LOCUS       Exported               14189 bp DNA     circular SYN 21-OCT-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14189)
  AUTHORS   Triple Threat
  TITLE     Direct Submission
  JOURNAL   Exported Nov 16, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14189
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     492..511
                     /label=bs_PCDNA3.0-F2
                     /note="color: black; sequence: CTCTGGCTAACTAGAGAACC"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     2088..2112
                     /label=bs_U6.PLL3.7
                     /note="color: black; sequence: CAGTGCAGGGGAAAGAATAGTAGAC"
     primer_bind     2088..2112
                     /label=bs_SSN
                     /note="color: black; sequence: CAGTGCAGGGGAAAGAATAGTAGAC"
     primer_bind     2088..2112
                     /label=U6-pLL3.7
                     /note="color: black; sequence: cagtgcaggggaaagaatagtagac"
     primer_bind     complement(2089..2112)
                     /label=bs_MU6-C
                     /note="color: black; sequence: GTCTACTATTCTTTCCCCTGCACT"
     primer_bind     2091..2112
                     /label=bs_MU6-F
                     /note="color: black; sequence: TGCAGGGGAAAGAATAGTAGAC"
     primer_bind     complement(2194..2211)
                     /label=bs_U6-R
                     /note="color: black; sequence: CGCCAAAGTGGATCTCTGCT"
     primer_bind     2223..2240
                     /label=bs_EF1a?
                     /note="color: black; sequence: CGGCTCCGGTGCCCGTCAG"
     promoter        2224..3405
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     complement(2327..2346)
                     /label=pCDH-CMV.R
                     /note="color: black; sequence: catcactttcccagtttacc"
     primer_bind     complement(2341..2360)
                     /label=qk-EF1a-R
                     /note="color: black; sequence: GAGCCAGTACACGACATCAC"
     intron          2454..3396
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
                     /note="color: #------"
     primer_bind     2668..2689
                     /label=bs_PCDNA3-R
                     /note="color: black; sequence: ACAGTGGGAGTGGCACCTTC"
     primer_bind     2679..2689
                     /label=bs_PCDNA3-R
                     /note="color: black; sequence: ACAGTGGGAGTGGCACCTTC"
     primer_bind     3280..3300
                     /label=bs_EF1a-F:
                     /note="color: black; sequence: GTTAGGCCAGCTTGGCACTTG"
     primer_bind     3352..3373
                     /label=bs_fEF1a-F
                     /note="color: black; sequence: CTCAAGCCTCAGACAGTGGTTC"
     primer_bind     3353..3373
                     /label=qk-PEF-F(EF-1a-F)
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     primer_bind     3353..3373
                     /label=Primer 1
                     /note="color: black; sequence: TCAAGCCTCAGACAGTGGTTC"
     primer_bind     complement(3389..3410)
                     /label=p24-R-kpnI
                     /note="color: black; sequence: 
                     GGGGTACCgcttcacgacacctgaaatg"
     primer_bind     3408..3451
                     /label=P23_puro_egfp_rpa49_nhei_f
                     /note="color: black; sequence: 
                     cggccgcaccggtctgcagctagcATGGTGAGCAAGGGCGAGGA"
     misc_feature    3408..3430
                     /label=Feature 19
                     /note="color: #a6acb3"
     CDS             3432..4148
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 3432 .. 3434 / #05fd14
                        2: 3435 .. 3437 / #05fd14 / 1a
                        3: 3438 .. 4148 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3432..3453)
                     /label=?????
