LOCUS       Exported                7822 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7822
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(29..47)
                     /label=GEX3-rev-25
                     /note="color: #d59687; direction: LEFT"
     misc_feature    complement(57..76)
                     /label=GEX3-rev
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    326..347
                     /label=rv060
                     /note="color: #75c6a9"
     misc_feature    327..346
                     /label=pUCM13-52
                     /note="color: #85dae9"
     misc_feature    358..379
                     /label=usL1b
                     /note="color: #84b0dc"
     misc_feature    359..375
                     /label=M13-40
                     /note="color: #c7b0e3"
     misc_feature    371..420
                     /label=rv141
                     /note="color: #b1ff67"
     misc_feature    complement(371..420)
                     /label=rv142
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    378..394
                     /label=M13
                     /note="color: #9eafd2"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    398..432
                     /label=L1
                     /note="color: #75c6a9"
     protein_bind    complement(398..431)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     promoter        434..2058
                     /label=CAG (not sequenced!)
                     /note="color: #75c6a9"
     misc_feature    434..815
                     /label=CMV enhancer
                     /note="color: #9eafd2"
     enhancer        436..815
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    complement(585..608)
                     /label=CAG 5' r
                     /note="color: #f58a5e; direction: LEFT"
     promoter        817..1092
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     TATA_signal     1066..1073
                     /label=TATAAA
                     /note="color: #75c6a9"
     TATA_signal     1080..1119
                     /label=replace with 2xTetO
                     /note="color: #ffef86"
     intron          1093..2101
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     TATA_signal     1095..1102
                     /label=transcript start
                     /note="color: #f58a5e"
     misc_feature    1981..2058
                     /label=homologousRegion
                     /note="color: #ff9ccd"
     misc_feature    2025..3088
                     /label=seq1
                     /note="color: #faac61"
     misc_feature    2025..2044
                     /label=rp022_CR002_fwd
                     /note="color: #f58a5e"
     misc_feature    2025..2044
                     /label=fwd better
                     /note="color: #85dae9"
     misc_feature    2059..2969
                     /label=newfragment
                     /note="color: #ff9ccd"
     misc_feature    2117..2135
                     /label=rv040
                     /note="color: #f58a5e"
     misc_feature    2176..2187
                     /label=Kosak_consensus
                     /note="color: #faac61"
     regulatory      2179..2188
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2185..3213
                     /label=Translation 2185-3213
                     /note="color: #993366; direction: RIGHT"
     CDS             2188..2211
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    2215..2244
                     /label=GibsonF
                     /note="color: #ff9ccd"
     misc_feature    2218..2244
                     /label=rv164_gibsonF_fwd
                     /note="color: #c7b0e3"
     misc_feature    2218..2237
                     /label=rv043
                     /note="color: #b7e6d7"
     misc_feature    2245..2520
                     /label=Taf3_PHD
                     /note="color: #d59687"
     misc_feature    complement(2499..2520)
                     /label=rv219
                     /note="color: #b7e6d7; direction: LEFT"
     misc_feature    2521..2610
                     /label=30mer linker
                     /note="color: #9eafd2"
     misc_feature    2521..2540
                     /label=fwd
                     /note="color: #f8d3a9"
     misc_feature    2599..2610
                     /label=rv185
                     /note="color: #f8d3a9"
     misc_feature    2611..2886
                     /label=Taf3_PHD
                     /note="color: #d59687"
     misc_feature    complement(2865..2886)
                     /label=rv219
                     /note="color: #b7e6d7; direction: LEFT"
     misc_feature    complement(2887..2913)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2887..2906
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2896..2913)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    2923..2949
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2926..2946
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    complement(2952..2989)
                     /label=GA rev pCR002
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(2952..2989)
                     /label=rp023_CR2_13XLrev
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(2952..2969)
                     /label=rev pCR002
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2962..2967
                     /label=intro site reader domain
                     /note="color: #75c6a9"
     misc_feature    2970..3004
                     /label=GA fwd, pv8 (rp012)
                     /note="color: #ff9ccd"
     misc_feature    complement(2970..2989)
                     /label=GA overhang
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    3010..3213
                     /label=13X-Linker
                     /note="color: #f58a5e"
     misc_feature    complement(3213..3261)
                     /label=rp007_Link_BASU
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3213..3231)
                     /label=rev primer
                     /label=rev primer; BioID1
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    3232..3259
                     /label=rp006_BASU_f
                     /note="color: #ffef86"
     misc_feature    3232..3258
                     /label=HA Tag
                     /note="color: #d59687"
     CDS             3232..3258
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     gene            3259..4038
                     /label=BASU*
                     /note="color: #c6c9d1; direction: RIGHT"
     CDS             3259..4038
                     /label=Translation 3259-4038
