LOCUS       Exported               10400 bp DNA     circular SYN 17-NOV-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pPOLY_new
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10400)
  AUTHORS   alenavoit
  TITLE     Direct Submission
  JOURNAL   Exported Nov 17, 2021 from SnapGene Viewer 5.3.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10400
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     10..42
                     /label=pBP2594 PT7 fw
     promoter        10..28
                     /label=T7 promoter
                     /note="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    29..53
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator 
                     toinhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     53..67
                     /label=pBP2565
     misc_feature    68
                     /label=G
     RBS             84..89
                     /label=T7 RBS
                     /note="ribosome binding site"
     CDS             97..99
                     /codon_start=1
                     /label=ATG
                     /translation="M"
     primer_bind     109..128
                     /label=pBP2614 Bsp1407I-strong RBS-BcuI-CcCDP_CO fw
     CDS             109..126
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHIS
                     /note="6xHis"
                     /translation="HHHHHH"
     misc_feature    136..2601
                     /product="Cellobiose phosphorylase from Cellulomonas 
                     uda_CO"
                     /label=cellobiose phosphorylase
                     /note="codon optimized sequence for E. coli (IDT)
                     additionally optimized for less restriction sites
                     "
     primer_bind     2598..2657
                     /label=pBP2614 Bsp1407I-strong RBS-BcuI-CcCDP_CO fw
     RBS             2608..2619
                     /label=strong RBS
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     2619..2671
                     /label=pBP2565
     primer_bind     2625..2686
                     /label=pET21-NdeI-6xHis-CcCDPase fw
     primer_bind     2625..2661
                     /label=pBP2558 NdeI-CcCDP_CO fw
     CDS             2626..2628
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation="M"
     CDS             2638..2655
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHIS
                     /translation="HHHHHH"
     primer_bind     2647..2676
                     /label=BamHI-CcCDP_CO fw
     CDS             2662..5616
                     /codon_start=1
                     /product="Cellodextrin Phosphorylase from Clostridium 
                     cellulosi"
                     /label=cellodextrin phosphorylase
                     /translation="ITKVTPRSCKLTPFEILKQKFGSIIAMSGCADAIYSDSSCKNVIG
                     ITGLPIKAPVIQVLLENSKLAAFLNEYVQNKSVCLALEGMRYCLVFEDGQKVKLPQELD
                     DIIQSTVSDVKNWAGTLTENGEHVIDLKKPSPGPHFYVNLLIGNRLGFNRTLQTTPKSV
                     VDRFGRGSFRSHAATQVLATRYDMRQEENGFPANRQFYLYENGKQIFYSGSVDDDNILE
                     AKCIHSCNRTVIKYKTACKLEITRTIFLCPQKKGLPLATEIQIINIKNNSERKRNLSIT
                     YTGMFGTAATHAIFEDVTYTNVIMQSAALYNDNGEFIGITPDYYRKKYNEDTRFVTMFV
                     RKDGKRIFPKSFCTDYNDFVGNGTLEHPAGGEHLNNKLNRKGPGFFALGTPFTIMPGQD
                     IEIDTFTGMSSSKDNPNYDDTTMPKELEALLRYFEASDSVQSALREVIDFQEKYCKYIE
                     FKTEDKMFNAGFNKNLAFQILYQTFMSRSFCQTQKGYREIGFREIQDLYASMYYFINLG
                     YQDFVKNLLFEWTSNVYKMGYANHNFYWVGKQPGLYSDDSLWLLQAYYRYIIYTKDTSI
