LOCUS       pMH012_AAVS1_puro-pA-9 10297 bp ds-DNA     circular     18-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          488..509
                     /label="SL047 - Seq from pOri FWD"
                     /note="sequence: gggggaaacgcctggtatcttt"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    877..898
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        913..918
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        919..936
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        937..943
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          942..961
                     /label="p82 -PB rev"
                     /note="sequence: tgtgtggaattgtgagcgga"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(942..961)
                     /label="p81-PB fwd"
                     /note="sequence: tccgctcacaattccacaca"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    951..967
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer_bind     975..991
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     promoter        1012..1030
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1065..1868
                     /label="left_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1875..1900
                     /label="SA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1924..1977
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1987..2586
                     /label="PuroR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    2624..2848
                     /label="polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2836..2857
                     /label="MH025 binding"
                     /note="sequence: gtgggctctatggcttctgagg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2876..2913
                     /label="MH037"
                     /note="sequence: ggctagcaaagccttcgaacagctgcatatagcactgc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(2876..2913)
                     /label="MH038"
                     /note="sequence: gcagtgctatatgcagctgttcgaaggctttgctagcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2889..2894
                     /label="Inserted Cutsite"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2904..3227
                     /label="9xTRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    2921..2939
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    2957..2975
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    2993..3011
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3029..3047
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3065..3083
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3101..3119
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3137..3155
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3173..3191
                     /label="TRE"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3209..3227
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        3265..4419
                     /label="pEF"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3311..3332)
                     /label="MH024"
                     /note="sequence: ctctaggcaccggttcaattgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3539..3558)
                     /label="SL030-seq from pEF rev"
                     /note="sequence: ctgcagccaggtggaagtaa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3539..3558)
                     /label="SL029-seq from pEF rev"
                     /note="sequence: ctgcagccaggtggaagtaa"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          4251..4271
                     /label="SL028-seq from pEF fwd"
                     /note="sequence: ttatgcgatggagtttcccca"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4465..4483)
                     /label="MH001"
                     /note="sequence: GACATTGATTATTGACTcGTaCgAGGGTGGAGCTCCAGCTTTTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4546..4551
                     /label="Kozak"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4552..4929
                     /label="H2B"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(4701..4722)
                     /label="MH036"
                     /note="sequence: ggacgaaatgccggtgtcaggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             4930..4953
                     /label="Translation 4930-4953"
     primer          4954..4971
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          4954..4973
                     /label="Citrine F1"
                     /note="sequence: gatccaccggtcgccaccatggtgagcaagggcgagga"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    4954..5670
                     /label="Citrine"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             4954..5670
                     /label="Translation 4954-5670"
     primer          5551..5572
                     /label="MH013"
                     /note="sequence: cactacctgagctaccagtccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(5649..5670)
                     /label="Citrine R1"
                     /note="sequence: tgactttgctcttgtccagtctagatgggccaggattctcctcgacgtctccgcaagttaggaggctccccctgccctccttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(5652..5671)
                     /label="SL026 - mCherry rev"
                     /note="sequence: agacttgtacagctcgtccatg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             5671..5673
                     /label="Translation 5671-5673"
     primer          5703..5730
                     /label="SL020-Lambda Gibson seq fwd"
                     /note="sequence: gtaagtgtacccaattcgccctatagtg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    5753..6136
                     /label="SV40/pA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          5917..5949
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          6111..6170
                     /label="MH041"
                     /note="sequence: tgtccaaactcatcaatgtatcttaatctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6137..6170
                     /label="MH011"
                     /note="sequence: gtatcttaacgcgtaaattgtaagcgtctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          6137..6170
                     /label="MH041 binding"
                     /note="sequence: tctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6142..6170
                     /label="MH034"
                     /note="sequence: GTATTGCATAAGACTACATTcatatggacgtaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6142..6170
                     /label="MH017"
                     /note="sequence: ccagctgggggctagcaaagcctaggaagacaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          6142..6170
                     /label="MH011 binding"
                     /note="sequence: aagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          6142..6170
                     /label="MH021"
                     /note="sequence: attgtaagcgtctcaaatacaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          6142..6170
                     /label="MH045"
                     /note="sequence: gaaagaaccagctgggggctagcaaagccttaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     enhancer        6160..6391
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(6395..6417)
                     /label="MH012 binding"
                     /note="sequence: gcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6395..6417)
