LOCUS       2376_to_3086_of_       13790 bp    DNA     circular SYN 19-Feb-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     source          join(<110..>402,382..>402,<406..>464,<406..>464)
                     /note=" [Split]"
     source          <465..489
     source          <465..489
                     /note=" [Split]"
     source          490..>494
                     /mol_type="other DNA"
                     /organism="pInducer10-mir-RUP-PheS"
                     /note=" [Split]"
     source          501..>1478
                     /mol_type="genomic DNA"
                     /organism="Macacine herpesvirus 3"
                     /country="USA"
                     /culture_collection="ATCC:VR-677"
                     /db_xref="taxon:47929"
                     /host="Macaca mulatta"
                     /isolation_source="urine from a rhesus macaque"
                     /note=" [Split]"
                     /strain="68-1"
     source          2205..>2227
                     /mol_type="other DNA"
                     /organism="pInducer10-mir-RUP-PheS"
                     /note=" [Split]"
     source          <2228..>2231
                     /mol_type="other DNA"
                     /organism="pInducer10-mir-RUP-PheS"
                     /note=" [Split]"
     promoter        complement(5..23)
                     /label="T7_promoter"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     frag            <89..93
                     /date="31-Dec-2015"
                     /note="1 to 25 of FWD2 TRE3G [Split]"
     frag            join(89..>402,<406..>464)
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /note="21 to 422 of TRE3G PCR PROD2 [Split]"
                     /right_cutter="AgeI (422)"
                     /right_end="5CCGG"
     frag            join(94..>402,<406..>464)
                     /date="31-Dec-2015"
                     /note="1081 to 1483 of VB plasmid Seq-corrected [Split]"
     frag            <94..109
                     /date="10-Dec-2015"
                     /note="1 to 291 of Untitled [Split]"
     frag            join(<110..>402,<406..>464)
                     /note=" [Split]"
     promoter        114..>464
                     /note="Tet-responsive Element 3G [Split]"
     misc_binding    118..137
                     /note="tet-operator"
     misc_binding    154..173
                     /note="tet-operator"
     misc_binding    190..209
                     /note="tet-operator"
     misc_binding    226..245
                     /note="tet-operator"
     misc_binding    262..281
                     /note="tet-operator"
     misc_binding    298..317
                     /note="tet-operator"
     misc_binding    334..353
                     /note="tet-operator"
     misc_feature    402..416
                     /note="crs (mutated)"
     frag            465..2227
                     /date="10-Oct-2018"
                     /note="1 to 1763 of PCR product 159_GFP for pOUPc Gibson"
     frag            <465..489
     promoter        <465..488
                     /note="Tet-responsive Element 3G [Split]"
     frag            <465..489
                     /note=" [Split]"
     frag            <465..489
                     /note=" [Split]"
     frag            <465..489
                     /note=" [Split]"
     frag            465..494
                     /date="01-Oct-2018"
                     /note="465 to 494 of pOUPc-RFP"
     frag            465..500
                     /date="10-Oct-2018"
                     /note="1 to 36 of Gibs_Rh159_Fw"
     frag            490..>494
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS plus 1600 to
                     866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     frag            490..>494
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="5CCGG"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS [Split]"
                     /right_cutter="NotI (3086)"
                     /right_end="5GGCC"
     CDS             complement(490..>494)
                     /label="ORF frame 1"
                     /note=" [Split]"
                     /translation="MCCRSEHLTRLAAASSVPQFAREVAVLGHSHLVLLDVGLFVGLLD
                     SFQSVVHEVEAGHLEVLSGFLGSVCGLEGAVQVAPAHELQGHLAVASQAAVSGVQHLGV
                     GLPAACFLLHHRAVGWEVHPVDLDVVDDAAVLEAGVLGSGQHAPVFVCGDSLPCEALRE
                     GLLKEVGDALGVVDEGFAAVHEAGSQDVEGEGERAALDHLDLHGLGALVGLAFALGCAL
                     EVVVVHGALHVQLHVHVLLDQLAHGGDR*"
     frag            495..>500
                     /date="18-Oct-2016"
                     /note="1 to 1054 of Rh159_HA PCR for Gibson EcoRV to
                     pEF1_pcDNA3.1 [Split]"
     frag            495..>500
                     /date="27-Aug-2018"
                     /note="945 to 982 of pcDNA3.1_+_Rh159_HA [Split]"
     frag            495..>500
                     /date="01-Oct-2018"
                     /note="22 to 42 of Gibs_159_Fw [Split]"
