LOCUS       Exported                7768 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7768
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(29..47)
                     /label=GEX3-rev-25
                     /note="color: #d59687; direction: LEFT"
     misc_feature    complement(57..76)
                     /label=GEX3-rev
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    326..347
                     /label=rv060
                     /note="color: #75c6a9"
     misc_feature    327..346
                     /label=pUCM13-52
                     /note="color: #85dae9"
     misc_feature    358..379
                     /label=usL1b
                     /note="color: #84b0dc"
     misc_feature    359..375
                     /label=M13-40
                     /note="color: #c7b0e3"
     misc_feature    371..420
                     /label=rv141
                     /note="color: #b1ff67"
     misc_feature    complement(371..420)
                     /label=rv142
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    378..394
                     /label=M13
                     /note="color: #9eafd2"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    398..432
                     /label=L1
                     /note="color: #75c6a9"
     protein_bind    complement(398..431)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     promoter        434..2058
                     /label=CAG (not sequenced!)
                     /note="color: #75c6a9"
     misc_feature    434..815
                     /label=CMV enhancer
                     /note="color: #9eafd2"
     enhancer        436..815
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    complement(585..608)
                     /label=CAG 5' r
                     /note="color: #f58a5e; direction: LEFT"
     promoter        817..1092
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     TATA_signal     1066..1073
                     /label=TATAAA
                     /note="color: #75c6a9"
     TATA_signal     1080..1119
                     /label=replace with 2xTetO
                     /note="color: #ffef86"
     intron          1093..2101
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     TATA_signal     1095..1102
                     /label=transcript start
                     /note="color: #f58a5e"
     misc_feature    1981..2058
                     /label=homologousRegion
                     /note="color: #ff9ccd"
     misc_feature    2025..3034
                     /label=seq1
                     /note="color: #faac61"
     misc_feature    2025..2044
                     /label=rp022_CR002_fwd
                     /note="color: #f58a5e"
     misc_feature    2025..2044
                     /label=fwd better
                     /note="color: #85dae9"
     misc_feature    2117..2135
                     /label=rv040
                     /note="color: #f58a5e"
     misc_feature    2176..2187
                     /label=Kosak_consensus
                     /note="color: #faac61"
     regulatory      2179..2188
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2185..3984
                     /label=Translation 2185-3984
                     /note="color: #993366; direction: RIGHT"
     CDS             2188..2211
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    2215..2244
                     /label=GibsonF
                     /note="color: #ff9ccd"
     misc_feature    2218..2244
                     /label=rv164_gibsonF_fwd
                     /note="color: #c7b0e3"
     misc_feature    2218..2237
                     /label=rv043
                     /note="color: #b7e6d7"
     misc_feature    2225..2283
                     /label=rv131_Cbx7(CD)_fwd
                     /note="color: #75c6a9"
     misc_feature    2245..2469
                     /label=Cbx7_Chromo
                     /note="color: #9eafd2"
     misc_feature    complement(2434..2489)
                     /label=rv132_Cbx7(CD)_rev
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(2470..2499)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2470..2489
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2479..2496)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(2488..2505)
                     /label=rev
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    2506..2532
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2509..2529
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    2520..2561
                     /label=rv185_Taf3_GAfwd
                     /note="color: #c7b0e3"
     misc_feature    2545..2832
                     /label=Taf3_PHD
                     /note="color: #f8d3a9"
     misc_feature    complement(2833..2859)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2833..2852
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2842..2859)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    2869..2895
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2872..2892
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    complement(2898..2935)
                     /label=GA rev pCR002
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(2898..2935)
                     /label=rp023_CR2_13XLrev
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(2898..2915)
                     /label=rev pCR002
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2908..2913
                     /label=intro site reader domain
                     /note="color: #75c6a9"
     misc_feature    2916..2950
                     /label=GA fwd, pv8 (rp012)
                     /note="color: #ff9ccd"
     misc_feature    complement(2916..2935)
                     /label=GA overhang
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2956..3159
                     /label=13X-Linker
                     /note="color: #f58a5e"
     misc_feature    complement(3159..3207)
                     /label=rp007_Link_BASU
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3159..3177)
                     /label=rev primer
                     /label=rev primer; BioID1
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    3178..3205
                     /label=rp006_BASU_f
                     /note="color: #ffef86"
     misc_feature    3178..3204
                     /label=HA Tag
                     /note="color: #d59687"
     CDS             3178..3204
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     gene            3205..3984
