LOCUS       Exported                7726 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7726
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(29..47)
                     /label=GEX3-rev-25
                     /note="color: #d59687; direction: LEFT"
     misc_feature    complement(57..76)
                     /label=GEX3-rev
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    326..347
                     /label=rv060
                     /note="color: #75c6a9"
     misc_feature    327..346
                     /label=pUCM13-52
                     /note="color: #85dae9"
     misc_feature    358..379
                     /label=usL1b
                     /note="color: #84b0dc"
     misc_feature    359..375
                     /label=M13-40
                     /note="color: #c7b0e3"
     misc_feature    371..420
                     /label=rv141
                     /note="color: #b1ff67"
     misc_feature    complement(371..420)
                     /label=rv142
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    378..394
                     /label=M13
                     /note="color: #9eafd2"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    398..432
                     /label=L1
                     /note="color: #75c6a9"
     protein_bind    complement(398..431)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     promoter        434..2058
                     /label=CAG (not sequenced!)
                     /note="color: #75c6a9"
     misc_feature    434..815
                     /label=CMV enhancer
                     /note="color: #9eafd2"
     enhancer        436..815
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    complement(585..608)
                     /label=CAG 5' r
                     /note="color: #f58a5e; direction: LEFT"
     promoter        817..1092
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     TATA_signal     1066..1073
                     /label=TATAAA
                     /note="color: #75c6a9"
     TATA_signal     1080..1119
                     /label=replace with 2xTetO
                     /note="color: #ffef86"
     intron          1093..2101
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     TATA_signal     1095..1102
                     /label=transcript start
                     /note="color: #f58a5e"
     misc_feature    1981..2058
                     /label=homologousRegion
                     /note="color: #ff9ccd"
     misc_feature    2025..2992
                     /label=seq1
                     /note="color: #faac61"
     misc_feature    2025..2044
                     /label=fwd better
                     /note="color: #85dae9"
     misc_feature    2025..2044
                     /label=rp022_CR002_fwd
                     /note="color: #f58a5e"
     misc_feature    2117..2135
                     /label=rv040
                     /note="color: #f58a5e"
     misc_feature    2176..2187
                     /label=Kosak_consensus
                     /note="color: #faac61"
     regulatory      2179..2188
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2185..3942
                     /label=Translation 2185-3942
                     /note="color: #993366; direction: RIGHT"
     CDS             2188..2211
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    2215..2244
                     /label=GibsonF
                     /note="color: #ff9ccd"
     misc_feature    2218..2244
                     /label=rv164_gibsonF_fwd
                     /note="color: #c7b0e3"
     misc_feature    2218..2237
                     /label=rv043
                     /note="color: #b7e6d7"
     misc_feature    2225..2283
                     /label=rv131_Cbx7(CD)_fwd
                     /note="color: #75c6a9"
     misc_feature    2225..2244
                     /label=rv131_Cbx7(CD)_fwd
                     /note="color: #75c6a9"
     misc_feature    2245..2469
                     /label=Cbx7_Chromo
                     /note="color: #9eafd2"
     misc_feature    complement(2434..2489)
                     /label=rv132_Cbx7(CD)_rev
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(2470..2499)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2470..2489
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2479..2496)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(2488..2505)
                     /label=rev
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    2506..2532
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2509..2529
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    2520..2568
                     /label=rv187_Cbx7_GAfwd
                     /note="color: #c7b0e3"
     misc_feature    2546..2604
                     /label=rv131_Cbx7(CD)_fwd
                     /note="color: #75c6a9"
     misc_feature    complement(2755..2810)
                     /label=rv132_Cbx7(CD)_rev
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(2791..2817)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2791..2810
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2800..2817)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    2827..2853
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2830..2850
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    complement(2856..2893)
                     /label=rp023_CR2_13XLrev
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(2856..2893)
                     /label=GA rev pCR002
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(2856..2873)
                     /label=rev pCR002
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2866..2871
                     /label=intro site reader domain
                     /note="color: #75c6a9"
     misc_feature    2874..2908
                     /label=GA fwd, pv8 (rp012)
                     /note="color: #ff9ccd"
     misc_feature    complement(2874..2893)
                     /label=GA overhang
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2914..3117
                     /label=13X-Linker
                     /note="color: #f58a5e"
     misc_feature    complement(3117..3165)
                     /label=rp007_Link_BASU
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3117..3135)
                     /label=rev primer
                     /label=rev primer; BioID1
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    3136..3163
                     /label=rp006_BASU_f
                     /note="color: #ffef86"
     misc_feature    3136..3162
                     /label=HA Tag
                     /note="color: #d59687"
