LOCUS       pCAG-NLS-dCas9-NLS-FLAG-p65(MLM)_(BPK1160)        9925 bp    DNA     circular     27-SEP-2014
DEFINITION  .
ACCESSION   urn.local...13y-e5h66x4
KEYWORDS    .
SOURCE      
  ORGANISM  .
COMMENT     Serial Cloner Genbank Format SerialCloner_Type=DNA
            SerialCloner_Comments=Gibson assembly into AgeI-HF/NotI-HF digested
            pCAG-CFP backb one. - - SerialCloner_Ends=0,0,,0,.
FEATURES             Location/Qualifiers
     misc_feature    18..1718
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label="CAG promoter"
     misc_feature    18..381
                     /SerialCloner_Color="&h03C000"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label="CMV Enhancer"
     misc_feature    84..371
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""CAG_enhancer"" - 84..371 \"
                     /label="CAG_enhancer"
     misc_feature    662..1625
                     /SerialCloner_Color="&hC01400"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label="Chicken beta-actin intron"
     primer_bind     1604..1624
                     /Sequence="CTGGCGTGTGACCGGCGGCTC"
                     /Hairpin_Tm="37.8"
                     /Tm="70.6"
                     /Self_Dimer_Tm="21.7"
                     /%GC="76.2"
                     /created_by="primer3"
                     /label="oBK303"
     misc_feature    1626..1718
                     /SerialCloner_Color="&hA304C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /label="Rabbit globin intron"
     primer_bind     1664..1683
                     /Sequence="CCTACAGCTCCTGGGCAACG"
                     /Transferred_From="oBK280"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    1678..1697
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""pCAG_F_primer"" -
                     translation=""MFMPSSFSYSSWATCWLLCCLIILAKNSAVDGTAGPGSTGRHHG
                     -
                     EQGRGAVHRGGAHPGRAGRRRKRPQVQRVRRGRGRCHLRQADPEVHLHHRQAARALAH
                     -
                     PRDHPDLRRAVLQPLPRPHEAARLLQVRHARRLRPGAHHLLQGRRQLQDPRRGEVRGR
                     - HPGEPHRAEGHRLQGGRQHPGAQAGVQLQQPQRLYHGRQAEERHQGELQ \"
                     /label="pCAG_F_primer"
     primer_bind     1678..1697
                     /Sequence="GCAACGTGCTGGTTATTGTG"
                     /Transferred_From="SQT367"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     1724..1745
                     /Sequence="CTGCAGTCGACGGTACCGCGGG"
                     /Transferred_From="oBK91"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     1724..1743
                     /Sequence="CTGCAGTCGACGGTACCGCG"
                     /Transferred_From="oBK597"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    1736..2078
                     /created_by="User"
                     /modified_by="User"
                     /label="gBlock oBK360"
     misc_feature    1736..1760
                     /created_by="User"
                     /label="Gibson overlap"
     motif           1763..1772
                     /SerialCloner_Color="&hB400C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="note=""direct strand translation"" \"
                     /SerialCloner_Desc="translation=""AT"" \"
                     /modified_by="User"
                     /label="Kozak sequence"
     ORF             <1772..1798
                     /created_by="User"
                     /modified_by="User"
                     /label="SV40NLS"
     misc_feature    1780
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1783
                     /Original_Bases="G"
                     /label="G"
                     /note="Geneious type: Editing History Replacement"
     misc_feature    1831^1832
                     /Original_Bases="ATG"
                     /label="ATG"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    <1832..>6031
                     /SerialCloner_Color="&h0031C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="note=""direct strand translation"" \"
                     /SerialCloner_Desc="translation=""MDKKYSIGLDIGTNSVGWAVITDE
                     YKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYL
                     QEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRK
                     KLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEE
                     NPINASGVDAK \"
                     /Color="#ff0000"
                     /modified_by="User"
                     /label="dCas9"
     primer_bind     complement(1861..1884)
                     /Sequence="GCCCATCCAACGGAATTAGTGCCG"
                     /Transferred_From="oBK603"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     1861..1882
                     /Sequence="CGGCACTAATTCCGTTGGATGG"
                     /Transferred_From="oBK393"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     2017..2035
                     /Sequence="CCTGAAACGAACCGCTCGG"
                     /Transferred_From="oBK237"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(2040..2075)
