LOCUS       Exported                7393 bp DNA     circular SYN 29-NOV-2021
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 29, 2021 from SnapGene Viewer 5.3.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7393
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    complement(29..47)
                     /label=GEX3-rev-25
                     /note="color: #d59687; direction: LEFT"
     misc_feature    complement(57..76)
                     /label=GEX3-rev
                     /note="color: #d6b295; direction: LEFT"
     misc_feature    326..347
                     /label=rv060
                     /note="color: #75c6a9"
     misc_feature    327..346
                     /label=pUCM13-52
                     /note="color: #85dae9"
     misc_feature    358..379
                     /label=usL1b
                     /note="color: #84b0dc"
     misc_feature    359..375
                     /label=M13-40
                     /note="color: #c7b0e3"
     misc_feature    371..420
                     /label=rv141
                     /note="color: #b1ff67"
     misc_feature    complement(371..420)
                     /label=rv142
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    378..394
                     /label=M13
                     /note="color: #9eafd2"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     misc_feature    398..432
                     /label=L1
                     /note="color: #75c6a9"
     protein_bind    complement(398..431)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: LEFT"
     promoter        434..2058
                     /label=CAG (not sequenced!)
                     /note="color: #75c6a9"
     misc_feature    434..815
                     /label=CMV enhancer
                     /note="color: #9eafd2"
     enhancer        436..815
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     misc_feature    complement(585..608)
                     /label=CAG 5' r
                     /note="color: #f58a5e; direction: LEFT"
     promoter        817..1092
                     /label=chicken beta-actin promoter
                     /note="color: #ffffff; direction: RIGHT"
     TATA_signal     1066..1073
                     /label=TATAAA
                     /note="color: #75c6a9"
     TATA_signal     1080..1119
                     /label=replace with 2xTetO
                     /note="color: #ffef86"
     intron          1093..2101
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
                     /note="color: #------"
     TATA_signal     1095..1102
                     /label=transcript start
                     /note="color: #f58a5e"
     misc_feature    1981..2058
                     /label=homologousRegion
                     /note="color: #ff9ccd"
     misc_feature    2025..2659
                     /label=seq1
                     /note="color: #faac61"
     misc_feature    2025..2044
                     /label=rp022_CR002_fwd
                     /note="color: #f58a5e"
     misc_feature    2025..2044
                     /label=fwd better
                     /note="color: #85dae9"
     misc_feature    2117..2135
                     /label=rv040
                     /note="color: #f58a5e"
     misc_feature    2176..2187
                     /label=Kosak_consensus
                     /note="color: #faac61"
     regulatory      2179..2188
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
     CDS             2185..2784
                     /label=Translation 2185-2784
                     /note="color: #993366; direction: RIGHT"
     CDS             2188..2211
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /note="color: #cc99b2"
                     /translation="DYKDDDDK"
     misc_feature    2215..2244
                     /label=GibsonF
                     /note="color: #ff9ccd"
     misc_feature    2218..2244
                     /label=rv164_gibsonF_fwd
                     /note="color: #c7b0e3"
     misc_feature    2218..2237
                     /label=rv043
                     /note="color: #b7e6d7"
     misc_feature    2245..2457
                     /label=Mbd1_MBD
                     /note="color: #c6c9d1"
     misc_feature    2305..2307
                     /label=R22A
                     /note="color: #75c6a9"
     misc_feature    complement(2458..2484)
                     /label=GibsonR
                     /note="color: #c6c9d1; direction: LEFT"
     misc_feature    2458..2477
                     /label=rv_229_gibsonR_fwd
                     /note="color: #c7b0e3"
     misc_feature    complement(2467..2484)
                     /label=rv044
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    2494..2520
                     /label=nls
                     /note="color: #ff9ccd"
     CDS             2497..2517
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     misc_feature    complement(2523..2560)
                     /label=rp023_CR2_13XLrev
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(2523..2560)
                     /label=GA rev pCR002
                     /note="color: #ffef86; direction: LEFT"
     misc_feature    complement(2523..2540)
                     /label=rev pCR002
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2524..2580
                     /label=ce001_Chromo/Link
                     /note="color: #f58a5e"
     misc_feature    2533..2538
                     /label=intro site reader domain
                     /note="color: #75c6a9"
     misc_feature    2541..2575
                     /label=GA fwd, pv8 (rp012)
                     /note="color: #ff9ccd"
     misc_feature    complement(2541..2560)
                     /label=GA overhang
                     /note="color: #b4abac; direction: LEFT"
     misc_feature    2554..2580
                     /label=ce001_bs
                     /note="color: #ffef86"
     misc_feature    2581..2784
                     /label=13X-Linker
                     /note="color: #f58a5e"
     misc_feature    complement(2784..2832)
                     /label=rp007_Link_BASU
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(2784..2802)
                     /label=rev primer
                     /label=rev primer; BioID1
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    2803..2830
                     /label=rp006_BASU_f
                     /note="color: #ffef86"
     misc_feature    2803..2829
                     /label=HA Tag
                     /note="color: #d59687"
     CDS             2803..2829
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     gene            2830..3609
                     /label=BASU*
