LOCUS       12790_to_12057_o       13101 bp    DNA     circular SYN 07-Nov-2016
DEFINITION  .
FEATURES             Location/Qualifiers
     source          <110..>402
     source          382..>402
                     /note=" [Split]"
     source          <406..>469
     source          <406..>469
     source          <470..489
     source          <470..489
                     /note=" [Split]"
     source          <500..>1477
                     /mol_type="genomic DNA"
                     /organism="Macacine herpesvirus 3"
                     /country="USA"
                     /culture_collection="ATCC:VR-677"
                     /db_xref="taxon:47929"
                     /host="Macaca mulatta"
                     /isolation_source="urine from a rhesus macaque"
                     /note=" [Split]"
                     /strain="68-1"
     source          <1505..>1507
     source          <1511..>1514
                     /mol_type="other DNA"
                     /organism="pEF1/V5 His C"
                     /note=" [Split]"
     source          1516..>1534
                     /mol_type="other DNA"
                     /organism="pInducer10-mir-RUP-PheS"
                     /note=" [Split]"
     source          <1535..>1542
                     /mol_type="other DNA"
                     /organism="pInducer10-mir-RUP-PheS"
                     /note=" [Split]"
     promoter        complement(5..23)
                     /label="T7_promoter"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     frag            89..>402
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /note="21 to 422 of TRE3G PCR PROD2 [Split]"
                     /right_cutter="AgeI (422)"
                     /right_end="5CCGG"
     frag            <89..93
                     /date="31-Dec-2015"
                     /note="1 to 25 of FWD2 TRE3G [Split]"
     frag            <94..109
                     /date="10-Dec-2015"
                     /note="1 to 291 of Untitled [Split]"
     frag            94..>402
                     /date="31-Dec-2015"
                     /note="1081 to 1483 of VB plasmid Seq-corrected [Split]"
     frag            <110..>402
                     /note=" [Split]"
     promoter        <114..488
                     /note="Tet-responsive Element 3G [Split]"
     misc_binding    118..137
                     /note="tet-operator"
     misc_binding    154..173
                     /note="tet-operator"
     misc_binding    190..209
                     /note="tet-operator"
     misc_binding    226..245
                     /note="tet-operator"
     misc_binding    262..281
                     /note="tet-operator"
     misc_binding    298..317
                     /note="tet-operator"
     misc_binding    334..353
                     /note="tet-operator"
     misc_feature    402..416
                     /note="crs (mutated)"
     frag            <406..>469
     frag            <406..>469
     frag            <406..>469
     frag            <406..>469
     frag            <470..489
                     /note=" [Split]"
     frag            <470..489
                     /note=" [Split]"
     frag            <470..489
                     /date="18-Aug-2016"
                     /left_cutter="AgeI (489)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="490 to 1479 of pOUP UL148HA plus 1202 to 489 of
                     pOUPc-RFP [Split]"
                     /right_cutter="AgeI (489)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     misc_feature    470..489
                     /note="gibs"
     frag            <470..489
                     /note=" [Split]"
     frag            <470..489
                     /date="18-Aug-2016"
                     /left_cutter="MluI (1201)"
                     /left_end="5CGCG"
                     /note="1202 to 489 of pOUPc-RFP [Split]"
                     /right_cutter="AgeI (489)"
                     /right_end="5CCGG"
     frag            <470..489
                     /note=" [Split]"
     frag            470..>489
                     /date="24-Oct-2016"
                     /note="1 to 32 of FWD UL16HA gibs [Split]"
     frag            470..>489
                     /date="24-Oct-2016"
                     /note="13051 to 11 of pOUPc UL148HA [Split]"
     frag            join(470..>489,<1515..1534)
                     /date="24-Oct-2016"
                     /note="1 to 778 of PCR prod theoretical [Split]"
     frag            490..496
                     /date="19-Nov-2021"
                     /note="61 to 67 of poUPc-Rh159-HA-1-CMV"
     frag            join(<497..>1508,<1511..>1514)
                     /date="07-Nov-2016"
                     /left_cutter="EcoRI (952)"
                     /left_end="5AATT"
                     /note="953 to 1999 of pcDNA3.1_+_Rh159_HA [Split]"
                     /right_cutter="XhoI (1999)"
                     /right_end="5TCGA"
     frag            <497..499
                     /date="11-Oct-2016"
                     /note="374 to 401 of RH159-T7_G07.ab1"
     frag            join(<497..>1508,<1511..>1514)
                     /date="18-Oct-2016"
                     /note=" [Split]"
     frag            join(<497..>1508,<1511..>1514,1523..>13101,1..>489)
                     /date="07-Nov-2016"
                     /left_cutter="XhoI (12789)"
                     /left_end="5TCGA"
                     /left_join="keep end"
                     /note="12790 to 12057 of pOUPcex UL16HA plus 953 to 1999 of
                     pcDNA3.1_+_Rh159_HA"
