LOCUS       pv6_Taf3_2xPHD          7533 bp ds-DNA     circular     17-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          358..379
                     /label="usL1b"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer_bind     378..395
                     /label="M13-fwd"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    409..421
                     /label="inverted_repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    409..442
                     /label="L1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(430..442)
                     /label="inverted_repeat"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    445..826
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        445..2161
                     /label="CAG (not sequenced!)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(596..619)
                     /label="CAG 5' r"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     TATA_signal     1077..1084
                     /label="TATAAA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     TATA_signal     1091..1130
                     /label="replace with 2xTetO"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     TATA_signal     1106..1113
                     /label="transcript start"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2167..2218
                     /label="BIOtag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2171..3724
                     /label="Translation 2171-3724"
     misc_feature    2222..2242
                     /label="TEV cleavage"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2249..2278
                     /label="GibsonF"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2279..2554
                     /label="Taf3.PHD domain"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    2279..3724
                     /label="Taf3.2xPHD-EGFP"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    2555..2644
                     /label="30mer linker"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2645..2920
                     /label="Taf3.PHD domain"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    complement(2921..2950)
                     /label="GibsonR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     nls             2957..2983
                     /label="nls"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             3008..3721
                     /label="eGFP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          3764..3789
                     /label="tb288"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     IRES            3769..4309
                     /label="IRES"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             4310..4921
                     /label="puroR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          5070..5092
                     /label="polAfwd"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_site      5072..5229
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5245..5278)
                     /label="<lox511"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     complement(5308..5328)
                     /label="M13-rev"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          complement(5458..5478)
                     /label="ds1Lb"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     CDS             complement(6476..7135)
                     /label="AmpR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctcgagccat aacttcgtat
      421 aatgtatAct atacgaagtt atatcgGACA TTGATTATTG ACTAGTTATT AATAGTAATC
      481 AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT
      541 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA
      601 TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
      661 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
      721 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT
      781 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC
      841 CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT
      901 TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGCGC GCGCCAGGCG
      961 GGGCGGGGCG GGGCGAGGGG CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC
     1021 AGAGCGGCGC GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG CGGCCCTATA
     1081 AAAAGCGAAG CGCGCGGCGG GCGGGAGTCG CTGCGTTGCC TTCGCCCCGT GCCCCGCTCC
     1141 GCGCCGCCTC GCGCCGCCCG CCCCGGCTCT GACTGACCGC GTTACTCCCA CAGGTGAGCG
     1201 GGCGGGACGG CCCTTCTCCT CCGGGCTGTA ATTAGCGCTT GGTTTAATGA CGGCTCGTTT
     1261 CTTTTCTGTG GCTGCGTGAA AGCCTTAAAG GGCTCCGGGA GGGCCCTTTG TGCGGGGGGG
     1321 AGCGGCTCGG GGGGTGCGTG CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG CGGCCCGCGC
     1381 TGCCCGGCGG CTGTGAGCGC TGCGGGCGCG GCGCGGGGCT TTGTGCGCTC CGCGTGTGCG
     1441 CGAGGGGAGC GCGGCCGGGG GCGGTGCCCC GCGGTGCGGG GGGGCTGCGA GGGGAACAAA
     1501 GGCTGCGTGC GGGGTGTGTG CGTGGGGGGG TGAGCAGGGG GTGTGGGCGC GGCGGTCGGG
     1561 CTGTAACCCC CCCCTGCACC CCCCTCCCCG AGTTGCTGAG CACGGCCCGG CTTCGGGTGC
     1621 GGGGCTCCGT GCGGGGCGTG GCGCGGGGCT CGCCGTGCCG GGCGGGGGGT GGCGGCAGGT
     1681 GGGGGTGCCG GGCGGGGCGG GGCCGCCTCG GGCCGGGGAG GGCTCGGGGG AGGGGCGCGG
     1741 CGGCCCCGGA GCGCCGGCGG CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG
     1801 TAATCGTGCG AGAGGGCGCA GGGACTTCCT TTGTCCCAAA TCTGGCGGAG CCGAAATCTG
     1861 GGAGGCGCCG CCGCACCCCC TCTAGCGGGC GCGGGCGAAG CGGTGCGGCG CCGGCAGGAA
     1921 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCATCTCCA
     1981 GCCTCGGGGC TGCCGCAGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC
     2041 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC
     2101 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA
     2161 GAATTcTAGC ATGGCTGGTG GCCTGAATGA CATCTTTGAG GCCCAGAAGA TCGAGTGGCA
     2221 TGAGAACCTG TACTTCCAGG GAgtcgacga aGAGTCTGGT TCTGTTtctg gtggtgatAG
     2281 TGCCAAAGCC CCCGTGAGGA GCGTCGTGAC TGAGACGGTC AGCACTTATG TGATCCGTGA