                     LNEEVPVADGNNEKRAVRDTLKAIIQYSAFISVGNHGLPLLDLADWNDCLKIDNDYVDG
                     AQKEKLYYKQLKETGGKYGDRFMSDYSESVMNAFLLKVAVDDLAEIAELDKDAELEQSM
                     RKISSALSEKIQKYAWKGNFFARVLLNRYKDGSFTYVGAKGDKLSADPNIDGTYFLNSF
                     SWAILSGVATDEQIGIMVDTLKKYMLTPYGIRLTTPADLNKLANDTATDHYFYGDRENG
                     AVFKHASMMATAALAKAAKSVKDKALAKEMARLAYYMIDLVLPYKNLEDPFKLAGNPRI
                     CTQYINADSGENIGPLLSGTATWLNLTITSLAGIEYVKDGIMFSPVLREDETRLEYSLK
                     APGCSYNITITKPRGFARLIDSAYDLRIDGKKTDNCIIPMFTDGKEHSVTLEFK"
     primer_bind     2662..2686
                     /label=Primer 1
     primer_bind     complement(2666..2688)
                     /label=pBP2600 CcCDP rev
     primer_bind     3311..3330
                     /label=CcCDP_CO sequ fw
     primer_bind     complement(3492..3513)
                     /label=pBP2616
     primer_bind     4753..4768
                     /label=pBP2617
     primer_bind     complement(4962..4981)
                     /label=CcCDP_CO sequ rev
     primer_bind     5197..5226
                     /label=pBP2601 CcCDP fw
     primer_bind     5477..5497
                     /label=pET21-NdeI-6xHis-CcCDPase fw
     primer_bind     complement(5581..5616)
                     /label=pBP2595 T7TT rev
     primer_bind     complement(5585..5617)
                     /label=pET21-XhoI-CcCDPase rev
     primer_bind     complement(5587..5650)
                     /label=pBP2566
     primer_bind     complement(5587..5633)
                     /label=pBP2615 Bsp1407I-MunI-CcCDP_CO rev
     primer_bind     complement(5587..5616)
                     /label=HindIII-CcCDP_CO rev
     primer_bind     complement(5589..5644)
                     /label=pBP2559 NdeI-RBS-NotI-CcCDP_CO rev
     RBS             5629..5640
                     /label=strong RBS
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             5647..5649
                     /codon_start=1
                     /product="start codon"
                     /label=ATG
                     /translation="M"
     misc_feature    5651..5670
                     /label=OE
     CDS             5656..5679
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5686..7200
                     /codon_start=1
                     /label=sucrose phosphorylase
                     /translation="MKNKVQLITYADRLGDGTIKSMTDILRTRFDGVYDGVHILPFFTP
                     FDGADAGFDPIDHTKVDERLGSWDDVAELSKTHNIMVDAIVNHMSWESKQFQDVLAKGE
                     ESEYYPMFLTMSSVFPNGATEEDLAGIYRPRPGLPFTHYKFAGKTRLVWVSFTPQQVDI
                     DTDSDKGWEYLMSIFDQMAASHVSYIRLDAVGYGAKEAGTSCFMTPKTFKLISRLREEG
                     VKRGLEILIEVHSYYKKQVEIASKVDRVYDFALPPLLLHALSTGHVEPVAHWTDIRPNN
                     AVTVLDTHDGIGVIDIGSDQLDRSLKGLVPDEDVDNLVNTIHANTHGESQAATGAAASN
                     LDLYQVNSTYYSALGCNDQHYIAARAVQFFLPGVPQVYYVGALAGKNDMELLRKTNNGR
                     DINRHYYSTAEIDENLKRPVVKALNALAKFRNELDAFDGTFSYTTDDDTSISFTWRGET
                     SQATLTFEPKRGLGVDNTTPVAMLEWEDSAGDHRSDDLIANPPVVA"
     primer_bind     7207..7256
                     /label=pBP2595 T7TT rev
     terminator      7207..7254
                     /label=T7 terminator
     misc_feature    complement(7207..7252)
                     /label=T7 terminator [3']
     terminator      7261..7355
                     /label=lambda t0
                     /label=lamda t0 terminator
     misc_feature    7356
                     /label=PART 1
     misc_feature    7379..7519
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7705..8293)
