LOCUS       Exported               14469 bp DNA     circular SYN 17-NOV-2021
DEFINITION  Expresses GFP-LacI-p300 fusion protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Maximilian Pfisterer
  TITLE     Direct Submission
  JOURNAL   Exported Nov 17, 2021 from SnapGene 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="recombinant plasmid"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             937..1653
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1669..2748
                     /codon_start=1
                     /label=LacI
                     /translation="MVKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSG
                     VEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFS
                     HEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWS
                     AMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSS
                     CYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASP
                     RALADSLMQLARQVSRSSLR"
     primer_bind     2608..2627
                     /label=pSV_GFP_LacI_seq
     CDS             2767..10008
                     /codon_start=1
                     /label=p300
                     /translation="MAENVVEPGPPSAKRPKLSSPALSASASDGTDFGSLFDLEHDLPD
                     ELINSTELGLTNGGDINQLQTSLGMVQDAASKHKQLSELLRSGSSPNLNMGVGGPGQVM
                     ASQAQQSSPGLGLINSMVKSPMTQAGLTSPNMGMGTSGPNQGPTQSTGMMNSPVNQPAM
                     GMNTGMNAGMNPGMLAAGNGQGIMPNQVMNGSIGAGRGRQNMQYPNPGMGSAGNLLTEP
                     LQQGSPQMGGQTGLRGPQPLKMGMMNNPNPYGSPYTQNPGQQIGASGLGLQIQTKTVLS
                     NNLSPFAMDKKAVPGGGMPNMGQQPAPQVQQPGLVTPVAQGMGSGAHTADPEKRKLIQQ
                     QLVLLLHAHKCQRREQANGEVRQCNLPHCRTMKNVLNHMTHCQSGKSCQVAHCASSRQI
                     ISHWKNCTRHDCPVCLPLKNAGDKRNQQPILTGAPVGLGNPSSLGVGQQSAPNLSTVSQ
                     IDPSSIERAYAALGLPYQVNQMPTQPQVQAKNQQNQQPGQSPQGMRPMSNMSASPMGVN
                     GGVGVQTPSLLSDSMLHSAINSQNPMMSENASVPSLGPMPTAAQPSTTGIRKQWHEDIT
                     QDLRNHLVHKLVQAIFPTPDPAALKDRRMENLVAYARKVEGDMYESANNRAEYYHLLAE
                     KIYKIQKELEEKRRTRLQKQNMLPNAAGMVPVSMNPGPNMGQPQPGMTSNGPLPDPSMI
                     RGSVPNQMMPRITPQSGLNQFGQMSMAQPPIVPRQTPPLQHHGQLAQPGALNPPMGYGP
                     RMQQPSNQGQFLPQTQFPSQGMNVTNIPLAPSSGQAPVSQAQMSSSSCPVNSPIMPPGS
                     QGSHIHCPQLPQPALHQNSPSPVPSRTPTPHHTPPSIGAQQPPATTIPAPVPTPPAMPP
                     GPQSQALHPPPRQTPTPPTTQLPQQVQPSLPAAPSADQPQQQPRSQQSTAASVPTPTAP
                     LLPPQPATPLSQPAVSIEGQVSNPPSTSSTEVNSQAIAEKQPSQEVKMEAKMEVDQPEP
                     ADTQPEDISESKVEDCKMESTETEERSTELKTEIKEEEDQPSTSATQSSPAPGQSKKKI
                     FKPEELRQALMPTLEALYRQDPESLPFRQPVDPQLLGIPDYFDIVKSPMDLSTIKRKLD
                     TGQYQEPWQYVDDIWLMFNNAWLYNRKTSRVYKYCSKLSEVFEQEIDPVMQSLGYCCGR
                     KLEFSPQTLCCYGKQLCTIPRDATYYSYQNRYHFCEKCFNEIQGESVSLGDDPSQPQTT
                     INKEQFSKRKNDTLDPELFVECTECGRKMHQICVLHHEIIWPAGFVCDGCLKKSARTRK
                     ENKFSAKRLPSTRLGTFLENRVNDFLRRQNHPESGEVTVRVVHASDKTVEVKPGMKARF
                     VDSGEMAESFPYRTKALFAFEEIDGVDLCFFGMHVQEYGSDCPPPNQRRVYISYLDSVH
                     FFRPKCLRTAVYHEILIGYLEYVKKLGYTTGHIWACPPSEGDDYIFHCHPPDQKIPKPK
                     RLQEWYKKMLDKAVSERIVHDYKDIFKQATEDRLTSAKELPYFEGDFWPNVLEESIKEL
                     EQEEEERKREENTSNESTDVTKGDSKNAKKKNNKKTSKNKSSLSRGNKKKPGMPNVSND
                     LSQKLYATMEKHKEVFFVIRLIAGPAANSLPPIVDPDPLIPCDLMDGRDAFLTLARDKH
                     LEFSSLRRAQWSTMCMLVELHTQSQDRFVYTCNECKHHVETRWHCTVCEDYDLCITCYN
                     TKNHDHKMEKLGLGLDDESNNQQAAATQSPGDSRRLSIQRCIQSLVHACQCRNANCSLP
                     SCQKMKRVVQHTKGCKRKTNGGCPICKQLIALCCYHAKHCQENKCPVPFCLNIKQKLRQ
                     QQLQHRLQQAQMLRRRMASMQRTGVVGQQQGLPSPTPATPTTPTGQQPTTPQTPQPTSQ
                     PQPTPPNSMPPYLPRTQAAGPVSQGKAAGQVTPPTPPQTAQPPLPGPPPAAVEMAMQIQ
                     RAAETQRQMAHVQIFQRPIQHQMPPMTPMAPMGMNPPPMTRGPSGHLEPGMGPTGMQQQ
                     PPWSQGGLPQPQQLQSGMPRPAMMSVAQHGQPLNMAPQPGLGQVGISPLKPGTVSQQAL
                     QNLLRTLRSPSSPLQQQQVLSIIHANPQLLAAFIKQRAAKYANSNPQPIPGQPGMPQGQ
                     PGLQPPTMPGQQGVHSNPAMQNMNPMQAGVQRAGLPQQQPQQQLQPPMGGMSPQAQQMN
                     MNHNTMPSQFRDILRRQQMMQQQQQQGAGPGIGPGMANHNQFQQPQGVGYPPQQQQRMQ
                     HHMQQMQQGNMGQIGQLPQALGAEAGASLQAYQQRLLQQQMGSPVQPNPMSPQQHMLPN