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3499..3504
                     /label=Split Site
                     /note="color: #75c6a9"
     misc_feature    complement(4019..4071)
                     /label=rp014_BASU_v8_r
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(4019..4071)
                     /label=GA rev, BASU (rp014)
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    complement(4019..4038)
                     /label=rev primer BASU
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    4030..4032
                     /label=E258S
                     /note="color: #f58a5e"
     misc_feature    4036..4038
                     /label=G2R
                     /note="color: #b1ff67"
     CDS             4039..4041
                     /label=Translation 4039-4041
                     /note="color: #993366; direction: RIGHT"
     misc_feature    4053..4078
                     /label=tb288
                     /note="color: #84b0dc"
     misc_feature    4058..4598
                     /label=IRES
                     /note="color: #ff9ccd"
     misc_feature    complement(4080..4098)
                     /label=IRES-rev
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    4136..4597
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    complement(4155..4173)
                     /label=rp019_IRESrev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    4322..4338
                     /label=rv022
                     /note="color: #75c6a9"
     CDS             4568..4601
                     /label=Translation 4568-4601
                     /note="color: #993366; direction: RIGHT"
     CDS             4599..5210
                     /label=puroR
                     /note="color: #84b0dc; direction: RIGHT"
     CDS             4599..5210
                     /label=Translation 4599-5210
                     /note="color: #993366; direction: RIGHT"
     CDS             4611..5210
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4743..4762
                     /label=rv117
                     /note="color: #75c6a9"
     misc_feature    complement(5236..5273)
                     /label=rv023
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(5293..5314)
                     /label=rv059
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    5359..5381
                     /label=polAfwd
                     /note="color: #c6c9d1"
     polyA_site      5361..5518
                     /label=SV40 polyA
                     /note="color: #f8d3a9"
     polyA_signal    5379..5500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(5379..5396)
                     /label=EGFP-C-Rev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    5416..5437
                     /label=pCI-rev
                     /note="color: #f8d3a9"
     misc_feature    5470..5489
                     /label=EBV-rev
                     /note="color: #b4abac"
     misc_feature    5533..5567
                     /label=1L
                     /note="color: #b7e6d7"
     protein_bind    5534..5567
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(5601..5617)
                     /label=M13r
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(5601..5617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    complement(5615..5634)
                     /label=LacOp-for
                     /note="color: #85dae9; direction: LEFT"
     protein_bind    5625..5641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5649..5679)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5649 .. 5655 / #ffffff / -10
                        2: 5656 .. 5673 / #ffffff
                        3: 5674 .. 5679 / #ffffff / -35"
     misc_feature    complement(5649..5668)
                     /label=pUCM13-rev-157
                     /note="color: #84b0dc; direction: LEFT"
     protein_bind    5694..5715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     misc_feature    complement(5747..5767)
                     /label=ds1Lb
                     /note="color: #f8d3a9; direction: LEFT"
     rep_origin      complement(6003..6591)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6762..7622)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6762 .. 7553 / #ccffcc
                        2: 7554 .. 7622 / #ccffcc / signal sequence
                     Cleavage site after base 7553"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(6812..6831)
                     /label=AmpStop
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    7558..7577
                     /label=AmpStart
                     /note="color: #85dae9"
     promoter        complement(7623..7727)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7802..7820
                     /label=QE-for
                     /note="color: #d6b295"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtccata acttcgtata atgtatacta
      421 tacgaagtta tatcggacat tgattattga ctagttatta atagtaatca attacggggt
      481 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      541 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      601 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      661 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      721 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      781 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      841 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      901 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      961 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     1021 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     1081 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
     1141 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1201 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
     1261 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
     1321 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
     1381 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1441 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1501 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1561 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1621 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1681 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1741 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1801 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1861 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1921 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1981 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2041 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2101 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcattat
     2161 tgtttttcct cctttgccgc caccatggac tacaaagacg atgacgacaa ggatgaagag
     2221 tctggttctg tttctggtgg tgatagtgcc aaagcccccg tgaggagcgt cgtgactgag
     2281 acggtcagca cttatgtgat ccgtgatgag tggggcaatc agatctggat ctgtccaggg
     2341 tgcaacaagc ctgatgacgg gagcccgatg attggctgtg acgactgtga cgattggtac
     2401 cactggccct gtgtgggaat catggctgct cctccagaag agatgcagtg gttctgcccg
     2461 aagtgtgcca acaagataaa gaaagacaag aagcacaaga agaggaagca ccgtgcgcac
     2521 ggtggtggcg gatcagaagg aggcggtagc gggggccctg gttcgggagg ggaaggttct
     2581 gctgggggag ggagcgctgg cggggggtct agtgccaaag cccccgtgag gagcgtcgtg
     2641 actgagacgg tcagcactta tgtgatccgt gatgagtggg gcaatcagat ctggatctgt
     2701 ccagggtgca acaagcctga tgacgggagc ccgatgattg gctgtgacga ctgtgacgat
     2761 tggtaccact ggccctgtgt gggaatcatg gctgctcctc cagaagagat gcagtggttc
     2821 tgcccgaagt gtgccaacaa gataaagaaa gacaagaagc acaagaagag gaagcaccgt
     2881 gcgcacatcg gtggcggagg ctctggaggt ggaggctccg caccccccaa aaagaaacgc
     2941 aaggttggat accgggatcc acacgtgggt aggagcggtt cgttcggatc ccatcttttt
     3001 tcggaattcg gatccggtgg aggcgggtct ggaggcgggg gtagtggcgg gggtggaagc
     3061 gggggtggag gcgggtcggg tggcggaggt agcggaggcg gtggaagtgg aggcggtgga
     3121 agtggtggag gaggttctgg tggcggaggt agcggtggcg gaggtagcgg aggcggtgga
     3181 agtggtggcg gaggtagcgg aggcggtgga tcggcgcgcc gtttcaagaa ctacccatac
     3241 gatgtgcctg actatgctgg caagttgtct gaatcagaga ttaggtttgg actgaaaacg
     3301 gaggtgatgg gccaacatct tatctatcac gatgttttgt ccagcacaca aaagacagcc
     3361 catgaattgg caaataacaa tgcccccgag ggtaccctcg tggttgcaga taaacagaca
     3421 gccggtaggg gcgggatgag ccgagtgtgg cattctcaag aaggaaacgg agtatggatg
     3481 agtttgatat tgcgcccgga cattccgctc caaaagacgc cccaacttac cttgctggca
     3541 gctgtagcgg tggttcaggg aatagaagag gccgcgggca tacagactga tataaagtgg
     3601 ccgaacgaca tactcattaa cgggaaaaaa acagtaggga tacttaccga aatgcaagct
     3661 gaggaggatc gagtgcgctc cgtgattatt ggtattggca ttaatgtaaa tcagcaaccc
     3721 aatgatttcc ccgatgagct caaggacatt gcgacgagtc tgtcccaggc agctggggaa
     3781 aagatcgaca gggcaggtgt aatccagcat atcttgcttt gtttcgaaaa gcgataccga
     3841 gactacatga cccacggttt cacgcccatc aagctcttgt gggagtcata cgccctcggt
     3901 attggcacga acatgagggc gagaactctc aacgggacat tttacggtaa ggcgttgggt
     3961 attgacgacg aaggggtact tttgttggaa actaatgaag gcattaagaa gatatacagc
     4021 gcggacatca gccttaggtg aaacgaggta gcggccgccg aagccgcttg gaataaggcc
     4081 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4141 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4201 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4261 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4321 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4381 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4441 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4501 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa cgtctaggcc ccccgaacca
     4561 cggggacgtg gttttccttt gaaaaacacg atgataatat ggccacaacc atgaccgagt
     4621 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc ccgggccgta cgcaccctcg
     4681 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgacccggac cgccacatcg
     4741 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     4801 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     4861 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     4921 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     4981 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     5041 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     5101 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     5161 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     5221 acgacccgca gcgcccgacc gaaaggagcg cagtgctacg agatttcgat tccaccgccg
     5281 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
     5341 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
     5401 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5461 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt ataccgtgga
     5521 tccggccggc cccataactt cgtatagtat acattatacg aagttatccc ctgcaggcat
     5581 gcaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     5641 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     5701 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     5761 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     5821 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     5881 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     5941 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6001 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6061 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     6121 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     6181 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     6241 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     6301 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     6361 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     6421 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     6481 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     6541 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     6601 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     6661 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     6721 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     6781 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     6841 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     6901 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     6961 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7021 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7081 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     7141 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     7201 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     7261 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     7321 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     7381 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     7441 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     7501 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     7561 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     7621 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     7681 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     7741 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     7801 cgtatcacga ggccctttcg tc
//