                     /note="color: black; sequence: 
                     GCTCCTCGCCCTTGCTCACCATTTTCAGGTGTCGTGAAGCGG"
     primer_bind     complement(3477..3498)
                     /label=pEGFP-N-3
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     3947..3959
                     
                     /note="color: black; sequence: 
                     AAAGCTTCCGGAGGTCCCGAGGGCGGCAGC"
     primer_bind     3973..3992
                     /label=pAcGFP1-C1.F
                     /note="color: black; sequence: ACCACTACCAGCAGAATACC"
     primer_bind     4038..4058
                     /label=bs_EGFP-C
                     /note="color: black; sequence: AGCACCCAGTCCGCCCTGAGC"
     primer_bind     4085..4106
                     /label=pEGFP-C-5
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     complement(4127..4171)
                     /label=ECFP-XhoI-R
                     /note="color: black; sequence: 
                     TGAGCTCGAGATCTCTTGTACAGCTCGTCCATGCCG"
     misc_feature    4149..4177
                     /label=MCS
                     /note="multiple cloning site"
                     /note="color: #99ccff"
     primer_bind     complement(4164..4216)
                     /label=28a_Pol1A_HindIII_Don_R
                     /note="color: black; sequence: 
                     ggtgctcgagtgcggccgcaagcttCTATCTCAGAGGCTGCTTGAGCTCG"
     primer_bind     complement(4164..4174)
                     /label=28a_Pol1A_HindIII_Don_R
                     /note="color: black; sequence: 
                     ggtgctcgagtgcggccgcaagcttCTATCTCAGAGGCTGCTTGAGCTCG"
     primer_bind     4170..4198
                     /label=POLR1A-HindIII-F
                     /note="color: black; sequence: 
                     CCCAAGCTTCCATGTTGATCTCCAAGAACAT"
     CDS             4179..9338
                     /codon_start=1
                     /label=POLR1A
                     /note="color: #993366"
                     /translation="MLISKNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNP
                     SANGLYDLALGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSC
                     LNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSASEIREELEQ
                     YTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKRCPHCKTGRSVVRKEHNSK
                     LTITFPAMVHRTAGQKDSEPLGIEEAQIGKRGYLTPTSAREHLSALWKNEGFFLNYLFS
                     GMDDDGMESRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVLIRK
                     LLALMAQEQKLPEEVATPTTDEEKDSLIAIDRSFLSTLPGQSLIDKLYNIWIRLQSHVN
                     IVFDSEMDKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGI
                     PMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDMTQREA
                     VAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKVLR
                     LHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDGQPLAGLIQDHM
                     VSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLIN
                     IIPEDHIPLNLSGKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAH
                     YGSSAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKR
                     QRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNH
                     YSNEINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGRRPPLMA
                     SGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREGLVDTAVKTSRSGYLQ
                     RCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIM
                     KSQHLHEVLSRADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLES
                     ENRNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETK
                     VDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQ
                     MTLNTFHFAGRGEMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQ
                     LTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPEDILRFME
                     TRFFKLLMESIKKKNNKASAFRNVNTRRATQRDLDNAGELGRSRGEQEGDEEEEGHIVD
                     AEAEEGDADASDAKRKEKQEEEVDYESEEEEEREGEENDDEDMQEERNPHREGARKTQE
                     QDEEVGLGTEEDPSLPALLTQPRKPTHSQEPQGPEAMERRVQAVREIHPFIDDYQYDTE
                     ESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGITRCLLNETTNNKNEKELVLN
                     TEGINLPELFKYAEVLDLRRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDP
                     RHLSLVADYMCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPS
                     ACLVVGKVVRGGTGLFELKQPLR"
     primer_bind     4182..4203
                     /label=28a_Pol1A_EcoRI_Don_F
                     /note="color: black; sequence: 
                     gcaaatgggtcgcggatccgaattcTTGATCTCCAAGAACATGCCCT"
     primer_bind     5142..5164
                     /label=POLR1A-2-F
                     /note="color: black; sequence: AATGGCCAGACGGTGAACTTGCA"
     primer_bind     complement(5186..5208)
                     /label=POLR1A-1-R
                     /note="color: black; sequence: ATGCCAGAAGTTTTCGAATCAGA"
     primer_bind     5676..5687
                     