                     /right_cutter="XhoI (1999)"
                     /right_end="5TCGA"
                     /right_join="keep end"
     frag            500..>1477
                     /date="11-Oct-2016"
                     /note="164371 to 165351 of RhCMV CG [Split]"
     gene            500..>1477
                     /db_xref="GeneID:2952701"
                     /locus_tag="grh159"
                     /note=" [Split]"
     CDS             500..>1477
                     /codon_start=1
                     /db_xref="GI:51556614"
                     /db_xref="GeneID:2952701"
                     /locus_tag="grh159"
                     /note="similar to UL148 [Split]"
                     /product="rh159"
                     /protein_id="YP_068248.1"
                     /translation="MAYNSFLLSCLTIALLLHGLPTSTSAKDMAGYVHVHMLNAPEDAA
                     STSAPVIQLANGFITCRKFTELGWALCTSWDDMECFPFWRRTFSETKNITVLSDDPYYA
                     GVVLTVRTLLGDSLELTSYLGVRPSIANVGYYVMYLSYAKALPAKFTHNDVDYVRFAIY
                     LHTEIDAQQTPDNNFVFTFATPGTFETPDLMSSFVIGTDDGQNSTLNFTTVGGEKHRYN
                     NVELSSEMKGNCVYHSAIIRNVASSQSWVSFDFSLYWRRTKIAYSYVQYYFPKPLHSPE
                     PIGPAWQRIYHYFSLLTQLRYGTVAITVVLIFLIGALLKRSREAH"
     primer_bind     528..548
                     /note="Fw"
     primer_bind     529..550
                     /note="Fw_2"
     primer_bind     complement(1449..1468)
                     /note="Rv"
     frag            <1478..>1504
                     /note="219793 to 224176 of UL135_HAPPyFLAG_Kan_INT [Split]"
     frag            <1478..>1504
                     /note="1 to 60 of HAPPy_FLAGstop [Split]"
     frag            <1478..>1504
                     /note="1 to 1108 of HAPPy_FLAG_Kan [Split]"
     primer_bind     1478..1497
                     /note="HA forward primer"
     CDS             1478..1504
                     /note="HA eptiope"
     frag            1478..1504
                     /date="11-Oct-2016"
                     /left_end="0"
                     /note="1 to 27 of HA tag nt"
                     /right_end="0"
     CDS             <1505..1507
     gene            <1505..1507
     frag            <1505..1507
     frag            1511..>1514
                     /date="11-Oct-2016"
                     /left_end="0"
                     /note="63 to 82 of Untitled"
                     /right_end="0"
     frag            complement(<1511..>1514)
                     /date="11-Oct-2016"
                     /note=" [Split]"
     misc_recomb     <1511..>1514
                     /note=" [Split]"
     frag            join(<1515..>13101,<1..>467)
                     /date="07-Nov-2016"
                     /left_cutter="XhoI (12789)"
                     /left_end="5TCGA"
                     /note="12790 to 12057 of pOUPcex UL16HA"
                     /right_cutter="EcoRI (12057)"
                     /right_end="5AATT"
     frag            <1515..>1534
                     /date="27-Jun-2016"
                     /note="1 to 60 of ssDNA oligo [Split]"
     misc_feature    complement(1515..1534)
                     /note="gibs"
     frag            <1515..1534
                     /date="24-Oct-2016"
                     /note="990 to 1014 of pOUPc UL148HA"
     frag            <1515..>1534
                     /date="18-Aug-2016"
                     /left_cutter="AgeI (489)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note=" [Split]"
                     /right_cutter="AgeI (489)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     frag            <1515..>1534
                     /date="18-Aug-2016"
                     /left_cutter="MluI (1201)"
                     /left_end="5CGCG"
                     /note="1202 to 489 of pOUPc-RFP [Split]"
                     /right_cutter="AgeI (489)"
                     /right_end="5CCGG"
     frag            complement(<1515..1534)
                     /date="24-Oct-2016"
                     /note="1 to 26 of REV UL16HA gibs [Split]"
     frag            1516..>1534
                     /date="12-Jan-2016"
                     /left_cutter="MluI (4795)"
                     /left_end="5CGCG"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS [Split]"
                     /right_cutter="AgeI (2375)"
                     /right_end="5CCGG"
     frag            1516..>1534
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS plus 1600 to
                     866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     frag            1516..>1534
                     /date="19-Feb-2016"
                     /left_cutter="NotI (1599)"
                     /left_end="5GGCC"
                     /note="1600 to 866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
     frag            1516..>1534
                     /date="12-Jan-2016"
                     /left_cutter="AgeI (12147)"
                     /left_end="5CCGG"
                     /note="12148 to 11678 of pINDUCER10 dTomato-mirE (STEP ONE)
                     [Split]"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
     frag            1516..>1534
                     /date="12-Jan-2016"
                     /left_cutter="MluI (4795)"
                     /left_end="5CGCG"
                     /left_join="keep end"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS plus 83 to
                     1222 of pSP72 dTomato_mirE [Split]"
                     /right_cutter="MluI (1222)"
                     /right_end="5CGCG"
                     /right_join="keep end"