     frag            <498..500
                     /date="11-Oct-2016"
                     /note="374 to 401 of RH159-T7_G07.ab1"
     CDS             501..>1478
                     /codon_start=1
                     /db_xref="GI:51556614"
                     /db_xref="GeneID:2952701"
                     /locus_tag="grh159"
                     /note="similar to UL148 [Split]"
                     /product="rh159"
                     /protein_id="YP_068248.1"
                     /translation="MAYNSFLLSCLTIALLLHGLPTSTSAKDMAGYVHVHMLNAPEDAA
                     STSAPVIQLANGFITCRKFTELGWALCTSWDDMECFPFWRRTFSETKNITVLSDDPYYA
                     GVVLTVRTLLGDSLELTSYLGVRPSIANVGYYVMYLSYAKALPAKFTHNDVDYVRFAIY
                     LHTEIDAQQTPDNNFVFTFATPGTFETPDLMSSFVIGTDDGQNSTLNFTTVGGEKHRYN
                     NVELSSEMKGNCVYHSAIIRNVASSQSWVSFDFSLYWRRTKIAYSYVQYYFPKPLHSPE
                     PIGPAWQRIYHYFSLLTQLRYGTVAITVVLIFLIGALLKRSREAH"
     gene            501..>1478
                     /db_xref="GeneID:2952701"
                     /locus_tag="grh159"
                     /note=" [Split]"
     frag            501..>1478
                     /date="11-Oct-2016"
                     /note="164371 to 165351 of RhCMV CG [Split]"
     frag            501..1478
                     /date="01-Sep-2018"
                     /note="1 to 978 of Rh159 CDS"
     frag            501..2195
                     /date="10-Oct-2018"
                     /note="972 to 2666 of pcDNA3.1_Rh159_GFP"
     primer_bind     958..977
                     /note="Rh159_Seq 477 Fw"
     primer_bind     1449..1468
                     /note="Rh159_Seq 968 Fw"
     frag            1479..>2195
                     /note=" [Split]"
     CDS             1479..2195
                     /note="eGFP"
     frag            1479..2195
                     /date="10-Nov-2017"
                     /note="32 to 748 of EGFP-P2A-3XHA"
     frag            1479..>2195
                     /date="16-Nov-2017"
                     /note="21 to 759 of eGFP_PCR prod [Split]"
     frag            complement(<2196..2198)
                     /date="01-Oct-2018"
                     /note="20 to 46 of Gib_eGFP_Rv [Split]"
     frag            complement(<2196..2227)
                     /date="10-Oct-2018"
                     /note="1 to 54 of eGFP_Rv_Gibs [Split]"
     frag            2196..2227
                     /date="10-Oct-2018"
                     /note="23 to 54 of Untitled"
     misc_feature    2199..2204
                     /note="MluI added"
     frag            <2199..>2227
                     /date="27-Jun-2016"
                     /note="1 to 60 of ssDNA oligo [Split]"
     frag            2199..>2227
                     /date="01-Oct-2018"
                     /note="1201 to 1230 of pOUPc-RFP [Split]"
     frag            2205..>2227
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (866)"
                     /left_end="3GGCC"
                     /note="1600 to 866 of pINDv2 [Split]"
                     /right_cutter="NotI (1599)"
                     /right_end="3CCGG"
     misc_feature    2205..>2227
                     /note="oligo gibs [Split]"
     frag            2205..>2227
                     /date="12-Jan-2016"
                     /left_cutter="NheI (11678)"
                     /left_end="3GATC"
                     /left_join="keep end"
                     /note="21 to 422 of TRE3G PCR PROD2 plus 12148 to 11678 of
                     pINDUCER10 dTomato-mirE (STEP ONE) [Split]"
                     /right_cutter="NheI (20)"
                     /right_end="3GATC"
                     /right_join="keep end"
     frag            2205..>2227
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (866)"
                     /left_end="3GGCC"
                     /left_join="keep end"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS plus 1600 to
                     866 of pINDv2 [Split]"
                     /right_cutter="AgeI (2375)"
                     /right_end="3GGCC"
                     /right_join="keep end"
     frag            2205..>2227
                     /date="12-Jan-2016"
                     /left_cutter="MluI (1222)"
                     /left_end="3GCGC"
                     /left_join="keep end"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS plus 83 to
                     1222 of pSP72 dTomato_mirE [Split]"
                     /right_cutter="MluI (4795)"
                     /right_end="3GCGC"
                     /right_join="keep end"
     frag            2205..>2227
                     /date="12-Jan-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="3GGCC"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS [Split]"
                     /right_cutter="MluI (4795)"
                     /right_end="3GCGC"
     frag            2205..>2227
                     /date="12-Jan-2016"
                     /left_cutter="NheI (11678)"