                     /Sequence="GTAAGTAACATATTCGGTTCTTGCGACGTGTATACC"
                     /Transferred_From="oBK596"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(2046..2071)
                     /Sequence="GTAACATATTCGGTTCTTGCGACGTG"
                     /Hairpin_Tm="None"
                     /Tm="62.8"
                     /Self_Dimer_Tm="None"
                     /%GC="46.2"
                     /created_by="primer3"
                     /label="oBK89"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(2046..2071)
                     /Sequence="GTAACATATTCGGTTCTTGCGACGTG"
                     /Transferred_From="oBK89"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(2088..2111)
                     /Sequence="CGTCAACTTTGGCCATCTCATTGC"
                     /Transferred_From="oBK93"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     2261..2269
                     /created_by="User"
                     /label="Loop A"
     primer_bind     2317..2348
                     /Sequence="GTTCCGTGGGCACTTTCTCATTGAGGGTGATC"
                     /Transferred_From="oBK580"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(2325..2348)
                     /Sequence="GATCACCCTCAATGAGAAAGTGCC"
                     /Hairpin_Tm="30.7"
                     /Tm="61.7"
                     /Self_Dimer_Tm="11.0"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="oBK420"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2325..2348
                     /Sequence="GGCACTTTCTCATTGAGGGTGATC"
                     /Transferred_From="JS4588"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(2325..2348)
                     /Sequence="GATCACCCTCAATGAGAAAGTGCC"
                     /Transferred_From="oBK420"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_difference 2351..2749
                     /created_by="User"
                     /label="delta 175-307"
     sig_peptide     2351..2359
                     /created_by="User"
                     /label="Loop B"
     misc_feature    complement(2364..2387)
                     /Sequence="GGATGAACAGTTTGTCGACATCCG"
                     /Hairpin_Tm="41.1"
                     /Tm="62.2"
                     /Self_Dimer_Tm="18.7"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="oBK422"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2364..2387
                     /Sequence="CGGATGTCGACAAACTGTTCATCC"
                     /Hairpin_Tm="42.7"
                     /Tm="62.2"
                     /Self_Dimer_Tm="21.4"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="oBK421"
     primer_bind     complement(2364..2387)
                     /Sequence="GGATGAACAGTTTGTCGACATCCG"
                     /Transferred_From="oBK422"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     2423..2431
                     /created_by="User"
                     /label="Loop C"
     misc_feature    complement(2471..2490)
                     /Sequence="CGTCGGGATTTAGAGAGGCG"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /label="oBK424"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2471..2490
                     /Sequence="CGCCTCTCTAAATCCCGACG"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="60.0"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="oBK423"
     primer_bind     complement(2471..2490)
                     /Sequence="CGTCGGGATTTAGAGAGGCG"
                     /Transferred_From="oBK424"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     2513..2521
                     /created_by="User"
                     /label="Loop D"
     primer_bind     2918..2950
                     /Sequence="GACGGCGGAGCGAGTCAAGAGGAATTCTACAAG"
                     /Transferred_From="oBK581"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(2922..2944)
                     /Sequence="GAATTCCTCTTGACTCGCTCCGC"
                     /Hairpin_Tm="None"
                     /Tm="63.6"
                     /Self_Dimer_Tm="None"
                     /%GC="56.5"
                     /created_by="primer3"
                     /label="oBK425"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2922..2944
                     /Sequence="GCGGAGCGAGTCAAGAGGAATTC"
                     /Transferred_From="JS4589"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(2922..2944)
                     /Sequence="GAATTCCTCTTGACTCGCTCCGC"
                     /Transferred_From="oBK425"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(3263..3293)
                     /Sequence="GAGCTGACGCACCTTTATCGACAACTTCCTC"
                     /Transferred_From="oBK586"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     3263..3293
                     /Sequence="GAGGAAGTTGTCGATAAAGGTGCGTCAGCTC"
                     /Transferred_From="oBK585"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     3425..3433
                     /created_by="User"
                     /label="Loop E"
     primer_bind     3473..3509