     frag            1516..>1534
                     /date="20-Jun-2016"
                     /note="1612 to 1641 of pOUP-RFP [Split]"
     frag            1516..>1534
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /left_join="keep end"
                     /note="21 to 422 of TRE3G PCR PROD2 plus 12148 to 11678 of
                     pINDUCER10 dTomato-mirE (STEP ONE) [Split]"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
                     /right_join="keep end"
     promoter        1519..>1534
                     /label="hUbC_promoter"
                     /note="/translation=""MHHPAAQHFQPFTVFTHDVHFCRRFGKREVGRTEANL
                     QIFLKEV
                     TQEVVQRAFQVGKADVGINNQTFHLMEHRRVSLVVVVTINTARRDDTNRWLLGFHGAD
                     LYARGLGTQQAGSVKPESVVVSARRVVTRNVQSIEVMIIVFDFRPGCHGKAKLTEETF
                     DTVDGTGNRMQTAVFNATTWQRDINRFRRQTRIQCSAFQFRFTRIQRLLNLLFRFVDN
                     RTSCRTLFWRQLTQGRHLKG*"" [Split]"
     frag            <1535..>1542
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /left_join="keep end"
                     /note="21 to 422 of TRE3G PCR PROD2 plus 12148 to 11678 of
                     pINDUCER10 dTomato-mirE (STEP ONE) [Split]"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
                     /right_join="keep end"
     frag            <1535..>1542
                     /date="19-Feb-2016"
                     /left_cutter="NotI (1599)"
                     /left_end="5GGCC"
                     /note="1600 to 866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
     misc_feature    <1535..>1542
                     /note="oligo gibs"
     frag            <1535..>1542
                     /date="12-Jan-2016"
                     /left_cutter="MluI (4795)"
                     /left_end="5CGCG"
                     /left_join="keep end"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS plus 83 to
                     1222 of pSP72 dTomato_mirE [Split]"
                     /right_cutter="MluI (1222)"
                     /right_end="5CGCG"
                     /right_join="keep end"
     frag            <1535..>1542
                     /date="12-Jan-2016"
                     /left_cutter="AgeI (12147)"
                     /left_end="5CCGG"
                     /note="12148 to 11678 of pINDUCER10 dTomato-mirE (STEP ONE)
                     [Split]"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
     frag            join(<1535..>13101,<1..>469)
                     /date="18-Aug-2016"
                     /left_cutter="MluI (1201)"
                     /left_end="5CGCG"
                     /note="1202 to 489 of pOUPc-RFP"
                     /right_cutter="AgeI (489)"
                     /right_end="5CCGG"
     frag            <1535..>1542
                     /date="19-Feb-2016"
                     /left_cutter="AgeI (2375)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="2376 to 3086 of pINDUCER10-mir-RUP-PheS plus 1600 to
                     866 of pINDv2 [Split]"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     frag            <1535..>1542
                     /date="12-Jan-2016"
                     /left_cutter="MluI (4795)"
                     /left_end="5CGCG"
                     /note="4796 to 2375 of pINDUCER10-mir-RUP-PheS [Split]"
                     /right_cutter="AgeI (2375)"
                     /right_end="5CCGG"
     frag            <1535..1542
                     /date="27-Jun-2016"
                     /note="1 to 60 of ssDNA oligo [Split]"
     frag            <1535..>1542
                     /date="20-Jun-2016"
                     /note="1612 to 1641 of pOUP-RFP"
     promoter        <1535..2735
                     /label="hUbC_promoter"
                     /note="/translation=""MHHPAAQHFQPFTVFTHDVHFCRRFGKREVGRTEANL
                     QIFLKEV
                     TQEVVQRAFQVGKADVGINNQTFHLMEHRRVSLVVVVTINTARRDDTNRWLLGFHGAD
                     LYARGLGTQQAGSVKPESVVVSARRVVTRNVQSIEVMIIVFDFRPGCHGKAKLTEETF
                     DTVDGTGNRMQTAVFNATTWQRDINRFRRQTRIQCSAFQFRFTRIQRLLNLLFRFVDN
                     RTSCRTLFWRQLTQGRHLKG*"" [Split]"
     frag            join(<1535..>13101,<1..>469)
                     /date="18-Aug-2016"
                     /left_cutter="AgeI (489)"
                     /left_end="5CCGG"
                     /left_join="keep end"
                     /note="490 to 1479 of pOUP UL148HA plus 1202 to 489 of
                     pOUPc-RFP"
                     /right_cutter="AgeI (489)"
                     /right_end="5CCGG"
                     /right_join="keep end"
     frag            join(<1543..>13101,<1..>24)
                     /date="12-Jan-2016"
                     /left_cutter="NheI (20)"
                     /left_end="5CTAG"
                     /left_join="keep end"
                     /note="21 to 422 of TRE3G PCR PROD2 plus 12148 to 11678 of
                     pINDUCER10 dTomato-mirE (STEP ONE)"
                     /right_cutter="NheI (11678)"
                     /right_end="5CTAG"
                     /right_join="keep end"
     frag            join(<1543..13101,<1..24)
                     /date="19-Feb-2016"
                     /left_cutter="NotI (1599)"
                     /left_end="5GGCC"
                     /note="1600 to 866 of pINDv2"
                     /right_cutter="AgeI (866)"
                     /right_end="5CCGG"
     frag            join(<1546..>13101,<1..>402)
     misc_feature    2717..2736
                     /note="gibs?"
