LOCUS       MBP-g0s2        5994 bp    DNA     circular     17-NOV-2021
SOURCE      
  ORGANISM  
COMMENT     SUMMARY:
            E. coli expression clone (Ptac promoter) for full-length human G0S2 with N-terminal 
            mature maltose binding protein + enterokinase cleavage site.
            DETAILS:
            -	Protein sequence is the mature maltose binding protein (identical to aa 1-367 
            of accession no. 6K7E_A) + NSSSNNNNNNNNNNLG + enterokinase cleavage site 
            (DDDDK*; * = cleavage site) + VPH + human G0S2 (G0/G1 switch protein 2; accession 
            number NP_056529.1) + L.
            -	Confers ampicillin-resistance.
            -	This plasmid has been referred to as MBP g0s2, MBP-g0s2, 
            g0s2-noHis_pMAL-c5E, or pHis6-MBP-g0s2. There is no His-tag in the expressed 
            protein sequence.
            Construct design by James Zook.
FEATURES             Location/Qualifiers
     CDS             81..1163
                     /vntifkey="4"
                     /label=lacIq
     primer          2587..2610
                     /vntifkey="27"
                     /label=PRIMER\pMALFOR
     -35_signal      15..19
                     /vntifkey="48"
                     /label=lacIq\-35
     -10_signal      40..46
                     /vntifkey="47"
                     /label=lacIq\-10
     -35_signal      1406..1411
                     /vntifkey="48"
                     /label=Ptac\-35
     -10_signal      1428..1433
                     /vntifkey="47"
                     /label=Ptac\-10
     CDS             1528..3012
                     /vntifkey="4"
                     /label=EXPRESSED\PROTEIN\SEQUENCE
     terminator      3080..3166
                     /vntifkey="43"
                     /label=rrnB\T1\transcription\terminator
     primer          complement(3140..3163)
                     /vntifkey="27"
                     /label=PRIMER\pMALREV
     primer          2602..2625
                     /vntifkey="27"
                     /label=PRIMER\MBP-F
     terminator      3258..3285
                     /vntifkey="43"
                     /label=rrnB\T2\transcription\terminator
     -35_signal      3334..3339
                     /vntifkey="48"
                     /label=Amp-R\-35
     -10_signal      3357..3362
                     /vntifkey="47"
                     /label=Amp-R\-10
     CDS             3404..4261
                     /vntifkey="4"
                     /label=Amp-R
     rep_origin      4352..4940
                     /vntifkey="33"
                     /label=pMB1\origin\of\replication
     CDS             complement(5310..5501)
                     /vntifkey="4"
                     /label=rop
     CDS             2827..3009
                     /vntifkey="4"
                     /label=G0S2\(NP_056529.1)
     CDS             1531..2628
                     /vntifkey="4"
                     /label=MBP
     CDS             2677..2691
                     /vntifkey="4"
                     /label=enterokinase\cleavage\site
     promoter        3..80
                     /vntifkey="30"
                     /label=lacIq\promoter
     promoter        1442..1458
                     /vntifkey="30"
                     /label=lac\operator\(lacO)
     promoter        1406..1434
                     /vntifkey="30"
                     /label=tac\promoter
     RBS             3394..3398
                     /vntifkey="32"
                     /label=RBS
     promoter        3313..3403
                     /vntifkey="30"
                     /label=Amp-R\promoter
BASE COUNT     1494 a      1506 c      1635 g      1359 t 
ORIGIN
        1 ccgacaccat cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga 
       61 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg 
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa 
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac 
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc 
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg 
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc 
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca 
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga 
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc 
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg 
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag 
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga 
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa 
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatttcggta gtgggatacg 
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc 
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga 
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata 
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt 
     1141 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag 
     1201 gcacaattct catgtttgac agcttatcat cgactgcacg gtgcaccaat gcttctggcg 
     1261 tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc gtaaatcact gcataattcg 
     1321 tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt gcgccgacat cataacggtt 
     1381 ctggcaaata ttctgaaatg agctgttgac aattaatcat cggctcgtat aatgtgtgga 
     1441 attgtgagcg gataacaatt tcacacagga aacagccagt ccgtttaggt gttttcacga 
