LOCUS       Exported                7663 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(29..47)
                     /label=GEX3-rev-25
                     /note="color: #d59687; direction: LEFT"
     misc_feature    complement(57..76)
                     /label=GEX3-rev
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    326..347
                     /label=rv060
                     /note="color: #75c6a9"
     misc_feature    327..346
                     /label=pUCM13-52
                     /note="color: #85dae9"
     misc_feature    358..379
                     /label=usL1b
                     /note="color: #84b0dc"
     misc_feature    359..375
                     /label=M13-40
                     /note="color: #c7b0e3"
     misc_feature    371..420
                     /label=rv141
                     /note="color: #b1ff67"
     misc_feature    complement(371..420)
                     /label=rv142
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    378..394
                     /label=M13
                     /note="color: #9eafd2"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    398..432
                     /label=L1
                     /note="color: #75c6a9"
     protein_bind    complement(398..431)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     promoter        434..2058
                     /label=CAG (not sequenced!)
                     /note="color: #75c6a9"
     misc_feature    434..815
                     /label=CMV enhancer
                     /note="color: #9eafd2"
     enhancer        436..815
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    complement(585..608)
                     /label=CAG 5' r
                     /note="color: #f58a5e; direction: LEFT"
     promoter        817..1092
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     TATA_signal     1066..1073
                     /label=TATAAA
                     /note="color: #75c6a9"
     TATA_signal     1080..1119
                     /label=replace with 2xTetO
                     /note="color: #ffef86"
     intron          1093..2101
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     TATA_signal     1095..1102
                     /label=transcript start
                     /note="color: #f58a5e"
     misc_feature    1981..2058
                     /label=homologousRegion
                     /note="color: #ff9ccd"
     misc_feature    2025..2929
                     /label=seq1
                     /note="color: #faac61"
     misc_feature    2025..2044
                     /label=rp022_CR002_fwd
                     /note="color: #f58a5e"
     misc_feature    2025..2044
                     /label=fwd better
                     /note="color: #85dae9"
     misc_feature    2059..2810
                     /label=newfragment
                     /note="color: #ff9ccd"
     misc_feature    2117..2135
                     /label=rv040
                     /note="color: #f58a5e"
     misc_feature    2176..2187
                     /label=Kosak_consensus
                     /note="color: #faac61"
     regulatory      2179..2188
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2185..3879
                     /label=Translation 2185-3879
                     /note="color: #993366; direction: RIGHT"
     CDS             2188..2211
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    2215..2244
                     /label=GibsonF
                     /note="color: #ff9ccd"
     misc_feature    2218..2244
                     /label=rv164_gibsonF_fwd
                     /note="color: #c7b0e3"
     misc_feature    2218..2237
                     /label=rv043
                     /note="color: #b7e6d7"
     gene            2245..2439
                     /label=Cbx1_Chromo
                     /note="color: #c6c9d1; direction: RIGHT"
     misc_feature    complement(2449..2466)
                     /label=rv044
                     /note="color: #84b0dc; direction: LEFT"
     CDS             2479..2499
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     gene            2515..2709
                     /label=Cbx1_Chromo
                     /note="color: #c7b0e3; direction: RIGHT"
     misc_feature    complement(2728..2754)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2728..2747
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2737..2754)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    2764..2790
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2767..2787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    complement(2793..2830)
                     /label=rp023_CR2_13XLrev
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(2793..2830)
                     /label=GA rev pCR002
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(2793..2810)
                     /label=rev pCR002
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2803..2808
                     /label=intro site reader domain
                     /note="color: #75c6a9"
     misc_feature    2811..2845
                     /label=GA fwd, pv8 (rp012)
                     /note="color: #ff9ccd"
     misc_feature    complement(2811..2830)
                     /label=GA overhang
                     /note="color: #b4abac; direction: LEFT"
     gene            2851..3054
                     /label=13X-Linker
                     /note="color: #f58a5e; direction: RIGHT"
     misc_feature    complement(3054..3102)
                     /label=rp007_Link_BASU
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3054..3072)
                     /label=rev primer
                     /label=rev primer; BioID1
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    3073..3100
                     /label=rp006_BASU_f
                     /note="color: #ffef86"
     misc_feature    3073..3099
                     /label=HA Tag
                     /note="color: #d59687"
     CDS             3073..3099