                     /Sequence="GTAGATCTGTTATTCAAGACCAACCGCAAAGTGACAG"
                     /Transferred_From="oBK582"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(3487..3507)
                     /Sequence="GTCACTTTGCGGTTGGTCTTG"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="oBK427"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3487..3507
                     /Sequence="CAAGACCAACCGCAAAGTGAC"
                     /Hairpin_Tm="None"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="52.4"
                     /created_by="primer3"
                     /label="oBK426"
     primer_bind     complement(3487..3507)
                     /Sequence="GTCACTTTGCGGTTGGTCTTG"
                     /Transferred_From="oBK427"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     3542..3550
                     /created_by="User"
                     /label="Loop F"
     primer_bind     3554..3585
                     /Sequence="GATTCTGTCGAGATCTCCGGGGTAGAAGATCG"
                     /Transferred_From="oBK583"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(3562..3584)
                     /Sequence="GATCTTCTACCCCGGAGATCTCG"
                     /Hairpin_Tm="None"
                     /Tm="61.4"
                     /Self_Dimer_Tm="17.2"
                     /%GC="56.5"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="oBK302"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     3562..3584
                     /Sequence="CGAGATCTCCGGGGTAGAAGATC"
                     /Transferred_From="JS4590"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(3562..3584)
                     /Sequence="GATCTTCTACCCCGGAGATCTCG"
                     /Transferred_From="oBK302"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     3640..3661
                     /Sequence="GGACTTCCTGGATAACGAAGAG"
                     /Hairpin_Tm="None"
                     /Tm="58.0"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /label="oBK430"
     primer_bind     complement(3673..3703)
                     /Sequence="GAGGGTAAGAGTCAACACTATATCTTCTAAG"
                     /Transferred_From="oBK240"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(3686..3716)
                     /Sequence="CCCGATCTTCAAAGAGGGTAAGAGTCAACAC"
                     /Transferred_From="oBK587"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     3842..3850
                     /created_by="User"
                     /label="Loop G"
     primer_bind     3875..3901
                     /Sequence="CTAAAGAGCGACGGCTTCGCCAATAGG"
                     /Transferred_From="oBK589"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(3914..3940)
                     /Sequence="GAAGGTTAAAGAGTCATCATGGATCAG"
                     /Hairpin_Tm="None"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="40.7"
                     /created_by="primer3"
                     /label="oBK397"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(3914..3940)
                     /Sequence="GAAGGTTAAAGAGTCATCATGGATCAG"
                     /Transferred_From="oBK397"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     3914..3940
                     /Sequence="CTGATCCATGATGACTCTTTAACCTTC"
                     /Hairpin_Tm="None"
                     /Tm="59.8"
                     /Self_Dimer_Tm="None"
                     /%GC="40.7"
                     /created_by="primer3"
                     /label="oBK428"
     primer_bind     complement(3936..3965)
                     /Sequence="CCTGTGCCTTTTGTATATCCTCTTTGAAGG"
                     /Transferred_From="oBK588"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     4199..4223
                     /Sequence="CTGGGCAGCCAGATCTTAAAGGAGC"
                     /Transferred_From="JS4591"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     4205..4234
                     /created_by="User"
                     /label="Loop H"
     misc_feature    complement(4831..4855)
                     /Sequence="CACAAACTCACTTTCTAGCTTCGGG"
                     /Hairpin_Tm="None"
                     /Tm="62.0"
                     /Self_Dimer_Tm="None"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label="oBK429"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     4831..4855
                     /Sequence="CCCGAAGCTAGAAAGTGAGTTTGTG"
                     /Transferred_From="JS4592"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(4831..4855)
                     /Sequence="CACAAACTCACTTTCTAGCTTCGGG"
                     /Transferred_From="oBK429"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     sig_peptide     5027..5035
                     /created_by="User"
                     /label="Loop I"
     sig_peptide     5282..5290
                     /created_by="User"
                     /label="Loop J"
     primer_bind     5441..5472
                     /Sequence="GAGTTAGAAAATGGCCGAAAACGGATGTTGGC"
                     /Transferred_From="oBK602"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     5453..5472
