LOCUS       pMH004_phiC31_neo-HS4- 11067 bp ds-DNA     circular     18-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    1..273
                     /label="phiC31attB"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    331..336
                     /label="insertion found from sequencing"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    351..1151
                     /label="NeoR"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          426..447
                     /label="MH023"
                     /note="sequence: gactgggcacaacagacaatcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(673..699)
                     /label="SL009- Sequencing from Neo (check for phiC31)"
                     /note="sequence: atactttctcggcaggagcaaggtgag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          1019..1045
                     /label="SL010- Sequencing from Neo fwd (TRE check)"
                     /note="sequence: catagcgttggctacccgtgatattgc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    1168..1444
                     /label="BGHpA"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          1434..1493
                     /label="MH015"
                     /note="sequence: accagctgggggctagcaaagcctaggaagacgtaaattgtaagcgtgagatccgtttcg"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1487..2728
                     /label="HS4"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    1506..1735
                     /label="core Felsenfeld 1997"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(2735..2794)
                     /label="MH016"
                     /note="sequence: ctgatagggagtaaactcgacgcgtatactcaataatcaatgtcATCTCGACtctagagg"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    2743..2750
                     /label="insertion"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2776..2977
                     /label="TRE-tight"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    2785..2803
                     /label="tetO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    2821..2839
                     /label="tetO"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    2857..2875
                     /label="tetO"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    2892..2910
                     /label="tetO"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2928..2946
                     /label="tetO"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     promoter        2984..4138
                     /label="PEF-1alpha"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3030..3051)
                     /label="MH024"
                     /note="sequence: ctctaggcaccggttcaattgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3258..3277)
                     /label="SL029-seq from pEF rev"
                     /note="sequence: ctgcagccaggtggaagtaa"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          3970..3990
                     /label="SL028-seq from pEF fwd"
                     /note="sequence: ttatgcgatggagtttcccca"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer_bind     4162..4181
                     /label="T3\Primer"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    4265..4270
                     /label="Kozak"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    4271..4648
                     /label="H2B"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          4673..4690
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    4673..5389
                     /label="citrine"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          5270..5291
                     /label="MH013"
                     /note="sequence: cactacctgagctaccagtccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          5422..5449
                     /label="SL020-Lambda Gibson seq fwd"
                     /note="sequence: gtaagtgtacccaattcgccctatagtg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          5439..5460
                     /label="T7\Primer"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    5472..5855
                     /label="SV40\pA"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          5636..5668
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          5846..5905
                     /label="MH009"
                     /note="sequence: tgtatcttaacgcgtaaattgtaagcgtctcagtaaattgtaagcgtgagatccgtttcg"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     primer          5878..5905
                     /label="MH009 binding"
                     /note="sequence: gtaaattgtaagcgtgagatccgtttcg"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5899..7140
                     /label="HS4"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    5918..6147
                     /label="core Felsenfeld 1997"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7147..7177)
                     /label="MH010 binding"
                     /note="sequence: tcaataatcaatgtcATCTCGACtctagagg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          complement(7147..7206)
                     /label="MH010"
                     /note="sequence: AAGACTACATTcatatggacgtccctaggtcaataatcaatgtcATCTCGACtctagagg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    7155..7162
                     /label="insertion"
                     /ApEinfo_revcolor=#85dae9
                     /ApEinfo_fwdcolor=#85dae9