     misc_feature    complement(2742..2775)
                     /label="loxP"
                     /note="/translation=""MHHPAAQHFQPFTVFTHDVHFCRRFGKREVGRTEANL
                     QIFLKEV
                     TQEVVQRAFQVGKADVGINNQTFHLMEHRRVSLVVVVTINTARRDDTNRWLLGFHGAD
                     LYARGLGTQQAGSVKPESVVVSARRVVTRNVQSIEVMIIVFDFRPGCHGKAKLTEETF
                     DTVDGTGNRMQTAVFNATTWQRDINRFRRQTRIQCSAFQFRFTRIQRLLNLLFRFVDN
                     RTSCRTLFWRQLTQGRHLKG*"""
     CDS             2755..3528
                     /label="ORF frame 3"
                     /translation="MYAIRSYATMSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGV
                     EQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRD
                     GAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVA
                     KEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALD
                     DFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"
     misc_feature    3529..3562
                     /label="loxP"
                     /note="/translation=""MYAIRSYATMSRLDKSKVINGALELLNGVGIEGLTTR
                     KLAQKLG
                     VEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSH
                     RDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEH
                     QVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPT
                     DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"""
     misc_feature    3577..4140
                     /label="IRES"
                     /note="/translation=""MYAIRSYATMSRLDKSKVINGALELLNGVGIEGLTTR
                     KLAQKLG
                     VEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSH
                     RDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEH
                     QVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPT
                     DALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG*"""
     CDS             4153..4755
                     /label="ORF frame 3"
                     /translation="MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
     gene            4168..4755
                     /gene="puro (variant)"
                     /label="puro (variant)"
                     /note="/translation=""MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHT
                     VDPDRHI
                     ERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGS
                     RLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF
                     LETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"""
     misc_feature    4756..4778
                     /note="gibs"
     misc_feature    4779..5354
                     /label="WPRE"
                     /note="/translation=""MATEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHT
                     VDPDRHI
                     ERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGSRMAELSGS
                     RLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAF
                     LETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"""
     CDS             4867..5394
                     /label="ORF frame 3"
                     /translation="MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFA
                     DATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLT
                     SFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWA
                     ASPPGINSAVET*"
     misc_feature    5572..5624
                     /label="delta_U3"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    5625..5805
                     /label="HIV-1_5_LTR"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    5625..5805
                     /label="truncHIV-1_3_LTR"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    complement(5906..5923)
                     /label="BGH_rev_primer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     terminator      5909..6136
                     /label="bGH_PA_terminator"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     rep_origin      6199..6505
                     /label="f1_origin"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    complement(6619..6639)
                     /label="pBABE_3_primer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    complement(6625..6840)
                     /label="SV40_enhancer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     promoter        6637..6905
                     /label="SV40_promoter"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     rep_origin      6804..6881
                     /label="SV40_origin"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     misc_feature    6866..6885
                     /label="SV40pro_F_primer"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     gene            7032..7643
                     /gene="hygroB (420 - 1031)"
                     /label="hygroB (420 - 1031)"
                     /note="/translation=""MPLYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQR
                     GVVCTVF