                     /note="color: black; sequence: 
                     AAAGCTTCCGGAGGTCCCGAGGGCGGCAGC"
     primer_bind     6124..6146
                     /label=POLR1A-3-F
                     /note="color: black; sequence: CCCGGGAGCACTATATGGAGCTG"
     primer_bind     complement(6187..6209)
                     /label=POLR1A-2-R
                     /note="color: black; sequence: CTTCAGGATGGAAGGAGAAAGGA"
     primer_bind     7185..7207
                     /label=POLR1A-4-F
                     /note="color: black; sequence: TATGATCTCACGGTCCGTGACAG"
     primer_bind     complement(7239..7261)
                     /label=POLR1A-3-R
                     /note="color: black; sequence: TTGGGGATGTCCAGGCCATCCTC"
     primer_bind     8182..8204
                     /label=POLR1A-5-F
                     /note="color: black; sequence: CCGAGGACATCCTGCGCTTCATG"
     primer_bind     complement(8304..8326)
                     /label=POLR1A-4-R
                     /note="color: black; sequence: AACTCCCCAGCGTTGTCCAGATC"
     primer_bind     8485..8494
                     /label=P23_puro_egfp_rpa49_nhei_f
                     /note="color: black; sequence: 
                     cggccgcaccggtctgcagctagcATGGTGAGCAAGGGCGAGGA"
     primer_bind     8485..8494
                     /label=ECFP-AgeI-F
                     /note="color: black; sequence: 
                     GCGCTACCGGTCGCCACCATGGTGAGCAAGGGCGAGGA"
     primer_bind     complement(9317..9354)
                     /label=28a_Pol1A_HindIII_Don_R
                     /note="color: black; sequence: 
                     ggtgctcgagtgcggccgcaagcttCTATCTCAGAGGCTGCTTGAGCTCG"
     primer_bind     complement(9317..9349)
                     /label=28a_Pol1A_HindIII_Don_R
                     /note="color: black; sequence: 
                     ggtgctcgagtgcggccgcaagcttCTATCTCAGAGGCTGCTTGAGCTCG"
     primer_bind     complement(9319..9338)
                     /label=POLR1A-KpnI-R
                     /note="color: black; sequence: 
                     CGGGGTACCTCTCAGAGGCTGCTTGAGCT"
     primer_bind     9326..9376
                     /label=dCas9-puro-F(NotI)
                     /note="color: black; sequence: 
                     ATAAGAATGCGGCCGCTAGCTAGAggatctgcccctctcc"
     primer_bind     9355..9380
                     /label=Primer 1
                     /note="color: black; sequence: 
                     GCTCTAGAggatctgcccctctccctcc"
     misc_feature    9356..9378
                     /label=Feature 20
                     /note="color: #a6acb3"
     primer_bind     9356..9376
                     /label=dCas9-puro-F(NotI)
                     /note="color: black; sequence: 
                     ATAAGAATGCGGCCGCTAGCTAGAggatctgcccctctcc"
     misc_feature    9368..9941
                     /label=IRES
                     /note="color: #a6acb3"
     primer_bind     complement(9389..9412)
                     /label=PQC-3primer
                     /note="color: black; sequence: AAGCGGCTTCGGCCAGTAACGTTA"
     primer_bind     complement(9414..9433)
                     /label=qk-pIRES2-EGFP.P3
                     /note="color: black; sequence: AACGCACACCGGCCTTATTC"
     primer_bind     complement(9414..9433)
                     /label=pIRES2-EGFP.P3
                     /note="color: black; sequence: AACGCACACCGGCCTTATTC"
     primer_bind     complement(9417..9436)
                     /label=p23-detcet-reverse
                     /note="color: black; sequence: acaaacgcacaccggcctta"
     primer_bind     complement(9423..9441)
                     /label=bs_PIRES2-EGFP-R
                     /note="color: black; sequence: TATAGACAAACGCACACCG"
     primer_bind     complement(9451..9472)
                     /label=bs_pLVX-R
                     /note="color: black; sequence: CCAAAAGACGGCAATATGGTGG"
     primer_bind     complement(9453..9472)
                     /label=bs_PIZG1R (bp2947C)
                     /note="color: black; sequence: CCAAAAGACGGCAATATGGT"
     primer_bind     complement(9463..9482)
                     /label=qk-IRES-R
                     /note="color: black; sequence: CCTCACATTGCCAAAAGACG"
     primer_bind     complement(9463..9482)
                     /label=IRES-R
                     /note="color: black; sequence: CCTCACATTGCCAAAAGACG"
     primer_bind     9762..9781
                     /label=qk-IRES-F
                     /note="color: black; sequence: TGGCTCTCCTCAAGCGTATT"
     primer_bind     9840..9859
                     /label=bs_IRES-for
                     /note="color: black; sequence: CCTCGGTGCACATGCTTTAC"
     CDS             9961..10560
                     /codon_start=1
                     /label=PuroR
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10197..10208)
                     /label=qk-ACYCDuetUP1
                     /note="color: black; sequence: GGATCTCGACGCTCTCC"
     primer_bind     complement(10541..10561)
                     /label=dCas9-puro-R(XhoI)
                     /note="color: black; sequence: 
