa ctacctgaag aaccagccta tgtacgtgtt ccgcaagacc gagctgaagc
     4081 actccaagac agaactgaac ttcaaagagt ggcagaaagc cttcaccgac gtgatgggca
     4141 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
     4201 acggtacccc gggaaaactc cgtagtgacg ctggtttgga atcagacacc gcaatgaaaa
     4261 aaggggagac actgcgaaag caaaccgagg agaaagagaa aaaagagaag ccaaaatctg
     4321 ataagactga agagatagca gaagaggaag aaactgtttt ccccaaagct aaacaagtta
     4381 aaaagaaagc agagccttct gaagttgaca tgaattctcc taaatccaaa aaggcaaaaa
     4441 agaaagagga gccatctcaa aatgacattt ctcctaaaac caaaagtttg agaaagaaaa
     4501 aggagcccat tgaaaagaaa gtggtttctt ctaaaaccaa aaaagtgaca aaaaatgagg
     4561 agccttctga ggaagaaata gatgctccta agcccaagaa gatgaagaaa gaaaaggaaa
     4621 tgaatggaga aactagagag aaaagcccca aactgaagaa tggatttcct catcctgaac
     4681 cggactgtaa ccccagtgaa gctgccagtg aagaaagtaa cagtgagata gagcaggaaa
     4741 tacctgtgga acaaaaagaa ggcgctttct ctaattttcc catatctgaa gaaactatta
     4801 aacttctcaa aggccgagga gtgaccttcc tatttcctat acaagcaaag acattccatc
     4861 atgtttacag cgggaaggac ttaattgcac aggcacggac aggaactgac gctacattct
     4921 cctttgccat ccctttgatt gagaaacttc atggggaact gcaagacagg aagagaggcc
     4981 gtgcccctca ggtactggtt cttgcaccta caagagagtt ggcaaatcaa gtaagcaaag
     5041 acttcagtga catcacaaaa aagctgtcag tggcttgttt ttatggtgga actccctatg
     5101 gaggtcaatt tgaacgcatg aggaatggga ttgatatcct ggttggaaca ccaggtcgta
     5161 tcaaagacca catacagaat ggcaaactag atctcaccaa acttaagcat gttgtcctgg
     5221 atgaagtgga ccagatgttg gatatgggat ttgctgatca agtggaagag attttaagtg
     5281 tggcatacaa gaaagattct gaagacaatc cccaaacatt gcttttttct gcaacttgcc
     5341 ctcattgggt atttaatgtt gccaagaaat acatgaaatc tacatatgaa caggtggacc
     5401 tgattggtaa aaagactcag aaaacggcaa taactgtgga gcatctggct attaagtgcc
     5461 actggactca gagggcagca gttattgggg atgtcatccg agtatatagt ggtcatcaag
     5521 gacgcactat catcttttgt gaaaccaaga aagaagccca ggagctgtcc cagaattcag
     5581 ctataaagca ggatgctcag tccttgcatg gagacattcc acagaagcaa agggaaatca
     5641 ccctgaaagg ttttagaaat ggtagttttg gagttttggt ggcaaccaat gttgctgcac
     5701 gtgggttaga catccctgag gttgatttgg ttatacaaag ctctccacca aaggatgtag
     5761 agtcctacat tcatcgatcc gggcggacag gcagagctgg aaggacgggg gtgtgcatct
     5821 gcttttatca gcacaaggaa gaatatcagt tagtacaagt ggagcaaaaa gcgggaatta
     5881 agttcaaacg aataggtgtt ccttctgcaa cagaaataat aaaagcttcc agcaaagatg
     5941 ccatcaggct tttggattcc gtgcctccca ctgccattag tcacttcaaa caatcagctg
     6001 agaagctgat agaggagaag ggagctgtgg aagctctggc agcagcactg gcccatattt
     6061 caggtgccac gtccgtagac cagcgctcct tgatcaactc aaatgtgggt tttgtgacca
     6121 tgatcttgca gtgctcaatt gaaatgccaa atattagtta tgcttggaaa gaacttaaag
     6181 agcagctggg cgaggagatt gattccaaag tgaagggaat ggtttttctc aaaggaaagc
     6241 tgggtgtttg ctttgatgta cctaccgcat cagtaacaga aatacaggag aaatggcatg
     6301 attcacgacg ctggcagctc tctgtggcca cagagcaacc agaactggaa ggaccacggg
     6361 aaggatatgg aggcttcagg ggacagcggg aaggcagtcg aggcttcagg ggacagcggg
     6421 acggaaacag aagattcaga ggacagcggg aaggcagtag aggcccgaga ggacagcgat
     6481 caggaggtgg caacaaaagt aacagatccc aaaacaaagg ccagaagcgg agtttcagta
     6541 aagcatttgg tcaaatggtg tctaagggcg aagagctgat caaggaaaat atgcgtatga
     6601 aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt gaaggagaag
     6661 gcagaccgta cgagggagtg caaaccatga ggatcaaagt catcgaggga ggacccctgc
     6721 catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtacc tttatcaagt
     6781 acccggccga catccctgat ttctttaaac agtcctttcc tgagggtttt acttgggaaa
     6841 gagttacgag atacgaagat ggtggagtcg tcaccgtcac gcaggacacc agccttgagg
     6901 atggcgagct cgtctacaac gtcaaggtca gaggggtaaa ctttccctcc aatggtcccg
     6961 tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat ccagcagatg
     7021 gtggtctgag aggatacact gacatcgcac tgaaagttga tggtggtggc catctgcact
     7081 gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg gaacatcaag atgcccggtg
     7141 tccatgccgt tgatcaccgc ctggaaagga tcgaggagag tgacaatgaa acctacgtag
     7201 tgcaaagaga agtggcagtt gccaaataca gcaaccttgg tggtggcatg gacgagctgt
     7261 acaagggatc caccggatct agataacgcc catcgataga tcctaatcaa cctctggatt
     7321 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     7381 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     7441 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     7501 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     7561 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     7621 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     7681 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     7741 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     7801 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     7861 cgagtcggat ctccctttgg gccgcctccc cgcctgagat cctttaagac caatgactta
     7921 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7981 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8041 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8101 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8161 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     8221 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggcccgggtt
     8281 aattaaggaa agggctagat cattcttgaa gacgaaaggg cctcgtgata cgcctatttt
     8341 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     8401 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     8461 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     8521 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     8581 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     8641 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     8701 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg
     8761 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     8821 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     8881 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     8941 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     9001 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     9061 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     9121 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     9181 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     9241 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     9301 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     9361 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     9421 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     9481 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     9541 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     9601 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     9661 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     9721 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     9781 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     9841 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     9901 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     9961 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
    10021 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
    10081 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
    10141 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
    10201 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
    10261 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
    10321 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagcaag ctcatggctg
    10381 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
    10441 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctccccg tggcacgaca
    10501 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    10561 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    10621 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaatttc acaaataaag
    10681 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
    10741 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
    10801 ctattaccac tgccaattac ctgtggtttc atttactcta aacctgtgat tcctctgaat
    10861 tattttcatt ttaaagaaat tgtatttgtt aaatatgtac tacaaactta gtagt
//