     misc_feature    7178..7195
                     /label="inserted MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     misc_feature    7196..7458
                     /label="RSV Promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(7425..7446)
                     /label="MH014"
                     /note="sequence: TGTGGTGAATGGTCAAATGGCG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7436..7461)
                     /label="SL011- Sequencing from pRSV rev"
                     /note="sequence: CAGGGTGCACACCAATGTGGTGAATG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             7472..7849
                     /label="H2B"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     misc_feature    7850..7867
                     /label="linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             7850..7867
                     /label="Translation 7850-7867"
     primer          complement(7853..7912)
                     /label="SL023- H2B Gibson rev mCherry"
                     /note="sequence: ctccttgatgatggccatgttatcctcctcgcccttgctcaccatggtggcgaccggtgg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          7868..7885
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             7868..8578
                     /label="mCherry"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             7868..8578
                     /label="Translation 7868-8578"
     misc_feature    8119..8119
                     /label="mutation c->t"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8191..8210)
                     /label="QB2267"
                     /note="sequence: cctgggtcacggtcaccacg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          8549..8608
                     /label="SL024 - poly Gibson fwd mCherry"
                     /note="sequence: accggcggcatggacgagctgtacaagtcttaagcgactctagatcataatcagccatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(8555..8575)
                     /label="BFP R"
                     /note="sequence: CTTGTACAGCTCGTCCATGCC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(8557..8578)
                     /label="SL026 - mCherry rev"
                     /note="sequence: agacttgtacagctcgtccatg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          8572..8594
                     /label="SL033-seq from end of mcherry dual reporter"
                     /note="sequence: caagtcttaagcgactctagatc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8591..8816
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          8600..8632
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    8728..8778
                     /label="SV40\pA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8787..8846)
                     /label="SL002 SV40pA Gibson"
                     /note="sequence: gttattgtctcatgagcggatacacatatgagatacattgatgagtttggacaaaccaca"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8817..8822
                     /label="inserted NdeI (for color switching)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    8931..9718
                     /label="Amp"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(9126..9149)
                     /label="SL008 Sequencing from Amp rev"
                     /note="sequence: gttgctcttgcccggcgtcaatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     rep_origin      9840..10522
                     /label="Ori"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          10591..10623
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          10828..10860
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
ORIGIN
        1 gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct tgggctcccc
       61 gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga ggtggcggta
      121 gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac cgctgggcgc
      181 ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata cgcggggcag
      241 cgtcagcggg ttctcgacgg tcacggcggg catgtcgacg gtatcgataa gcttacgcgt
      301 gctagcgcat gcagatctac tagtcggccg GATAAAgata tcaactcgag atgggatcgg
      361 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      421 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
      481 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
      541 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
      601 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
      661 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
      721 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
      781 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
      841 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
      901 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
      961 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1021 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1081 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1141 acgagttctt ctgaggggat caattctcta gagctcgctg atcagcctcg actgtgcctt
     1201 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
     1261 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
     1321 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
     1381 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
     1441 gggggctagc aaagcctagg aagacgtaaa ttgtaagcgt gagatccgtt tcgataagct
     1501 ctagagggac agcccccccc caaagccccc agggatgtaa ttacgtccct cccccgctag
     1561 ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc gcatccccga