                     /label="MH033"
                     /note="sequence: atcaatgtatcttaacgcgtaaattgtaagcgtctcagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(6395..6417)
                     /label="MH042"
                     /note="sequence: gttgtggtttgtccaaactcatcaatgtatcttaacagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6395..6417)
                     /label="MH018"
                     /note="sequence: ctctatcactgatagggagtaaactcgacgcgtatacgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(6395..6417)
                     /label="MH046"
                     /note="sequence: tgatagggagtaaactgcagtgctatatgcagctgttgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(6395..6417)
                     /label="MH022"
                     /note="sequence: TTcatatggacgtcgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6395..6417)
                     /label="MH042 binding"
                     /note="sequence: cagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(6395..6454)
                     /label="MH012"
                     /note="sequence: TATTGCATAAGACTACATTcatatggacgtccctagggcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6418..6435
                     /label="inserted MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          6418..6444
                     /label="SL018-Lambda DNA fwd (250bp) Gibson"
                     /note="sequence: cgatcccggtacgctgcaggataatgtcaatacctagggacgtccatatgAATGTAGTC"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6418..6468
                     /label="SL001 MCS pRSV Gibson"
                     /note="sequence: gtaagcgtctcaaatacctagggacgtccatatgAATGTAGTCTTATGCAATACACTTGTAGTCTTG"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          6424..6465
                     /label="SL001 MCS pRSV Gibson"
                     /note="sequence: caaatacctaggttcgaagacgtccatatgAATGTAGTCTTATGCAATACACTTGTAGTC"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          6434..6468
                     /label="MH007"
                     /note="sequence: aGTCGAGATgacattgattattgactcAATGTAGTCTTATGCAATACACTTGTAGTCTTG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6436..6698
                     /label="RSV Promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6665..6686)
                     /label="MH014"
                     /note="sequence: TGTGGTGAATGGTCAAATGGCG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(6676..6701)
                     /label="SL011- Sequencing from pRSV rev"
                     /note="sequence: CAGGGTGCACACCAATGTGGTGAATG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             6712..7089
                     /label="H2B"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(6861..6882)
                     /label="MH036"
                     /note="sequence: ggacgaaatgccggtgtcaggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7082..7110)
                     /label="SL021 - H2B Gibson rev"
                     /note="sequence: GGCCGAATGCTGAGGTAGTCAGCGGTACCGACATggtggcgaccggtggatccttagcgc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     misc_feature    7090..7107
                     /label="linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             7090..7107
                     /label="Translation 7090-7107"
     primer          complement(7093..7152)
                     /label="SL023- H2B Gibson rev mCherry"
                     /note="sequence: ctccttgatgatggccatgttatcctcctcgcccttgctcaccatggtggcgaccggtgg"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          7108..7125
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             7108..7818
                     /label="mCherry"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             7108..7818
                     /label="Translation 7108-7818"
     primer          complement(7149..7170)
                     /label="SL040- mcherry Gibson rev"
                     /note="sequence: caccttgaagcgcatgaactcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7149..7173
                     /label="SL041- mcherry fwd"
                     /note="sequence: ggagttcatgcgcttcaaggtgcac"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    7359..7359
                     /label="mutation c->t"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(7431..7450)
                     /label="QB2267"
                     /note="sequence: cctgggtcacggtcaccacg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          7789..7848
                     /label="SL024 - poly Gibson fwd mCherry"
                     /note="sequence: accggcggcatggacgagctgtacaagtcttaagcgactctagatcataatcagccatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(7797..7818)
                     /label="SL026 - mCherry rev"
                     /note="sequence: agacttgtacagctcgtccatg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7812..7834
                     /label="SL033-seq from end of mcherry dual reporter"
                     /note="sequence: caagtcttaagcgactctagatc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          7819..7848
                     /label="SL022 - polyA Gibson fwd"
                     /note="sequence: CTGGAGAGCTTTGCACAGTCTCAGTCCAAAtaagcgactctagatcataatcagccatac"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    7831..8056
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7840..7872
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     polyA_signal    7968..8018
                     /label="SV40\pA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8027..8086)
                     /label="SL002 SV40pA Gibson"
                     /note="sequence: gttattgtctcatgagcggatacacatatgagatacattgatgagtttggacaaaccaca"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(8038..8063)
                     /label="SL032-H2B mCherry Gibson rev"
                     /note="sequence: gtctctagaggccgagacgcttacaatttacatatgagatacattgatgagtttg"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     misc_feature    8057..8062
                     /label="inserted NdeI (for color switching)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(8071..8098)
                     /label="MH008"
                     /note="sequence: ctaaagggaacaaaagctggagctccaccctcgcatttatcagggttattgtctcatgag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(8142..8160)
                     /label="MH040 binding"
                     /note="sequence: gggaataagggcgacacgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(8142..8201)
                     /label="MH040"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(8142..8201)
                     /label="MH043"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    8203..9039
                     /label="right_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(9314..10105)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          9910..9933
                     /label="SL008 Sequencing from Amp rev"
                     /note="sequence: gttgctcttgcccggcgtcaatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(9913..9933)
                     /label="SL037-Amp fwd"
                     /note="sequence: gtattgacgccgggcaagagc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(10106..10174)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          10138..10157