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     gene            3100..3879
                     /label=BASU*
                     /note="color: #c6c9d1; direction: RIGHT"
     misc_feature    3340..3345
                     /label=Split Site
                     /note="color: #75c6a9"
     misc_feature    complement(3860..3912)
                     /label=GA rev, BASU (rp014)
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    complement(3860..3912)
                     /label=rp014_BASU_v8_r
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3860..3879)
                     /label=rev primer BASU
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    3871..3873
                     /label=E258S
                     /note="color: #f58a5e"
     misc_feature    3877..3879
                     /label=G2R
                     /note="color: #b1ff67"
     CDS             3880..3882
                     /label=Translation 3880-3882
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3894..3919
                     /label=tb288
                     /note="color: #84b0dc"
     CDS             3899..4439
                     /label=IRES
                     /note="color: #ff9ccd; direction: RIGHT"
     misc_feature    complement(3921..3939)
                     /label=IRES-rev
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    3977..4438
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    complement(3996..4014)
                     /label=rp019_IRESrev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    4163..4179
                     /label=rv022
                     /note="color: #75c6a9"
     CDS             4409..4442
                     /label=Translation 4409-4442
                     /note="color: #993366; direction: RIGHT"
     CDS             4440..5051
                     /label=puroR
                     /note="color: #84b0dc; direction: RIGHT"
     CDS             4440..5051
                     /label=Translation 4440-5051
                     /note="color: #993366; direction: RIGHT"
     CDS             4452..5051
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4584..4603
                     /label=rv117
                     /note="color: #75c6a9"
     misc_feature    complement(5077..5114)
                     /label=rv023
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(5134..5155)
                     /label=rv059
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    5200..5222
                     /label=polAfwd
                     /note="color: #c6c9d1"
     polyA_site      5202..5359
                     /label=SV40 polyA
                     /note="color: #f8d3a9"
     polyA_signal    5220..5341
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(5220..5237)
                     /label=EGFP-C-Rev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    5257..5278
                     /label=pCI-rev
                     /note="color: #f8d3a9"
     misc_feature    5311..5330
                     /label=EBV-rev
                     /note="color: #b4abac"
     misc_feature    5374..5408
                     /label=1L
                     /note="color: #b7e6d7"
     protein_bind    5375..5408
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(5442..5458)
                     /label=M13r
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(5442..5458)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    complement(5456..5475)
                     /label=LacOp-for
                     /note="color: #85dae9; direction: LEFT"
     protein_bind    5466..5482
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5490..5520)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5490 .. 5496 / #ffffff / -10
                        2: 5497 .. 5514 / #ffffff
                        3: 5515 .. 5520 / #ffffff / -35"
     misc_feature    complement(5490..5509)
                     /label=pUCM13-rev-157
                     /note="color: #84b0dc; direction: LEFT"
     protein_bind    5535..5556
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     misc_feature    complement(5588..5608)
                     /label=ds1Lb
                     /note="color: #f8d3a9; direction: LEFT"
     rep_origin      complement(5844..6432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6603..7463)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6603 .. 7394 / #ccffcc
                        2: 7395 .. 7463 / #ccffcc / signal sequence
                     Cleavage site after base 7394"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(6653..6672)
                     /label=AmpStop
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    7399..7418
                     /label=AmpStart
                     /note="color: #85dae9"
     promoter        complement(7464..7568)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7643..7661
                     /label=QE-for
                     /note="color: #d6b295"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtccata acttcgtata atgtatacta
      421 tacgaagtta tatcggacat tgattattga ctagttatta atagtaatca attacggggt
      481 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      541 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      601 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      661 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      721 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      781 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      841 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      901 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      961 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     1021 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     1081 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
     1141 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1201 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