     1621 gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc gctttcctct
     1681 gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg gaaaaagctt
     1741 taggctgaaa gagagattta gaatgacaga atcatagaac ggcctgggtt gcaaaggagc
     1801 acagtgctca tccagatcca accccctgct atgtgcaggg tcatcaacca gcagcccagg
     1861 ctgcccagag ccacatccag cctggccttg aatgcctgca gggatggggc atccacagcc
     1921 tccttgggca acctgttcag tgcgtcacca ccctctgggg gaaaaactgc ctcctcatat
     1981 ccaacccaaa cctcccctgt ctcagtgtaa agccattccc ccttgtccta tcaaggggga
     2041 gtttgctgtg acattgttgg tctggggtga cacatgtttg ccaattcagt gcatcacgga
     2101 gaggcagatc ttggggataa ggaagtgcag gacagcatgg acgtgggaca tgcaggtgtt
     2161 gagggctctg ggacactctc caagtcacag cgttcagaac agccttaagg ataagaagat
     2221 aggatagaag gacaaagagc aagttaaaac ccagcatgga gaggagcaca aaaaggccac
     2281 agacactgct ggtccctgtg tctgagcctg catgtttgat ggtgtctgga tgcaagcaga
     2341 aggggtggaa gagcttgcct ggagagatac agctgggtca gtaggactgg gacaggcagc
     2401 tggagaattg ccatgtagat gttcatacaa tcgtcaaatc atgaaggctg gaaaagccct
     2461 ccaagatccc caagaccaac cccaacccac ccaccgtgcc cactggccat gtccctcagt
     2521 gccacatccc cacagttctt catcacctcc agggacggtg acccccccac ctccgtgggc
     2581 agctgtgcca ctgcagcacc gctctttgga gaaggtaaat cttgctaaat ccagcccgac
     2641 cctcccctgg cacaacgtaa ggccattatc tctcatccaa ctccaggacg gagtcagtga
     2701 ggatggggct ctagcggggg gatcaaacgg ccggcctcta gaGTCGAGAT gacattgatt
     2761 attgagtata cgcgtcgagt ttactcccta tcagtgatag agaacgtatg tcgagtttac
     2821 tccctatcag tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta
     2881 tgtcgagttt atccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga
     2941 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaatg catgagcgca catcgcccac
     3001 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     3061 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     3121 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     3181 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     3241 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     3301 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     3361 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     3421 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     3481 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     3541 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     3601 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     3661 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     3721 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     3781 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     3841 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     3901 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     3961 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     4021 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     4081 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagc
     4141 tagcgattac gccaagctcg aaattaaccc tcactaaagg gaacaaaagc tggagctcca
     4201 ccgcggtggc ggccgctcta gcccgggcgg atcccccggg ctgcaggaat tcgatatcaa
     4261 gcttgccacc atgccagagc cagcgaagtc tgctcccgcc ccgaaaaagg gctccaagaa
     4321 ggcggtgact aaggcgcaga agaaaggcgg caagaagcgc aagcgcagcc gcaaggagag
     4381 ctattccatc tatgtgtaca aggttctgaa gcaggtccac cctgacaccg gcatttcgtc
     4441 caaggccatg ggcatcatga attcgtttgt gaacgacatt ttcgagcgca tcgcaggtga
     4501 ggcttcccgc ctggcgcatt acaacaagcg ctcgaccatc acctccaggg agatccagac
     4561 ggccgtgcgc ctgctgctgc ctggggagtt ggccaagcac gccgtgtccg agggtactaa
     4621 ggccatcacc aagtacacca gcgctaagga tccccgggta ccggtcgcca ccatggtgag
     4681 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     4741 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     4801 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     4861 caccttcggc tacggcctga tgtgcttcgc ccgctacccc gaccacatga agcagcacga
     4921 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     4981 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     5041 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     5101 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     5161 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     5221 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     5281 ctaccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     5341 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt aggtcgacct
     5401 cgaggggggg cccggtacca ggtaagtgta cccaattcgc cctatagtga gtcgtattac
     5461 aattcactcg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gagatccaat
     5521 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattcaca
     5581 gtcccaaggc tcatttcagg cccctcagtc ctcacagtct gttcatgatc ataatcagcc
     5641 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     5701 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     5761 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     5821 gttgtggttt gtccaaactc atcaatgtat cttaacgcgt aaattgtaag cgtctcagta
     5881 aattgtaagc gtgagatccg tttcgataag ctctagaggg acagcccccc cccaaagccc
     5941 ccagggatgt aattacgtcc ctcccccgct agggggcagc agcgagccgc ccggggctcc
     6001 gctccggtcc ggcgctcccc ccgcatcccc gagccggcag cgtgcgggga cagcccgggc
     6061 acggggaagg tggcacggga tcgctttcct ctgaacgctt ctcgctgctc tttgagcctg
     6121 cagacacctg gggggatacg gggaaaaagc tttaggctga aagagagatt tagaatgaca
