LOCUS       Exported                9974 bp DNA     circular SYN 17-OCT-2018
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   oucxuguang
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     2088..2112
                     /label=U6-pLL3.7
                     /note="color: black; sequence: cagtgcaggggaaagaatagtagac"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(2327..2346)
                     /label=pCDH-CMV.R
                     /note="color: black; sequence: catcactttcccagtttacc"
     primer_bind     complement(2341..2360)
                     /label=qk-EF1a-R
                     /note="color: black; sequence: GAGCCAGTACACGACATCAC"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     3353..3373
                     /label=qk-PEF-F(EF-1a-F)
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     primer_bind     3353..3373
                     /label=Primer 1
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     primer_bind     complement(3389..3410)
                     /label=p24-R-kpnI
                     /note="color: black; sequence: 
                     GGGGTACCgcttcacgacacctgaaatg"
     primer_bind     3409..3452
                     /label=p23-egfp-fbl-f-nhei
                     /note="color: black; sequence: 
                     ggccgcaccggtctgcagctagATGGTGAGCAAGGGCGAGGAGC"
     misc_feature    3409..3430
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     3412..3450
                     /label=ECFP-AgeI-F
                     /note="color: black; sequence: 
                     GCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGAGGA"
     CDS             3431..4147
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3431 .. 3433 / #05fd14
                        2: 3434 .. 3436 / #05fd14 / 1a
                        3: 3437 .. 4147 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3431..3452)
                     /label=?????
                     /note="color: black; sequence: 
                     GCTCCTCGCCCTTGCTCACCATTTTCAGGTGTCGTGAAGCGG"
     primer_bind     3431..3450
                     /label=ECFP-AgeI-F
                     /note="color: black; sequence: 
                     GCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGAGGA"
     primer_bind     complement(3476..3497)
                     /label=pEGFP-N-3
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     4084..4105
                     /label=pEGFP-C-5
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     complement(4126..4170)
                     /label=ECFP-XhoI-R
                     /note="color: black; sequence: 
                     TGAGCTCGAGATCTCTTGTACAGCTCGTCCATGCCG"
     misc_feature    4148..4170
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     primer_bind     4169..4197
                     /label=FBL-HindIII-F
                     /note="color: black; sequence: 
                     CCCAAGCTTCCATGAAGCCAGGATTCAGTCC"
     CDS             4178..5140
                     /codon_start=1
                     /label=Fibrillarin
                     /note="color: #993366"
                     /translation="MKPGFSPRGGGFGGRGGFGDRGGRGGRGGFGGGRGRGGGFRGRGR
                     GGGGGGGGGGGGGRGGGGFHSGGNRGRGRGGKRGNQSGKNVMVEPHRHEGVFICRGKED
                     ALVTKNLVPGESVYGEKRVSISEGDDKIEYRAWNPFRSKLAAAILGGVDQIHIKPGAKV
                     LYLGAASGTTVSHVSDIVGPDGLVYAVEFSHRSGRDLINLAKKRTNIIPVIEDARHPHK
                     YRMLIAMVDVIFADVAQPDQTRIVALNAHTFLRNGGHFVISIKANCIDSTASAEAVFAS
                     EVKKMQQENMKPQEQLTLEPYERDHAVVVGVYRPPPKVKN"
     primer_bind     4439..4461
                     /label=sgRNA1
                     /note="color: black; sequence: GTGGAGCCGCATCGGCATGAGGG"
     primer_bind     4445..4467
                     /label=sgrna3
                     /note="color: black; sequence: CCGCATCGGCATGAGGGTGTCTT"
     primer_bind     4457..4479
                     /label=sgrna2
                     /note="color: black; sequence: GAGGGTGTCTTCATTTGTCGAGG"
     primer_bind     complement(5119..5162)
                     /label=p23-egfp-fbl-r-xbai
                     /note="color: black; sequence: 
                     gggagaggggcagatccTCTAGGTTCTTCACCTTGGGGGGTGGC"
     primer_bind     complement(5121..5140)
                     /label=FBL-KpnI-R
                     /note="color: black; sequence: 
                     CGGGGTACCGTTCTTCACCTTGGGGGGTG"
     primer_bind     5141..5165
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GCTCTAGAggatctgcccctctccctcc"
     misc_feature    5141..5162
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     5141..5161
                     /label=dCas9-puro-F(NotI)
                     /note="color: black; sequence: 
                     ATAAGAATGCGGCCGCTAGCTAGAggatctgcccctctcc"
     misc_feature    5153..5726
                     /label=IRES
                     /note="color: #a6acb3"
     primer_bind     complement(5174..5197)
                     /label=PQC-3primer
                     /note="color: black; sequence: AAGCGGCTTCGGCCAGTAACGTTA"
     primer_bind     complement(5199..5218)
                     /label=qk-pIRES2-EGFP.P3
                     /note="color: black; sequence: AACGCACACCGGCCTTATTC"
     primer_bind     complement(5199..5218)
                     /label=pIRES2-EGFP.P3
                     /note="color: black; sequence: AACGCACACCGGCCTTATTC"
     primer_bind     complement(5202..5221)
                     /label=p23-detcet-reverse
                     /note="color: black; sequence: acaaacgcacaccggcctta"
     primer_bind     complement(5248..5267)
                     /label=qk-IRES-R
                     /note="color: black; sequence: CCTCACATTGCCAAAAGACG"