                     /left_end="3GATC"
                     /note="12148 to 11678 of pINDUCER10 dTomato-mirE (STEP ONE)
                     [Split]"
                     /right_cutter="AgeI (12147)"
                     /right_end="3GGCC"
     frag            2205..>2227
                     /date="20-Jun-2016"
                     /note="1612 to 1641 of pOUP-RFP [Split]"
     promoter        2208..>2227
                     /label="hUbC_promoter"
                     /note="/translation=""MHHPAAQHFQPFTVFTHDVHFCRRFGKREVGRTEANL
                     QIFLKEV
                     TQEVVQRAFQVGKADVGINNQTFHLMEHRRVSLVVVVTINTARRDDTNRWLLGFHGAD
                     LYARGLGTQQAGSVKPESVVVSARRVVTRNVQSIEVMIIVFDFRPGCHGKAKLTEETF
                     DTVDGTGNRMQTAVFNATTWQRDINRFRRQTRIQCSAFQFRFTRIQRLLNLLFRFVDN
                     RTSCRTLFWRQLTQGRHLKG*"" [Split]"
     frag            <2228..2231
                     /date="27-Jun-2016"
                     /note="1 to 60 of ssDNA oligo"
     frag            <2228..>2231
                     /date="12-Jan-2016"
                     /left_cutter="MluI (4795)"
                     /left_end="5CGCG"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS [Split]"
                     /right_cutter="AgeI (2375)"
                     /right_end="5CCGG"
     frag            <2228..>2231
                     /date="12-Jan-2016"
                     /left_cutter="MluI (4795)"
                     /left_end="5CGCG"
                     /left_join="keep end"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS plus 83 to
                     1222 of pSP72 dTomato_mirE [Split]"
                     /right_cutter="MluI (1222)"
                     /right_end="5CGCG"
                     /right_join="keep end"
     frag            <2228..>2231
                     /date="19-Feb-2016"
                     /left_cutter="NotI (1599)"
                     /left_end="5GGCC"
                     /note="1600 to 866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
     misc_feature    <2228..>2231
                     /note="oligo gibs"
     frag            <2228..>2231
                     /date="20-Jun-2016"
                     /note="1612 to 1641 of pOUP-RFP"
     frag            <2228..>2231
                     /date="12-Jan-2016"
                     /left_cutter="AgeI (12147)"
                     /left_end="5CCGG"
                     /note="12148 to 11678 of pINDUCER10 dTomato-mirE (STEP ONE)
                     [Split]"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
     frag            <2228..>2231
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /left_join="keep end"
                     /note="21 to 422 of TRE3G PCR PROD2 plus 12148 to 11678 of
                     pINDUCER10 dTomato-mirE (STEP ONE) [Split]"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
                     /right_join="keep end"
     frag            <2228..>2231
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS plus 1600 to
                     866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     promoter        <2228..3424
                     /label="hUbC_promoter"
                     /note="/translation=""MHHPAAQHFQPFTVFTHDVHFCRRFGKREVGRTEANL
                     QIFLKEV
                     TQEVVQRAFQVGKADVGINNQTFHLMEHRRVSLVVVVTINTARRDDTNRWLLGFHGAD
                     LYARGLGTQQAGSVKPESVVVSARRVVTRNVQSIEVMIIVFDFRPGCHGKAKLTEETF
                     DTVDGTGNRMQTAVFNATTWQRDINRFRRQTRIQCSAFQFRFTRIQRLLNLLFRFVDN
                     RTSCRTLFWRQLTQGRHLKG*"" [Split]"
     frag            join(<2232..13790,<1..24)
                     /date="19-Feb-2016"
                     /left_cutter="NotI (1599)"
                     /left_end="5GGCC"
                     /note="1600 to 866 of pINDv2"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
     frag            join(<2232..>13790,<1..>24)
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /left_join="keep end"
                     /note="21 to 422 of TRE3G PCR PROD2 plus 12148 to 11678 of
                     pINDUCER10 dTomato-mirE (STEP ONE)"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
                     /right_join="keep end"
     frag            join(<406..>464,<2235..>13790,<1..>402)
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS plus 1600 to
                     866 of pINDv2"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     misc_feature    3406..3425
                     /note="gibs?"
     misc_feature    complement(3431..3464)
                     /label="loxP"
                     /note="/translation=""MHHPAAQHFQPFTVFTHDVHFCRRFGKREVGRTEANL
                     QIFLKEV
                     TQEVVQRAFQVGKADVGINNQTFHLMEHRRVSLVVVVTINTARRDDTNRWLLGFHGAD
                     LYARGLGTQQAGSVKPESVVVSARRVVTRNVQSIEVMIIVFDFRPGCHGKAKLTEETF
                     DTVDGTGNRMQTAVFNATTWQRDINRFRRQTRIQCSAFQFRFTRIQRLLNLLFRFVDN
                     RTSCRTLFWRQLTQGRHLKG*"""
