LOCUS       pv6_CBX7_2xChromo       7437 bp ds-DNA     circular     09-JUN-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          358..379
                     /label="usL1b"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    409..421
                     /label="inverted_repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    409..442
                     /label="L1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(430..442)
                     /label="inverted_repeat"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    445..826
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        445..2161
                     /label="CAG (not sequenced!)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(596..619)
                     /label="CAG 5' r"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     TATA_signal     1077..1084
                     /label="TATAAA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     TATA_signal     1091..1130
                     /label="replace with 2xTetO"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     TATA_signal     1106..1113
                     /label="transcript start"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2167..2218
                     /label="BIOtag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2171..3628
                     /label="Translation 2171-3628"
     misc_feature    2222..2242
                     /label="TEV cleavage"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2249..2278
                     /label="GibsonF"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    complement(2279..2297)
                     /label="rev"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2279..2503
                     /label="Cbx7_Chromo"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2279..3628
                     /label="CBX7.2xChromo-EGFP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    complement(2504..2533)
                     /label="GibsonR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     nls             2540..2566
                     /label="nls"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2579..2860
                     /label="Cbx7_Chromo_v2"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2912..3625
                     /label="eGFP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          3668..3693
                     /label="tb288"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     IRES            3673..4213
                     /label="IRES"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4214..4825
                     /label="puroR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          4974..4996
                     /label="polAfwd"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_site      4976..5133
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5149..5182)
                     /label="<lox511"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5362..5382)
                     /label="ds1Lb"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctcgagccat aacttcgtat
      421 aatgtatAct atacgaagtt atatcgGACA TTGATTATTG ACTAGTTATT AATAGTAATC
      481 AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT
      541 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA
      601 TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
      661 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
      721 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT
      781 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC
      841 CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT
      901 TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGCGC GCGCCAGGCG
      961 GGGCGGGGCG GGGCGAGGGG CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC
     1021 AGAGCGGCGC GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG CGGCCCTATA
     1081 AAAAGCGAAG CGCGCGGCGG GCGGGAGTCG CTGCGTTGCC TTCGCCCCGT GCCCCGCTCC
     1141 GCGCCGCCTC GCGCCGCCCG CCCCGGCTCT GACTGACCGC GTTACTCCCA CAGGTGAGCG
     1201 GGCGGGACGG CCCTTCTCCT CCGGGCTGTA ATTAGCGCTT GGTTTAATGA CGGCTCGTTT
     1261 CTTTTCTGTG GCTGCGTGAA AGCCTTAAAG GGCTCCGGGA GGGCCCTTTG TGCGGGGGGG
     1321 AGCGGCTCGG GGGGTGCGTG CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG CGGCCCGCGC
     1381 TGCCCGGCGG CTGTGAGCGC TGCGGGCGCG GCGCGGGGCT TTGTGCGCTC CGCGTGTGCG
     1441 CGAGGGGAGC GCGGCCGGGG GCGGTGCCCC GCGGTGCGGG GGGGCTGCGA GGGGAACAAA
     1501 GGCTGCGTGC GGGGTGTGTG CGTGGGGGGG TGAGCAGGGG GTGTGGGCGC GGCGGTCGGG
     1561 CTGTAACCCC CCCCTGCACC CCCCTCCCCG AGTTGCTGAG CACGGCCCGG CTTCGGGTGC
     1621 GGGGCTCCGT GCGGGGCGTG GCGCGGGGCT CGCCGTGCCG GGCGGGGGGT GGCGGCAGGT
     1681 GGGGGTGCCG GGCGGGGCGG GGCCGCCTCG GGCCGGGGAG GGCTCGGGGG AGGGGCGCGG
     1741 CGGCCCCGGA GCGCCGGCGG CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG
     1801 TAATCGTGCG AGAGGGCGCA GGGACTTCCT TTGTCCCAAA TCTGGCGGAG CCGAAATCTG
     1861 GGAGGCGCCG CCGCACCCCC TCTAGCGGGC GCGGGCGAAG CGGTGCGGCG CCGGCAGGAA
     1921 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCATCTCCA
     1981 GCCTCGGGGC TGCCGCAGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC
     2041 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC
     2101 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA
     2161 GAATTcTAGC ATGGCTGGTG GCCTGAATGA CATCTTTGAG GCCCAGAAGA TCGAGTGGCA
     2221 TGAGAACCTG TACTTCCAGG GAgtcgacga aGAGTCTGGT TCTGTTtctg gtggtgatGA
     2281 GCTGTCAGCC ATAGGCGAGC AGGTGTTTGC GGTGGAGAGC ATCCGGAAGA AGCGCGTGCG
     2341 GAAGGGCAAA GTTGAATATC TGGTGAAGTG GAAAGGATGG CCCCCCAAGT ATAGCACCTG