     primer_bind     complement(5248..5267)
                     /label=IRES-R
                     /note="color: black; sequence: CCTCACATTGCCAAAAGACG"
     primer_bind     5547..5566
                     /label=qk-IRES-F
                     /note="color: black; sequence: TGGCTCTCCTCAAGCGTATT"
     CDS             5746..6345
                     /codon_start=1
                     /label=PuroR
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5982..5993)
                     /label=qk-ACYCDuetUP1
                     /note="color: black; sequence: GGATCTCGACGCTCTCC"
     primer_bind     complement(6326..6346)
                     /label=dCas9-puro-R(XhoI)
                     /note="color: black; sequence: 
                     CCGCTCGAGgtcaggcaccgggcttgcggg"
     primer_bind     6361..6380
                     /label=p24-F-XbaI
                     /note="color: black; sequence: 
                     GCTCTAGAcctaatcaacctctggatta"
     misc_feature    6364..6952
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(6444..6463)
                     /label=WPRE-r
                     /note="color: black; sequence: ACAAAGGCATTAAAGCAGCG"
     CDS             complement(6835..6846)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 6834"
                     /translation="IEGR"
     LTR             7027..7260
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(7431..7450)
                     /label=qk-PBR322.F
                     /note="color: black; sequence: AAGTGCCACCTGACGTCTAA"
     promoter        7465..7569
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7570..8430
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7570 .. 7638 / #ccffcc / signal sequence
                        2: 7639 .. 8430 / #ccffcc
                     Cleavage site after base 7638"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8601..9189
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(9490..9508)
                     /label=PBABE-R
                     /note="color: black; sequence: CCTCACTACTTCTGGAATA"
     protein_bind    9600..9621
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     9606..9626
                     /label=3.0rev
                     /note="color: black; sequence: GAGTTAGCTCACTCATTAGGC"
     primer_bind     9630..9649
                     /label=qk-PCAMBIA1301-F
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGC"
     primer_bind     9630..9649
                     /label=P1
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGC"
     primer_bind     9633..9652
                     /label=PBI 101.F
                     /note="color: black; sequence: ggctttacactttatgcttc"
     primer_bind     9633..9652
                     /label=pTarget.R
                     /note="color: black; sequence: GGCTTTACACTTTATGCTTC"
     promoter        9636..9666
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9636 .. 9641 / #ffffff / -35
                        2: 9642 .. 9659 / #ffffff
                        3: 9660 .. 9666 / #ffffff / -10"
     protein_bind    9674..9690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9677..9696
                     /label=qk-pQE30-F
                     /note="color: black; sequence: TGAGCGGATAACAATTTCAC"
     primer_bind     9677..9696
                     /label=pQE30-F
                     /note="color: black; sequence: TGAGCGGATAACAATTTCAC"
     primer_bind     9679..9702
                     /label=qk-M13R-48
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     9679..9702
                     /label=M13R(-48)
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     9698..9714
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     9749..9768
                     /label=pIRES-R
                     /note="color: black; sequence: CACTGCATTCTAGTTGTGGT"
     primer_bind     9764..9783
                     /label=qk-EBV-R
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     9764..9783
                     /label=EBVrev
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     9772..9791
                     /label=qk-pCMV-R
                     /note="color: black; sequence: TCCAAACTCATCAATGTATC"
     primer_bind     9772..9791
                     /label=pCMV-R
                     /note="color: black; sequence: TCCAAACTCATCAATGTATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3481 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3541 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3601 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     3661 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3721 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3781 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3841 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3901 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3961 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4021 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4081 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4141 gtacaagtcc ggactcagat ctcgagctca agcttccatg aagccaggat tcagtccccg
     4201 tgggggtggc tttggcggcc gagggggctt tggtgaccgt ggtggtcgtg gaggccgagg
     4261 gggctttggc gggggccgag gtcgaggcgg aggctttaga ggtcgtggac gaggaggagg
     4321 tggaggcggc ggcggcggtg gaggaggagg aagaggtggt ggaggcttcc attctggtgg
     4381 caaccggggt cgtggtcggg gaggaaaaag aggaaaccag tcggggaaga atgtgatggt
     4441 ggagccgcat cggcatgagg gtgtcttcat ttgtcgagga aaggaagatg cactggtcac
     4501 caagaacctg gtccctgggg aatcagttta tggagagaag agagtctcga tttcggaagg
     4561 agatgacaaa attgagtacc gagcctggaa ccccttccgc tccaagctag cagcagcaat
     4621 cctgggtggt gtggaccaga tccacatcaa accgggggct aaggttctct acctcggggc
