LOCUS       pMH010_phiC31_neo-5xTet 8508 bp ds-DNA     circular     18-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      59..741
                     /label="Ori"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          810..842
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          1047..1079
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    1287..1559
                     /label="phiC31attB"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    1617..1622
                     /label="insertion found from sequencing"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    1637..2437
                     /label="NeoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1712..1733
                     /label="MH023"
                     /note="sequence: gactgggcacaacagacaatcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(1959..1985)
                     /label="SL009- Sequencing from Neo (check for phiC31)"
                     /note="sequence: atactttctcggcaggagcaaggtgag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2305..2331
                     /label="SL010- Sequencing from Neo fwd (TRE check)"
                     /note="sequence: catagcgttggctacccgtgatattgc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2332..2353
                     /label="MH035"
                     /note="sequence: tgaagagcttggcggcgaatgg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2454..2730
                     /label="BGHpA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2690..2711
                     /label="MH025 binding"
                     /note="sequence: gtgggctctatggcttctgagg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2735..2774
                     /label="MH003"
                     /note="sequence: GCAAAGCCTAGGAAGACGTacgTATACGCGTCGAGTTTAC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(2735..2774)
                     /label="MH004"
                     /note="sequence: GTAAACTCGACGCGTATAcgtACGTCTTCCTAGGCTTTGC"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    2751..2756
                     /label="Added Cutsite BsiWI"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2766..2967
                     /label="TRE-tight"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2775..2793
                     /label="tetO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2811..2829
                     /label="tetO"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2847..2865
                     /label="tetO"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2882..2900
                     /label="tetO"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2918..2936
                     /label="tetO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3023..3028
                     /label="Kozak"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    3029..3406
                     /label="H2B"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3178..3199)
                     /label="MH036"
                     /note="sequence: ggacgaaatgccggtgtcaggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          3431..3448
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3431..4147
                     /label="citrine"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          4028..4049
                     /label="MH013"
                     /note="sequence: cactacctgagctaccagtccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          4180..4207
                     /label="SL020-Lambda Gibson seq fwd"
                     /note="sequence: gtaagtgtacccaattcgccctatagtg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          4197..4218
                     /label="T7\Primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    4230..4613
                     /label="SV40\pA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          4394..4426
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          4584..4631
                     /label="SL031-H2B mCherry Gibson fwd"
                     /note="sequence: ggtttgtccaaactcatcaatgtatcttaacgcgtaaattgtaagcgt"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          4598..4657
                     /label="MH031"
                     /note="sequence: catcaatgtatcttaacgcgtaaattgtaagcgtctcatgaagacgacgcgaaattcagc"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          4614..4631
                     /label="SL031-H2B mCherry Gibson fwd"
                     /note="sequence: cgcgtaaattgtaagcgt"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          4636..4657
                     /label="MH031 binding"
                     /note="sequence: tgaagacgacgcgaaattcagc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    4636..5904
                     /label="1.2kb Lambda"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     gene            complement(4692..5240)
                     /label="ea22 gene"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     gene            complement(5684..5869)
                     /label="orf61 gene"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(5883..5904)
                     /label="MH032 binding"
                     /note="sequence: aaagctggaaatgatggtggcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(5883..5942)
                     /label="MH032"
                     /note="sequence: GTATTGCATAAGACTACATTcatatggacgtccctaggaaagctggaaatgatggtggcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    5905..5922
                     /label="inserted MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    5923..6185
                     /label="RSV Promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6152..6173)
                     /label="MH014"
                     /note="sequence: TGTGGTGAATGGTCAAATGGCG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(6163..6188)
                     /label="SL011- Sequencing from pRSV rev"
                     /note="sequence: CAGGGTGCACACCAATGTGGTGAATG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             6199..6576
                     /label="H2B"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(6348..6369)
                     /label="MH036"
