LOCUS       Exported               10862 bp DNA     circular SYN 17-NOV-2021
DEFINITION  Expresses GFP-LacI-HIPK2 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10862)
  AUTHORS   Jana Haas
  TITLE     Direct Submission
  JOURNAL   Exported Nov 17, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10862
                     /organism="synthetic DNA construct"
                     /lab_host="Homo sapiens"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             937..1653
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1669..2748
                     /codon_start=1
                     /label=LacI
                     /translation="MVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSG
                     VEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFS
                     HEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWS
                     AMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSS
                     CYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASP
                     RALADSLMQLARQVSRSSLR"
     CDS             2770..2790
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2821..6396
                     /codon_start=1
                     /label=HIPK2 wt
                     /translation="MASHVQVFSPHTLQSSAFCSVKKLKIEPSSNWDMTGYGSHSKVYS
                     QSKNIPLSQPATTTVSTSLPVPNPSLPYEQTIVFPGSTGHIVVTSASSTSVTGQVLGGP
                     HNLMRRSTVSLLDTYQKCGLKRKSEEIENTSSVQIIEEHPPMIQNNASGATVATATTST
                     ATSKNSGSNSEGDYQLVQHEVLCSMTNTYEVLEFLGRGTFGQVVKCWKRGTNEIVAIKI
                     LKNHPSYARQGQIEVSILARLSTESADDYNFVRAYECFQHKNHTCLVFEMLEQNLYDFL
                     KQNKFSPLPLKYIRPVLQQVATALMKLKSLGLIHADLKPENIMLVDPSRQPYRVKVIDF
                     GSASHVSKAVCSTYLQSRYYRAPEIILGLPFCEAIDMWSLGCVIAELFLGWPLYPGASE
                     YDQIRYISQTQGLPAEYLLSAGTKTTRFFNRDTDSPYPLWRLKTPDDHEAETGIKSKEA
                     RKYIFNCLDDMAQVNMTTDLEGSDMLVEKADRREFIDLLKKMLTIDADKRITPIETLNH
                     PFVTMTHLLDFPHSTHVKSCFQNMEICKRRVNMYDTVNQSKTPFITHVAPSTSTNLTMT
                     FNNQLTTVHNQAPSSTSATISLANPEVSILNYPSTLYQPSAASMAAVAQRSMPLQTGTA
                     QICARPDPFQQALIVCPPGFQGLQASPSKHAGYSVRMENAVPIVTQAPGAQPLQIQPGL
                     LAQQAWPSGTQQILLPPAWQQLTGVATHTSVQHATVIPETMAGTQQLADWRNTHAHGSH
                     YNPIMQQPALLTGHVTLPAAQPLNVGVAHVMRQQPTSTTSSRKSKQHQSSVRNVSTCEV
                     SSSQAISSPQRSKRVKENTPPRCAMVHSSPACSTSVTCGWGDVASSTTRERQRQTIVIP
                     DTPSPTVSVITISSDTDEEEEQKHAPTSTVSKQRKNVISCVTVHDSPYSDSSSNTSPYS
                     VQQRAGHNNANAFDTKGSLENHCTGNPRTIIVPPLKTQASEVLVECDSLVPVNTSHHSS
                     SYKSKSSSNVTSTSGHSSGSSSGAITYRQQRPGPHFQQQQPLNLSQAQQHITTDRTGSH
                     RRQQAYITPTMAQAPYSFPHNSPSHGTVHPHLAAAAAAAHLPTQPHLYTYTAPAALGST
                     GTVAHLVASQGSARHTVQHTAYPASIVHQVPVSMGPRVLPSPTIHPSQYPAQFAHQTYI
                     SASPASTVYTGYPLSPAKVNQYPYI"
     CDS             3481..3483
                     /codon_start=1
                     /label=HIPK K221 wt
                     /translation="K"
     misc_feature    5452..5496
                     /label=delta SIM HIPK2
     polyA_signal    6462..6686
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6732..7160
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        7174..7503
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7354..7489
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7570..8364
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    8538..8659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8732..8748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8756..8786)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8801..8822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(9110..9695)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9866..10726)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10727..10831)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctaccgggg ccgctctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
      961 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1021 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1081 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1141 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1201 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1261 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1321 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1381 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1441 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1501 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1561 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1621 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatccat ggtgaaacca
