LOCUS       Exported               10207 bp DNA     circular SYN 29-OCT-2020
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(2341..2360)
                     /label=qk-EF1a-R
                     /note="color: black; sequence: GAGCCAGTACACGACATCAC"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     3353..3373
                     /label=qk-PEF-F(EF-1a-F)
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     primer_bind     3385..3429
                     /label=fragment for
                     /note="color: black; sequence: 
                     cttccatttcaggtgtcgtgaGGTTTAGTGAACCGTCAGATCCGC"
     primer_bind     complement(3385..3429)
                     /label=vector rev
                     /note="color: black; sequence: 
                     GCGGATCTGACGGTTCACTAAACCtcacgacacctgaaatggaag"
     primer_bind     3407..3419
                     /label=pCEP-F
                     /note="color: black; sequence: AGAGCTCGTTTAGTGAACCG"
     primer_bind     3418..3469
                     /label=pmNeonGreen-F-donnor
                     /note="color: black; sequence: 
                     CGTCAGATCCGCTAGCGCTACCGGTCGCCACCatggtgtccaagggcgaaga"
     primer_bind     3440..3471
                     /label=28a_mneongreen_ddx21_dat_F_bamhi
                     /note="color: black; sequence: 
                     acagcaaatgggtcgcggatccatggtgtccaagggcgaagagg"
     primer_bind     3448..3471
                     /label=28a_mneongreen_ddx21_dat_F_bamhi
                     /note="color: black; sequence: 
                     acagcaaatgggtcgcggatccatggtgtccaagggcgaagagg"
     CDS             3450..4157
                     /label=mNeongreen
                     /note="modified_by=User"
                     /note="color: #993366; direction: RIGHT"
     primer_bind     complement(3450..3471)
                     /label=DDX21_NG_OVER_R_1
                     /note="color: black; sequence: 
                     cctcttcgcccttggacaccatTTGACCAAATGCTTTACTGAAA"
     primer_bind     complement(4138..4195)
                     /label=pmNeonGreen-R-donnor
                     /note="color: black; sequence: 
                     GCAGAATTCGAAGCTTGAGCTCGAGATCTGAGTCCGGActtgtacagctcgtccatgc"
     misc_feature    4172..4203
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     primer_bind     4187..4230
                     /label=DDX21-DAT-F-KPNI
                     /note="color: black; sequence: 
                     GAATTCTGCAGTCGACGGTACCCCGGGAAAACTCCGTAGTGACG"
     misc_feature    4189..4203
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     4191..4230
                     /label=DDX21_mneonG_F_NheI
                     /note="color: black; sequence: 
                     gccgcaccggtctgcagctagcATGCCGGGAAAACTCCGTAGTGACG"
     misc_feature    4209..6557
                     /label=DDX21
                     /note="color: #a6acb3"
     CDS             4209..6554
                     /codon_start=1
                     /label=DDX21
                     /note="color: #993366"
                     /translation="PGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAE
                     EEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKK
                     VVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPS
                     EAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSG
                     KDLIAQARTGTDATFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSD
                     ITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEV
                     DQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLI
                     GKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSA
                     IKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDV
                     ESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSK
                     DAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGF
                     VTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQE
                     KWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGP
                     RGQRSGGGNKSNRSQNKGQKRSFSKAFGQ"
     misc_feature    4209..6554
                     /label=DDX21-DAT
                     /note="color: #a6acb3"
     primer_bind     4209..4230
                     /label=DDX21_mneonG_F_NheI
                     /note="color: black; sequence: 
                     gccgcaccggtctgcagctagcATGCCGGGAAAACTCCGTAGTGACG"
     primer_bind     4814..4833
                     /label=Primer 1
                     /note="color: black; sequence: CCGAGGAGTGACCTTCCTAT"
     misc_feature    4854..5393
                     /label=DDX21-D1
                     /note="color: #a6acb3"
     primer_bind     4886..4929
                     /label=DDX21-GKT-DAT-F
                     /note="color: black; sequence: 
                     TGCACAGGCACGGACAGGAACTGACGCTACATTCTCCTTTGCCA"
     primer_bind     complement(4886..4929)
                     /label=DDX21-GKT-DAT-R
                     /note="color: black; sequence: 
                     TGGCAAAGGAGAATGTAGCGTCAGTTCCTGTCCGTGCCTGTGCA"
     misc_feature    4908..4913
                     /label=DAT-235-236
                     /note="color: #a6acb3"
     primer_bind     5067..5086
                     /label=DDX21-seq-F
                     /note="color: black; sequence: TCAGTGGCTTGTTTTTATGG"
     primer_bind     complement(5202..5244)
                     /label=E340A-R
                     /note="color: black; sequence: 
                     TATCCAACATCTGGTCCACGGCATCCAGGACAACATGCTTAAG"
     primer_bind     5204..5248
                     /label=E340A-F
                     /note="color: black; sequence: 
                     TAAGCATGTTGTCCTGGATGCCGTGGACCAGATGTTGGATATGGG"
     misc_feature    5394..5924
                     /label=DDX21-D2
                     /note="color: #a6acb3"
     primer_bind     complement(5986..6005)
                     /label=DDX21-seq-R2
                     /note="color: black; sequence: CTTCTCAGCTGATTGTTTGA"
     primer_bind     complement(6532..6563)
                     /label=DDX21-DAT-XbaI-R
                     /note="color: black; sequence: 
                     cgttaggggggggggagggatcctctagattaTTATTGACCAAATGCTTTACTGAAAC"
     primer_bind     complement(6532..6557)
                     /label=DDX21-DAT-XbaI-R
                     /note="color: black; sequence: 
                     cgttaggggggggggagggatcctctagattaTTATTGACCAAATGCTTTACTGAAAC"
     primer_bind     complement(6533..6579)
                     /label=DDX21-DAT-R-BAMHI
                     /note="color: black; sequence: 
                     gTTATCTAGATCCGGTGGATCCTTATTGACCAAATGCTTTACTGAAA"
     primer_bind     complement(6533..6554)
                     /label=DDX21_NG_OVER_R_1
                     /note="color: black; sequence: 
                     cctcttcgcccttggacaccatTTGACCAAATGCTTTACTGAAA"
     primer_bind     complement(6533..6554)
                     /label=28a_mneongreen_ddx21_dat_R_noti
                     /note="color: black; sequence: 
                     ggtggtgctcgagtgcggccgcTTGACCAAATGCTTTACTGAAA"
     primer_bind     complement(6533..6554)
                     /label=DDX21-Ruby3-R1-overlap
                     /note="color: black; sequence: 
                     gctcttcgcccttagacaccatTTGACCAAATGCTTTACTGAAA"
     misc_feature    6554
                     /label=????