                     /note="color: #c6c9d1; direction: RIGHT"
     CDS             2830..3609
                     /label=Translation 2830-3609
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3070..3075
                     /label=Split Site
                     /note="color: #75c6a9"
     misc_feature    complement(3590..3642)
                     /label=GA rev, BASU (rp014)
                     /note="color: #ff9ccd; direction: LEFT"
     misc_feature    complement(3590..3642)
                     /label=rp014_BASU_v8_r
                     /note="color: #faac61; direction: LEFT"
     misc_feature    complement(3590..3609)
                     /label=rev primer BASU
                     /note="color: #85dae9; direction: LEFT"
     misc_feature    3601..3603
                     /label=E258S
                     /note="color: #f58a5e"
     misc_feature    3607..3609
                     /label=G2R
                     /note="color: #b1ff67"
     CDS             3610..3612
                     /label=Translation 3610-3612
                     /note="color: #993366; direction: RIGHT"
     misc_feature    3624..3649
                     /label=tb288
                     /note="color: #84b0dc"
     misc_feature    3629..4169
                     /label=IRES
                     /note="color: #ff9ccd"
     misc_feature    complement(3651..3669)
                     /label=IRES-rev
                     /note="color: #b1ff67; direction: LEFT"
     misc_feature    3707..4168
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="color: #a6acb3"
     misc_feature    complement(3726..3744)
                     /label=rp019_IRESrev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    3893..3909
                     /label=rv022
                     /note="color: #75c6a9"
     CDS             4139..4172
                     /label=Translation 4139-4172
                     /note="color: #993366; direction: RIGHT"
     CDS             4170..4781
                     /label=puroR
                     /note="color: #84b0dc; direction: RIGHT"
     CDS             4170..4781
                     /label=Translation 4170-4781
                     /note="color: #993366; direction: RIGHT"
     CDS             4182..4781
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    4314..4333
                     /label=rv117
                     /note="color: #75c6a9"
     misc_feature    complement(4807..4844)
                     /label=rv023
                     /note="color: #75c6a9; direction: LEFT"
     misc_feature    complement(4864..4885)
                     /label=rv059
                     /note="color: #f58a5e; direction: LEFT"
     misc_feature    4930..4952
                     /label=polAfwd
                     /note="color: #c6c9d1"
     polyA_site      4932..5089
                     /label=SV40 polyA
                     /note="color: #f8d3a9"
     polyA_signal    4950..5071
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     misc_feature    complement(4950..4967)
                     /label=EGFP-C-Rev
                     /note="color: #f8d3a9; direction: LEFT"
     misc_feature    4987..5008
                     /label=pCI-rev
                     /note="color: #f8d3a9"
     misc_feature    5041..5060
                     /label=EBV-rev
                     /note="color: #b4abac"
     misc_feature    5104..5138
                     /label=1L
                     /note="color: #b7e6d7"
     protein_bind    5105..5138
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
                     /note="color: #31849b; direction: RIGHT"
     misc_feature    complement(5172..5188)
                     /label=M13r
                     /note="color: #ff9ccd; direction: LEFT"
     primer_bind     complement(5172..5188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     misc_feature    complement(5186..5205)
                     /label=LacOp-for
                     /note="color: #85dae9; direction: LEFT"
     protein_bind    5196..5212
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(5220..5250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 5220 .. 5226 / #ffffff / -10
                        2: 5227 .. 5244 / #ffffff
                        3: 5245 .. 5250 / #ffffff / -35"
     misc_feature    complement(5220..5239)
                     /label=pUCM13-rev-157
                     /note="color: #84b0dc; direction: LEFT"
     protein_bind    5265..5286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     misc_feature    complement(5318..5338)
                     /label=ds1Lb
                     /note="color: #f8d3a9; direction: LEFT"
     rep_origin      complement(5574..6162)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(6333..7193)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 6333 .. 7124 / #ccffcc
                        2: 7125 .. 7193 / #ccffcc / signal sequence
                     Cleavage site after base 7124"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     misc_feature    complement(6383..6402)
                     /label=AmpStop
                     /note="color: #84b0dc; direction: LEFT"
     misc_feature    7129..7148
                     /label=AmpStart
                     /note="color: #85dae9"
     promoter        complement(7194..7298)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     misc_feature    7373..7391
                     /label=QE-for
                     /note="color: #d6b295"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtccata acttcgtata atgtatacta
      421 tacgaagtta tatcggacat tgattattga ctagttatta atagtaatca attacggggt
      481 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
      541 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
      601 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
      661 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
      721 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
      781 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
      841 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      901 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      961 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
     1021 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
     1081 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
     1141 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
     1201 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
     1261 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
     1321 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