                     /Sequence="GGCCGAAAACGGATGTTGGC"
                     /Transferred_From="oBK309"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(5476..5496)
                     /Sequence="CCCTTTTGAAGCTCTCCGGCG"
                     /Hairpin_Tm="37.3"
                     /Tm="63.9"
                     /Self_Dimer_Tm="None"
                     /%GC="61.9"
                     /created_by="primer3"
                     /label="oBK398"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5476..5496)
                     /Sequence="CCCTTTTGAAGCTCTCCGGCG"
                     /Transferred_From="oBK398"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     5506..5527
                     /Sequence="CGCACTACCGTCTAAATACGTG"
                     /Transferred_From="JS4593"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     5903..5927
                     /Sequence="CGGATAGATTTGTCACAGCTTGGGG"
                     /Transferred_From="oBK604"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     ORF             5933..6031
                     /created_by="User"
                     /label="NLS-FLAG"
     primer_bind     5934..5959
                     /Sequence="GATCCCCCAAGAAGAAGAGGAAAGTC"
                     /Transferred_From="oBK563"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(5941..5965)
                     /Sequence="GCTCGAGACTTTCCTCTTCTTCTTG"
                     /Hairpin_Tm="None"
                     /Tm="61.4"
                     /Self_Dimer_Tm="8.2"
                     /%GC="48.0"
                     /created_by="primer3"
                     /label="oBK130"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(5964..5987)
                     /Sequence="CACCGTCATGGTCTTTGTAGTCGC"
                     /Transferred_From="oBK591"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     5964..5987
                     /Sequence="GCGACTACAAAGACCATGACGGTG"
                     /Transferred_From="oBK590"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     ORF             6032..>6055
                     /created_by="User"
                     /modified_by="User"
                     /label="Vp64"
     misc_feature    complement(6038..6067)
                     /Sequence="CTGGAACTCCATCCCGCTTCCACCGCCTCC"
                     /Hairpin_Tm="46.5"
                     /Tm="74.6"
                     /Self_Dimer_Tm="0.5"
                     /%GC="66.7"
                     /created_by="primer3"
                     /label="oBK605"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     6044..6077
                     /Sequence="GGTGGAAGCGGGATGGAGTTCCAGTACCTGCCAG"
                     /Hairpin_Tm="51.7"
                     /Tm="74.8"
                     /Self_Dimer_Tm="23.7"
                     /%GC="61.8"
                     /created_by="primer3"
                     /label="oBK606"
     misc_feature    6056..6859
                     /Original_Bases="CGCGCCGACGCGCTGGACGATTTCGATCTCGACATGCTGGG
                     TTCTGATGCCCTCGATGACTTTGACCTGGATATGTTGGGAAGCGACGCATTGGATGAC
                     TTTGATCTGGACATGCTCGGCTCCGATGCTCTGGACGATTTCGATCTCGATATGTTA"
                     /label="CGCGCCGACGCGCTGGACGATTTCGATCTCGACATGCTGGGTTCTGATGC
                     CCTCGATGACTTTGACCTGGATATGTTGGGAAGCGACGCATTGGATGACTTTGATCTG
                     GACATGCTCGGCTCCGATGCTCTGGACGATTTCGATCTCGATATGTTA"
                     /note="Geneious type: Editing History Replacement"
     sig_peptide     6056..6859
                     /created_by="User"
                     /modified_by="User"
                     /label="p65 domain"
     misc_feature    complement(6838..6891)
                     /Sequence="GCAGCCTGCACCTGAGGAGTGCGGCCGCTTTAAGAGCTGATCTGACT
                     CAGCAGG"
                     /created_by="User"
                     /label="oBK607"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6866..6891)
                     /Sequence="GCAGCCTGCACCTGAGGAGTGCGGCC"
                     /Transferred_From="oBK247"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(6870..6898)
                     /Sequence="CTGATAGGCAGCCTGCACCTGAGGAGTGC"
                     /Transferred_From="oBK584"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     misc_feature    complement(6892..6910)
                     /Sequence="AGCCACCACCTTCTGATAG"
                     /Hairpin_Tm="None"
                     /Tm="56.1"
                     /Self_Dimer_Tm="None"
                     /%GC="52.6"
                     /created_by="primer3"
                     /label="SQT368"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(6892..6910)
                     /Sequence="AGCCACCACCTTCTGATAG"
                     /Transferred_From="SQT368"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(6940..6962)
                     /Sequence="CAGAGGGAAAAAGATCTCAGTGG"
                     /Transferred_From="oBK23"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     terminator      7037..7538