                     /note="color: #a6acb3"
     primer_bind     6558..6603
                     /label=vector for
                     /note="color: black; sequence: 
                     GGATCCACCGGATCTAGATAAcgcccATcgatagatcctaatcaac"
     misc_feature    6559..6577
                     /label=Feature 21
                     /note="color: #a6acb3"
     misc_feature    6559..6563
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     CDS             6578
                     /codon_start=1
                     /label=PuroR
                     /note="color: #ccffcc"
                     /translation=""
     misc_feature    6597..7185
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     CDS             complement(7068..7079)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 7067"
                     /translation="IEGR"
     LTR             7260..7493
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7664..7683)
                     /label=qk-PBR322.F
                     /note="color: black; sequence: AAGTGCCACCTGACGTCTAA"
     promoter        7698..7802
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7803..8663
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7803 .. 7871 / #ccffcc / signal sequence
                        2: 7872 .. 8663 / #ccffcc
                     Cleavage site after base 7871"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8834..9422
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    9833..9854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     9863..9882
                     /label=qk-PCAMBIA1301-F
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGC"
     promoter        9869..9899
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9869 .. 9874 / #ffffff / -35
                        2: 9875 .. 9892 / #ffffff
                        3: 9893 .. 9899 / #ffffff / -10"
     protein_bind    9907..9923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9910..9929
                     /label=qk-pQE30-F
                     /note="color: black; sequence: TGAGCGGATAACAATTTCAC"
     primer_bind     9912..9935
                     /label=qk-M13R-48
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     9931..9947
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9997..10016
                     /label=qk-EBV-R
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     10005..10024
                     /label=qk-pCMV-R
                     /note="color: black; sequence: TCCAAACTCATCAATGTATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggttt agtgaaccgt
     3421 cagatccgct agcgctaccg gtcgccacca tggtgtccaa gggcgaagag gacaacatgg
     3481 ccagcctgcc tgccacccac gagctgcaca tcttcggcag catcaacggc gtggacttcg
     3541 acatggtggg acagggcacc ggcaacccca acgacggcta cgaggaactg aacctgaagt
     3601 ccacaaaggg cgacctgcag ttcagcccct ggattctggt gccccacatc ggctacggct
     3661 tccaccagta cctgccctac cccgacggca tgagcccttt tcaggccgcc atggtggatg
     3721 gcagcggcta ccaggtgcac cggaccatgc agtttgagga cggcgccagc ctgaccgtga
     3781 actaccggta cacatacgag ggcagccaca tcaagggcga ggcccaagtg aagggcacag
     3841 gctttccagc cgacggcccc gtgatgacca atagcctgac agccgccgac tggtgcagaa
     3901 gcaagaaaac ctaccccaat gacaagacca tcatcagcac cttcaagtgg tcctacacca
     3961 ccggcaatgg caagcggtac agaagcaccg cccggaccac ctacaccttc gccaaaccca
     4021 tggccgccaa ctacctgaag aaccagccta tgtacgtgtt ccgcaagacc gagctgaagc
     4081 actccaagac agaactgaac ttcaaagagt ggcagaaagc cttcaccgac gtgatgggca
     4141 tggacgagct gtacaagtcc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
     4201 acggtacccc gggaaaactc cgtagtgacg ctggtttgga atcagacacc gcaatgaaaa
     4261 aaggggagac actgcgaaag caaaccgagg agaaagagaa aaaagagaag ccaaaatctg
     4321 ataagactga agagatagca gaagaggaag aaactgtttt ccccaaagct aaacaagtta
     4381 aaaagaaagc agagccttct gaagttgaca tgaattctcc taaatccaaa aaggcaaaaa
     4441 agaaagagga gccatctcaa aatgacattt ctcctaaaac caaaagtttg agaaagaaaa
     4501 aggagcccat tgaaaagaaa gtggtttctt ctaaaaccaa aaaagtgaca aaaaatgagg
     4561 agccttctga ggaagaaata gatgctccta agcccaagaa gatgaagaaa gaaaaggaaa
     4621 tgaatggaga aactagagag aaaagcccca aactgaagaa tggatttcct catcctgaac
     4681 cggactgtaa ccccagtgaa gctgccagtg aagaaagtaa cagtgagata gagcaggaaa
     4741 tacctgtgga acaaaaagaa ggcgctttct ctaattttcc catatctgaa gaaactatta
     4801 aacttctcaa aggccgagga gtgaccttcc tatttcctat acaagcaaag acattccatc
     4861 atgtttacag cgggaaggac ttaattgcac aggcacggac aggaactgac gctacattct
     4921 cctttgccat ccctttgatt gagaaacttc atggggaact gcaagacagg aagagaggcc
     4981 gtgcccctca ggtactggtt cttgcaccta caagagagtt ggcaaatcaa gtaagcaaag
     5041 acttcagtga catcacaaaa aagctgtcag tggcttgttt ttatggtgga actccctatg
     5101 gaggtcaatt tgaacgcatg aggaatggga ttgatatcct ggttggaaca ccaggtcgta
     5161 tcaaagacca catacagaat ggcaaactag atctcaccaa acttaagcat gttgtcctgg
     5221 atgaagtgga ccagatgttg gatatgggat ttgctgatca agtggaagag attttaagtg
     5281 tggcatacaa gaaagattct gaagacaatc cccaaacatt gcttttttct gcaacttgcc
     5341 ctcattgggt atttaatgtt gccaagaaat acatgaaatc tacatatgaa caggtggacc
     5401 tgattggtaa aaagactcag aaaacggcaa taactgtgga gcatctggct attaagtgcc
     5461 actggactca gagggcagca gttattgggg atgtcatccg agtatatagt ggtcatcaag