     CDS             3444..4217
                     /label="ORF frame 3"
                     /translation="MYAIRSYATMSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGV
                     EQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRD
                     GAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVA
                     KEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALD
                     DFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"
     misc_feature    4218..4251
                     /label="loxP"
                     /note="/translation=""MYAIRSYATMSRLDKSKVINGALELLNGVGIEGLTTR
                     KLAQKLG
                     VEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSH
                     RDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEH
                     QVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPT
                     DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"""
     misc_feature    4266..4829
                     /label="IRES"
                     /note="/translation=""MYAIRSYATMSRLDKSKVINGALELLNGVGIEGLTTR
                     KLAQKLG
                     VEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSH
                     RDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEH
                     QVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPT
                     DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"""
     CDS             4842..5444
                     /label="ORF frame 3"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     gene            4857..5444
                     /gene="puro (variant)"
                     /label="puro (variant)"
                     /note="/translation=""MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHT
                     VDPDRHI
                     ERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGS
                     RLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF
                     LETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"""
     misc_feature    5445..5467
                     /note="gibs"
     misc_feature    5468..6043
                     /label="WPRE"
                     /note="/translation=""MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHT
                     VDPDRHI
                     ERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGS
                     RLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF
                     LETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"""
     CDS             5556..6083
                     /label="ORF frame 3"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFA
                     DATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLT
                     SFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWA
                     ASPPGINSAVET*"
     misc_feature    6261..6313
                     /label="delta_U3"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    6314..6494
                     /label="truncHIV-1_3_LTR"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    6314..6494
                     /label="HIV-1_5_LTR"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    complement(6595..6612)
                     /label="BGH_rev_primer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     regulatory      6598..6825
                     /regulatory_class="polyA_signal_sequence"
                     /note="BGH pA"
     terminator      6598..6825
                     /label="bGH_PA_terminator"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     rep_origin      6888..7194
                     /label="f1_origin"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    complement(7308..7328)
                     /label="pBABE_3_primer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    complement(7314..7529)
                     /label="SV40_enhancer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     promoter        7326..7594
                     /label="SV40_promoter"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     rep_origin      7493..7570
                     /label="SV40_origin"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    7555..7574
                     /label="SV40pro_F_primer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     gene            7721..8332
                     /gene="hygroB (420 - 1031)"
                     /label="hygroB (420 - 1031)"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     CDS             7805..8335
                     /label="ORF frame 2"
                     /translation="MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGR
                     ITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRI
                     GLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADS
                     GNRRPSTRPRAKE*"
     terminator      8468..8587