     1261 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
     1321 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
     1381 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1441 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1501 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1561 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1621 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1681 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1741 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1801 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1861 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1921 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1981 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2041 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2101 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcattat
     2161 tgtttttcct cctttgccgc caccatggac tacaaagacg atgacgacaa ggatgaagag
     2221 tctggttctg tttctggtgg tgatgaagag gaatatgtgg tggaaaaagt tcttgatcgg
     2281 cgagttgtca agggcaaggt ggaatatctt ctaaagtgga agggtttctc agatgaggac
     2341 aacacttggg agccagaaga gaatctggat tgccctgacc ttattgctga gtttctacag
     2401 tcacagaaaa cagctcatga gacagataag tcagagggaa tcggtggcgg aggctctgga
     2461 ggtggaggct ccgcaccccc caaaaagaaa cgcaaggttg gataccggga tccagaagag
     2521 gaatatgtgg tggaaaaagt tcttgatcgg cgagttgtca agggcaaggt ggaatatctt
     2581 ctaaagtgga agggtttctc agatgaggac aacacttggg agccagaaga gaatctggat
     2641 tgccctgacc ttattgctga gtttctacag tcacagaaaa cagctcatga gacagataag
     2701 tcagagggac cggtcgccac cgtgagcatc ggtggcggag gctctggagg tggaggctcc
     2761 gcacccccca aaaagaaacg caaggttgga taccgggatc cacacgtggg taggagcggt
     2821 tcgttcggat cccatctttt ttcggaattc ggatccggtg gaggcgggtc tggaggcggg
     2881 ggtagtggcg ggggtggaag cgggggtgga ggcgggtcgg gtggcggagg tagcggaggc
     2941 ggtggaagtg gaggcggtgg aagtggtgga ggaggttctg gtggcggagg tagcggtggc
     3001 ggaggtagcg gaggcggtgg aagtggtggc ggaggtagcg gaggcggtgg atcggcgcgc
     3061 cgtttcaaga actacccata cgatgtgcct gactatgctg gcaagttgtc tgaatcagag
     3121 attaggtttg gactgaaaac ggaggtgatg ggccaacatc ttatctatca cgatgttttg
     3181 tccagcacac aaaagacagc ccatgaattg gcaaataaca atgcccccga gggtaccctc
     3241 gtggttgcag ataaacagac agccggtagg ggcgggatga gccgagtgtg gcattctcaa
     3301 gaaggaaacg gagtatggat gagtttgata ttgcgcccgg acattccgct ccaaaagacg
     3361 ccccaactta ccttgctggc agctgtagcg gtggttcagg gaatagaaga ggccgcgggc
     3421 atacagactg atataaagtg gccgaacgac atactcatta acgggaaaaa aacagtaggg
     3481 atacttaccg aaatgcaagc tgaggaggat cgagtgcgct ccgtgattat tggtattggc
     3541 attaatgtaa atcagcaacc caatgatttc cccgatgagc tcaaggacat tgcgacgagt
     3601 ctgtcccagg cagctgggga aaagatcgac agggcaggtg taatccagca tatcttgctt
     3661 tgtttcgaaa agcgataccg agactacatg acccacggtt tcacgcccat caagctcttg
     3721 tgggagtcat acgccctcgg tattggcacg aacatgaggg cgagaactct caacgggaca
     3781 ttttacggta aggcgttggg tattgacgac gaaggggtac ttttgttgga aactaatgaa
     3841 ggcattaaga agatatacag cgcggacatc agccttaggt gaaacgaggt agcggccgcc
     3901 gaagccgctt ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc
     3961 cgtcttttgg caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta
     4021 ggggtctttc ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag
     4081 ttcctctgga agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga
     4141 accccccacc tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg
     4201 caaaggcggc acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat
     4261 ggctctcctc aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta
     4321 tgggatctga tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa
     4381 acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata
     4441 tggccacaac catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc
     4501 cccgggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg
     4561 tcgacccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg
     4621 tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga
     4681 ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg
     4741 agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc
     4801 ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca
     4861 agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc
     4921 ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca
     4981 ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc
     5041 ccggtgcctg acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcagtgctac
     5101 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     5161 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
     5221 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5281 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     5341 atcatgtctg tataccgtgg atccggccgg ccccataact tcgtatagta tacattatac
     5401 gaagttatcc cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg
     5461 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     5521 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     5581 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     5641 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     5701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     5761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     5821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     5881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     5941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     6301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     6721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     6781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     6841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     6901 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     6961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7201 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc
//