                     ADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGK
                     LTSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRIS
                     LWAASPPGINSAVET*"""
     CDS             7116..7646
                     /label="ORF frame 2"
                     /translation="MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGR
                     ITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRI
                     GLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADS
                     GNRRPSTRPRAKE*"
     terminator      7779..7898
                     /label="SV40_PA_terminator"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     misc_feature    7867..7886
                     /label="EBV_rev_primer"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     promoter        complement(7942..7960)
                     /label="M13_reverse_primer"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     misc_feature    complement(7959..7981)
                     /label="M13_pUC_rev_primer"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     promoter        complement(7995..8024)
                     /label="lac_promoter"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     gene            complement(9107..9967)
                     /gene="Ampicillin"
                     /label="Ampicillin"
                     /note="/translation=""MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSN
                     NVLTDNG
                     RITAVIDWSEAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYML
                     RIGLDQLYQSLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVL
                     ADSGNRRPSTRPRAKE*"""
     CDS             complement(9107..9967)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW*"
     promoter        complement(10009..10037)
                     /label="AmpR_promoter"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     terminator      10123..10254
                     /label="SV40_PA_terminator"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     misc_feature    10211..10230
                     /label="EBV_rev_primer"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     misc_feature    10875..11055
                     /label="truncHIV-1_3_LTR"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     misc_feature    10875..11055
                     /label="HIV-1_5_LTR"
                     /note="/translation=""MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQ
                     LGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRMDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"""
     CDS             complement(11492..11953)
                     /label="ORF frame 2"
                     /translation="MPQTVSCNRCCCASIALSKLFCCCTIPDNNCLACTVSVIDAAPIV
                     LPAAPKNPRNKAPIPTALFSLCTTLLFALVGATPNGSIFTTLYLYNSLLQLSLISPPPG
                     LKISGRPLAVRWSYFCFALPLSCLKLPWCLLSLSFDAHNRGLLLYYIMI*"
     CDS             11658..12545
                     /label="ORF frame 3"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGALF
                     LGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQLQA
                     RILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDREINN
                     YTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGLVGLR
                     IVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVNGSALR
                     APILQTNGSIHPQF*"
     misc_feature    11780..12013
                     /label="RRE"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     misc_feature    12544..12559
                     /label="cPPT"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     misc_feature    complement(12722..13096)
                     /label="sh_ble"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
     gene            complement(12725..13096)
                     /gene="bleo"
                     /label="bleo"
                     /note="/translation=""MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKR
                     AVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSALRAPILQTNGSIHPQF*"""
BASE COUNT  3139 A  3368 C  3449 G  3145 T  0 OTHER
ORIGIN
        1 CCCTCCTATA GTGAGTCGTA TTATACTATG CCGATATACT ATGCCGATGA TTAATTGTCA
       61 ACACGTGCTG CAGGTCCGAG GTTCTAGGCT AGCGAGACTA GCGACGAGTT GGCTTTACTC
      121 CCTATCAGTG ATAGAGAACG TATGAAGAGT TTACTCCCTA TCAGTGATAG AGAACGTATG
      181 CAGACTTTAC TCCCTATCAG TGATAGAGAA CGTATAAGGA GTTTACTCCC TATCAGTGAT
      241 AGAGAACGTA TGACCAGTTT ACTCCCTATC AGTGATAGAG AACGTATCTA CAGTTTACTC
      301 CCTATCAGTG ATAGAGAACG TATATCCAGT TTACTCCCTA TCAGTGATAG AGAACGTATA
      361 AGCTTTAGGC GTGTACGGTG GGCGCCTATA AAAGCAGAGC TCAGGTAGTG AACCGTCAGA
      421 TCGCCTGGAG CAATTCCACA ACACTTTTGT CTTATACCAA CTTTCCGTAC CACTTCCTAC
      481 CCTCGTAAAC GGATCCACCA TGGCCTACAA CAGCTTCCTT CTCAGCTGCC TTACCATCGC
      541 ACTTTTACTG CACGGTCTTC CAACATCAAC TAGCGCTAAA GACATGGCAG GCTACGTACA
      601 CGTTCATATG CTTAACGCAC CTGAAGACGC CGCTTCTACG TCTGCGCCAG TTATCCAACT