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""rb_glob_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY \"
                     /label="rb_glob_PA_terminator"
     primer_bind     complement(7428..7444)
                     /created_by="primer3"
                     /annotation_group="135473983514354300085f78ef4-0452-4fae-a
                     bba-93b35302c171"
                     /%GC="47.1"
                     /Tm="49.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAGGAAACAGCTATGAC"
                     /Transferred_From="M13-R (-26)"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(7441..7464)
                     /created_by="primer3"
                     /annotation_group="1354739835389783000b8ada402-17c3-46ef-9
                     adc-177f91dab407"
                     /%GC="45.8"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGCGGATAACAATTTCACACAGG"
                     /Transferred_From="M13-R (-46)"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     promoter        complement(7580..7598)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""M13_reverse_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /label="M13_reverse_primer"
     misc_feature    complement(7597..7619)
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""M13_pUC_rev_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /label="M13_pUC_rev_primer"
     promoter        complement(7633..7662)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""lac_promoter"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSK \"
                     /label="lac_promoter"
     promoter        7768..7947
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_promoter"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALS \"
                     /label="SV40_promoter"
     rep_origin      7803..7880
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_origin"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKD \"
                     /label="SV40_origin"
     misc_feature    7865..7884
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40pro_F_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQS \"
                     /label="SV40pro_F_primer"
     terminator      7961..8092
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""SV40_PA_terminator"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLST \"
                     /label="SV40_PA_terminator"
     misc_feature    8049..8068
                     /SerialCloner_Color="&hDC143C"
                     /SerialCloner_Show="False"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""EBV_rev_primer"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL \"
                     /label="EBV_rev_primer"
     primer_bind     complement(8056..8075)
                     /created_by="primer3"
                     /annotation_group="13547385713896020002e37130e-0600-4795-b
                     4cf-db36738ae7a4"
                     /%GC="50.0"
                     /Tm="58.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATTACCGCCTTTGAGTGAGC"
                     /modified_by="User"
                     /Transferred_From="VR"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     primer_bind     complement(8192..8211)
                     /Sequence="TTTGTGATGCTCGTCAGGGG"
                     /Transferred_From="SQT757"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     rep_origin      complement(8316..8935)
                     /SerialCloner_Color="&h84A4C0"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""pBR322_origin"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALS \"
                     /label="pBR322_origin"
     primer_bind     8990..9010
                     /Sequence="CCATAGTTGCCTGACTCCCCG"
                     /Transferred_From="oBK46"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     gene            complement(9090..25)
                     /SerialCloner_Color="&hFF4500"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""Ampicillin"" -
                     gene=""Ampicillin"" -
                     translation=""MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     -
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     -
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     - GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDG \"
                     /label="Ampicillin_Ampicillin"
     primer_bind     9830..9851
                     /Sequence="AGGGTTATTGTCTCATGAGCGG"
                     /Transferred_From="SQT379"
                     /Transferred_Similarity="100.00%"
                     /label="Binding Region"
     promoter        complement(9992..95)
                     /SerialCloner_Color="&hF08080"
                     /SerialCloner_Show="True"
                     /SerialCloner_Protect="True"
                     /SerialCloner_Arrow="True"
                     /SerialCloner_Desc="label=""AmpR_promoter"" -
