LOCUS       pv6_CBX7_Chromo_-_TAF3_ 7482 bp ds-DNA     circular     20-MAR-2020
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          358..379
                     /label="usL1b = tb079"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    409..421
                     /label="inverted_repeat"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    409..442
                     /label="L1"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    complement(430..442)
                     /label="inverted_repeat"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    445..826
                     /label="CMV enhancer"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     promoter        445..2161
                     /label="CAG (not sequenced!)"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(596..619)
                     /label="CAG 5' r"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     TATA_signal     1077..1084
                     /label="TATAAA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     TATA_signal     1091..1130
                     /label="replace with 2xTetO"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     TATA_signal     1106..1113
                     /label="transcript start"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2128..2146
                     /label="rv040"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2167..2218
                     /label="BIOtag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             2171..3673
                     /label="Translation 2171-3673"
     misc_feature    2222..2242
                     /label="TEV cleavage"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    2249..2278
                     /label="GibsonF"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2252..2271
                     /label="rv043"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2279..2503
                     /label="Cbx7.Chromo(extended_geneartopt)"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    2504..2593
                     /label="30mer_linker"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2594..2869
                     /label="Taf3.PHD_mut"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    complement(2870..2899)
                     /label="GibsonR"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(2879..2896)
                     /label="rv044"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     nls             2906..2932
                     /label="nls"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             2957..3670
                     /label="eGFP"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          2970..2989
                     /label="rv001"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3254..3275
                     /label="tb175"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(3419..3440)
                     /label="tb176"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          3713..3738
                     /label="tb288"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     IRES            3718..4258
                     /label="IRES"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3982..3998
                     /label="rv022"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     CDS             4259..4870
                     /label="puroR"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          5019..5041
                     /label="polAfwd"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     polyA_site      5021..5178
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    complement(5194..5227)
                     /label="<lox511"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(5407..5427)
                     /label="ds1Lb"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt ctcgagccat aacttcgtat
      421 aatgtatAct atacgaagtt atatcgGACA TTGATTATTG ACTAGTTATT AATAGTAATC
      481 AATTACGGGG TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT
      541 AAATGGCCCG CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA
      601 TGTTCCCATA GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG
      661 GTAAACTGCC CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA
      721 CGTCAATGAC GGTAAATGGC CCGCCTGGCA TTATGCCCAG TACATGACCT TATGGGACTT
      781 TCCTACTTGG CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC
      841 CACGTTCTGC TTCACTCTCC CCATCTCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT
      901 TATTTTTTAA TTATTTTGTG CAGCGATGGG GGCGGGGGGG GGGGGGGCGC GCGCCAGGCG
      961 GGGCGGGGCG GGGCGAGGGG CGGGGCGGGG CGAGGCGGAG AGGTGCGGCG GCAGCCAATC
     1021 AGAGCGGCGC GCTCCGAAAG TTTCCTTTTA TGGCGAGGCG GCGGCGGCGG CGGCCCTATA
     1081 AAAAGCGAAG CGCGCGGCGG GCGGGAGTCG CTGCGTTGCC TTCGCCCCGT GCCCCGCTCC
     1141 GCGCCGCCTC GCGCCGCCCG CCCCGGCTCT GACTGACCGC GTTACTCCCA CAGGTGAGCG
     1201 GGCGGGACGG CCCTTCTCCT CCGGGCTGTA ATTAGCGCTT GGTTTAATGA CGGCTCGTTT
     1261 CTTTTCTGTG GCTGCGTGAA AGCCTTAAAG GGCTCCGGGA GGGCCCTTTG TGCGGGGGGG
     1321 AGCGGCTCGG GGGGTGCGTG CGTGTGTGTG TGCGTGGGGA GCGCCGCGTG CGGCCCGCGC
     1381 TGCCCGGCGG CTGTGAGCGC TGCGGGCGCG GCGCGGGGCT TTGTGCGCTC CGCGTGTGCG
     1441 CGAGGGGAGC GCGGCCGGGG GCGGTGCCCC GCGGTGCGGG GGGGCTGCGA GGGGAACAAA
     1501 GGCTGCGTGC GGGGTGTGTG CGTGGGGGGG TGAGCAGGGG GTGTGGGCGC GGCGGTCGGG
     1561 CTGTAACCCC CCCCTGCACC CCCCTCCCCG AGTTGCTGAG CACGGCCCGG CTTCGGGTGC
     1621 GGGGCTCCGT GCGGGGCGTG GCGCGGGGCT CGCCGTGCCG GGCGGGGGGT GGCGGCAGGT
     1681 GGGGGTGCCG GGCGGGGCGG GGCCGCCTCG GGCCGGGGAG GGCTCGGGGG AGGGGCGCGG
     1741 CGGCCCCGGA GCGCCGGCGG CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG
     1801 TAATCGTGCG AGAGGGCGCA GGGACTTCCT TTGTCCCAAA TCTGGCGGAG CCGAAATCTG
     1861 GGAGGCGCCG CCGCACCCCC TCTAGCGGGC GCGGGCGAAG CGGTGCGGCG CCGGCAGGAA
     1921 GGAAATGGGC GGGGAGGGCC TTCGTGCGTC GCCGCGCCGC CGTCCCCTTC TCCATCTCCA
     1981 GCCTCGGGGC TGCCGCAGGG GGACGGCTGC CTTCGGGGGG GACGGGGCAG GGCGGGGTTC
     2041 GGCTTCTGGC GTGTGACCGG CGGCTCTAGA GCCTCTGCTA ACCATGTTCA TGCCTTCTTC
     2101 TTTTTCCTAC AGCTCCTGGG CAACGTGCTG GTTGTTGTGC TGTCTCATCA TTTTGGCAAA