     1501 gcaattgacc aacaaggacc atagattatg aaaatcgaag aaggtaaact ggtaatctgg 
     1561 attaacggcg ataaaggcta taacggtctc gctgaagtcg gtaagaaatt cgagaaagat 
     1621 accggaatta aagtcaccgt tgagcatccg gataaactgg aagagaaatt cccacaggtt 
     1681 gcggcaactg gcgatggccc tgacattatc ttctgggcac acgaccgctt tggtggctac 
     1741 gctcaatctg gcctgttggc tgaaatcacc ccggacaaag cgttccagga caagctgtat 
     1801 ccgtttacct gggatgccgt acgttacaac ggcaagctga ttgcttaccc gatcgctgtt 
     1861 gaagcgttat cgctgattta taacaaagat ctgctgccga acccgccaaa aacctgggaa 
     1921 gagatcccgg cgctggataa agaactgaaa gcgaaaggta agagcgcgct gatgttcaac 
     1981 ctgcaagaac cgtacttcac ctggccgctg attgctgctg acgggggtta tgcgttcaag 
     2041 tatgaaaacg gcaagtacga cattaaagac gtgggcgtgg ataacgctgg cgcgaaagcg 
     2101 ggtctgacct tcctggttga cctgattaaa aacaaacaca tgaatgcaga caccgattac 
     2161 tccatcgcag aagctgcctt taataaaggc gaaacagcga tgaccatcaa cggcccgtgg 
     2221 gcatggtcca acatcgacac cagcaaagtg aattatggtg taacggtact gccgaccttc 
     2281 aagggtcaac catccaaacc gttcgttggc gtgctgagcg caggtattaa cgccgccagt 
     2341 ccgaacaaag agctggcaaa agagttcctc gaaaactatc tgctgactga tgaaggtctg 
     2401 gaagcggtta ataaagacaa accgctgggt gccgtagcgc tgaagtctta cgaggaagag 
     2461 ttggtgaaag atccgcgtat tgccgccact atggaaaacg cccagaaagg tgaaatcatg 
     2521 ccgaacatcc cgcagatgtc cgctttctgg tatgccgtgc gtactgcggt gatcaacgcc 
     2581 gccagcggtc gtcagactgt cgatgaagcc ctgaaagacg cgcagactaa ttcgagctcg 
     2641 aacaacaaca acaataacaa taacaacaac ctcggggatg acgatgacaa ggtaccgcat 
     2701 atggagaccg tgcaagaact gattccgctg gcgaaagaga tgatggcgca gaaacgcaag 
     2761 ggcaaaatgg tgaaactgta tgtgctgggt agcgtgctgg cgctgttcgg tgtggttctg 
     2821 ggcctgatgg aaaccgtttg cagcccgttt accgcggcgc gtcgtctgcg tgaccaggaa 
     2881 gcggcggtgg cggaactgca ggcggcgctg gagcgtcaag cgctgcagaa gcaagcgctg 
     2941 caagaaaaag gcaagcaaca agataccgtt ctgggtggcc gtgcgctgag caaccgtcaa 
     3001 catgcgagcc tgtaataacc atgggcggcc gcgatatcgt cgacggatcc gaattccctg 
     3061 caggtaatta aataagcttc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 
     3121 ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc cgggagcgga 
     3181 tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc 
     3241 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac 
     3301 tctttcggtc cgttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     3361 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     3421 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     3481 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     3541 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gtttcccaat 
     3601 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca 
     3661 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     3721 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     3781 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     3841 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     3901 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     3961 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     4021 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     4081 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     4141 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     4201 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     4261 gtaactgtca gaccaagttt actcatatat actttagatt gatttcctta ggactgagcg 
     4321 tcaaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 
     4381 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 
     4441 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 
     4501 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 
     4561 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 
     4621 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 
     4681 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 
     4741 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 
     4801 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 
     4861 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 
     4921 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 
     4981 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 
     5041 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 
     5101 cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg 
     5161 gtatttcaca ccgcatataa ggtgcactgt gactgggtca tggctgcgcc ccgacacccg 
     5221 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 
     5281 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 
     5341 gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgca gcgattcaca gatgtctgcc 
     5401 tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata 
     5461 aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg 
     5521 ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg 
     5581 gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta 
     5641 tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca 
     5701 gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg 
     5761 gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt 
     5821 catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt 
     5881 atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac 
     5941 gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga aatt 
//