                     translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     -
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     -
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     - DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWM \"
                     /label="AmpR_promoter"
ORIGIN      
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat ggcgccgaaa aaaaaacgca aagtgaacgg
     1801 cggagggtcc ggaggaggcg gtagcggagg cgataaaaag tattctattg gtttagccat
     1861 cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa
     1921 atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct
     1981 cctattcgat agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag
     2041 gtatacacgt cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc
     2101 caaagttgac gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa
     2161 gaaacatgaa cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa
     2221 gtacccaacg atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct
     2281 gaggttaatc tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga
     2341 gggtgatcta aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac
     2401 ctataatcag ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat
     2461 tcttagcgcc cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg
     2521 agagaagaaa aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa
     2581 ttttaagtcg aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta
     2641 cgatgacgat ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt
     2701 ggctgccaaa aaccttagcg atgcaatcct cctatctgac atactgagag ttaatactga
     2761 gattaccaag gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga
     2821 cttgacactt ctcaaggccc tagtccgtca gcaactgcct gagaaatata aggaaatatt
     2881 ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga gtcaagagga
     2941 attctacaag tttatcaaac ccatattaga gaagatggat gggacggaag agttgcttgt
     3001 aaaactcaat cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg gtagcattcc
     3061 acatcaaatc cacttaggcg aattgcatgc tatacttaga aggcaggagg atttttatcc
     3121 gttcctcaaa gacaatcgtg aaaagattga gaaaatccta acctttcgca taccttacta
     3181 tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg atgacaagaa agtccgaaga
     3241 aacgattact ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt
     3301 catcgagagg atgaccaact ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca
     3361 cagtttactt tacgagtatt tcacagtgta caatgaactc acgaaagtta agtatgtcac
     3421 tgagggcatg cgtaaacccg cctttctaag cggagaacag aagaaagcaa tagtagatct
     3481 gttattcaag accaaccgca aagtgacagt taagcaattg aaagaggact actttaagaa
     3541 aattgaatgc ttcgattctg tcgagatctc cggggtagaa gatcgattta atgcgtcact
     3601 tggtacgtat catgacctcc taaagataat taaagataag gacttcctgg ataacgaaga
     3661 gaatgaagat atcttagaag atatagtgtt gactcttacc ctctttgaag atcgggaaat
     3721 gattgaggaa agactaaaaa catacgctca cctgttcgac gataaggtta tgaaacagtt
     3781 aaagaggcgt cgctatacgg gctggggacg attgtcgcgg aaacttatca acgggataag
     3841 agacaagcaa agtggtaaaa ctattctcga ttttctaaag agcgacggct tcgccaatag
     3901 gaactttatg cagctgatcc atgatgactc tttaaccttc aaagaggata tacaaaaggc
     3961 acaggtttcc ggacaagggg actcattgca cgaacatatt gcgaatcttg ctggttcgcc
     4021 agccatcaaa aagggcatac tccagacagt caaagtagtg gatgagctag ttaaggtcat
     4081 gggacgtcac aaaccggaaa acattgtaat cgagatggca cgcgaaaatc aaacgactca
     4141 gaaggggcaa aaaaacagtc gagagcggat gaagagaata gaagagggta ttaaagaact
     4201 gggcagccag atcttaaagg agcatcctgt ggaaaatacc caattgcaga acgagaaact
     4261 ttacctctat tacctacaaa atggaaggga catgtatgtt gatcaggaac tggacataaa
     4321 ccgtttatct gattacgacg tcgatgccat tgtaccccaa tcctttttga aggacgattc
     4381 aatcgacaat aaagtgctta cacgctcgga taagaaccga gggaaaagtg acaatgttcc
     4441 aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa atgcgaaact
     4501 gataacgcaa agaaagttcg ataacttaac taaagctgag aggggtggct tgtctgaact
     4561 tgacaaggcc ggatttatta aacgtcagct cgtggaaacc cgccaaatca caaagcatgt
     4621 tgcacagata ctagattccc gaatgaatac gaaatacgac gagaacgata agctgattcg
     4681 ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg gacttcagaa aggattttca
     4741 attctataaa gttagggaga taaataacta ccaccatgcg cacgacgctt atcttaatgc
     4801 cgtcgtaggg accgcactca ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg
     4861 tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg agataggcaa
     4921 ggctacagcc aaatacttct tttattctaa cattatgaat ttctttaaga cggaaatcac
     4981 tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga
     5041 aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca
     5101 agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct
     5161 tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta
     5221 cggtggcttc gatagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa
     5281 gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg
     5341 ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa
     5401 aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa
     5461 acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa
     5521 atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga
     5581 taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga
     5641 gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt
     5701 aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca
     5761 tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat
     5821 agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc
     5881 catcacggga ttatatgaaa ctcggataga tttgtcacag cttgggggtg acggatcccc
     5941 caagaagaag aggaaagtct cgagcgacta caaagaccat gacggtgatt ataaagatca
     6001 tgacatcgat tacaaggatg acgatgacaa ggctgcagga ggcggtggaa gcgggatgga
     6061 gttccagtac ctgccagata cagacgatcg tcaccggatt gaggagaaac gtaaaaggac
     6121 atatgagacc ttcaagagca tcatgaagaa gagtcctttc agcggaccca ccgacccccg
     6181 gcctccacct cgacgcattg ctgtgccttc ccgcagctca gcttctgtcc ccaagccagc
     6241 accccagccc tatcccttta cgtcatccct gagcaccatc aactatgatg agtttcccac
     6301 catggtgttt ccttctgggc agatcagcca ggcctcggcc ttggccccgg cccctcccca
     6361 agtcctgccc caggctccag cccctgcccc tgctccagcc atggtatcag ctctggccca
     6421 ggccccagcc cctgtcccag tcctagcccc aggccctcct caggctgtgg ccccacctgc
     6481 ccccaagccc acccaggctg gggaaggaac gctgtcagag gccctgctgc agctgcagtt
     6541 tgatgatgaa gacctggggg ccttgcttgg caacagcaca gacccagctg tgttcacaga
     6601 cctggcatcc gtcgataact ccgagtttca gcagctgctg aaccagggca tacctgtggc
     6661 cccccacaca actgagccca tgctgatgga gtaccctgag gctataactc gcctagtgac
     6721 aggggcccag aggccccccg acccagctcc tgctccactg ggggccccgg ggctccccaa
     6781 tggcctcctt tcaggagatg aagacttctc ctccattgcg gacatggact tctcagccct
     6841 gctgagtcag atcagctctt aaagcggccg cactcctcag gtgcaggctg cctatcagaa
     6901 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     6961 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     7021 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     7081 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     7141 atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga
     7201 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     7261 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     7321 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     7381 tcttatgaag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
     7441 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     7501 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     7561 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
     7621 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7681 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7741 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     7801 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7861 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7921 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     7981 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     8041 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     8101 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     8161 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     8221 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     8281 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     8341 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     8401 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     8461 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     8521 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     8581 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     8641 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     8701 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     8761 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     8821 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     8881 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     8941 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     9001 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     9061 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     9121 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     9181 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     9241 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     9301 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     9361 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     9421 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     9481 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     9541 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     9601 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     9661 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     9721 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     9781 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     9841 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     9901 acatttcccc gaaaagtgcc acctg
//