                     /direction=LEFT
                     /label=pBR322 ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    8311..8351
                     /label=PART 1
     terminator      8316..8345
                     /label=rrnC terminator
                     /label=rrnC
                     /note="E. colistrain K-12 substrain MG1655 (NCBI sequence 
                     ID: gb|CP012870.1)"
     misc_feature    8346..9239
                     /label=PART 2
     CDS             complement(8352..9212)
                     /codon_start=1
                     /label=AmpR
                     /note="AmpR (from pQE80L)2 mutations compared to original 
                     sequence encoded on pBR322
                     "
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(8352..8354)
                     /label=Stop AmpR
     misc_feature    complement(9210..9212)
                     /label=Start AmpR
     RBS             9219..9230
                     /label=strong RBS
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_feature    join(9234..10400,1..6)
                     /label=PART 3
     CDS             complement(9240..10322)
                     /codon_start=1
                     /label=LacI
                     /label=LacI (Ecogene EG10525)
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     promoter        complement(10323..10400)
                     /label=PlacIq
                     /note="mutant lacIQ promoter; contains an ""up"" mutation 
                     in the -35 box at position -35 of the lacI promoter (Calos 
                     (1978) Nature 274(5673): 762-5)"
     -10_signal      10357..10363
                     /label=-10 box
                     /note="Pribnow box (-10 signal) of the lacI promoter"
     misc_feature    10384..10388
                     /label=-35 box
     misc_feature    10385
                     /label=lacIq mutation
                     /note="""up"" mutation in the -35 box at position -35 of 
                     the lacI promoter, lacIq promoter (Calos (1978) Nature 
                     274(5673): 762-5)"
ORIGIN
        1 gaattcagtt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
       61 aaataatgtt gtttaacttt aagaaggaga tatcatatgg gcagcagcca tcaccatcac
      121 catcacgggt cgacccgcta tggtcatttt gacgatgaag cacgcgagta cgttatcacc
      181 acaccgcata ctccgtaccc ttggatcaac tatctgggtt cggaacagtt cttcagtctt
      241 ttgtcgcacc aggctggcgg ctattcgttt tatcgcgatg ccaagatgcg tagattgaca
      301 agatacagat acaacaacat accagcagac gcgggaggcc ggtatcttta tgtaaacgac
      361 ggtggggacg tttggacgcc atcgtggctt cctgttaaag cagacttaga tcattttgaa
      421 gccagacatg gtcttggcta cagtaccatt acaggtgaaa gaaatggggt acgggttgag
      481 accttgtttt tcgtcccagt cggtgagaac gcggaggtcc agaaagttac ggtcactaat
      541 acctctgact cgtataaatc cctgacgttg ttttcttttg tcgaattttg tctttggaac
      601 gcgcaagacg atcaaactaa ctaccagcgt aatctgagca taggagaggt tgaagtggaa
      661 caagaatctc cgcatggcag tgcaatttat caccgtacag agtaccggga gagacgcgat
      721 cactacgcgg tattcgccgt taatacgcag gccgagggtt tcgatacaga tcgcgacaca
      781 tttgtgggag cctataactc gcttggtgaa gccgcagttc cccttaaggg agaaagtgca
      841 aatagcgttg caagtggatg gtatcctatt ggctctcata gcgtggcagt gtctttagcg
      901 cctggagaat ccagagaatt ggtttatgtg cttgggtacg tggaaaatcc tgatgaggag
      961 aagtgggcag acgacgcaaa acaggtcgtg aataaagaaa gagctcacgc tttattatca
     1021 agatttgcta cgtccgaaca gactgacgca gctttcgccg ctttgaagga ttattggacg
     1081 gatttgttat cgacgtattc cgttagttcc aatgacgaaa aattggaccg gatggttaat