     2341 TGAGTGGGGC AATCAGATCT GGATCTGTCC AGGGTGCAAC AAGCCTGATG ACGGGAGCCC
     2401 GATGATTGGC TGTGACGACT GTGACGATTG GTACCACTGG CCCTGTGTGG GAATCATGGC
     2461 TGCTCCTCCA GAAGAGATGC AGTGGTTCTG CCCGAAGTGT GCCAACAAGA TAAAGAAAGA
     2521 CAAGAAGCAC AAGAAGAGGA AGCACCGTGC GCACGGTGGT GGCGGATCAG AAGGAGGCGG
     2581 TAGCGGGGGC CCTGGTTCGG GAGGGGAAGG TTCTGCTGGG GGAGGGAGCG CTGGCGGGGG
     2641 GTCTAGTGCA AAGGCACCTG TCCGAAGTGT CGTCACAGAA ACTGTGTCTA CTTATGTCAT
     2701 CAGAGACGAA TGGGGAAATC AGATTTGGAT CTGTCCCGGC TGCAATAAGC CAGATGACGG
     2761 ATCACCTATG ATAGGATGTG ACGACTGCGA TGACTGGTAT CACTGGCCTT GCGTTGGGAT
     2821 CATGGCCGCC CCCCCCGAAG AGATGCAATG GTTTTGCCCC AAATGCGCCA ATAAAATAAA
     2881 AAAGGACAAG AAACATAAGA AACGCAAGCA TCGCGCTCAC atcggtggcg gaggctctgg
     2941 aggtggaggc tccgcaCCCC CCAAAAAGAA ACGCAAGGTT GGAtaccggg atccaccggt
     3001 cgccaccgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     3061 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     3121 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     3181 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     3241 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     3301 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     3361 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     3421 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     3481 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     3541 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     3601 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat
     3661 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     3721 gtaaagcgAG ctcgacaGAT CTAGGAGCGG TTAACGAGGT AGCGGCCGCC GAAGCCGCTT
     3781 GGAATAAGGC CGGTGTGCGT TTGTCTATAT GTTATTTTCC ACCATATTGC CGTCTTTTGG
     3841 CAATGTGAGG GCCCGGAAAC CTGGCCCTGT CTTCTTGACG AGCATTCCTA GGGGTCTTTC
     3901 CCCTCTCGCC AAAGGAATGC AAGGTCTGTT GAATGTCGTG AAGGAAGCAG TTCCTCTGGA
     3961 AGCTTCTTGA AGACAAACAA CGTCTGTAGC GACCCTTTGC AGGCAGCGGA ACCCCCCACC
     4021 TGGCGACAGG TGCCTCTGCG GCCAAAAGCC ACGTGTATAA GATACACCTG CAAAGGCGGC
     4081 ACAACCCCAG TGCCACGTTG TGAGTTGGAT AGTTGTGGAA AGAGTCAAAT GGCTCTCCTC
     4141 AAGCGTATTC AACAAGGGGC TGAAGGATGC CCAGAAGGTA CCCCATTGTA TGGGATCTGA
     4201 TCTGGGGCCT CGGTGCACAT GCTTTACATG TGTTTAGTCG AGGTTAAAAA ACGTCTAGGC
     4261 CCCCCGAACC ACGGGGACGT GGTTTTCCTT TGAAAAACAC GATGATAATA TGGCCACAAC
     4321 CATGACCGAG TACAAGCCCA CGGTGCGCCT CGCCACCCGC GACGACGTCC CCCGGGCCGT
     4381 ACGCACCCTC GCCGCCGCGT TCGCCGACTA CCCCGCCACG CGCCACACCG TCGACCCGGA
     4441 CCGCCACATC GAGCGGGTCA CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA
     4501 CATCGGCAAG GTGTGGGTCG CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA
     4561 GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG
     4621 TTCCCGGCTG GCCGCGCAGC AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA
     4681 GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG
     4741 CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT
     4801 GGAGACCTCC GCGCCCCGCA ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC
     4861 CGACGTCGAG GTGCCCGAAG GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG
     4921 ACGCCCGCCC CACGACCCGC AGCGCCCGAC CGAAAGGAGC GCAGTGCTAC GAGATTTCGA
     4981 TTCCACCGCC GCCTTCTATG AAAGGTTGGG CTTCGGAATC GTTTTCCGGG ACGCCGGCTG
     5041 GATGATCCTC CAGCGCGGGG ATCTCATGCT GGAGTTCTTC GCCCACCCCA ACTTGTTTAT
     5101 TGCAGCTTAT AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT
     5161 TTTTTCACTG CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG
     5221 TATACCGTGG ATccggccgg ccccataact tcgtatagTa tacattatac gaagttatcc
     5281 cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     5341 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     5401 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     5461 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     5521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     5581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     5641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     5701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     5761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     5821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     5881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     5941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     6001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     6061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     6121 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     6181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     6241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     6301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     6361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     6421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     6481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     6541 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     6601 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     6661 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     6721 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     6781 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     6841 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     6901 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     6961 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     7021 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     7081 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     7141 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     7201 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     7261 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     7321 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     7381 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     7441 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     7501 ataaaaatag gcgtatcacg aggccctttc gtc
//