     1681 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     1741 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     1801 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     1861 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     1921 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     1981 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     2041 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     2101 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     2161 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     2221 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     2281 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     2341 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     2401 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     2461 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     2521 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     2581 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     2641 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2701 gattcattaa tgcagctggc acgacaggtt tcccgaagca gcctgagggg gcgtcgactg
     2761 ggtacccctc ctaagaagaa gaggaaggtt gcccgggatc taggatccac tgtgtctagc
     2821 atggcctcac atgtgcaagt tttctcccct cacacccttc aatcaagtgc cttctgtagt
     2881 gtgaagaaac tgaaaataga gccgagttcc aactgggaca tgactgggta cggctcccac
     2941 agcaaagtgt atagccagag caagaacatc cccctgtcgc agccagccac cacaaccgtc
     3001 agcacctcct tgccggtccc aaacccaagc ctaccttacg agcagaccat cgtcttccca
     3061 ggaagcaccg ggcacatcgt ggtcacctca gcaagcagca cttctgtcac cgggcaagtc
     3121 ctcggcggac cacacaacct aatgcgtcga agcactgtga gcctccttga tacctaccaa
     3181 aaatgtggac tcaagcgtaa gagcgaggag atcgagaaca caagcagcgt gcagatcatc
     3241 gaggagcatc cacccatgat tcagaataat gcaagcgggg ccactgtcgc cactgccacc
     3301 acgtctactg ccacctccaa aaacagcggc tccaacagcg agggcgacta tcagctggtg
     3361 cagcatgagg tgctgtgctc catgaccaac acctacgagg tcttagagtt cttgggccga
     3421 gggacgtttg ggcaagtggt caagtgctgg aaacggggca ccaatgagat cgtagccatc
     3481 aagatcctga agaaccaccc atcctatgcc cgacaaggtc agattgaagt gagcatcctg
     3541 gcccggttga gcacggagag tgccgatgac tataacttcg tccgggccta cgaatgcttc
     3601 cagcacaaga accacacgtg cttggtcttc gagatgttgg agcagaacct ctatgacttt
     3661 ctgaagcaaa acaagtttag ccccttgccc ctcaaataca ttcgcccagt tctccagcag
     3721 gtagccacag ccctgatgaa actcaaaagc ctaggtctta tccacgctga cctcaaacca
     3781 gaaaacatca tgctggtgga tccatctaga caaccataca gagtcaaggt catcgacttt
     3841 ggttcagcca gccacgtctc caaggctgtg tgctccacct acttgcagtc cagatattac
     3901 agggcccctg agatcatcct tggtttacca ttttgtgagg caattgacat gtggtccctg
     3961 ggctgtgtta ttgcagaatt gttcctgggt tggccgttat atccaggagc ttcggagtat
     4021 gatcagattc ggtatatttc acaaacacag ggtttgcctg ctgaatattt attaagcgcc
     4081 gggacaaaga caactaggtt tttcaaccgt gacacggact caccatatcc tttgtggaga
     4141 ctgaagacac cagatgacca tgaagcagag acagggatta agtcaaaaga agcaagaaag
     4201 tacattttca actgtttaga tgatatggcc caggtgaaca tgacgacaga tttggaaggg
     4261 agcgacatgt tggtagaaaa ggctgaccgg cgggagttca ttgacctgtt gaagaagatg
     4321 ctgaccattg atgctgacaa gagaatcact ccaatcgaaa ccctgaacca tccctttgtc
     4381 accatgacac acttactcga ttttccccac agcacacacg tcaaatcatg tttccagaac
     4441 atggagatct gcaagcgtcg ggtgaatatg tatgacacgg tgaaccagag caaaacccct
     4501 ttcatcacgc acgtggcccc cagcacgtcc accaacctga ccatgacctt taacaaccag
     4561 ctgaccactg tccacaacca ggctccctcc tctaccagtg ccactatttc cttagccaat
     4621 cccgaagtct ccatactaaa ctacccatct acactctacc agccctcagc ggcatccatg
     4681 gctgcagtgg cccagcggag catgcccctg cagacaggaa cagcccagat ttgtgcccgg
     4741 cctgacccgt tccagcaagc tctcatcgtg tgtccccccg gcttccaagg cttgcaggcc
     4801 tctccctcta agcacgctgg ctactcggtg cgaatggaaa atgcagttcc catcgtcact
     4861 caagccccag gagctcagcc tcttcagatc caaccaggtc tgcttgccca gcaggcttgg
     4921 ccaagtggga cccagcagat cctgcttccc ccagcatggc agcaactgac tggagtggcc
     4981 acccacacat cagtgcagca tgccaccgtg attcccgaga ccatggcagg cacccagcag
     5041 ctggcggact ggagaaatac gcatgctcac ggaagccatt ataatcccat catgcagcag
     5101 cctgcactat tgaccggtca tgtgaccctt ccagcagcac agcccttaaa tgtgggtgtg
     5161 gcccacgtga tgcggcagca gccaaccagc accacctcct cccggaagag taagcagcac
     5221 cagtcatctg tgagaaatgt ctccacctgt gaggtgtcct cctctcaggc catcagctcc
     5281 ccacagcgat ccaagcgtgt caaggagaac acacctcccc gctgtgccat ggtgcacagt
     5341 agcccggcct gcagcacctc ggtcacctgt gggtggggcg acgtggcctc cagcaccacc
     5401 cgggaacggc agcggcagac aattgtcatt cccgacactc ccagccccac ggtcagcgtc
     5461 atcaccatca gcagtgacac ggacgaggag gaggaacaga aacacgcccc caccagcact
     5521 gtctccaagc aaagaaaaaa cgtcatcagc tgtgtcacag tccacgactc cccctactcc
     5581 gactcctcca gcaacaccag cccctactcc gtgcagcagc gtgctgggca caacaatgcc
     5641 aatgcctttg acaccaaggg gagcctggag aatcactgca cggggaaccc ccgaaccatc
     5701 atcgtgccac ccctgaaaac ccaggccagc gaagtattgg tggagtgtga tagcctggtg
     5761 ccagtcaaca ccagtcacca ctcgtcctcc tacaagtcca agtcctccag caacgtgacc
     5821 tccaccagcg gtcactcttc agggagctca tctggagcca tcacctaccg gcagcagcgg
     5881 ccgggccccc acttccagca gcagcagcca ctcaatctca gccaggctca gcagcacatc
     5941 accacggacc gcactgggag ccaccgaagg cagcaggcct acatcactcc caccatggcc
     6001 caggctccgt actccttccc gcacaacagc cccagccacg gcactgtgca cccgcatctg