     CDS             3136..3162
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     gene            3163..3942
                     /label=BASU*
                     /note="color: #c6c9d1; direction: RIGHT"
     misc_feature    3403..3408
                     /label=Split Site
                     /note="color: #75c6a9"
     misc_feature    complement(3923..3975)
                     /label=GA rev, BASU (rp014)
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    complement(3923..3975)
                     /label=rp014_BASU_v8_r
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3923..3942)
                     /label=rev primer BASU
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    3934..3936
                     /label=E258S
                     /note="color: #f58a5e"
     misc_feature    3940..3942
                     /label=G2R
                     /note="color: #b1ff67"
     CDS             3943..3945
                     /label=Translation 3943-3945
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3957..3982
                     /label=tb288
                     /note="color: #84b0dc"
     misc_feature    3962..4502
                     /label=IRES
                     /note="color: #ff9ccd"
     misc_feature    complement(3984..4002)
                     /label=IRES-rev
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    4040..4501
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    complement(4059..4077)
                     /label=rp019_IRESrev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    4226..4242
                     /label=rv022
                     /note="color: #75c6a9"
     CDS             4472..4505
                     /label=Translation 4472-4505
                     /note="color: #993366; direction: RIGHT"
     CDS             4503..5114
                     /label=puroR
                     /note="color: #84b0dc; direction: RIGHT"
     CDS             4503..5114
                     /label=Translation 4503-5114
                     /note="color: #993366; direction: RIGHT"
     CDS             4515..5114
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4647..4666
                     /label=rv117
                     /note="color: #75c6a9"
     misc_feature    complement(5140..5177)
                     /label=rv023
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(5197..5218)
                     /label=rv059
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    5263..5285
                     /label=polAfwd
                     /note="color: #c6c9d1"
     polyA_site      5265..5422
                     /label=SV40 polyA
                     /note="color: #f8d3a9"
     polyA_signal    5283..5404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(5283..5300)
                     /label=EGFP-C-Rev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    5320..5341
                     /label=pCI-rev
                     /note="color: #f8d3a9"
     misc_feature    5374..5393
                     /label=EBV-rev
                     /note="color: #b4abac"
     misc_feature    5437..5471
                     /label=1L
                     /note="color: #b7e6d7"
     protein_bind    5438..5471
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(5505..5521)
                     /label=M13r
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(5505..5521)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    complement(5519..5538)
                     /label=LacOp-for
                     /note="color: #85dae9; direction: LEFT"
     protein_bind    5529..5545
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5553..5583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5553 .. 5559 / #ffffff / -10
                        2: 5560 .. 5577 / #ffffff
                        3: 5578 .. 5583 / #ffffff / -35"
     misc_feature    complement(5553..5572)
                     /label=pUCM13-rev-157
                     /note="color: #84b0dc; direction: LEFT"
     protein_bind    5598..5619
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     misc_feature    complement(5651..5671)
                     /label=ds1Lb
                     /note="color: #f8d3a9; direction: LEFT"
     rep_origin      complement(5907..6495)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6666..7526)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6666 .. 7457 / #ccffcc
                        2: 7458 .. 7526 / #ccffcc / signal sequence
                     Cleavage site after base 7457"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(6716..6735)
                     /label=AmpStop
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    7462..7481
                     /label=AmpStart
                     /note="color: #85dae9"
     promoter        complement(7527..7631)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7706..7724
                     /label=QE-for
                     /note="color: #d6b295"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtccata acttcgtata atgtatacta
      421 tacgaagtta tatcggacat tgattattga ctagttatta atagtaatca attacggggt
      481 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      541 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      601 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      661 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      721 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      781 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      841 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      901 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      961 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     1021 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     1081 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
     1141 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1201 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
     1261 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
     1321 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
     1381 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1441 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1501 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1561 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1621 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1681 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1741 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1801 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1861 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1921 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1981 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2041 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2101 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcattat
     2161 tgtttttcct cctttgccgc caccatggac tacaaagacg atgacgacaa ggatgaagag
     2221 tctggttctg tttctggtgg tgatgagctg tcagccatag gcgagcaggt gtttgcggtg
     2281 gagagcatcc ggaagaagcg cgtgcggaag ggcaaagttg aatatctggt gaagtggaaa
     2341 ggatggcccc ccaagtatag cacctgggag ccagaggagc acatcttgga ccctcgcctt
     2401 gtcatggcct acgaggagaa ggaggagaga gaccgagcct cggggtatag gaagagaggt
     2461 ccgaaaccca tcggtggcgg aggctctgga ggtggaggct ccgcaccccc caaaaagaaa
     2521 cgcaaggttg gataccggga tccaggttct gtttctggtg gtgatgagct gtcagccata
     2581 ggcgagcagg tgtttgcggt ggagagcatc cggaagaagc gcgtgcggaa gggcaaagtt
     2641 gaatatctgg tgaagtggaa aggatggccc cccaagtata gcacctggga gccagaggag
     2701 cacatcttgg accctcgcct tgtcatggcc tacgaggaga aggaggagag agaccgagcc
     2761 tcggggtata ggaagagagg tccgaaaccc atcggtggcg gaggctctgg aggtggaggc
     2821 tccgcacccc ccaaaaagaa acgcaaggtt ggataccggg atccacacgt gggtaggagc
     2881 ggttcgttcg gatcccatct tttttcggaa ttcggatccg gtggaggcgg gtctggaggc
     2941 gggggtagtg gcgggggtgg aagcgggggt ggaggcgggt cgggtggcgg aggtagcgga
     3001 ggcggtggaa gtggaggcgg tggaagtggt ggaggaggtt ctggtggcgg aggtagcggt
     3061 ggcggaggta gcggaggcgg tggaagtggt ggcggaggta gcggaggcgg tggatcggcg
     3121 cgccgtttca agaactaccc atacgatgtg cctgactatg ctggcaagtt gtctgaatca
     3181 gagattaggt ttggactgaa aacggaggtg atgggccaac atcttatcta tcacgatgtt
     3241 ttgtccagca cacaaaagac agcccatgaa ttggcaaata acaatgcccc cgagggtacc
     3301 ctcgtggttg cagataaaca gacagccggt aggggcggga tgagccgagt gtggcattct
     3361 caagaaggaa acggagtatg gatgagtttg atattgcgcc cggacattcc gctccaaaag
     3421 acgccccaac ttaccttgct ggcagctgta gcggtggttc agggaataga agaggccgcg
     3481 ggcatacaga ctgatataaa gtggccgaac gacatactca ttaacgggaa aaaaacagta
     3541 gggatactta ccgaaatgca agctgaggag gatcgagtgc gctccgtgat tattggtatt
     3601 ggcattaatg taaatcagca acccaatgat ttccccgatg agctcaagga cattgcgacg
     3661 agtctgtccc aggcagctgg ggaaaagatc gacagggcag gtgtaatcca gcatatcttg
     3721 ctttgtttcg aaaagcgata ccgagactac atgacccacg gtttcacgcc catcaagctc
     3781 ttgtgggagt catacgccct cggtattggc acgaacatga gggcgagaac tctcaacggg
     3841 acattttacg gtaaggcgtt gggtattgac gacgaagggg tacttttgtt ggaaactaat
     3901 gaaggcatta agaagatata cagcgcggac atcagcctta ggtgaaacga ggtagcggcc
     3961 gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat
     4021 tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc
     4081 ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag
     4141 cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc
     4201 ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac
     4261 ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca
     4321 aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt
     4381 gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa
     4441 aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata
     4501 atatggccac aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
     4561 tcccccgggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
     4621 ccgtcgaccc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
     4681 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
     4741 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
     4801 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
     4861 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
     4921 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
     4981 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
     5041 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
     5101 agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc gaccgaaagg agcgcagtgc
     5161 tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     5221 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     5281 ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     5341 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     5401 cttatcatgt ctgtataccg tggatccggc cggccccata acttcgtata gtatacatta
     5461 tacgaagtta tcccctgcag gcatgcaagc ttggcgtaat catggtcata gctgtttcct
     5521 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     5581 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     5641 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     5701 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5761 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5821 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5881 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5941 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     6001 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     6061 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     6121 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     6181 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     6241 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     6301 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     6361 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     6421 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     6481 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     6541 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6601 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6661 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6721 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6781 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6841 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6901 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6961 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     7021 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     7081 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     7141 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     7201 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     7261 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     7321 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     7381 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     7441 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     7501 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     7561 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7621 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     7681 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