                     CCGCTCGAGgtcaggcaccgggcttgcggg"
     primer_bind     10576..10595
                     /label=p24-F-XbaI
                     /note="color: black; sequence: 
                     GCTCTAGAcctaatcaacctctggatta"
     misc_feature    10579..11167
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(10614..10636)
                     /label=bs_PCDNA3.4-R
                     /note="color: black; sequence: CAACATAGTTAAGAATACCAGTC"
     primer_bind     complement(10659..10678)
                     /label=WPRE-r
                     /note="color: black; sequence: ACAAAGGCATTAAAGCAGCG"
     primer_bind     10934..10946
                     /label=bs_CMV-24
                     /note="color: black; sequence: TATTAGGACAAGGCTGGTGGGCAC"
     CDS             complement(11050..11061)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2"
                     /translation="IEGR"
     LTR             11242..11475
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     11333..11352
                     /label=bs_PCDNA3.0-F2
                     /note="color: black; sequence: CTCTGGCTAACTAGAGAACC"
     primer_bind     complement(11646..11665)
                     /label=qk-PBR322.F
                     /note="color: black; sequence: AAGTGCCACCTGACGTCTAA"
     promoter        11680..11784
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             11785..12645
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11785 .. 11853 / #ccffcc / signal sequence
                        2: 11854 .. 12645 / #ccffcc"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      12816..13404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     13667..13686
                     /label=bs_SV40pro_F
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(13690..13701)
                     /label=bs_SV40pro_F
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     primer_bind     complement(13705..13723)
                     /label=PBABE-R
                     /note="color: black; sequence: CCTCACTACTTCTGGAATA"
     protein_bind    13815..13836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     13821..13841
                     /label=3.0rev
                     /note="color: black; sequence: GAGTTAGCTCACTCATTAGGC"
     primer_bind     13845..13864
                     /label=qk-PCAMBIA1301-F
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGC"
     primer_bind     13845..13864
                     /label=P1
                     /note="color: black; sequence: CCAGGCTTTACACTTTATGC"
     primer_bind     13848..13867
                     /label=PBI 101.F
                     /note="color: black; sequence: ggctttacactttatgcttc"
     primer_bind     13848..13867
                     /label=pTarget.R
                     /note="color: black; sequence: GGCTTTACACTTTATGCTTC"
     promoter        13851..13881
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 13851 .. 13856 / #ffffff / -35
                        2: 13857 .. 13874 / #ffffff
                        3: 13875 .. 13881 / #ffffff / -10"
     primer_bind     13859..13878
                     /label=bs_Lac-F
                     /note="color: black; sequence: TTATGCTTCCGGCTCGTATG"
     primer_bind     13885..13903
                     /label=bs_PETT7
                     /note="color: black; sequence: GGGAATTGTGAGCGGATAAC"
     protein_bind    13889..13905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     13892..13911
                     /label=qk-pQE30-F
                     /note="color: black; sequence: TGAGCGGATAACAATTTCAC"
     primer_bind     13892..13911
                     /label=pQE30-F
                     /note="color: black; sequence: TGAGCGGATAACAATTTCAC"
     primer_bind     13894..13917
                     /label=qk-M13R-48
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     13894..13917
                     /label=M13R(-48)
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGGA"
     primer_bind     13894..13915
                     /label=bs_PQE30-F
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAG"
     primer_bind     13897..13916
                     /label=bs_PBI101-F
                     /note="color: black; sequence: GGATAACAATTTCACACAGG"
     primer_bind     13913..13929
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     primer_bind     13964..13983
                     /label=pIRES-R
                     /note="color: black; sequence: CACTGCATTCTAGTTGTGGT"
     primer_bind     13964..13981
                     /label=bs_PSV40 (2)
                     /note="color: black; sequence: CACTGCATTCTAGTTGTG"
     primer_bind     13964..13981
                     /label=bs_PSV40
                     /note="color: black; sequence: CACTGCATTCTAGTTGTG"
     primer_bind     complement(13965..13985)
                     /label=bs_SV40
                     /note="color: black; sequence: AAACCACAACTAGAATGCAGT"
     primer_bind     complement(13965..13985)
                     /label=bs_PSG5-R(SV40)
                     /note="color: black; sequence: AAACCACAACTAGAATGCAGT"