                     /label="SV40_PA_terminator"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     misc_feature    8556..8575
                     /label="EBV_rev_primer"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     promoter        complement(8631..8649)
                     /label="M13_reverse_primer"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     misc_feature    complement(8648..8670)
                     /label="M13_pUC_rev_primer"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     promoter        complement(8684..8713)
                     /label="lac_promoter"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     CDS             complement(9796..10656)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW*"
     gene            complement(9796..10656)
                     /gene="Ampicillin"
                     /label="Ampicillin"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     promoter        complement(10698..10726)
                     /label="AmpR_promoter"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     terminator      10812..10943
                     /label="SV40_PA_terminator"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     misc_feature    10900..10919
                     /label="EBV_rev_primer"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     misc_feature    11564..11744
                     /label="HIV-1_5_LTR"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     misc_feature    11564..11744
                     /label="truncHIV-1_3_LTR"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     CDS             complement(12181..12642)
                     /label="ORF frame 2"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPIV
                     LPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPPPG
                     LKISGRPLAVRWSYFCFALPLSCLKLPWCLLSLSFDAHNRGLLLYYIMI*"
     CDS             12347..13234
                     /label="ORF frame 3"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSALR
                     APILQTNGSIHPQF*"
     misc_feature    12469..12702
                     /label="RRE"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     misc_feature    13233..13248
                     /label="cPPT"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     misc_feature    complement(13411..13785)
                     /label="sh_ble"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     gene            complement(13414..13785)
                     /gene="bleo"
                     /label="bleo"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
BASE COUNT  3339 A  3519 C  3623 G  3309 T  0 OTHER
ORIGIN
        1 CCCTCCTATA GTGAGTCGTA TTATACTATG CCGATATACT ATGCCGATGA TTAATTGTCA
       61 ACACGTGCTG CAGGTCCGAG GTTCTAGGCT AGCGAGACTA GCGACGAGTT GGCTTTACTC
      121 CCTATCAGTG ATAGAGAACG TATGAAGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG
      181 CAGACTTTAC TCCCTATCAG TGATAGAGAA CGTATAAGGA GTTTACTCCC TATCAGTGAT
      241 AGAGAACGTA TGACCAGTTT ACTCCCTATC AGTGATAGAG AACGTATCTA CAGTTTACTC
      301 CCTATCAGTG ATAGAGAACG TATATCCAGT TTACTCCCTA TCAGTGATAG AGAACGTATA
      361 AGCTTTAGGC GTGTACGGTG GGCGCCTATA AAAGCAGAGC TCAGGTAGTG AACCGTCAGA
      421 TCGCCTGGAG CAATTCCACA ACACTTTTGT CTTATACCAA CTTTCCGTAC CACTTCCTAC
      481 CCTCGTAAAC CGGTgccACC ATGGCCTACA ACAGCTTCCT TCTCAGCTGC CTTACCATCG
      541 CACTTTTACT GCACGGTCTT CCAACATCAA CTAGCGCTAA AGACATGGCA GGCTACGTAC
      601 ACGTTCATAT GCTTAACGCA CCTGAAGACG CCGCTTCTAC GTCTGCGCCA GTTATCCAAC
      661 TAGCCAACGG CTTTATTACC TGCAGAAAAT TTACAGAGCT CGGTTGGGCA CTGTGTACTA
      721 GCTGGGATGA TATGGAGTGT TTTCCGTTTT GGCGAAGGAC TTTCTCGGAA ACTAAAAATA
      781 TCACTGTGCT TTCAGACGAT CCATACTATG CCGGCGTTGT GTTGACAGTA CGGACGCTTC
      841 TTGGTGACTC CCTAGAGCTT ACATCTTATC TCGGGGTCAG GCCTTCGATT GCCAATGTGG
      901 GCTACTATGT AATGTACCTG TCATATGCTA AGGCGCTCCC GGCAAAATTT ACCCACAACG
      961 ATGTGGACTA TGTGAGGTTT GCCATATACC TTCACACCGA AATTGATGCG CAGCAAACTC
     1021 CTGACAACAA CTTCGTCTTT ACGTTCGCCA CACCCGGGAC ATTTGAAACC CCAGACCTCA
     1081 TGAGTAGTTT TGTGATTGGA ACCGATGATG GACAAAATAG CACCTTGAAT TTCACCACTG
     1141 TTGGCGGAGA AAAACACCGC TACAACAACG TGGAGCTCAG CTCAGAAATG AAGGGCAATT
     1201 GTGTGTATCA CTCGGCTATT ATCCGGAACG TCGCCAGCTC GCAATCTTGG GTGTCGTTTG
     1261 ACTTCTCCCT ATATTGGCGA CGTACCAAAA TTGCATATAG TTACGTGCAG TATTATTTCC
     1321 CGAAACCACT CCATTCACCT GAACCCATAG GTCCTGCCTG GCAGAGAATC TACCATTACT
     1381 TTAGCCTCCT GACGCAGCTT CGCTACGGAA CAGTAGCGAT CACCGTGGTG CTCATTTTCC
     1441 TTATAGGTGC ACTTCTAAAA CGCAGTCGTG AAGCTCATAT GGTGAGCAAG GGGGAGGAGC
     1501 TTTTTACTGG CGTTGTTCCC ATCCTGGTTG AGTTGGATGG GGATGTCAAC GGCCACAAGT
     1561 TCAGCGTTTC TGGAGAAGGA GAAGGCGACG CAACCTATGG GAAGCTCACT TTGAAATTCA
     1621 TATGCACCAC CGGTAAACTC CCAGTCCCCT GGCCTACCCT GGTCACTACC TTGACATATG
     1681 GAGTTCAGTG CTTTTCTCGA TACCCCGACC ACATGAAGCA GCACGATTTT TTCAAATCTG
     1741 CAATGCCCGA GGGCTATGTA CAAGAGAGGA CCATTTTCTT CAAAGACGAC GGCAATTACA