     1141 atatggaatc agtaccaatg catggtaacc tttaacatga gtcgctcggc gagctttttc
     1201 gagaccggta ttgggagagg gatggggttt cgtgatagca accaagacct tctgggcttt
     1261 gttcacttaa tcccagagcg ggcgcgtgag cgcatcattg acatagcgtc aacccagttc
     1321 gcggacggtt ccgcttatca ccaataccaa cctttaacga agcgcggtaa caacgacatt
     1381 ggctctgggt ttaacgacga tcctctttgg ttaatcgcgg gaactgccgc ctacatcaaa
     1441 gaaactggtg acttctccat attagatgag cccgtacctt ttgataacga accggggtca
     1501 gaagttccgt tatttgagca cctgacccgt agttttgagt ttaccgtaac ccatcggggt
     1561 ccccacggtt tgcctcttat tggccgcgca gattggaacg attgtttgaa tcttaattgc
     1621 ttcagtacaa ccccgggcga gtcatttcag acaacggaga accaagccgg tggcgtagcg
     1681 gagtcaacgt tcatagccgc gcaattcgtt ttatatgggg agcagtatgc cgagttggca
     1741 gcgcggcgcg ggttggccga cgtagccgat agagcaagag ggcatgtggc tgaaatgaga
     1801 gacgcacttc tgaccgacgg gtgggacggc agctggttct tgagagctta tgactattat
     1861 ggaaacccta tcggcaccga cgcacatgac gaagggaaga tttggatcga accgcaaggg
     1921 ttcgcggtga tggctggagt cggtgtaggc gaagggcctc aagatacgga cgctcccgct
     1981 attaaggcgc ttgattctgt gaacgaaatg ttggcaaccg atcacggtat ggtccttcaa
     2041 tacccagcat acactacata ccaagtacac atgggtgaag tatccactta tccacccggg
     2101 tataaagaga atggaggtat attttgccac aataatcctt gggttatcat agcggaaact
     2161 gtcgtcggaa gaggcggacg ggcatttgat tactacaaga gaattactcc cgcgtatcgc
     2221 gaggacataa gcgacgttca ccgtcttgaa ccctatgtct atgcacaaat gatagcaggg
     2281 aaggaggcgg tccgtcacgg tgaagcaaag aacagctggc ttaccgggac tgcggcttgg
     2341 aactttgtga ccgtgtcaca gtaccttctt ggggttcgcc cagagtacga cggcttggtg
     2401 gtagacccgc agatcggacc tgacgtacct agctttacgg ttacgcgtgt cgcccgtgga
     2461 gccacttacg agataacagt cactaacagt gggaccgacg gatcacgcgg tcgccttgtc
     2521 gttgatggaa cacctgtaga aggcaacttg gttccttatg cccccgcagg gtcaacagtt
     2581 cgtgtcgatg tgacattgta gtgtacaaaa gaggagaaaa ctagtatgag aggatcgcat
     2641 caccatcacc atcacggatc cattaccaaa gtgaccccgc gtagctgcaa actgaccccg
     2701 ttcgagattc tgaaacaaaa attcggtagc attattgcga tgagcggctg cgcggatgcg
     2761 atctacagcg acagcagctg caagaacgtg atcggtatta ccggcctgcc gatcaaagcg
     2821 ccggtgattc aggttctgct ggagaacagc aagctggcgg cgttcctgaa cgaatacgtg
     2881 caaaacaaaa gcgtttgcct ggcgctggag ggtatgcgtt attgcctggt gtttgaagac
     2941 ggccagaagg ttaaactgcc gcaagagctg gacgatatca ttcagagcac cgtgagcgat
     3001 gttaaaaact gggcgggtac cctgaccgag aacggcgaac acgtgatcga cctgaagaaa
     3061 ccgagcccgg gtccgcactt ctacgttaac ctgctgattg gtaaccgtct gggctttaac
     3121 cgtaccctgc aaaccacccc gaagagcgtg gttgatcgtt tcggtcgtgg cagctttcgt
     3181 agccatgcgg cgacccaggt tctggcgacc cgttacgaca tgcgtcaaga ggaaaacggc
     3241 ttcccggcga accgtcagtt ttacctgtat gagaacggca agcaaatctt ctacagcggc
     3301 agcgtggacg atgacaacat cctggaagcg aaatgcattc acagctgcaa ccgtaccgtt
     3361 attaagtata aaaccgcgtg caagctggag atcacccgta ccatttttct gtgcccgcag
     3421 aagaaaggtc tgccgctggc gaccgagatc caaatcatca acatcaagaa caacagcgaa
     3481 cgtaaacgta acctgagcat cacctacacc ggcatgtttg gtaccgcggc gacccacgcg
     3541 atctttgaag atgtgaccta caccaacgtt attatgcaga gcgcggcgct gtataacgac
     3601 aacggcgagt tcatcggcat taccccggat tactatcgta agaaatacaa cgaagacacc
     3661 cgtttcgtga ccatgtttgt tcgtaaggac ggtaaacgta tcttcccgaa gagcttctgc
     3721 accgattata acgactttgt gggtaacggt accctggagc acccggcggg tggcgaacac
     3781 ctgaacaaca agctgaaccg taaaggtccg ggcttctttg cgctgggtac cccgttcacc
     3841 attatgccgg gccaagacat cgagattgat acctttaccg gtatgagcag cagcaaggat