     primer_bind     13971..13990
                     /label=bs_PCMV-myc-R-bd
                     /note="color: black; sequence: TTCTAGTTGTGGTTTGTCCA"
     primer_bind     13972..13989
                     /label=bs_PCMV-HA-R
                     /note="color: black; sequence: CAATCTAGTTGTGGTTTGTCC"
     primer_bind     complement(13974..13993)
                     /label=bs_pSG5-R
                     /note="color: black; sequence: GTTTGGACAAACCACAACTA"
     primer_bind     13979..13998
                     /label=qk-EBV-R
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     13979..13998
                     /label=EBVrev
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     13982..14003
                     /label=bs_PmirGLO-R
                     /note="color: black; sequence: GTTTGTCCAAACTCATCAATGT"
     primer_bind     13987..14006
                     /label=qk-pCMV-R
                     /note="color: black; sequence: TCCAAACTCATCAATGTATC"
     primer_bind     13987..14006
                     /label=pCMV-R
                     /note="color: black; sequence: TCCAAACTCATCAATGTATC"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtcgt
     2221 gaggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg
     2281 ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa
     2341 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg
     2401 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg
     2461 ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa
     2521 ttacttccac gcccctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     2581 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     2641 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     2701 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     2761 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     2821 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     2881 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     2941 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     3001 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     3061 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     3121 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     3181 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     3241 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     3301 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     3361 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaagcgg ccgcaccggt
     3421 ctgcagctag catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3481 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3541 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3601 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3661 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3721 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3781 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3841 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     3901 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     3961 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4021 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4081 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4141 tgtacaagtc cggactcaga tctcgagctc aagcttccat gttgatctcc aagaacatgc
     4201 cctggcggcg gctgcagggc atttccttcg ggatgtattc ggctgaagag ctcaagaaat
     4261 taagtgttaa atccattacg aaccctcgat acctggacag cctggggaac ccatcggcaa
     4321 acggcctgta cgatttagct ttgggccctg cagattccaa agaggtgtgc tccacctgcg
     4381 tgcaggactt cagcaactgt tctgggcacc tgggccacat tgagctccca ctcacagtgt
     4441 ataaccctct cctcttcgat aagctgtacc tgctgcttcg gggctcttgt ttaaactgcc
     4501 acatgctgac ttgtccccgg gccgtgattc acctcttact ctgccagctg agggttctgg
     4561 aagtcggggc cctacaagca gtctacgagc ttgagagaat tctgaacagg tttctggaag
     4621 aaaatcccga tccctctgcc tctgaaattc gggaggaatt agaacaatac acaactgaaa
     4681 ttgtgcagaa caacctcctg gggtcccagg gcgcacatgt aaagaacgtg tgtgagagca
     4741 agagcaagct cattgctctc ttctggaagg cacatatgaa tgctaagcgc tgtccccact
     4801 gcaagaccgg gcgatccgtt gtccgaaagg aacacaacag caagttgact atcacgtttc
     4861 cagccatggt gcacaggaca gctggccaga aggactctga gcccctggga attgaggaag
     4921 ctcagatagg aaaacgagga tacttaacac ccaccagtgc ccgcgaacac ctttctgccc
     4981 tgtggaagaa tgaaggattc tttctgaact accttttttc gggaatggat gatgatggta
     5041 tggaatccag attcaatccc agtgtgttct ttctagattt cttggtggtg ccgccctcaa
     5101 ggtatcgccc agtcagtcgc ctaggagacc agatgtttac taatggccag acggtgaact
     5161 tgcaggctgt catgaaggat gtagttctga ttcgaaaact tctggcattg atggcccaag
     5221 aacagaagtt gccagaggaa gtggccacac ccactacaga tgaggaaaaa gactctttga
     5281 ttgctattga ccgatccttt ttgagtacac ttccaggcca gtccctcata gacaaacttt
     5341 acaacatttg gattcgcctt cagagccacg tcaatattgt gtttgatagc gagatggaca
     5401 aactaatgat ggacaagtac ccaggcatta ggcagatcct ggagaagaaa gaaggcctgt
     5461 tccgaaaaca catgatggga aagcgagtgg actacgctgc gcgctcagtc atctgcccag
     5521 acatgtacat caacaccaac gaaattggaa ttcccatggt gtttgccaca aaactgacct