                     QAQSPHLQGQQIPNSLSNQVRSPQPVPSPRPQSQPPHSSPSPRMQPQPSPHHVSPQTSS
                     PHPGLVAAQANPMEQGHFASPDQNSMLSQLASNPGMANLHGASATDLGLSTDNSDLNSN
                     LSQSTLDIH"
     CDS             5908..7758
                     /gene="EP300"
                     /product="catalytic histone acetyltransferase core of the 
                     human E1A-associated protein p300 (Delvecchio et al., 
                     2013)"
                     /label=p300 core
     primer_bind     complement(10063..10080)
                     /label=BGH-rev
     polyA_signal    10069..10293
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10339..10767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        10781..11110
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10961..11096
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11177..11971
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    12145..12266
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12315..12331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12339..12355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12363..12393)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12408..12429
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(12717..13302)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13473..14333)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14334..14438)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gctaccgggg ccgctctagc gctaccggtc gccaccatgg tgagcaaggg cgaggagctg
      961 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1021 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1081 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1141 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1201 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1261 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1321 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1381 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1441 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1501 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     1561 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1621 gggatcactc tcggcatgga cgagctgtac aagtccggac tcagatccat ggtgaaacca
     1681 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg
     1741 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg
     1801 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg
     1861 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt
     1921 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc
     1981 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc
     2041 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt
     2101 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat
     2161 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg
     2221 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa
     2281 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg
     2341 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg
     2401 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc
     2461 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc
     2521 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc
     2581 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg
     2641 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2701 gattcattaa tgcagctggc acgacaggtt tcccgaagca gcctgagggg gcgtcgactg
     2761 ggtaccatgg ccgagaatgt ggtggaaccg gggccgcctt cagccaagcg gcctaaactc
     2821 tcatctccgg ccctctcggc gtccgccagc gatggcacag attttggctc tctatttgac
     2881 ttggagcacg acttaccaga tgaattaatc aactctacag aattgggact aaccaatggt
     2941 ggtgatatta atcagcttca gacaagtctt ggcatggtac aagatgcagc ttctaaacat
     3001 aaacagctgt cagaattgct gcgatctggt agttccccta acctcaatat gggagttggt
     3061 ggcccaggtc aagtcatggc cagccaggcc caacagagca gtcctggatt aggtttgata
     3121 aatagcatgg tcaaaagccc aatgacacag gcaggcttga cttctcccaa catggggatg
     3181 ggcactagtg gaccaaatca gggtcctacg cagtcaacag gtatgatgaa cagtccagta
     3241 aatcagcctg ccatgggaat gaacacaggg atgaatgcgg gcatgaatcc tggaatgttg
     3301 gctgcaggca atggacaagg gataatgcct aatcaagtca tgaacggttc aattggagca
     3361 ggccgagggc gacagaatat gcagtaccca aacccaggca tgggaagtgc tggcaactta
     3421 ctgactgagc ctcttcagca gggctctccc cagatgggag gacaaacagg attgagaggc