                     /note="sequence: ggacgaaatgccggtgtcaggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    6577..6594
                     /label="linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             6577..6594
                     /label="Translation 6577-6594"
     primer          complement(6580..6639)
                     /label="SL023- H2B Gibson rev mCherry"
                     /note="sequence: ctccttgatgatggccatgttatcctcctcgcccttgctcaccatggtggcgaccggtgg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          6595..6612
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             6595..7305
                     /label="mCherry"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             6595..7305
                     /label="Translation 6595-7305"
     primer          6633..6657
                     /label="SL041- mcherry fwd"
                     /note="sequence: ggagttcatgcgcttcaaggtgcac"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    6846..6846
                     /label="mutation c->t"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(6918..6937)
                     /label="QB2267"
                     /note="sequence: cctgggtcacggtcaccacg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          7276..7335
                     /label="SL024 - poly Gibson fwd mCherry"
                     /note="sequence: accggcggcatggacgagctgtacaagtcttaagcgactctagatcataatcagccatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(7282..7302)
                     /label="BFP R"
                     /note="sequence: CTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(7284..7305)
                     /label="SL026 - mCherry rev"
                     /note="sequence: agacttgtacagctcgtccatg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7299..7321
                     /label="SL033-seq from end of mcherry dual reporter"
                     /note="sequence: caagtcttaagcgactctagatc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    7318..7543
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7327..7359
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    7455..7505
                     /label="SV40\pA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(7514..7573)
                     /label="SL002 SV40pA Gibson"
                     /note="sequence: gttattgtctcatgagcggatacacatatgagatacattgatgagtttggacaaaccaca"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    7544..7549
                     /label="inserted NdeI (for color switching)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7558..7585)
                     /label="MH008"
                     /note="sequence: ctaaagggaacaaaagctggagctccaccctcgcatttatcagggttattgtctcatgag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    7658..8445
                     /label="Amp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          7853..7873
                     /label="SL037-Amp fwd"
                     /note="sequence: gtattgacgccgggcaagagc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(7853..7876)
                     /label="SL008 Sequencing from Amp rev"
                     /note="sequence: gttgctcttgcccggcgtcaatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
ORIGIN
        1 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
       61 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
      121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
      181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
      241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
      301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
      361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      781 atgttctttc ctgcggtacc gatcataatc agccatacca catttgtaga ggttttactt
      841 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
      901 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      961 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     1021 gtatcttatc atgtctggat cataatcagc cataccacat ttgtagaggt tttacttgct
     1081 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     1141 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     1201 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     1261 tcttatcatg tctgctcgag gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg
     1321 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc
     1381 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag
     1441 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg
     1501 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg
     1561 tcgacggtat cgataagctt acgcgtgcta gcgcatgcag atctactagt cggccgGATA
     1621 AAgatatcaa ctcgagatgg gatcggccat tgaacaagat ggattgcacg caggttctcc
     1681 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     1741 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     1801 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
     1861 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     1921 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     1981 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     2041 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     2101 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     2161 caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg
     2221 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     2281 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     2341 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     2401 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatcaat tctctagagc
     2461 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     2521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     2581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     2641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     2701 ggcttctgag gcggaaagaa ccagctgggg gctagcaaag cctaggaaga cgtacgtata
     2761 cgcgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag
     2821 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
     2881 atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt
     2941 atgtcgaggt aggcgtgtac ggtgggaagg ccgctctagc ccgggcggat cccccgggct
     3001 gcaggaattc gatatcaagc ttgccaccat gccagagcca gcgaagtctg ctcccgcccc
     3061 gaaaaagggc tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa
     3121 gcgcagccgc aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc
     3181 tgacaccggc atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt
     3241 cgagcgcatc gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac
     3301 ctccagggag atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc
     3361 cgtgtccgag ggtactaagg ccatcaccaa gtacaccagc gctaaggatc cccgggtacc
     3421 ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     3481 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     3541 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     3601 ctggcccacc ctcgtgacca ccttcggcta cggcctgatg tgcttcgccc gctaccccga
     3661 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     3721 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     3781 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     3841 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     3901 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     3961 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     4021 cgacaaccac tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga
     4081 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     4141 gtacaagtag gtcgacctcg agggggggcc cggtaccagg taagtgtacc caattcgccc
     4201 tatagtgagt cgtattacaa ttcactcgat cgcccttccc aacagttgcg cagcctgaat
     4261 ggcgaatgga gatccaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     4321 gtgtatttta gattcacagt cccaaggctc atttcaggcc cctcagtcct cacagtctgt
     4381 tcatgatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     4441 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     4501 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     4561 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct taacgcgtaa
     4621 attgtaagcg tctcatgaag acgacgcgaa attcagcatt ttcacaagcg ttatctttta
     4681 caaaaccgat ctcactctcc tttgatgcga atgccagcgt cagacatcat atgcagatac
     4741 tcacctgcat cctgaaccca ttgacctcca accccgtaat agcgatgcgt aatgatgtcg
     4801 atagttacta acgggtcttg ttcgattaac tgccgcagaa actcttccag gtcaccagtg
     4861 cagtgcttga taacaggagt cttcccagga tggcgaacaa caagaaactg gtttccgtct
     4921 tcacggactt cgttgctttc cagtttagca atacgcttac tcccatccga gataacacct
     4981 tcgtaatact cacgctgctc gttgagtttt gattttgctg tttcaagctc aacacgcagt
     5041 ttccctactg ttagcgcaat atcctcgttc tcctggtcgc ggcgtttgat gtattgctgg
     5101 tttctttccc gttcatccag cagttccagc acaatcgatg gtgttaccaa ttcatggaaa
     5161 aggtctgcgt caaatcccca gtcgtcatgc attgcctgct ctgccgcttc acgcagtgcc
     5221 tgagagttaa tttcgctcac ttcgaacctc tctgtttact gataagttcc agatcctcct
     5281 ggcaacttgc acaagtccga caaccctgaa cgaccaggcg tcttcgttca tctatcggat
     5341 cgccacactc acaacaatga gtggcagata tagcctggtg gttcaggcgg cgcattttta
     5401 ttgctgtgtt gcgctgtaat tcttctattt ctgatgctga atcaatgatg tctgccatct
     5461 ttcattaatc cctgaactgt tggttaatac gcttgagggt gaatgcgaat aataaaaaag
     5521 gagcctgtag ctccctgatg attttgcttt tcatgttcat cgttccttaa agacgccgtt
     5581 taacatgccg attgccaggc ttaaatgagt cggtgtgaat cccatcagcg ttaccgtttc
     5641 gcggtgcttc ttcagtacgc tacggcaaat gtcatcgacg tttttatccg gaaactgctg
     5701 tctggctttt tttgatttca gaattagcct gacgggcaat gctgcgaagg gcgttttcct
     5761 gctgaggtgt cattgaacaa gtcccatgtc ggcaagcata agcacacaga atatgaagcc
     5821 cgctgccaga aaaatgcatt ccgtggttgt catacctggt ttctctcatc tgcttctgct
     5881 ttcgccacca tcatttccag ctttcctagg gacgtccata tgAATGTAGT CTTATGCAAT
     5941 ACACTTGTAG TCTTGCAACA TGGTAACGAT GAGTTAGCAA CATGCCTTAC AAGGAGAGAA
     6001 AAAGCACCGT GCATGCCGAT TGGTGGAAGT AAGGTGGTAC GATCGTGCCT TATTAGGAAG
     6061 GCAACAGACA GGTCTGACAT GGATTGGACG AACCACTGAA TTGCGCATTG CAGAGATAAT
     6121 TGTATTTAAG TGCCTAGCTC GATACAATAA ACGCCATTTG ACCATTCACC ACATTGGTGT
     6181 GCACCCTGCA Gatctagaat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
     6241 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
     6301 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
     6361 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
     6421 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
     6481 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
     6541 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
     6601 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     6661 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     6721 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     6781 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     6841 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     6901 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     6961 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     7021 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     7081 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     7141 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     7201 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     7261 gagggccgcc actccaccgg cggcatggac gagctgtaca agtcttaagc gactctagat
     7321 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     7381 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     7441 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7501 actgcattct agttgtggtt tgtccaaact catcaatgta tctcatatgt gtatccgctc
     7561 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     7621 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     7681 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     7741 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     7801 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     7861 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     7921 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     7981 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     8041 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     8101 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     8161 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     8221 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     8281 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     8341 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     8401 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     8461 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagatt
//