     5581 acccacagcc agttacccca tggaatgttc aggaacttag gcaagcggtc atcaacggcc
     5641 ctaatgtgca cccaggagcc tccatggtca tcaatgagga cggcagccgc acagccctga
     5701 gcgctgtgga catgacccag cgagaggccg tggccaagca gcttctgacc ccagccacgg
     5761 gggcacctaa gccccagggg acaaaaattg tgtgccggca tgtgaagaat ggggacattc
     5821 tgctactgaa ccgacagccc acactgcaca gaccctccat ccaggcccac cgtgcccgca
     5881 tcctgcctga agagaaagtg ctgcggctcc actatgccaa ctgcaaggcc tataatgccg
     5941 actttgatgg agacgagatg aatgcccatt tcccccagag tgagctgggc cgggccgagg
     6001 cctacgtcct ggcctgcact gatcagcagt accttgttcc caaggatggc caaccattgg
     6061 cgggactgat ccaggatcac atggtttcag gggcaagcat gactactcgg ggttgctttt
     6121 tcacccggga gcactatatg gagctggtgt accgaggact cacggacaaa gtggggcgcg
     6181 tgaagctcct ttctccttcc atcctgaagc cctttccgct gtggacagga aaacaggttg
     6241 tgtcaacgct gctcataaat ataatcccag aggaccacat cccactgaac ttatctggaa
     6301 aggcgaaaat cactgggaaa gcctgggtga aggaaactcc tcgatccgtt cctggcttta
     6361 accctgactc gatgtgcgag tcccaggtga tcatcaggga aggggagctg ctctgcggag
     6421 tgctggacaa ggcgcactat gggagctccg cctacggcct ggtccactgc tgctatgaga
     6481 tctatggagg cgagaccagc ggcaaggttc taacctgcct ggcccgcctc ttcaccgcct
     6541 acctgcagct ctacagaggc ttcaccttgg gcgtggaaga cattttggtg aagccaaagg
     6601 cagatgtcaa gaggcaacgt atcattgaag aatccaccca ctgcgggccc caggctgtca
     6661 gggctgcatt aaacctgcca gaagccgcat catatgatga ggtccgagga aaatggcagg
     6721 atgcccatct gggcaaggac cagagggatt ttaacatgat tgatctgaag ttcaaggagg
     6781 aagtgaacca ttacagcaat gagattaaca aggcatgcat gccttttggc ctacacagac
     6841 agttcccaga gaacagcctg cagatgatgg tgcagtcggg agccaaaggt tcaactgtga
     6901 acacgatgca gatctcgtgc ctgctgggcc agattgaact ggaaggtcgg agacccccgc
     6961 tgatggcgtc tggcaagtca ctgccctgct ttgagcctta tgagttcacc cccagggctg
     7021 gtggctttgt cactggcagg ttcctcaccg gcatcaaacc tcctgagttc ttcttccact
     7081 gcatggcagg acgagagggc ctggtggaca ctgctgtgaa aaccagccgc tcaggctatc
     7141 tccaaaggtg catcatcaag cacctagagg ggctggtcgt gcagtatgat ctcacggtcc
     7201 gtgacagtga cggcagtgtg gtgcagttcc tgtatgggga ggatggcctg gacatcccca
     7261 agacacagtt cctgcagccc aagcagttcc ccttcctggc cagcaactac gaggtgataa
     7321 tgaaatcaca gcatctccat gaagttttat ccagagcaga tcccaaaaaa gctctccacc
     7381 acttcagagc tatcaaaaaa tggcaaagca agcaccccaa caccctgctg agaagaggcg
     7441 ccttcttgag ttattcccag aaaattcagg aagctgtgaa agccctgaaa cttgagagtg
     7501 aaaaccgcaa tggccgcagc cctgggactc aggagatgct gaggatgtgg tatgagttgg
     7561 atgaggaaag ccgaaggaaa taccagaaga aggcggccgc ttgtcctgac cccagtctgt
     7621 ctgtctggcg tcctgacatc tactttgcat cagtgtcaga aacatttgaa acaaaggttg
     7681 atgactacag tcaagagtgg gcagctcaaa cagagaagag ttatgagaaa tcagagcttt
     7741 ctctcgacag gttgaggacc ttgctgcagc tgaagtggca gcgctcactg tgtgagccgg
     7801 gcgaggctgt gggcctgctg gctgcccaga gcatcggaga gccctccacc cagatgaccc
     7861 tcaacacctt ccactttgca ggcagaggcg agatgaacgt caccctgggc attccaaggt
     7921 tgcgggagat tctcatggtg gccagcgcca acatcaagac acccatgatg agcgtgcccg
     7981 tgctcaacac caagaaagcc ctgaagagag tgaaaagcct gaagaagcaa ctcaccaggg
     8041 tgtgcttggg ggaggtgttg cagaaaattg acgtccagga gtccttctgt atggaagaaa
     8101 aacagaacaa attccaggtg taccagctgc ggtttcagtt cctgccacat gcatattacc
     8161 agcaggagaa gtgcctgaga cccgaggaca tcctgcgctt catggaaaca agattcttta
     8221 aacttctgat ggaatccatc aaaaagaaga ataataaagc atcagctttc aggaacgtaa
     8281 acactcgaag agctacacag cgggatctgg acaacgctgg ggagttgggg aggagtcggg
     8341 gagagcagga gggtgatgag gaagaggagg ggcacattgt ggatgctgaa gctgaggagg
     8401 gggacgccga tgcctctgat gccaaacgca aggagaagca ggaggaggag gttgattatg
     8461 agagtgagga agaggaggag agggagggcg aggagaacga cgatgaagac atgcaggagg
     8521 aacgaaatcc ccacagggaa ggtgctcgaa agacccaaga gcaagatgaa gaggtgggct
     8581 taggcactga ggaggacccg tcccttcccg ccctcctgac gcagccccgg aaacccaccc
     8641 acagccagga gccccagggg cccgaggcca tggagcgccg ggtccaggct gtgcgtgaga
     8701 tccacccgtt catagatgac taccagtacg acaccgagga gagcctgtgg tgccaggtga
     8761 cagtgaagct ccctctgatg aagatcaact ttgacatgag ctccctggta gtatctttgg
     8821 cccatggtgc cgtcatctat gcgaccaagg gcatcactcg gtgcctcctg aatgaaacaa
     8881 ccaacaataa gaacgagaag gagcttgtgc taaacacaga aggaatcaac ctcccagagc
     8941 tattcaagta tgcagaggtc ctggatctgc gccgcctcta ctccaacgac atccacgcca
     9001 tagccaacac gtatggcatt gaggccgcgc tgcgggtgat cgagaaggag atcaaggatg
     9061 tgtttgccgt gtatggcatc gcggtcgacc ctcgccatct ctccctggtt gctgattata
     9121 tgtgcttcga gggtgtttac aagccactga atcgctttgg gatccggtca aactcttccc
     9181 cgctacagca gatgacattt gaaaccagct tccagtttct gaagcaagcc accatgctgg
     9241 gatcccacga tgagctgagg tctccttctg cctgccttgt ggtcgggaag gtcgtcaggg
     9301 gcgggacagg cctgttcgag ctcaagcagc ctctgagata acagctagct cgagtctaga