     4681 tgcctcgggc accacggtct cccatgtctc tgacatcgtt ggtccggatg gtctagtcta
     4741 tgcagtcgag ttctcccacc gctctggccg tgacctcatt aacttggcca agaagaggac
     4801 caacatcatt cctgtgatcg aggatgctcg acacccacac aaataccgca tgctcatcgc
     4861 aatggtggat gtgatctttg ctgatgtggc ccagccagac cagacccgga ttgtggccct
     4921 gaatgcccac accttcctgc gtaatggagg acactttgtg atttccatta aggccaactg
     4981 cattgactcc acagcctcag ccgaggccgt gtttgcctcc gaagtgaaaa agatgcaaca
     5041 ggagaacatg aagccgcagg agcagttgac ccttgagcca tatgaaagag accatgccgt
     5101 ggtcgtggga gtgtacaggc caccccccaa ggtgaagaac ctagaggatc tgcccctctc
     5161 cctccccccc ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg
     5221 tctatatgtt attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg
     5281 gccctgtctt cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag
     5341 gtctgttgaa tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt
     5401 ctgtagcgac cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc
     5461 aaaagccacg tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga
     5521 gttggatagt tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga
     5581 aggatgccca gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct
     5641 ttacatgtgt ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg
     5701 ttttcctttg aaaaacacga tgataaggat ccaccggagc ttaccatgac cgagtacaag
     5761 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     5821 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     5881 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5941 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6001 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6061 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6121 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6181 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6241 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6301 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc atcgatagat
     6361 cctaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     6421 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     6481 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     6541 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     6601 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     6661 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     6721 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     6781 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     6841 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     6901 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgagatc
     6961 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7021 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     7081 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7141 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7201 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7261 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7321 agagtgagag gcccgggtta attaaggaaa gggctagatc attcttgaag acgaaagggc
     7381 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     7441 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7501 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7561 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7621 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7681 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7741 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7801 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7861 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7921 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7981 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     8041 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     8101 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     8161 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     8221 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8281 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8341 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8401 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8461 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8521 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8581 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8641 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8701 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8761 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8821 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8881 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8941 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     9001 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     9061 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     9121 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     9181 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9241 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9301 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9361 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9421 tgcagcaagc tcatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     9481 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     9541 agctccccgt ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9601 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9661 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt
     9721 acgaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9781 atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc
     9841 aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa
     9901 acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact
     9961 acaaacttag tagt
//
