LOCUS       HIS_TEV_hsSYX3        6499 bp    DNA     circular     17-NOV-2021
DEFINITION  
SOURCE      
  ORGANISM  
COMMENT     SUMMARY:
            E. coli expression clone (T7lac promoter) for expression-optimized DNA of human 
            Syx (aa 393-792) with N-terminal His6 + TEV protease cleavage site.
            DETAILS:
            -	Protein sequence is MGSS + HHHHHH + GGG + TEV protease cleavage 
            (ENLYFQ*S; * = cleavage site) + human Syx (synectin-binding guanine exchange 
            factor; aa 393-792 from accession number NP_001036128.1).
            -	Confers kanamycin-resistance.
            -	Backbone vector is pET-28a.
            Construct design by Ryan Boyd. 
FEATURES             Location/Qualifiers
     promoter        4984..5000
                     /vntifkey="30"
                     /label=T7\promoter
     protein_bind    6343..6360
                     /vntifkey="31"
                     /label=His\tag
     terminator      6428..6474
                     /vntifkey="43"
                     /label=T7\terminator
     CDS             complement(3518..4597)
                     /vntifkey="4"
                     /label=lac\I
     rep_origin      2084..2084
                     /vntifkey="33"
                     /label=ColE1\pBR322\origin
     CDS             complement(563..1375)
                     /vntifkey="4"
                     /label=kan\sequence
     rep_origin      12..467
                     /vntifkey="33"
                     /label=f1\origin
     CDS             5003..5027
                     /vntifkey="4"
                     /label=lac\operator
     misc_feature    complement(2515..2706)
                     /vntifkey="21"
                     /label=rop
     RBS             5055..5064
                     /vntifkey="32"
                     /label=RBS
     CDS             5083..5100
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /vntifkey="4"
                     /label=6xHis
     CDS             5110..5130
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /vntifkey="4"
                     /label=TEV\site
     CDS             5131..6330
                     /vntifkey="4"
                     /label=aa393-792\of\HsSyx\NP_001036128.1,\optimized\for\E.\coli
     CDS             5071..6330
                     /vntifkey="4"
                     /label=EXPRESSED\PROTEIN
     rep_origin      1471..2144
                     /vntifkey="33"
                     /label=pBR322\origin\(pMB1,\ColE1)
     primer          4984..5003
                     /vntifkey="27"
                     /label=PRIMER\T7
     primer          complement(6413..6431)
                     /vntifkey="27"
                     /label=PRIMER\T7TERM
BASE COUNT     1538 a      1719 c      1751 g      1491 t 
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg 
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc 
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg 
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc 
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt 
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc 
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta 
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt 
      481 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta 
      541 tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac tgcaatttat 
      601 tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa 
      661 actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc 
      721 gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga 
      781 aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc atttctttcc 
      841 agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac 
      901 cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac 
      961 aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat 
     1021 tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg gggatcgcag 
     1081 tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca 
     1141 taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac 
     1201 ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat cgatagattg 
     1261 tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa tcagcatcca 
     1321 tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg ctcataacac 
     1381 cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa aatcccttaa 
     1441 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 
     1501 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 
     1561 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 
     1621 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 
     1681 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 
     1741 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 
     1801 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 
     1861 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 
     1921 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 
     1981 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 
     2041 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 
     2101 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 
     2161 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc 
     2221 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg 
     2281 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta 
     2341 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg 
     2401 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct 
     2461 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag 
     2521 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc 
     2581 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag 
     2641 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt 
     2701 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa 
     2761 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg 
     2821 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg 
     2881 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc 
     2941 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta 
     3001 cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca 
     3061 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc 
     3121 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc 
     3181 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa 
     3241 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc 
     3301 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac 
     3361 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca 
     3421 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta 
     3481 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 
     3541 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 
     3601 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca 
     3661 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa 
     3721 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt 
     3781 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg 
     3841 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca 
     3901 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta 
     3961 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg 
     4021 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat 
     4081 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct 
     4141 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg 
     4201 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat 
     4261 tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc 
     4321 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca 
     4381 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg 
     4441 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt 
     4501 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg 
     4561 catactctgc gacatcgtat aacgttactg gtttcacatt caccaccctg aattgactct 
     4621 cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg ccattcgatg gtgtccggga 
     4681 tctcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg 
     4741 ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc 
     4801 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg 
     4861 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg 
     4921 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga 
     4981 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa 
     5041 ttttgtttaa ctttaagaag gagatatacc atgggtagca gccatcatca tcatcatcac 
     5101 ggcggcggcg aaaacctgta cttccaaagc caacaagagg cggtgtggga actgctgcac 
     5161 accgaggcga gctacatccg taagctgcgt gtgatcatta acctgttcct gtgctgcctg 
     5221 ctgaacctgc aggaaagcgg cctgctgtgc gaggttgaag cggagcgtct gtttagcaac 
     5281 atcccggaga ttgcgcaact gcatcgtcgt ctgtgggcga gcgtgatggc gccggttctg 
     5341 gaaaaagcgc gtcgtacccg tgcgctgctg cagccgggtg acttcctgaa gggcttcaaa 
     5401 atgtttggta gcctgtttaa accgtacatc cgttattgca tggaggaaga gggctgcatg 
     5461 gagtacatgc gtggtctgct gcgtgacaac gacctgttcc gtgcgtatat tacctgggcg 
     5521 gaaaagcacc cgcagtgcca acgtctgaaa ctgagcgata tgctggcgaa gccgcaccaa 
     5581 cgtctgacca aatatccgct gctgctgaaa agcgtgctgc gtaagaccga agagccgcgt 
     5641 gcgaaagaag cggtggttgc gatgatcggc agcgtggagc gtttcattca ccacgttaac 
     5701 gcgtgcatgc gtcagcgtca agaacgtcag cgtctggcgg cggtggttag ccgtatcgac 
     5761 gcgtacgaag tggttgagag cagcagcgac gaagttgata agctgctgaa agagtttctg 
     5821 cacctggatc tgaccgcgcc gattccgggt gcgagcccgg aagaaacccg tcaactgctg 
     5881 ctggaaggca gcctgcgtat gaaagagggt aaagacagca agatggatgt gtattgcttc 
     5941 ctgtttaccg acctgctgct ggtgaccaag gcggttaaga aagcggaacg tacccgtgtt 
     6001 atccgtccgc cgctgctggt tgacaaaatt gtttgccgtg aactgcgtga tccgggcagc 
     6061 ttcctgctga tctacctgaa cgagtttcac agcgcggtgg gtgcgtatac ctttcaggcg 
     6121 agcggtcaag cgctgtgccg tggttgggtt gacaccattt acaacgcgca gaaccaactg 
     6181 caacaactgc gtgcgcagga accgccgggt agccagcaac cgctgcagag cctggaagag 
     6241 gaagaggatg agcaagaaga ggaagaggaa gaggaagaag aggaagaaga aggcgaagac 
     6301 agcggcacca gcgcggcgag cagcccgacc taataactcg agcaccacca ccaccaccac 
     6361 tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag 
     6421 caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa 
     6481 ggaggaacta tatccggat 
//