      661 AGCCAACGGC TTTATTACCT GCAGAAAATT TACAGAGCTC GGTTGGGCAC TGTGTACTAG
      721 CTGGGATGAT ATGGAGTGTT TTCCGTTTTG GCGAAGGACT TTCTCGGAAA CTAAAAATAT
      781 CACTGTGCTT TCAGACGATC CATACTATGC CGGCGTTGTG TTGACAGTAC GGACGCTTCT
      841 TGGTGACTCC CTAGAGCTTA CATCTTATCT CGGGGTCAGG CCTTCGATTG CCAATGTGGG
      901 CTACTATGTA ATGTACCTGT CATATGCTAA GGCGCTCCCG GCAAAATTTA CCCACAACGA
      961 TGTGGACTAT GTGAGGTTTG CCATATACCT TCACACCGAA ATTGATGCGC AGCAAACTCC
     1021 TGACAACAAC TTCGTCTTTA CGTTCGCCAC ACCCGGGACA TTTGAAACCC CAGACCTCAT
     1081 GAGTAGTTTT GTGATTGGAA CCGATGATGG ACAAAATAGC ACCTTGAATT TCACCACTGT
     1141 TGGCGGAGAA AAACACCGCT ACAACAACGT GGAGCTCAGC TCAGAAATGA AGGGCAATTG
     1201 TGTGTATCAC TCGGCTATTA TCCGGAACGT CGCCAGCTCG CAATCTTGGG TGTCGTTTGA
     1261 CTTCTCCCTA TATTGGCGAC GTACCAAAAT TGCATATAGT TACGTGCAGT ATTATTTCCC
     1321 GAAACCACTC CATTCACCTG AACCCATAGG TCCTGCCTGG CAGAGAATCT ACCATTACTT
     1381 TAGCCTCCTG ACGCAGCTTC GCTACGGAAC AGTAGCGATC ACCGTGGTGC TCATTTTCCT
     1441 TATAGGTGCA CTTCTAAAAC GCAGTCGTGA AGCTCATTAC CCATACGACG TCCCAGACTA
     1501 CGCCTAgtga ATtCTTAAGA TCTGGCCTCC GCGCCGGGTT TTGGCGCCTC CCGCGGGCGC
     1561 CCCCCTCCTC ACGGCGAGCG CTGCCACGTC AGACGAAGGG CGCAGCGAGC GTCCTGATCC
     1621 TTCCGCCCGG ACGCTCAGGA CAGCGGCCCG CTGCTCATAA GACTCGGCCT TAGAACCCCA
     1681 GTATCAGCAG AAGGACATTT TAGGACGGGA CTTGGGTGAC TCTAGGGCAC TGGTTTTCTT
     1741 TCCAGAGAGC GGAACAGGCG AGGAAAAGTA GTCCCTTCTC GGCGATTCTG CGGAGGGATC
     1801 TCCGTGGGGC GGTGAACGCC GATGATTATA TAAGGACGCG CCGGGTGTGG CACAGCTAGT
     1861 TCCGTCGCAG CCGGGATTTG GGTCGCGGTT CTTGTTTGTG GATCGCTGTG ATCGTCACTT
     1921 GGTGAGTAGC GGGCTGCTGG GCTGGCCGGG GCTTTCGTGG CCGCCGGGCC GCTCGGTGGG
     1981 ACGGAAGCGT GTGGAGAGAC CGCCAAGGGC TGTAGTCTGG GTCCGCGAGC AAGGTTGCCC
     2041 TGAACTGGGG GTTGGGGGGA GCGCAGCAAA ATGGCGGCTG TTCCCGAGTC TTGAATGGAA
     2101 GACGCTTGTG AGGCGGGCTG TGAGGTCGTT GAAACAAGGT GGGGGGCATG GTGGGCGGCA
     2161 AGAACCCAAG GTCTTGAGGC CTTCGCTAAT GCGGGAAAGC TCTTATTCGG GTGAGATGGG
     2221 CTGGGGCACC ATCTGGGGAC CCTGACGTGA AGTTTGTCAC TGACTGGAGA ACTCGGTTTG
     2281 TCGTCTGTTG CGGGGGCGGC AGTTATGGCG GTGCCGTTGG GCAGTGCACC CGTACCTTTG
     2341 GGAGCGCGCG CCCTCGTCGT GTCGTGACGT CACCCGTTCT GTTGGCTTAT AATGCAGGGT
     2401 GGGGCCACCT GCCGGTAGGT GTGCGGTAGG CTTTTCTCCG TCGCAGGACG CAGGGTTCGG
     2461 GCCTAGGGTA GGCTCTCCTG AATCGACAGG CGCCGGACCT CTGGTGAGGG GAGGGATAAG
     2521 TGAGGCGTCA GTTTCTTTGG TCGGTTTTAT GTACCTATCT TCTTAAGTAG CTGAAGCTCC
     2581 GGTTTTGAAC TATGCGCTCG GGGTTGGCGA GTGTGTTTTG TGAAGTTTTT TAGGCACCTT
     2641 TTGAAATGTA ATCATTTGGG TCAATATGTA ATTTTCAGTG TTAGACTAGT AAATTGTCCG
     2701 CTAAATTCTG GCCGTTTTTG GCTTTTTTGT TAGACGGATC CATAACTTCG TATAATGTAT
     2761 GCTATACGAA GTTATGCCAC CATGTCTAGA CTGGACAAGA GCAAAGTCAT AAACGGAGCT
     2821 CTGGAATTAC TCAATGGTGT CGGTATCGAA GGCCTGACGA CAAGGAAACT CGCTCAAAAG
     2881 CTGGGAGTTG AGCAGCCTAC CCTGTACTGG CACGTGAAGA ACAAGCGGGC CCTGCTCGAT
     2941 GCCCTGCCAA TCGAGATGCT GGACAGGCAT CATACCCACT TCTGCCCCCT GGAAGGCGAG
     3001 TCATGGCAAG ACTTTCTGCG GAACAACGCC AAGTCATACC GCTGTGCTCT CCTCTCACAT
     3061 CGCGACGGGG CTAAAGTGCA TCTCGGCACC CGCCCAACAG AGAAACAGTA CGAAACCCTG
     3121 GAAAATCAGC TCGCGTTCCT GTGTCAGCAA GGCTTCTCCC TGGAGAACGC ACTGTACGCT
     3181 CTGTCCGCCG TGGGCCACTT TACACTGGGC TGCGTATTGG AGGAACAGGA GCATCAAGTA
     3241 GCAAAAGAGG AAAGAGAGAC ACCTACCACC GATTCTATGC CCCCACTTCT GAGACAAGCA
     3301 ATTGAGCTGT TCGACCGGCA GGGAGCCGAA CCTGCCTTCC TTTTCGGCCT GGAACTAATC
     3361 ATATGTGGCC TGGAGAAACA GCTAAAGTGC GAAAGCGGCG GGCCGACCGA CGCCCTTGAC
     3421 GATTTTGACT TAGACATGCT CCCAGCCGAT GCCCTTGACG ACTTTGACCT TGATATGCTG
     3481 CCTGCTGACG CTCTTGACGA TTTTGACCTT GACATGCTCC CCGGGTAAAT AACTTCGTAT
     3541 AGCATACATT ATACGAAGTT ATGGCGCGCC AATTCCGCCC CTCTCCCTCC CCCCCCCCTA
     3601 ACGTTACTGG CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT
     3661 CCACCATATT GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA
     3721 CGAGCATTCC TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTGAATGTCG
     3781 TGAAGGAAGC AGTTCCTCTG GAAGCTTCTT GAAGACAAAC AACGTCTGTA GCGACCCTTT
     3841 GCAGGCAGCG GAACCCCCCA CCTGGCGACA GGTGCCTCTG CGGCCAAAAG CCACGTGTAT
     3901 AAGATACACC TGCAAAGGCG GCACAACCCC AGTGCCACGT TGTGAGTTGG ATAGTTGTGG
     3961 AAAGAGTCAA ATGGCTCTCC TCAAGCGTAT TCAACAAGGG GCTGAAGGAT GCCCAGAAGG
     4021 TACCCCATTG TATGGGATCT GATCTGGGGC CTCGGTGCAC ATGCTTTACA TGTGTTTAGT
     4081 CGAGGTTAAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA CGTGGTTTTC CTTTGAAAAA
     4141 CACGATAATA CCATGGCCAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC CCGCGACGAC
     4201 GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC CACGCGCCAC
     4261 ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT CTTCCTCACG
     4321 CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC GGTGGCGGTC
     4381 TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG CTCGCGCATG
     4441 GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT CCTGGCGCCG
     4501 CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC CGACCACCAG
     4561 GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA GCGCGCTGGG
     4621 GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA GCGGCTCGGC