     3901 aacccgaact acgatgacac caccatgccg aaagagctgg aagcgctgct gcgttatttc
     3961 gaagcgagcg atagcgtgca gagcgcgctg cgtgaggtta tcgacttcca agaaaagtac
     4021 tgcaagtaca tcgagttcaa gaccgaagac aagatgttca acgcgggttt taacaaaaac
     4081 ctggcgtttc agatcctgta ccaaaccttc atgagccgta gcttttgcca gacccaaaag
     4141 ggttatcgtg agatcggctt ccgtgaaatt caggatctgt acgcgagcat gtactacttc
     4201 atcaacctgg gttaccaaga cttcgtgaag aacctgctgt ttgagtggac cagcaacgtt
     4261 tacaaaatgg gctatgcgaa ccacaacttc tactgggttg gtaaacagcc gggcctgtat
     4321 agcgatgaca gcctgtggct gctgcaagcg tactatcgtt acatcattta taccaaggat
     4381 accagcatcc tgaacgagga agtgccggtt gcggatggta acaacgaaaa gcgtgcggtg
     4441 cgtgacaccc tgaaagcgat cattcagtac agcgcgttca tcagcgttgg taaccatggt
     4501 ctgccgctgc tggatctggc ggactggaac gattgcctga agattgataa cgactatgtg
     4561 gacggtgcgc agaaggagaa actgtactat aagcaactga aagaaaccgg tggcaaatac
     4621 ggcgatcgtt tcatgagcga ctatagcgag agcgttatga acgcgtttct gctgaaggtg
     4681 gcggttgatg acctggcgga gatcgcggaa ctggataagg acgcggagct ggaacagagc
     4741 atgcgtaaaa tcagcagcgc gctgagcgaa aagattcaaa aatacgcgtg gaagggtaac
     4801 ttctttgcgc gtgtgctgct gaaccgttac aaagatggca gcttcaccta tgttggtgcg
     4861 aagggcgaca aactgagcgc ggacccgaac atcgatggta cctatttcct gaacagcttt
     4921 agctgggcga ttctgagcgg tgtggcgacc gatgagcaga tcggcattat ggttgacacc
     4981 ctgaagaaat acatgctgac cccgtatggc atccgtctga ccaccccggc ggatctgaac
     5041 aaactggcga acgacaccgc gaccgatcac tacttctatg gtgaccgtga aaacggcgcg
     5101 gtgtttaagc atgcgagcat gatggcgacc gcggcgctgg cgaaggcggc gaaaagcgtt
     5161 aaggataaag cgctggcgaa agagatggcg cgtctggcgt actatatgat cgatctggtg
     5221 ctgccgtaca agaacctgga agacccgttc aaactggcgg gtaacccgcg tatctgcacc
     5281 cagtatatta acgcggacag cggcgagaac attggtccgc tgctgagcgg taccgcgacc
     5341 tggctgaacc tgaccatcac cagcctggcg ggtattgagt acgtgaagga tggcattatg
     5401 ttcagcccgg ttctgcgtga ggacgaaacc cgtctggaat acagcctgaa ggcgccgggt
     5461 tgcagctata acatcaccat taccaaaccg cgtggctttg cgcgtctgat cgacagcgcg
     5521 tacgatctgc gtattgacgg taagaaaacc gacaactgca ttatcccgat gttcaccgat
     5581 ggtaaagagc atagcgtgac cctggagttc aagtaacaat tgtgtacaaa agaggagaaa
     5641 acccccatgg caagctggag ccacccgcag ttcgaaaagg gtaccatgaa aaataaagtc
     5701 caactgatta cctatgcgga tcgtctgggt gatggcacca ttaaaagcat gacggacatc
     5761 ctgcgtaccc gctttgatgg cgtttatgac ggtgtccaca ttctgccgtt tttcaccccg
     5821 tttgatggcg ccgacgcagg tttcgatccg atcgaccata ccaaagttga tgaacgtctg
     5881 ggtagctggg atgacgtcgc cgaactgtct aaaacccaca acattatggt ggatgcaatc
     5941 gttaatcaca tgtcatggga atcgaaacag ttccaagatg ttctggccaa aggcgaagaa
     6001 tcagaatatt acccgatgtt tctgaccatg agcagcgtgt tcccgaacgg tgccacggaa
     6061 gaagacctgg caggcattta tcgtccgcgt ccgggtctgc cgtttaccca ctacaaattc
     6121 gcaggcaaaa cgcgtctggt ctgggtgtct tttaccccgc agcaagtgga catcgatacg
     6181 gacagtgata aaggttggga atatctgatg tccattttcg accaaatggc ggccagccat
     6241 gtgtcttata tccgcctgga tgcggttggc tacggtgcca aagaagcagg caccagttgc
     6301 tttatgaccc cgaaaacgtt caaactgatt tcccgtctgc gcgaagaagg cgtgaaacgc
     6361 ggtctggaaa ttctgatcga agtccacagc tattacaaaa aacaggtgga aatcgcgtct
     6421 aaagtggatc gcgtttatga ctttgccctg ccgccgctgc tgctgcatgc cctgagtacc
     6481 ggccacgtcg aaccggtggc ccattggacg gatattcgtc cgaacaatgc tgttaccgtc
     6541 ctggatacgc atgacggcat cggcgttatt gatatcggtt cagatcagct ggaccgctcg
     6601 ctgaaaggtc tggtgccgga cgaagatgtg gataacctgg tgaatacgat tcatgcgaac
     6661 acccacggcg aatcacaggc agctaccggt gcggcggcat cgaacctgga tctgtaccaa