     6061 gctgcagccg ctgccgctgc ccacctcccc acccagcccc acctctacac ctacactgcg
     6121 ccggcggccc tgggctccac cggcaccgtg gcccacctgg tggcctcgca aggctctgcg
     6181 cgccacaccg tgcagcacac tgcctaccca gccagcatcg tccaccaggt ccccgtgagc
     6241 atgggccccc gggtcctgcc ctcgcccacc atccacccga gtcagtatcc agcccaattt
     6301 gcccaccaga cctacatcag cgcctcgcca gcctccaccg tctacactgg atacccactg
     6361 agccccgcca aggtcaacca gtacccttac atataagaat tctcgaggat cgattaacta
     6421 gctaactaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     6481 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6541 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6601 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6661 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     6721 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     6781 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     6841 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     6901 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     6961 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7021 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7081 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7141 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     7201 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     7261 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7321 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7381 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7441 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     7501 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     7561 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     7621 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     7681 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     7741 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     7801 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     7861 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     7921 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     7981 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     8041 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     8101 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     8161 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     8221 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     8281 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     8341 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     8401 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     8461 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     8521 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8581 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8641 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     8701 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     8761 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     8821 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     8881 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     8941 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9001 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9061 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9121 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9181 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9241 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9301 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9361 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9421 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9481 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9541 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9601 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt tttgtttgca
     9661 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     9721 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     9781 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9841 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9901 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9961 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    10021 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    10081 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    10141 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    10201 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    10261 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    10321 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    10381 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    10441 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    10501 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    10561 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    10621 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    10681 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    10741 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    10801 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10861 tc
//