     1381 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1441 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1501 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1561 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1621 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1681 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1741 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1801 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1861 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1921 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1981 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     2041 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     2101 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcattat
     2161 tgtttttcct cctttgccgc caccatggac tacaaagacg atgacgacaa ggatgaagag
     2221 tctggttctg tttctggtgg tgatgctgag tcctggcagg actgcccagc cctgggccct
     2281 ggctggaaac gccgagagtc ctttgcaaag tcaggggcct cgtttggacg ctcagacatc
     2341 tattaccaga gccccacagg agaaaagatc cgaagcaaag ttgagctgac tcggtacttg
     2401 ggccctgcat gtgatcttac cctgtttgac ttcagacaag gcaccttatg ccatcccatc
     2461 ggtggcggag gctctggagg tggaggctcc gcacccccca aaaagaaacg caaggttgga
     2521 taccgggatc cacacgtggg taggagcggt tcgttcggat cccatctttt ttcggaattc
     2581 ggatccggtg gaggcgggtc tggaggcggg ggtagtggcg ggggtggaag cgggggtgga
     2641 ggcgggtcgg gtggcggagg tagcggaggc ggtggaagtg gaggcggtgg aagtggtgga
     2701 ggaggttctg gtggcggagg tagcggtggc ggaggtagcg gaggcggtgg aagtggtggc
     2761 ggaggtagcg gaggcggtgg atcggcgcgc cgtttcaaga actacccata cgatgtgcct
     2821 gactatgctg gcaagttgtc tgaatcagag attaggtttg gactgaaaac ggaggtgatg
     2881 ggccaacatc ttatctatca cgatgttttg tccagcacac aaaagacagc ccatgaattg
     2941 gcaaataaca atgcccccga gggtaccctc gtggttgcag ataaacagac agccggtagg
     3001 ggcgggatga gccgagtgtg gcattctcaa gaaggaaacg gagtatggat gagtttgata
     3061 ttgcgcccgg acattccgct ccaaaagacg ccccaactta ccttgctggc agctgtagcg
     3121 gtggttcagg gaatagaaga ggccgcgggc atacagactg atataaagtg gccgaacgac
     3181 atactcatta acgggaaaaa aacagtaggg atacttaccg aaatgcaagc tgaggaggat
     3241 cgagtgcgct ccgtgattat tggtattggc attaatgtaa atcagcaacc caatgatttc
     3301 cccgatgagc tcaaggacat tgcgacgagt ctgtcccagg cagctgggga aaagatcgac
     3361 agggcaggtg taatccagca tatcttgctt tgtttcgaaa agcgataccg agactacatg
     3421 acccacggtt tcacgcccat caagctcttg tgggagtcat acgccctcgg tattggcacg
     3481 aacatgaggg cgagaactct caacgggaca ttttacggta aggcgttggg tattgacgac
     3541 gaaggggtac ttttgttgga aactaatgaa ggcattaaga agatatacag cgcggacatc
     3601 agccttaggt gaaacgaggt agcggccgcc gaagccgctt ggaataaggc cggtgtgcgt
     3661 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     3721 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     3781 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     3841 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     3901 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     3961 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     4021 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     4081 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     4141 ggttttcctt tgaaaaacac gatgataata tggccacaac catgaccgag tacaagccca
     4201 cggtgcgcct cgccacccgc gacgacgtcc cccgggccgt acgcaccctc gccgccgcgt
     4261 tcgccgacta ccccgccacg cgccacaccg tcgacccgga ccgccacatc gagcgggtca
     4321 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     4381 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     4441 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     4501 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     4561 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     4621 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     4681 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     4741 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc cacgacccgc
     4801 agcgcccgac cgaaaggagc gcagtgctac gagatttcga ttccaccgcc gccttctatg
     4861 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     4921 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     4981 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     5041 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtgg atccggccgg
     5101 ccccataact tcgtatagta tacattatac gaagttatcc cctgcaggca tgcaagcttg
     5161 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5221 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5281 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     5341 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     5401 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     5461 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     5521 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     5581 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     5641 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     5701 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     5761 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     5821 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     5881 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     5941 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     6001 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     6061 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     6121 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     6181 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     6241 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     6301 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     6361 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     6421 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     6481 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     6541 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     6601 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     6661 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     6721 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     6781 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     6841 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     6901 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     6961 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     7021 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     7081 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     7141 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     7201 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     7261 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     7321 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     7381 aggccctttc gtc
//