     2161 GAATTcTAGC ATGGCTGGTG GCCTGAATGA CATCTTTGAG GCCCAGAAGA TCGAGTGGCA
     2221 TGAGAACCTG TACTTCCAGG GAgtcgacga aGAGTCTGGT TCTGTTtctg gtggtgatGA
     2281 GCTGTCAGCC ATtGGCGAGC AGGTGTTTGC GGTGGAGAGC ATCCGGAAGA AGCGCGTGCG
     2341 GAAGGGCAAA GTTGAATATC TGGTGAAGTG GAAAGGATGG CCgCCCAAGT ATAGCACCTG
     2401 GGAGCCtGAG GAGCAtATCc TcGACCCTCG CtTgGTgATG GCCTACGAGG AGAAGGAGGA
     2461 GAGAGACaGg GCCTCGGGGT ATAGGAAGAG AGGTCCGAAA CCCggtggtg gcggatcaga
     2521 aggaggcggt agcgggggcc ctggttcggg aggggaaggt tctgctgggg gagggagcgc
     2581 tggcgggggg tctAGTGCAA AGGCACCTGT CCGAAGTGTC GTCACAGAAA CTGTGTCTAC
     2641 TTATGTCATC AGAGACGAAT GGGGAAATCA GATTTGGATC TGTCCCGGCT GCAATAAGCC
     2701 AGATGACGGA TCACCTATGA TAGGATGTGA CGACTGCGAT gccgccTATC ACTGGCCTTG
     2761 CGTTGGGATC ATGGCCGCCC CCCCCGAAGA GATGCAATGG TTTTGCCCCA AATGCGCCAA
     2821 TAAAATAAAA AAGGACAAGA AACATAAGAA ACGCAAGCAT CGCGCTCACa tcggtggcgg
     2881 aggctctgga ggtggaggct ccgcaCCCCC CAAAAAGAAA CGCAAGGTTG GAtaccggga
     2941 tccaccggtc gccaccgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     3001 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     3061 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     3121 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     3181 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     3241 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     3301 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     3361 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     3421 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     3481 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     3541 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg
     3601 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     3661 gctgtacaag taaagcgAGc tcgacaGATC TAGGAGCGGT TAACGAGGTA GCGGCCGCCG
     3721 AAGCCGCTTG GAATAAGGCC GGTGTGCGTT TGTCTATATG TTATTTTCCA CCATATTGCC
     3781 GTCTTTTGGC AATGTGAGGG CCCGGAAACC TGGCCCTGTC TTCTTGACGA GCATTCCTAG
     3841 GGGTCTTTCC CCTCTCGCCA AAGGAATGCA AGGTCTGTTG AATGTCGTGA AGGAAGCAGT
     3901 TCCTCTGGAA GCTTCTTGAA GACAAACAAC GTCTGTAGCG ACCCTTTGCA GGCAGCGGAA
     3961 CCCCCCACCT GGCGACAGGT GCCTCTGCGG CCAAAAGCCA CGTGTATAAG ATACACCTGC
     4021 AAAGGCGGCA CAACCCCAGT GCCACGTTGT GAGTTGGATA GTTGTGGAAA GAGTCAAATG
     4081 GCTCTCCTCA AGCGTATTCA ACAAGGGGCT GAAGGATGCC CAGAAGGTAC CCCATTGTAT
     4141 GGGATCTGAT CTGGGGCCTC GGTGCACATG CTTTACATGT GTTTAGTCGA GGTTAAAAAA
     4201 CGTCTAGGCC CCCCGAACCA CGGGGACGTG GTTTTCCTTT GAAAAACACG ATGATAATAT
     4261 GGCCACAACC ATGACCGAGT ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC
     4321 CCGGGCCGTA CGCACCCTCG CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT
     4381 CGACCCGGAC CGCCACATCG AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT
     4441 CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC
     4501 CACGCCGGAG AGCGTCGAAG CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA
     4561 GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG
     4621 GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC CGTCGGCGTC TCGCCCGACC ACCAGGGCAA
     4681 GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC
     4741 CGCCTTCCTG GAGACCTCCG CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC
     4801 CGTCACCGCC GACGTCGAGG TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC
     4861 CGGTGCCTGA CGCCCGCCCC ACGACCCGCA GCGCCCGACC GAAAGGAGCG CAGTGCTACG
     4921 AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA
     4981 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA
     5041 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA
     5101 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA
     5161 TCATGTCTGT ATACCGTGGA Tccggccggc cccataactt cgtatagTat acattatacg
     5221 aagttatccc ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     5281 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     5341 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     5401 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     5461 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     5521 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5581 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5641 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5701 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5761 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5821 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5881 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5941 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     6001 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     6061 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     6121 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     6181 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     6241 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     6301 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     6361 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     6421 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     6481 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6541 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6601 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6661 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6721 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6781 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6841 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6901 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6961 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     7021 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     7081 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     7141 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     7201 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     7261 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     7321 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     7381 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     7441 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//