     6181 gaatcataga acggcctggg ttgcaaagga gcacagtgct catccagatc caaccccctg
     6241 ctatgtgcag ggtcatcaac cagcagccca ggctgcccag agccacatcc agcctggcct
     6301 tgaatgcctg cagggatggg gcatccacag cctccttggg caacctgttc agtgcgtcac
     6361 caccctctgg gggaaaaact gcctcctcat atccaaccca aacctcccct gtctcagtgt
     6421 aaagccattc ccccttgtcc tatcaagggg gagtttgctg tgacattgtt ggtctggggt
     6481 gacacatgtt tgccaattca gtgcatcacg gagaggcaga tcttggggat aaggaagtgc
     6541 aggacagcat ggacgtggga catgcaggtg ttgagggctc tgggacactc tccaagtcac
     6601 agcgttcaga acagccttaa ggataagaag ataggataga aggacaaaga gcaagttaaa
     6661 acccagcatg gagaggagca caaaaaggcc acagacactg ctggtccctg tgtctgagcc
     6721 tgcatgtttg atggtgtctg gatgcaagca gaaggggtgg aagagcttgc ctggagagat
     6781 acagctgggt cagtaggact gggacaggca gctggagaat tgccatgtag atgttcatac
     6841 aatcgtcaaa tcatgaaggc tggaaaagcc ctccaagatc cccaagacca accccaaccc
     6901 acccaccgtg cccactggcc atgtccctca gtgccacatc cccacagttc ttcatcacct
     6961 ccagggacgg tgaccccccc acctccgtgg gcagctgtgc cactgcagca ccgctctttg
     7021 gagaaggtaa atcttgctaa atccagcccg accctcccct ggcacaacgt aaggccatta
     7081 tctctcatcc aactccagga cggagtcagt gaggatgggg ctctagcggg gggatcaaac
     7141 ggccggcctc tagaGTCGAG ATgacattga ttattgacct agggacgtcc atatgAATGT
     7201 AGTCTTATGC AATACACTTG TAGTCTTGCA ACATGGTAAC GATGAGTTAG CAACATGCCT
     7261 TACAAGGAGA GAAAAAGCAC CGTGCATGCC GATTGGTGGA AGTAAGGTGG TACGATCGTG
     7321 CCTTATTAGG AAGGCAACAG ACAGGTCTGA CATGGATTGG ACGAACCACT GAATTGCGCA
     7381 TTGCAGAGAT AATTGTATTT AAGTGCCTAG CTCGATACAA TAAACGCCAT TTGACCATTC
     7441 ACCACATTGG TGTGCACCCT GCAGatctag aatgccagag ccagcgaagt ctgctcccgc
     7501 cccgaaaaag ggctccaaga aggcggtgac taaggcgcag aagaaaggcg gcaagaagcg
     7561 caagcgcagc cgcaaggaga gctattccat ctatgtgtac aaggttctga agcaggtcca
     7621 ccctgacacc ggcatttcgt ccaaggccat gggcatcatg aattcgtttg tgaacgacat
     7681 tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat tacaacaagc gctcgaccat
     7741 cacctccagg gagatccaga cggccgtgcg cctgctgctg cctggggagt tggccaagca
     7801 cgccgtgtcc gagggtacta aggccatcac caagtacacc agcgctaagg atccaccggt
     7861 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
     7921 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
     7981 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
     8041 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
     8101 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
     8161 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
     8221 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
     8281 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
     8341 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
     8401 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
     8461 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     8521 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtctta
     8581 agcgactcta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     8641 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     8701 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     8761 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatctcata
     8821 tgtgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8881 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8941 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9001 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9061 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9121 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9181 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9241 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9301 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9361 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9421 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9481 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9541 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9601 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9661 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9721 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9781 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9841 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9901 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9961 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    10021 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    10081 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    10141 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    10201 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    10261 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    10321 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    10381 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    10441 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    10501 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    10561 catgttcttt cctgcggtac cgatcataat cagccatacc acatttgtag aggttttact
    10621 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10681 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10741 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    10801 tgtatcttat catgtctgga tcataatcag ccataccaca tttgtagagg ttttacttgc
    10861 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
    10921 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    10981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    11041 atcttatcat gtctgctcga ggtcgac
//