     1801 AGACCAGGGC CGAGGTCAAA TTTGAAGGGG ACACTCTTGT GAATCGGATC GAACTTAAAG
     1861 GGATCGATTT CAAAGAGGAC GGAAACATAC TGGGGCATAA GCTGGAATAC AACTACAATA
     1921 GCCATAATGT TTACATTATG GCAGACAAGC AGAAAAACGG TATTAAGGTC AACTTCAAGA
     1981 TACGGCATAA CATTGAAGAT GGGTCAGTCC AGCTTGCTGA CCATTATCAG CAGAATACAC
     2041 CCATTGGCGA TGGTCCCGTG CTTTTGCCTG ACAACCACTA CCTTTCTACC CAAAGCGCAT
     2101 TGTCAAAAGA TCCTAACGAG AAGAGAGATC ATATGGTCCT TTTGGAGTTC GTAACCGCAG
     2161 CTGGTATAAC TCTGGGTATG GACGAGCTGT ATAAGtaaAC GCGTTAAGAT CTGGCCTCCG
     2221 CGCCGGGTTT TGGCGCCTCC CGCGGGCGCC CCCCTCCTCA CGGCGAGCGC TGCCACGTCA
     2281 GACGAAGGGC GCAGCGAGCG TCCTGATCCT TCCGCCCGGA CGCTCAGGAC AGCGGCCCGC
     2341 TGCTCATAAG ACTCGGCCTT AGAACCCCAG TATCAGCAGA AGGACATTTT AGGACGGGAC
     2401 TTGGGTGACT CTAGGGCACT GGTTTTCTTT CCAGAGAGCG GAACAGGCGA GGAAAAGTAG
     2461 TCCCTTCTCG GCGATTCTGC GGAGGGATCT CCGTGGGGCG GTGAACGCCG ATGATTATAT
     2521 AAGGACGCGC CGGGTGTGGC ACAGCTAGTT CCGTCGCAGC CGGGATTTGG GTCGCGGTTC
     2581 TTGTTTGTGG ATCGCTGTGA TCGTCACTTG GTGAGTAGCG GGCTGCTGGG CTGGCCGGGG
     2641 CTTTCGTGGC CGCCGGGCCG CTCGGTGGGA CGGAAGCGTG TGGAGAGACC GCCAAGGGCT
     2701 GTAGTCTGGG TCCGCGAGCA AGGTTGCCCT GAACTGGGGG TTGGGGGGAG CGCAGCAAAA
     2761 TGGCGGCTGT TCCCGAGTCT TGAATGGAAG ACGCTTGTGA GGCGGGCTGT GAGGTCGTTG
     2821 AAACAAGGTG GGGGGCATGG TGGGCGGCAA GAACCCAAGG TCTTGAGGCC TTCGCTAATG
     2881 CGGGAAAGCT CTTATTCGGG TGAGATGGGC TGGGGCACCA TCTGGGGACC CTGACGTGAA
     2941 GTTTGTCACT GACTGGAGAA CTCGGTTTGT CGTCTGTTGC GGGGGCGGCA GTTATGGCGG
     3001 TGCCGTTGGG CAGTGCACCC GTACCTTTGG GAGCGCGCGC CCTCGTCGTG TCGTGACGTC
     3061 ACCCGTTCTG TTGGCTTATA ATGCAGGGTG GGGCCACCTG CCGGTAGGTG TGCGGTAGGC
     3121 TTTTCTCCGT CGCAGGACGC AGGGTTCGGG CCTAGGGTAG GCTCTCCTGA ATCGACAGGC
     3181 GCCGGACCTC TGGTGAGGGG AGGGATAAGT GAGGCGTCAG TTTCTTTGGT CGGTTTTATG
     3241 TACCTATCTT CTTAAGTAGC TGAAGCTCCG GTTTTGAACT ATGCGCTCGG GGTTGGCGAG
     3301 TGTGTTTTGT GAAGTTTTTT AGGCACCTTT TGAAATGTAA TCATTTGGGT CAATATGTAA
     3361 TTTTCAGTGT TAGACTAGTA AATTGTCCGC TAAATTCTGG CCGTTTTTGG CTTTTTTGTT
     3421 AGACGGATCC ATAACTTCGT ATAATGTATG CTATACGAAG TTATGCCACC ATGTCTAGAC
     3481 TGGACAAGAG CAAAGTCATA AACGGAGCTC TGGAATTACT CAATGGTGTC GGTATCGAAG
     3541 GCCTGACGAC AAGGAAACTC GCTCAAAAGC TGGGAGTTGA GCAGCCTACC CTGTACTGGC
     3601 ACGTGAAGAA CAAGCGGGCC CTGCTCGATG CCCTGCCAAT CGAGATGCTG GACAGGCATC
     3661 ATACCCACTT CTGCCCCCTG GAAGGCGAGT CATGGCAAGA CTTTCTGCGG AACAACGCCA
     3721 AGTCATACCG CTGTGCTCTC CTCTCACATC GCGACGGGGC TAAAGTGCAT CTCGGCACCC
     3781 GCCCAACAGA GAAACAGTAC GAAACCCTGG AAAATCAGCT CGCGTTCCTG TGTCAGCAAG
     3841 GCTTCTCCCT GGAGAACGCA CTGTACGCTC TGTCCGCCGT GGGCCACTTT ACACTGGGCT
     3901 GCGTATTGGA GGAACAGGAG CATCAAGTAG CAAAAGAGGA AAGAGAGACA CCTACCACCG
     3961 ATTCTATGCC CCCACTTCTG AGACAAGCAA TTGAGCTGTT CGACCGGCAG GGAGCCGAAC
     4021 CTGCCTTCCT TTTCGGCCTG GAACTAATCA TATGTGGCCT GGAGAAACAG CTAAAGTGCG
     4081 AAAGCGGCGG GCCGACCGAC GCCCTTGACG ATTTTGACTT AGACATGCTC CCAGCCGATG
     4141 CCCTTGACGA CTTTGACCTT GATATGCTGC CTGCTGACGC TCTTGACGAT TTTGACCTTG
     4201 ACATGCTCCC CGGGTAAATA ACTTCGTATA GCATACATTA TACGAAGTTA TGGCGCGCCA
     4261 ATTCCGCCCC TCTCCCTCCC CCCCCCCTAA CGTTACTGGC CGAAGCCGCT TGGAATAAGG
     4321 CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG CCGTCTTTTG GCAATGTGAG
     4381 GGCCCGGAAA CCTGGCCCTG TCTTCTTGAC GAGCATTCCT AGGGGTCTTT CCCCTCTCGC
     4441 CAAAGGAATG CAAGGTCTGT TGAATGTCGT GAAGGAAGCA GTTCCTCTGG AAGCTTCTTG
     4501 AAGACAAACA ACGTCTGTAG CGACCCTTTG CAGGCAGCGG AACCCCCCAC CTGGCGACAG
     4561 GTGCCTCTGC GGCCAAAAGC CACGTGTATA AGATACACCT GCAAAGGCGG CACAACCCCA
     4621 GTGCCACGTT GTGAGTTGGA TAGTTGTGGA AAGAGTCAAA TGGCTCTCCT CAAGCGTATT
     4681 CAACAAGGGG CTGAAGGATG CCCAGAAGGT ACCCCATTGT ATGGGATCTG ATCTGGGGCC
     4741 TCGGTGCACA TGCTTTACAT GTGTTTAGTC GAGGTTAAAA AAACGTCTAG GCCCCCCGAA
     4801 CCACGGGGAC GTGGTTTTCC TTTGAAAAAC ACGATAATAC CATGGCCACC GAGTACAAGC
     4861 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCCGGGC CGTACGCACC CTCGCCGCCG
     4921 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGACCC GGACCGCCAC ATCGAGCGGG
     4981 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG
     5041 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG
     5101 CGGTGTTCGC CGAGATCGGC TCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC
     5161 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG
     5221 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC
     5281 CCGGAGTGGA GGCGGCCGAG CGCGCTGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC
     5341 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG
     5401 AAGGACCGCG CACCTGGTGC ATGACCCGCA AGCCCGGTGC CTGATTAATT AAGCGCGTCT
     5461 GGAACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG
     5521 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT
     5581 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG
     5641 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC
     5701 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC
     5761 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC
     5821 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA GCTGACGTCC TTTCCATGGC
     5881 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG
     5941 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC
     6001 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCCTGGAA
     6061 TTAATTCTGC AGTCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC
     6121 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT
     6181 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT
     6241 TTTAAAAGAA AAGAGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATCTGCT
     6301 TTTTGCTTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA
     6361 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG
     6421 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG
     6481 GAAAATCTCT AGCAGTAGTA GTTCATGTCA TCTTATTATT CAGTATTTAT AACTTGCAAA
     6541 GAAATGAATA TCAGAGAGTG AGAGGCCTTG ACATTGTTTA AACCCGCTGA TCAGCCTCGA
     6601 CTGTGCCTTC TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC
     6661 TGGAAGGTGC CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC
     6721 TGAGTAGGTG TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT
     6781 GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATGGCTTCT GAGGCGGAAA
     6841 GAACCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG
     6901 CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC
     6961 CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA
     7021 ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC
     7081 TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT
     7141 TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA
     7201 ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT
     7261 TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA ATTCTGTGGA ATGTGTGTCA
     7321 GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA AGTATGCAAA GCATGCATCT
     7381 CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA
     7441 AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC
     7501 CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA
     7561 TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT
     7621 TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA
     7681 TCAGCACGTG ATGAAAAAGC CTGAACTCAC CGCGACGTGA CCGCAAGGAA TCGGTCAATA
     7741 CACTACATGG CGTGATTTCA TATGCGCGAT TGCTGATCCC CATGTGTATC ACTGGCAAAC
     7801 TGTGATGGAC GACACCGTCA GTGCGTCCGT CGCGCAGGCT CTCGATGAGC TGATGCTTTG
     7861 GGCCGAGGAC TGCCCCGAAG TCCGGCACCT CGTGCACGCG GATTTCGGCT CCAACAATGT
     7921 CCTGACGGAC AATGGCCGCA TAACAGCGGT CATTGACTGG AGCGAGGCGA TGTTCGGGGA
     7981 TTCCCAATAC GAGGTCGCCA ACATCTTCTT CTGGAGGCCG TGGTTGGCTT GTATGGAGCA
     8041 GCAGACGCGC TACTTCGAGC GGAGGCATCC GGAGCTTGCA GGATCGCCGC GGCTCCGGGC
     8101 GTATATGCTC CGCATTGGTC TTGACCAACT CTATCAGAGC TTGGTTGACG GCAATTTCGA
     8161 TGATGCAGCT TGGGCGCAGG GTCGATGCGA CGCAATCGTC CGATCCGGAG CCGGGACTGT
     8221 CGGGCGTACA CAAATCGCCC GCAGAAGCGC GGCCGTCTGG ACCGATGGCT GTGTAGAAGT
     8281 ACTCGCCGAT AGTGGAAACC GACGCCCCAG CACTCGTCCG AGGGCAAAGG AATAGCACGT
     8341 GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT
     8401 CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA
     8461 CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT
     8521 CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT
     8581 ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA
     8641 GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG
     8701 CATAAAGTGT AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG
     8761 CTCACTGCCC GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA
     8821 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC
     8881 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG
     8941 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA
     9001 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA
     9061 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG
     9121 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT
     9181 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG
     9241 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC
     9301 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT
     9361 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA
     9421 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC
     9481 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC
     9541 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT
     9601 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC
     9661 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT
     9721 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA
     9781 AACTTGGTCT GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT
     9841 ATTTCGTTCA TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG
     9901 CTTACCATCT GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA
     9961 TTTATCAGCA ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT
    10021 ATCCGCCTCC ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT
    10081 TAATAGTTTG CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT
    10141 TGGTATGGCT TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT
    10201 GTTGTGCAAA AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC
    10261 CGCAGTGTTA TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC
    10321 CGTAAGATGC TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT
    10381 GCGGCGACCG AGTTGCTCTT GCCCGGCGTC CATACGGGAT AATACCGCGC CACATAGCAG
    10441 AACTTTAAAA GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT
    10501 ACCGCTGTTG AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC
    10561 TTTTACTTTC ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA
    10621 GGGAATAAGG GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG
    10681 AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA
    10741 TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TCGACGGATC
    10801 GGGAGATCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
    10861 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
    10921 ATGTATCTTA TCATGTCTGG ATCAACTGGA TAACTCAAGC TAACCAAAAT CATCCCAAAC
    10981 TTCCCACCCC ATACCCTATT ACCACTGCCA ATTACCTGTG GTTTCATTTA CTCTAAACCT
    11041 GTGATTCCTC TGAATTATTT TCATTTTAAA GAAATTGTAT TTGTTAAATA TGTACTACAA
    11101 ACTTAGTAGT TGGAAGGGCT AATTCACTCC CAAAGAAGAC AAGATATCCT TGATCTGTGG
    11161 ATCTACCACA CACAAGGCTA CTTCCCTGAT TAGCAGAACT ACACACCAGG GCCAGGGGTC
    11221 AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCC AGATAAGGTA
    11281 GAAGAGGCCA ATAAAGGAGA GAACACCAGC TTGTTACACC CTGTGAGCCT GCATGGGATG
    11341 GATGACCCGG AGAGAGAAGT GTTAGAGTGG AGGTTTGACA GCCGCCTAGC ATTTCATCAC
    11401 GTGGCCCGAG AGCTGCATCC GGAGTACTTC AAGAACTGCT GATATCGAGC TTGCTACAAG
    11461 GGACTTTCCG CTGGGGACTT TCCAGGGAGG CGTGGCCTGG GCGGGACTGG GGAGTGGCGA
    11521 GCCCTCAGAT CCTGCATATA AGCAGCTGCT TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA
    11581 CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA
    11641 AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA
    11701 GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGCCG CCCGAACAGG
    11761 GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA
    11821 GCGCGCACGG CAAGAGGCGA AGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC
    11881 GGAAGGCTAG AAGGAAGAAG ATGGTGCCAG AGCGTCAGTA TTAAGCGGGG GAAAATTAGA
    11941 TCGCGAAGGG AAAAAATTCG GTTAAGGCCA GGCCGAGTAA TACCGGGGGA GATTAGATCG
    12001 CGATGGGAAA AATTCGGTTA AGGCCAGGGG GAAAGAAAAA ATATAAATTA AAACATATAG
    12061 TATGGGCAAG CAGGGAGCTA GAACGATTCG CAGTTAATCC TGGCCTGTTA GAAACATCAG
    12121 AAGGCTGTAG ACAAATACTG GGACAGCTAC AACCATCCCT TCAGACAGGA TCAGAAGAAC
    12181 TTAGATCATT ATATAATACA GTAGCAACCC TCTATTGTGT GCATCAAAGG ATAGAGATAA
    12241 AAGACACCAA GGAAGCTTTA GACAAGATAG AGGAAGAGCA AAACAAAAGT AAGACCACCG
    12301 CACAGCAAGC GGCCGGCCGC TGATCTTCAG ACCTGGAGGA GGAGATATGA GGGACAATTG
    12361 GAGAAGTGAA TTATATAAAT ATAAAGTAGT AAAAATTGAA CCATTAGGAG TAGCACCCAC
    12421 CAAGGCAAAG AGAAGAGTGG TGCAGAGAGA AAAAAGAGCA GTGGGAATAG GAGCTTTGTT
    12481 CCTTGGGTTC TTGGGAGCAG CAGGAAGCAC TATGGGCGCA GCGTCAATGA CGCTGACGGT
    12541 ACAGGCCAGA CAATTATTGT CTGGTATAGT GCAGCAGCAG AACAATTTGC TGAGGGCTAT
    12601 TGAGGCGCAA CAGCATCTGT TGCAACTCAC AGTCTGGGGC ATCAAGCAGC TCCAGGCAAG
    12661 AATCCTGGCT GTGGAAAGAT ACCTAAAGGA TCAACAGCTC CTGGGGATTT GGGGTTGCTC
    12721 TGGAAAACTC ATTTGCACCA CTGCTGTGCC TTGGAATGCT AGTTGGAGTA ATAAATCTCT
    12781 GGAACAGATT TGGAATCACA CGACCTGGAT GGAGTGGGAC AGAGAAATTA ACAATTACAC
    12841 AAGCTTAATA CACTCCTTAA TTGAAGAATC GCAAAACCAG CAAGAAAAGA ATGAACAAGA
    12901 ATTATTGGAA TTAGATAAAT GGGCAAGTTT GTGGAATTGG TTTAACATAA CAAATTGGCT
    12961 GTGGTATATA AAATTATTCA TAATGATAGT AGGAGGCTTG GTAGGTTTAA GAATAGTTTT
    13021 TGCTGTACTT TCTATAGTGA ATAGAGTTAG GCAGGGATAT TCACCATTAT CGTTTCAGAC
    13081 CCACCTCCCA ACCCCGAGGG GACCCGACAG GCCCGAAGGA ATAGAAGAAG AAGGTGGAGA
    13141 GAGAGACAGA GACAGATCCA TTCGATTAGT GAACGGATCG GCACTGCGTG CGCCAATTCT
    13201 GCAGACAAAT GGCAGTATTC ATCCACAATT TTAAAAGAAA AGGGGGGATT GGGGGGTACA
    13261 GTGCAGGGGA AAGAATAGTA GACATAATAG CAACAGACAT ACAAACTAAA GAATTACAAA
    13321 AACAAATTAC AAAAATTCAA AATTTTCGGG TTTATTACAG GGACAGCAGA GATCCAGTTT
    13381 GGTTAGTACC GGGCCCGCTC TAGTCCGGAA TCAGTCCTGC TCCTCGGCCA CGAAGTGCAC
    13441 GCAGTTGCCG GCCGGGTCGC GCAGGGCGAA CTCCCGCCCC CACGGCTGCT CGCCGATCTC
    13501 GGTCATGGCC GGCCCGGAGG CGTCCCGGAA GTTCGTGGAC ACGACCTCCG ACCACTCGGC
    13561 GTACAGCTCG TCCAGGCCGC GCACCCACAC CCAGGCCAGG GTGTTGTCCG GCACCACCTG
    13621 GTCCTGGACC GCGCTGATGA ACAGGGTCAC GTCGTCCCGG ACCACACCGG CGAAGTCGTC
    13681 CTCCACGAAG TCCCGGGAGA ACCCGAGCCG GTCGGTCCAG AACTCGACCG CTCCGGCGAC
    13741 GTCGCGCGCG GTGAGCACCG GAACGGCACT GGTCAACTTG GCCATGGTGG
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