     4681 TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG CATGACCCGC
     4741 AAGCCCGGTG CCTGATTAAT TAAGCGCGTC TGGAACAATC AACCTCTGGA TTACAAAATT
     4801 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT
     4861 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG
     4921 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC
     4981 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT
     5041 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC
     5101 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG
     5161 TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG
     5221 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC
     5281 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG
     5341 ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA ATTAATTCTG CAGTCGAGAC CTAGAAAAAC
     5401 ATGGAGCAAT CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG
     5461 CACAAGAGGA GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA
     5521 CTTACAAGGC AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGAGGGGA CTGGAAGGGC
     5581 TAATTCACTC CCAACGAAGA CAAGATCTGC TTTTTGCTTG TACTGGGTCT CTCTGGTTAG
     5641 ACCAGATCTG AGCCTGGGAG CTCTCTGGCT AACTAGGGAA CCCACTGCTT AAGCCTCAAT
     5701 AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT GTGCCCGTCT GTTGTGTGAC TCTGGTAACT
     5761 AGAGATCCCT CAGACCCTTT TAGTCAGTGT GGAAAATCTC TAGCAGTAGT AGTTCATGTC
     5821 ATCTTATTAT TCAGTATTTA TAACTTGCAA AGAAATGAAT ATCAGAGAGT GAGAGGCCTT
     5881 GACATTGTTT AAACCCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA GCCATCTGTT
     5941 GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC TGTCCTTTCC
     6001 TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT TCTGGGGGGT
     6061 GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA TGCTGGGGAT
     6121 GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCTAG GGGGTATCCC
     6181 CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC
     6241 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC
     6301 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT
     6361 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG
     6421 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT
     6481 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA
     6541 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT
     6601 AACGCGAATT AATTCTGTGG AATGTGTGTC AGTTAGGGTG TGGAAAGTCC CCAGGCTCCC
     6661 CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCAGG TGTGGAAAGT
     6721 CCCCAGGCTC CCCAGCAGGC AGAAGTATGC AAAGCATGCA TCTCAATTAG TCAGCAACCA
     6781 TAGTCCCGCC CCTAACTCCG CCCATCCCGC CCCTAACTCC GCCCAGTTCC GCCCATTCTC
     6841 CGCCCCATGG CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCTGCCTCTG
     6901 AGCTATTCCA GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC
     6961 CGGGAGCTTG TATATCCATT TTCGGATCTG ATCAGCACGT GATGAAAAAG CCTGAACTCA
     7021 CCGCGACGTG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC ATATGCGCGA
     7081 TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC AGTGCGTCCG
     7141 TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA GTCCGGCACC
     7201 TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC ATAACAGCGG
     7261 TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC AACATCTTCT
     7321 TCTGGAGGCC GTGGTTGGCT TGTATGGAGC AGCAGACGCG CTACTTCGAG CGGAGGCATC
     7381 CGGAGCTTGC AGGATCGCCG CGGCTCCGGG CGTATATGCT CCGCATTGGT CTTGACCAAC
     7441 TCTATCAGAG CTTGGTTGAC GGCAATTTCG ATGATGCAGC TTGGGCGCAG GGTCGATGCG
     7501 ACGCAATCGT CCGATCCGGA GCCGGGACTG TCGGGCGTAC ACAAATCGCC CGCAGAAGCG
     7561 CGGCCGTCTG GACCGATGGC TGTGTAGAAG TACTCGCCGA TAGTGGAAAC CGACGCCCCA
     7621 GCACTCGTCC GAGGGCAAAG GAATAGCACG TGCTACGAGA TTTCGATTCC ACCGCCGCCT
     7681 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC
     7741 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG
     7801 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT
     7861 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT
     7921 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC
     7981 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT
     8041 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC
     8101 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG
     8161 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG
     8221 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG
     8281 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC
     8341 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC
     8401 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC
     8461 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT
     8521 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG
     8581 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT
     8641 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG
     8701 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT
     8761 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC
     8821 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA
     8881 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG
     8941 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA
     9001 TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA
     9061 GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA
     9121 TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC
     9181 CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA
     9241 TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA
     9301 GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT
     9361 GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG
     9421 CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC
     9481 AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG
     9541 GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG
     9601 CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT
     9661 ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT
     9721 CCATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC
     9781 GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC
     9841 CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG
     9901 CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA
     9961 TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA
    10021 GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC
    10081 CCCGAAAAGT GCCACCTGAC GTCGACGGAT CGGGAGATCA ACTTGTTTAT TGCAGCTTAT
    10141 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG
    10201 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GATCAACTGG
    10261 ATAACTCAAG CTAACCAAAA TCATCCCAAA CTTCCCACCC CATACCCTAT TACCACTGCC
    10321 AATTACCTGT GGTTTCATTT ACTCTAAACC TGTGATTCCT CTGAATTATT TTCATTTTAA
    10381 AGAAATTGTA TTTGTTAAAT ATGTACTACA AACTTAGTAG TTGGAAGGGC TAATTCACTC
    10441 CCAAAGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA
    10501 TTAGCAGAAC TACACACCAG GGCCAGGGGT CAGATATCCA CTGACCTTTG GATGGTGCTA
    10561 CAAGCTAGTA CCAGTTGAGC CAGATAAGGT AGAAGAGGCC AATAAAGGAG AGAACACCAG
    10621 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TGTTAGAGTG
    10681 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CGTGGCCCGA GAGCTGCATC CGGAGTACTT
    10741 CAAGAACTGC TGATATCGAG CTTGCTACAA GGGACTTTCC GCTGGGGACT TTCCAGGGAG
    10801 GCGTGGCCTG GGCGGGACTG GGGAGTGGCG AGCCCTCAGA TCCTGCATAT AAGCAGCTGC
    10861 TTTTTGCCTG TACTGGGTCT CTCTGGTTAG ACCAGATCTG AGCCTGGGAG CTCTCTGGCT
    10921 AACTAGGGAA CCCACTGCTT AAGCCTCAAT AAAGCTTGCC TTGAGTGCTT CAAGTAGTGT
    10981 GTGCCCGTCT GTTGTGTGAC TCTGGTAACT AGAGATCCCT CAGACCCTTT TAGTCAGTGT
    11041 GGAAAATCTC TAGCAGTGCC GCCCGAACAG GGACTTGAAA GCGAAAGGGA AACCAGAGGA
    11101 GCTCTCTCGA CGCAGGACTC GGCTTGCTGA AGCGCGCACG GCAAGAGGCG AAGGGCGGCG
    11161 ACTGGTGAGT ACGCCAAAAA TTTTGACTAG CGGAAGGCTA GAAGGAAGAA GATGGTGCCA
    11221 GAGCGTCAGT ATTAAGCGGG GGAAAATTAG ATCGCGAAGG GAAAAAATTC GGTTAAGGCC
    11281 AGGCCGAGTA ATACCGGGGG AGATTAGATC GCGATGGGAA AAATTCGGTT AAGGCCAGGG
    11341 GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC
    11401 GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA
    11461 CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC
    11521 CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA
    11581 GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGGCCG CTGATCTTCA
    11641 GACCTGGAGG AGGAGATATG AGGGACAATT GGAGAAGTGA ATTATATAAA TATAAAGTAG
    11701 TAAAAATTGA ACCATTAGGA GTAGCACCCA CCAAGGCAAA GAGAAGAGTG GTGCAGAGAG
    11761 AAAAAAGAGC AGTGGGAATA GGAGCTTTGT TCCTTGGGTT CTTGGGAGCA GCAGGAAGCA
    11821 CTATGGGCGC AGCGTCAATG ACGCTGACGG TACAGGCCAG ACAATTATTG TCTGGTATAG
    11881 TGCAGCAGCA GAACAATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG TTGCAACTCA
    11941 CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA TACCTAAAGG
    12001 ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC ACTGCTGTGC
    12061 CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC ACGACCTGGA
    12121 TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA ATTGAAGAAT
    12181 CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA TGGGCAAGTT
    12241 TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC ATAATGATAG
    12301 TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG AATAGAGTTA
    12361 GGCAGGGATA TTCACCATTA TCGTTTCAGA CCCACCTCCC AACCCCGAGG GGACCCGACA
    12421 GGCCCGAAGG AATAGAAGAA GAAGGTGGAG AGAGAGACAG AGACAGATCC ATTCGATTAG
    12481 TGAACGGATC GGCACTGCGT GCGCCAATTC TGCAGACAAA TGGCAGTATT CATCCACAAT
    12541 TTTAAAAGAA AAGGGGGGAT TGGGGGGTAC AGTGCAGGGG AAAGAATAGT AGACATAATA
    12601 GCAACAGACA TACAAACTAA AGAATTACAA AAACAAATTA CAAAAATTCA AAATTTTCGG
    12661 GTTTATTACA GGGACAGCAG AGATCCAGTT TGGTTAGTAC CGGGCCCGCT CTAGTCCGGA
    12721 ATCAGTCCTG CTCCTCGGCC ACGAAGTGCA CGCAGTTGCC GGCCGGGTCG CGCAGGGCGA
    12781 ACTCCCGCCC CCACGGCTGC TCGCCGATCT CGGTCATGGC CGGCCCGGAG GCGTCCCGGA
    12841 AGTTCGTGGA CACGACCTCC GACCACTCGG CGTACAGCTC GTCCAGGCCG CGCACCCACA
    12901 CCCAGGCCAG GGTGTTGTCC GGCACCACCT GGTCCTGGAC CGCGCTGATG AACAGGGTCA
    12961 CGTCGTCCCG GACCACACCG GCGAAGTCGT CCTCCACGAA GTCCCGGGAG AACCCGAGCC
    13021 GGTCGGTCCA GAACTCGACC GCTCCGGCGA CGTCGCGCGC GGTGAGCACC GGAACGGCAC
    13081 TGGTCAACTT GGCCATGGTG G
//