     6721 gttaatagta cctactactc cgctctgggc tgtaacgatc agcactacat cgctgcgcgt
     6781 gcggtgcagt ttttcctgcc gggtgttccg caagtctatt acgtgggcgc cctggcaggt
     6841 aaaaatgata tggaactgct gcgcaaaacc aacaatggtc gtgacattaa ccgccattat
     6901 tacagcacgg ccgaaatcga tgaaaatctg aaacgtccgg tggttaaagc actgaacgct
     6961 ctggcgaaat ttcgcaatga actggatgct tttgacggca ccttctcata taccacggat
     7021 gacgatacga gtatttcctt tacctggcgt ggtgaaacgt cgcaggccac cctgacgttc
     7081 gaaccgaaac gcggcctggg tgttgataat accacgccgg tcgcaatgct ggaatgggaa
     7141 gacagtgctg gtgaccatcg ctccgacgac ctgatcgcta atccgccggt tgttgcgtaa
     7201 aagcttcaaa aaacccctca agacccgttt agaggcccca aggggttatg ctaggagctc
     7261 gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc
     7321 tcggttgccg ccgggcgttt tttattggtg agaatggatc cgttggcggg tgtcggggcg
     7381 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc
     7441 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
     7501 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     8041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     8101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     8161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     8221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     8281 aaaaggatct caagaagatc ctttgatctc tcgagtaaaa aaaatcctta gctttcgcta
     8341 aggatctgca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     8401 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     8461 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     8521 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     8581 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     8641 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     8701 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     8761 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     8821 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     8881 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     8941 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     9001 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     9061 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9121 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9181 ataagggcga cacggaaatg ttgaatactc atgcttaatt tctcctcttt aatagatctt
     9241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     9301 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag
     9361 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc
     9421 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa
     9481 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc
     9541 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc
     9601 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca
     9661 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc
     9721 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
     9781 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag
     9841 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
     9901 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
     9961 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
    10021 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
    10081 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
    10141 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
    10201 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
    10261 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
    10321 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
    10381 ttgcaccatt cgatggtgtc
//