     3481 ccccagcctc ttaagatggg aatgatgaac aaccccaatc cttatggttc accatatact
     3541 cagaatcctg gacagcagat tggagccagt ggccttggtc tccagattca gacaaaaact
     3601 gtactatcaa ataacttatc tccatttgct atggacaaaa aggcagttcc tggtggagga
     3661 atgcccaaca tgggtcaaca gccagccccg caggtccagc agccaggtct ggtgactcca
     3721 gttgcccaag ggatgggttc tggagcacat acagctgatc cagagaagcg caagctcatc
     3781 cagcagcagc ttgttctcct tttgcatgct cacaagtgcc agcgccggga acaggccaat
     3841 ggggaagtga ggcagtgcaa ccttccccac tgtcgcacaa tgaagaatgt cctaaaccac
     3901 atgacacact gccagtcagg caagtcttgc caagtggcac actgtgcatc ttctcgacaa
     3961 atcatttcac actggaagaa ttgtacaaga catgattgtc ctgtgtgtct ccccctcaaa
     4021 aatgctggtg ataagagaaa tcaacagcca attttgactg gagcacccgt tggacttgga
     4081 aatcctagct ctctaggggt gggtcaacag tctgccccca acctaagcac tgttagtcag
     4141 attgatccca gctccataga aagagcctat gcagctcttg gactacccta tcaagtaaat
     4201 cagatgccga cacaacccca ggtgcaagca aagaaccagc agaatcagca gcctgggcag
     4261 tctccccaag gcatgcggcc catgagcaac atgagtgcta gtcctatggg agtaaatgga
     4321 ggtgtaggag ttcaaacgcc gagtcttctt tctgactcaa tgttgcattc agccataaat
     4381 tctcaaaacc caatgatgag tgaaaatgcc agtgtgccct ccctgggtcc tatgccaaca
     4441 gcagctcaac catccactac tggaattcgg aaacagtggc acgaagatat tactcaggat
     4501 cttcgaaatc atcttgttca caaactcgtc caagccatat ttcctacgcc ggatcctgct
     4561 gctttaaaag acagacggat ggaaaaccta gttgcatatg ctcggaaagt tgaaggggac
     4621 atgtatgaat ctgcaaacaa tcgagcggaa tactaccacc ttctagctga gaaaatctat
     4681 aagatccaga aagaactaga agaaaaacga aggaccagac tacagaagca gaacatgcta
     4741 ccaaatgctg caggcatggt tccagtttcc atgaatccag ggcctaacat gggacagccg
     4801 caaccaggaa tgacttctaa tggccctcta cctgacccaa gtatgatccg tggcagtgtg
     4861 ccaaaccaga tgatgcctcg aataactcca caatctggtt tgaatcaatt tggccagatg
     4921 agcatggccc agccccctat tgtaccccgg caaacccctc ctcttcagca ccatggacag
     4981 ttggctcaac ctggagctct caacccgcct atgggctatg ggcctcgtat gcaacagcct
     5041 tccaaccagg gccagttcct tcctcagact cagttcccat cacagggaat gaatgtaaca
     5101 aatatccctt tggctccgtc cagcggtcaa gctccagtgt ctcaagcaca aatgtctagt
     5161 tcttcctgcc cggtgaactc tcctataatg cctccagggt ctcaggggag ccacattcac
     5221 tgtccccagc ttcctcaacc agctcttcat cagaattcac cctcgcctgt acctagtcgt
     5281 acccccaccc ctcaccatac tcccccaagc ataggggctc agcagccacc agcaacaaca
     5341 attccagccc ctgttcctac acctcctgcc atgccacctg ggccacagtc ccaggctcta
     5401 catccccctc caaggcagac acctacacca ccaacaacac aacttcccca acaagtgcag
     5461 ccttcacttc ctgctgcacc ttctgctgac cagccccagc agcagcctcg ctcacagcag
     5521 agcacagcag cgtctgttcc taccccaaca gcaccgctgc ttcctccgca gcctgcaact
     5581 ccactttccc agccagctgt aagcattgaa ggacaggtat caaatcctcc atctactagt
     5641 agcacagaag tgaattctca ggccattgct gagaagcagc cttcccagga agtgaagatg
     5701 gaggccaaaa tggaagtgga tcaaccagaa ccagcagata ctcagccgga ggatatttca
     5761 gagtctaaag tggaagactg taaaatggaa tctaccgaaa cagaagagag aagcactgag
     5821 ttaaaaactg aaataaaaga ggaggaagac cagccaagta cttcagctac ccagtcatct
     5881 ccggctccag gacagtcaaa gaaaaagatt ttcaaaccag aagaactacg acaggcactg
     5941 atgccaactt tggaggcact ttaccgtcag gatccagaat cccttccctt tcgtcaacct
     6001 gtggaccctc agcttttagg aatccctgat tactttgata ttgtgaagag ccccatggat
     6061 ctttctacca ttaagaggaa gttagacact ggacagtatc aggagccctg gcagtatgtc
     6121 gatgatattt ggcttatgtt caataatgcc tggttatata accggaaaac atcacgggta
     6181 tacaaatact gctccaagct ctctgaggtc tttgaacaag aaattgaccc agtgatgcaa
     6241 agccttggat actgttgtgg cagaaagttg gagttctctc cacagacact gtgttgctac
     6301 ggcaaacagt tgtgcacaat acctcgtgat gccacttatt acagttacca gaacaggtat
     6361 catttctgtg agaagtgttt caatgagatc caaggggaga gcgtttcttt gggggatgac
     6421 ccttcccagc ctcaaactac aataaataaa gaacaatttt ccaagagaaa aaatgacaca
     6481 ctggatcctg aactgtttgt tgaatgtaca gagtgcggaa gaaagatgca tcagatctgt
     6541 gtccttcacc atgagatcat ctggcctgct ggattcgtct gtgatggctg tttaaagaaa
     6601 agtgcacgaa ctaggaaaga aaataagttt tctgctaaaa ggttgccatc taccagactt
     6661 ggcacctttc tagagaatcg tgtgaatgac tttctgaggc gacagaatca ccctgagtca
     6721 ggagaggtca ctgttagagt agttcatgct tctgacaaaa ccgtggaagt aaaaccaggc
     6781 atgaaagcaa ggtttgtgga cagtggagag atggcagaat cctttccata ccgaaccaaa
     6841 gccctctttg cctttgaaga aattgatggt gttgacctgt gcttctttgg catgcatgtt