     2401 GGAGCCAGAG GAGCACATCT TGGACCCTCG CCTTGTCATG GCCTACGAGG AGAAGGAGGA
     2461 GAGAGACCGA GCCTCGGGGT ATAGGAAGAG AGGTCCGAAA CCCatcggtg gcggaggctc
     2521 tggaggtgga ggctccgcaC CCCCCAAAAA GAAACGCAAG GTTGGAtacc gggatccaGG
     2581 TTCTGTTtct ggtggtgatG AGCTGTCAGC CATAGGCGAG CAGGTGTTTG CGGTGGAGAG
     2641 CATCCGGAAG AAGCGCGTGC GGAAGGGCAA AGTTGAATAT CTGGTGAAGT GGAAAGGATG
     2701 GCCCCCCAAG TATAGCACCT GGGAGCCAGA GGAGCACATC TTGGACCCTC GCCTTGTCAT
     2761 GGCCTACGAG GAGAAGGAGG AGAGAGACCG AGCCTCGGGG TATAGGAAGA GAGGTCCGAA
     2821 ACCCatcggt ggcggaggct ctggaggtgg aggctccgca CCCCCCAAAA AGAAACGCAA
     2881 GGTTGGATAC CGGGATCCAc cggtcgccac cgtgagcaag ggcgaggagc tgttcaccgg
     2941 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     3001 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     3061 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     3121 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     3181 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     3241 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     3301 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     3361 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     3421 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     3481 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     3541 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     3601 tctcggcatg gacgagctgt acaagtaaag cgAGctcgac aGATCTAGGA GCGGTTAACG
     3661 AGGTAGCGGC CGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT ATATGTTATT
     3721 TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC CTGTCTTCTT
     3781 GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC TGTTGAATGT
     3841 CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT
     3901 TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA AGCCACGTGT
     3961 ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT GGATAGTTGT
     4021 GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG ATGCCCAGAA
     4081 GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA CATGTGTTTA
     4141 GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG ACGTGGTTTT CCTTTGAAAA
     4201 ACACGATGAT AATATGGCCA CAACCATGAC CGAGTACAAG CCCACGGTGC GCCTCGCCAC
     4261 CCGCGACGAC GTCCCCCGGG CCGTACGCAC CCTCGCCGCC GCGTTCGCCG ACTACCCCGC
     4321 CACGCGCCAC ACCGTCGACC CGGACCGCCA CATCGAGCGG GTCACCGAGC TGCAAGAACT
     4381 CTTCCTCACG CGCGTCGGGC TCGACATCGG CAAGGTGTGG GTCGCGGACG ACGGCGCCGC
     4441 GGTGGCGGTC TGGACCACGC CGGAGAGCGT CGAAGCGGGG GCGGTGTTCG CCGAGATCGG
     4501 CCCGCGCATG GCCGAGTTGA GCGGTTCCCG GCTGGCCGCG CAGCAACAGA TGGAAGGCCT
     4561 CCTGGCGCCG CACCGGCCCA AGGAGCCCGC GTGGTTCCTG GCCACCGTCG GCGTCTCGCC
     4621 CGACCACCAG GGCAAGGGTC TGGGCAGCGC CGTCGTGCTC CCCGGAGTGG AGGCGGCCGA
     4681 GCGCGCCGGG GTGCCCGCCT TCCTGGAGAC CTCCGCGCCC CGCAACCTCC CCTTCTACGA
     4741 GCGGCTCGGC TTCACCGTCA CCGCCGACGT CGAGGTGCCC GAAGGACCGC GCACCTGGTG
     4801 CATGACCCGC AAGCCCGGTG CCTGACGCCC GCCCCACGAC CCGCAGCGCC CGACCGAAAG
     4861 GAGCGCAGTG CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG
     4921 AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT
     4981 CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT
     5041 CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT
     5101 CATCAATGTA TCTTATCATG TCTGTATACC GTGGATccgg ccggccccat aacttcgtat
     5161 agTatacatt atacgaagtt atcccctgca ggcatgcaag cttggcgtaa tcatggtcat
     5221 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5281 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5341 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5401 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5461 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5521 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5581 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5641 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5701 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5761 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5821 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5881 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     5941 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6001 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6061 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6121 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6181 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6241 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6301 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6361 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6421 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6481 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6541 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6601 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6661 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6721 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6781 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6841 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6901 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6961 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7021 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7081 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7141 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7201 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7261 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7321 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7381 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//