                     /label=BASU*
                     /note="color: #c6c9d1; direction: RIGHT"
     misc_feature    3445..3450
                     /label=Split Site
                     /note="color: #75c6a9"
     misc_feature    complement(3965..4017)
                     /label=rp014_BASU_v8_r
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3965..4017)
                     /label=GA rev, BASU (rp014)
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    complement(3965..3984)
                     /label=rev primer BASU
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    3976..3978
                     /label=E258S
                     /note="color: #f58a5e"
     misc_feature    3982..3984
                     /label=G2R
                     /note="color: #b1ff67"
     CDS             3985..3987
                     /label=Translation 3985-3987
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3999..4024
                     /label=tb288
                     /note="color: #84b0dc"
     misc_feature    4004..4544
                     /label=IRES
                     /note="color: #ff9ccd"
     misc_feature    complement(4026..4044)
                     /label=IRES-rev
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    4082..4543
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    complement(4101..4119)
                     /label=rp019_IRESrev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    4268..4284
                     /label=rv022
                     /note="color: #75c6a9"
     CDS             4514..4547
                     /label=Translation 4514-4547
                     /note="color: #993366; direction: RIGHT"
     CDS             4545..5156
                     /label=puroR
                     /note="color: #84b0dc; direction: RIGHT"
     CDS             4545..5156
                     /label=Translation 4545-5156
                     /note="color: #993366; direction: RIGHT"
     CDS             4557..5156
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4689..4708
                     /label=rv117
                     /note="color: #75c6a9"
     misc_feature    complement(5182..5219)
                     /label=rv023
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(5239..5260)
                     /label=rv059
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    5305..5327
                     /label=polAfwd
                     /note="color: #c6c9d1"
     polyA_site      5307..5464
                     /label=SV40 polyA
                     /note="color: #f8d3a9"
     polyA_signal    5325..5446
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(5325..5342)
                     /label=EGFP-C-Rev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    5362..5383
                     /label=pCI-rev
                     /note="color: #f8d3a9"
     misc_feature    5416..5435
                     /label=EBV-rev
                     /note="color: #b4abac"
     misc_feature    5479..5513
                     /label=1L
                     /note="color: #b7e6d7"
     protein_bind    5480..5513
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(5547..5563)
                     /label=M13r
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(5547..5563)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    complement(5561..5580)
                     /label=LacOp-for
                     /note="color: #85dae9; direction: LEFT"
     protein_bind    5571..5587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5595..5625)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5595 .. 5601 / #ffffff / -10
                        2: 5602 .. 5619 / #ffffff
                        3: 5620 .. 5625 / #ffffff / -35"
     misc_feature    complement(5595..5614)
                     /label=pUCM13-rev-157
                     /note="color: #84b0dc; direction: LEFT"
     protein_bind    5640..5661
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     misc_feature    complement(5693..5713)
                     /label=ds1Lb
                     /note="color: #f8d3a9; direction: LEFT"
     rep_origin      complement(5949..6537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6708..7568)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6708 .. 7499 / #ccffcc
                        2: 7500 .. 7568 / #ccffcc / signal sequence
                     Cleavage site after base 7499"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(6758..6777)
                     /label=AmpStop
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    7504..7523
                     /label=AmpStart
                     /note="color: #85dae9"
     promoter        complement(7569..7673)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7748..7766
                     /label=QE-for
                     /note="color: #d6b295"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtccata acttcgtata atgtatacta
      421 tacgaagtta tatcggacat tgattattga ctagttatta atagtaatca attacggggt
      481 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      541 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      601 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      661 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      721 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      781 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      841 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      901 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      961 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     1021 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     1081 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
     1141 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1201 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
     1261 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
     1321 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
     1381 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1441 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1501 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1561 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1621 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1681 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1741 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1801 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1861 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1921 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1981 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2041 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2101 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcattat
     2161 tgtttttcct cctttgccgc caccatggac tacaaagacg atgacgacaa ggatgaagag
     2221 tctggttctg tttctggtgg tgatgagctg tcagccatag gcgagcaggt gtttgcggtg
     2281 gagagcatcc ggaagaagcg cgtgcggaag ggcaaagttg aatatctggt gaagtggaaa
     2341 ggatggcccc ccaagtatag cacctgggag ccagaggagc acatcttgga ccctcgcctt
     2401 gtcatggcct acgaggagaa ggaggagaga gaccgagcct cggggtatag gaagagaggt
     2461 ccgaaaccca tcggtggcgg aggctctgga ggtggaggct ccgcaccccc caaaaagaaa
     2521 cgcaaggttg gataccggga tccaggcggg gggtctagtg caaaggcacc tgtccgaagt
     2581 gtcgtcacag aaactgtgtc tacttatgtc atcagagacg aatggggaaa tcagatttgg
     2641 atctgtcccg gctgcaataa gccagatgac ggatcaccta tgataggatg tgacgactgc
     2701 gatgactggt atcactggcc ttgcgttggg atcatggccg ccccccccga agagatgcaa
     2761 tggttttgcc ccaaatgcgc caataaaata aaaaaggaca agaaacataa gaaacgcaag
     2821 catcgcgctc acatcggtgg cggaggctct ggaggtggag gctccgcacc ccccaaaaag
     2881 aaacgcaagg ttggataccg ggatccacac gtgggtagga gcggttcgtt cggatcccat
     2941 cttttttcgg aattcggatc cggtggaggc gggtctggag gcgggggtag tggcgggggt
     3001 ggaagcgggg gtggaggcgg gtcgggtggc ggaggtagcg gaggcggtgg aagtggaggc
     3061 ggtggaagtg gtggaggagg ttctggtggc ggaggtagcg gtggcggagg tagcggaggc
     3121 ggtggaagtg gtggcggagg tagcggaggc ggtggatcgg cgcgccgttt caagaactac
     3181 ccatacgatg tgcctgacta tgctggcaag ttgtctgaat cagagattag gtttggactg
     3241 aaaacggagg tgatgggcca acatcttatc tatcacgatg ttttgtccag cacacaaaag
     3301 acagcccatg aattggcaaa taacaatgcc cccgagggta ccctcgtggt tgcagataaa
     3361 cagacagccg gtaggggcgg gatgagccga gtgtggcatt ctcaagaagg aaacggagta
     3421 tggatgagtt tgatattgcg cccggacatt ccgctccaaa agacgcccca acttaccttg
     3481 ctggcagctg tagcggtggt tcagggaata gaagaggccg cgggcataca gactgatata
     3541 aagtggccga acgacatact cattaacggg aaaaaaacag tagggatact taccgaaatg
     3601 caagctgagg aggatcgagt gcgctccgtg attattggta ttggcattaa tgtaaatcag
     3661 caacccaatg atttccccga tgagctcaag gacattgcga cgagtctgtc ccaggcagct
     3721 ggggaaaaga tcgacagggc aggtgtaatc cagcatatct tgctttgttt cgaaaagcga
     3781 taccgagact acatgaccca cggtttcacg cccatcaagc tcttgtggga gtcatacgcc
     3841 ctcggtattg gcacgaacat gagggcgaga actctcaacg ggacatttta cggtaaggcg
     3901 ttgggtattg acgacgaagg ggtacttttg ttggaaacta atgaaggcat taagaagata
     3961 tacagcgcgg acatcagcct taggtgaaac gaggtagcgg ccgccgaagc cgcttggaat
     4021 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     4081 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     4141 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     4201 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     4261 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     4321 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     4381 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     4441 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaacgtc taggcccccc
     4501 gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatga
     4561 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtcccccgg gccgtacgca
     4621 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgac ccggaccgcc
     4681 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     4741 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     4801 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     4861 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     4921 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     4981 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     5041 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     5101 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     5161 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcagt gctacgagat ttcgattcca
     5221 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
     5281 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
     5341 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     5401 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
     5461 cgtggatccg gccggcccca taacttcgta tagtatacat tatacgaagt tatcccctgc
     5521 aggcatgcaa gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5581 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5641 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5701 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5761 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5821 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5881 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5941 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6001 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6061 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6121 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6181 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6241 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6301 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6361 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag
     6421 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6481 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6541 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6601 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6661 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6721 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6781 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6841 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6901 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6961 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7021 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7081 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7141 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7201 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7261 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7321 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7381 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7441 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7501 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7561 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7621 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7681 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     7741 aataggcgta tcacgaggcc ctttcgtc
//