     6901 caagagtatg gctctgactg ccctccaccc aaccagagga gagtatacat atcttacctc
     6961 gatagtgttc atttcttccg tcctaaatgc ttgaggactg cagtctatca tgaaatccta
     7021 attggatatt tagaatatgt caagaaatta ggttacacaa cagggcatat ttgggcatgt
     7081 ccaccaagtg agggagatga ttatatcttc cattgccatc ctcctgacca gaagataccc
     7141 aagcccaagc gactgcagga atggtacaaa aaaatgcttg acaaggctgt atcagagcgt
     7201 attgtccatg actacaagga tatttttaaa caagctactg aagatagatt aacaagtgca
     7261 aaggaattgc cttatttcga gggtgatttc tggcccaatg ttctggaaga aagcattaag
     7321 gaactggaac aggaggaaga agagagaaaa cgagaggaaa acaccagcaa tgaaagcaca
     7381 gatgtgacca agggagacag caaaaatgct aaaaagaaga ataataagaa aaccagcaaa
     7441 aataagagca gcctgagtag gggcaacaag aagaaacccg ggatgcccaa tgtatctaac
     7501 gacctctcac agaaactata tgccaccatg gagaagcata aagaggtctt ctttgtgatc
     7561 cgcctcattg ctggccctgc tgccaactcc ctgcctccca ttgttgatcc tgatcctctc
     7621 atcccctgcg atctgatgga tggtcgggat gcgtttctca cgctggcaag ggacaagcac
     7681 ctggagttct cttcactccg aagagcccag tggtccacca tgtgcatgct ggtggagctg
     7741 cacacgcaga gccaggaccg ctttgtctac acctgcaatg aatgcaagca ccatgtggag
     7801 acacgctggc actgtactgt ctgtgaggat tatgacttgt gtatcacctg ctataacact
     7861 aaaaaccatg accacaaaat ggagaaacta ggccttggct tagatgatga gagcaacaac
     7921 cagcaggctg cagccaccca gagcccaggc gattctcgcc gcctgagtat ccagcgctgc
     7981 atccagtctc tggtccatgc ttgccagtgt cggaatgcca attgctcact gccatcctgc
     8041 cagaagatga agcgggttgt gcagcatacc aagggttgca aacggaaaac caatggcggg
     8101 tgccccatct gcaagcagct cattgccctc tgctgctacc atgccaagca ctgccaggag
     8161 aacaaatgcc cggtgccgtt ctgcctaaac atcaagcaga agctccggca gcaacagctg
     8221 cagcaccgac tacagcaggc ccaaatgctt cgcaggagga tggccagcat gcagcggact
     8281 ggtgtggttg ggcagcaaca gggcctccct tcccccactc ctgccactcc aacgacacca
     8341 actggccaac agccaaccac cccgcagacg ccccagccca cttctcagcc tcagcctacc
     8401 cctcccaata gcatgccacc ctacttgccc aggactcaag ctgctggccc tgtgtcccag
     8461 ggtaaggcag caggccaggt gacccctcca acccctcctc agactgctca gccacccctt
     8521 ccagggcccc cacctgcagc agtggaaatg gcaatgcaga ttcagagagc agcggagacg
     8581 cagcgccaga tggcccacgt gcaaattttt caaaggccaa tccaacacca gatgcccccg
     8641 atgactccca tggcccccat gggtatgaac ccacctccca tgaccagagg tcccagtggg
     8701 catttggagc cagggatggg accgacaggg atgcagcaac agccaccctg gagccaagga
     8761 ggattgcctc agccccagca actacagtct gggatgccaa ggccagccat gatgtcagtg
     8821 gcccagcatg gtcaaccttt gaacatggct ccacaaccag gattgggcca ggtaggtatc
     8881 agcccactca aaccaggcac tgtgtctcaa caagccttac aaaacctttt gcggactctc
     8941 aggtctccca gctctcccct gcagcagcaa caggtgctta gtatcattca cgccaacccc
     9001 cagctgttgg ctgcattcat caagcagcgg gctgccaagt atgccaactc taatccacaa
     9061 cccatccctg ggcagcctgg catgccccag gggcagccag ggctacagcc acctaccatg
     9121 ccaggtcagc agggggtcca ctccaatcca gccatgcaga acatgaatcc aatgcaggcg
     9181 ggcgttcaga gggctggcct gccccagcag caaccacagc agcaactcca gccacccatg
     9241 ggagggatga gcccccaggc tcagcagatg aacatgaacc acaacaccat gccttcacaa
     9301 ttccgagaca tcttgagacg acagcaaatg atgcaacagc agcagcaaca gggagcaggg
     9361 ccaggaatag gccctggaat ggccaaccat aaccagttcc agcaacccca aggagttggc
     9421 tacccaccac agcagcagca gcggatgcag catcacatgc aacagatgca acaaggaaat
     9481 atgggacaga taggccagct tccccaggcc ttgggagcag aggcaggtgc cagtctacag
     9541 gcctatcagc agcgactcct tcagcaacag atggggtccc ctgttcagcc caaccccatg
     9601 agcccccagc agcatatgct cccaaatcag gcccagtccc cacacctaca aggccagcag
     9661 atccctaatt ctctctccaa tcaagtgcgc tctccccagc ctgtcccttc tccacggcca
     9721 cagtcccagc ccccccactc cagtccttcc ccaaggatgc agcctcagcc ttctccacac
     9781 cacgtttccc cacagacaag ttccccacat cctggactgg tagctgccca ggccaacccc
     9841 atggaacaag ggcattttgc cagcccggac cagaattcaa tgctttctca gcttgctagc
     9901 aatccaggca tggcaaacct ccatggtgca agcgccacgg acctgggact cagcaccgat
     9961 aactcagact tgaattcaaa cctctcacag agtacactag acatacacct cgaggatcga
    10021 ttaagtagct aactagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
    10081 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
    10141 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
    10201 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
    10261 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
    10321 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
    10381 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
    10441 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
    10501 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
    10561 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
    10621 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    10681 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    10741 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
    10801 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
    10861 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
    10921 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
    10981 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
    11041 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
    11101 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
    11161 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    11221 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    11281 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    11341 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    11401 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    11461 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    11521 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    11581 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    11641 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
    11701 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    11761 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    11821 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    11881 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    11941 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
    12001 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
    12061 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    12121 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    12181 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    12241 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    12301 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    12361 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    12421 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    12481 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    12541 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    12601 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    12661 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    12721 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    12781 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    12841 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    12901 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    12961 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    13021 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    13081 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    13141 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    13201 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggttttttt
    13261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    13321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    13381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    13441 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    13501 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    13561 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    13621 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    13681 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    13741 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    13801 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    13861 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    13921 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    13981 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    14041 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    14101 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    14161 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    14221 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    14281 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    14341 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    14401 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    14461 cctgacgtc
//