     5521 gacgcactat catcttttgt gaaaccaaga aagaagccca ggagctgtcc cagaattcag
     5581 ctataaagca ggatgctcag tccttgcatg gagacattcc acagaagcaa agggaaatca
     5641 ccctgaaagg ttttagaaat ggtagttttg gagttttggt ggcaaccaat gttgctgcac
     5701 gtgggttaga catccctgag gttgatttgg ttatacaaag ctctccacca aaggatgtag
     5761 agtcctacat tcatcgatcc gggcggacag gcagagctgg aaggacgggg gtgtgcatct
     5821 gcttttatca gcacaaggaa gaatatcagt tagtacaagt ggagcaaaaa gcgggaatta
     5881 agttcaaacg aataggtgtt ccttctgcaa cagaaataat aaaagcttcc agcaaagatg
     5941 ccatcaggct tttggattcc gtgcctccca ctgccattag tcacttcaaa caatcagctg
     6001 agaagctgat agaggagaag ggagctgtgg aagctctggc agcagcactg gcccatattt
     6061 caggtgccac gtccgtagac cagcgctcct tgatcaactc aaatgtgggt tttgtgacca
     6121 tgatcttgca gtgctcaatt gaaatgccaa atattagtta tgcttggaaa gaacttaaag
     6181 agcagctggg cgaggagatt gattccaaag tgaagggaat ggtttttctc aaaggaaagc
     6241 tgggtgtttg ctttgatgta cctaccgcat cagtaacaga aatacaggag aaatggcatg
     6301 attcacgacg ctggcagctc tctgtggcca cagagcaacc agaactggaa ggaccacggg
     6361 aaggatatgg aggcttcagg ggacagcggg aaggcagtcg aggcttcagg ggacagcggg
     6421 acggaaacag aagattcaga ggacagcggg aaggcagtag aggcccgaga ggacagcgat
     6481 caggaggtgg caacaaaagt aacagatccc aaaacaaagg ccagaagcgg agtttcagta
     6541 aagcatttgg tcaataagga tccaccggat ctagataacg cccatcgata gatcctaatc
     6601 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6661 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6721 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6781 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6841 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6901 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6961 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7021 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7081 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7141 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag atcctttaag
     7201 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7261 ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct
     7321 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7381 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7441 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7501 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7561 gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag ggcctcgtga
     7621 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     7681 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     7741 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     7801 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     7861 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     7921 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     7981 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     8041 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     8101 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     8161 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     8221 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     8281 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     8341 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     8401 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     8461 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     8521 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     8581 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     8641 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     8701 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     8761 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     8821 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     8881 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     8941 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     9001 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     9061 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     9121 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     9181 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     9241 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     9301 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     9361 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     9421 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     9481 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     9541 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     9601 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagca
     9661 agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     9721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     9781 cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     9841 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     9901 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg attacgaatt
     9961 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10021 tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat cccaaacttc
    10081 ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc taaacctgtg
    10141 attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt actacaaact
    10201 tagtagt
//