     9361 ggatctgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     9421 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     9481 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     9541 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     9601 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     9661 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     9721 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     9781 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     9841 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
     9901 accacgggga cgtggttttc ctttgaaaaa cacgatgata aggatccacc ggagcttacc
     9961 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
    10021 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
    10081 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
    10141 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
    10201 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
    10261 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
    10321 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
    10381 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
    10441 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
    10501 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
    10561 cgcccatcga tagatcctaa tcaacctctg gattacaaaa tttgtgaaag attgactggt
    10621 attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat
    10681 catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg
    10741 tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt
    10801 gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact
    10861 ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc
    10921 tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg
    10981 tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc
    11041 tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg
    11101 cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc
    11161 tccccgcctg agatccttta agaccaatga cttacaaggc agctgtagat cttagccact
    11221 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
    11281 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
    11341 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
    11401 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
    11461 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
    11521 agaaatgaat atcagagagt gagaggcccg ggttaattaa ggaaagggct agatcattct
    11581 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
    11641 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
    11701 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
    11761 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
    11821 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
    11881 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
    11941 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
    12001 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
    12061 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
    12121 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
    12181 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
    12241 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
    12301 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
    12361 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
    12421 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
    12481 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
    12541 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
    12601 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
    12661 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
    12721 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
    12781 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
    12841 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
    12901 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
    12961 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
    13021 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
    13081 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
    13141 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    13201 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    13261 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    13321 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    13381 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    13441 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    13501 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    13561 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
    13621 tggccgattc attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg
    13681 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    13741 taggcttttg caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt
    13801 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
    13861 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    13921 agctatgaca tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
    13981 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
    14041 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
    14101 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
    14161 tgttaaatat gtactacaaa cttagtagt
//