                     /label="MH040"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          10138..10157
                     /label="MH043"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          10139..10157
                     /label="MH040 binding"
                     /note="sequence: gggaataagggcgacacgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt ctctgaccag
     1081 cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct
     1141 acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct ccactccctc
     1201 ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc acttccttct
     1261 cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg tcctctccgg
     1321 gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc ccttttcctt
     1381 ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg acggatgtct
     1441 cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt ctggacaacc
     1501 ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt tctttgcctg
     1561 gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg ggcagctccc
     1621 ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca tggcatcttc
     1681 caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat gtccacttca
     1741 ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc accttatatt
     1801 cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc tccaccccac
     1861 agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag atctggcagc
     1921 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     1981 ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2581 gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcta
     2881 gcaaagcctt cgaacagctg catatagcac tgcagtttac tccctatcag tgatagagag
     2941 tcaaaaggcc atgccctccc tatcagtgat agagagagca gactcaaggt ggtccctatc
     3001 agtgatagag agattgagca agagggcctc cctatcagtg atagagaata gcactgcagt
     3061 ttactcccta tcagtgatag agagtcaaaa ggccatgccc tccctatcag tgatagagag
     3121 agcagactca aggtggtccc tatcagtgat agagagattg agcaagaggg cctccctatc
     3181 agtgatagag attgatccag tcagggcctc cctatcagtg atagagaagc tatgtgcagg
     3241 tagggctgta cagtgggaat gcatgagcgc acatcgccca cagtccccga gaagttgggg
     3301 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3361 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3421 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     3481 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3541 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3781 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     3841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     4021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     4081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     4141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     4201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     4261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     4381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagcgatta cgccaagctc
     4441 gaaattaacc ctcactaaag ggaacaaaag ctggagctcc accgcggtgg cggccgctct
     4501 agcccgggcg gatcccccgg gctgcaggaa ttcgatatca agcttgccac catgccagag
     4561 ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag
     4621 aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac
     4681 aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg
     4741 aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat
     4801 tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg
     4861 cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc
     4921 agcgctaagg atccccgggt accggtcgcc accatggtga gcaagggcga ggagctgttc
     4981 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
     5041 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     5101 accaccggca agctgcccgt gccctggccc accctcgtga ccaccttcgg ctacggcctg
     5161 atgtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
     5221 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
     5281 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
     5341 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
     5401 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
     5461 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
     5521 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
     5581 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
     5641 atcactctcg gcatggacga gctgtacaag taggtcgacc tcgagggggg gcccggtacc
     5701 aggtaagtgt acccaattcg ccctatagtg agtcgtatta caattcactc gatcgccctt
     5761 cccaacagtt gcgcagcctg aatggcgaat ggagatccaa tttttaagtg tataatgtgt
     5821 taaactactg attctaattg tttgtgtatt ttagattcac agtcccaagg ctcatttcag
     5881 gcccctcagt cctcacagtc tgttcatgat cataatcagc cataccacat ttgtagaggt
     5941 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     6001 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     6061 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     6121 catcaatgta tcttaatctc aaagtaacaa aacttttatg agggacagcc cccccccaaa
     6181 gcccccaggg atgtaattac gtccctcccc cgctaggggg cagcagcgag ccgcccgggg
     6241 ctccgctccg gtccggcgct ccccccgcat ccccgagccg gcagcgtgcg gggacagccc
     6301 gggcacgggg aaggtggcac gggatcgctt tcctctgaac gcttctcgct gctctttgag
     6361 cctgcagaca cctgggggga tacggggaaa aggcctccac ggccactagt attatgccct
     6421 agggacgtcc atatgAATGT AGTCTTATGC AATACACTTG TAGTCTTGCA ACATGGTAAC
     6481 GATGAGTTAG CAACATGCCT TACAAGGAGA GAAAAAGCAC CGTGCATGCC GATTGGTGGA
     6541 AGTAAGGTGG TACGATCGTG CCTTATTAGG AAGGCAACAG ACAGGTCTGA CATGGATTGG
     6601 ACGAACCACT GAATTGCGCA TTGCAGAGAT AATTGTATTT AAGTGCCTAG CTCGATACAA
     6661 TAAACGCCAT TTGACCATTC ACCACATTGG TGTGCACCCT GCAGatctag aatgccagag
     6721 ccagcgaagt ctgctcccgc cccgaaaaag ggctccaaga aggcggtgac taaggcgcag
     6781 aagaaaggcg gcaagaagcg caagcgcagc cgcaaggaga gctattccat ctatgtgtac
     6841 aaggttctga agcaggtcca ccctgacacc ggcatttcgt ccaaggccat gggcatcatg
     6901 aattcgtttg tgaacgacat tttcgagcgc atcgcaggtg aggcttcccg cctggcgcat
     6961 tacaacaagc gctcgaccat cacctccagg gagatccaga cggccgtgcg cctgctgctg
     7021 cctggggagt tggccaagca cgccgtgtcc gagggtacta aggccatcac caagtacacc
     7081 agcgctaagg atccaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
     7141 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7201 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7261 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7321 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     7381 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7441 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7501 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     7561 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     7621 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     7681 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     7741 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     7801 gacgagctgt acaagtctta agcgactcta gatcataatc agccatacca catttgtaga
     7861 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     7921 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     7981 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8041 actcatcaat gtatctcata tgtgtatccg ctcatgagac aataaccctg ataaatgctt
     8101 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     8161 taaattgtaa gcgtctcggc ctctagagac attgattatt gaactaggga caggattggt
     8221 gacagaaaag ccccatcctt aggcctcctc cttcctagtc tcctgatatt gggtctaacc
     8281 cccacctcct gttaggcaga ttccttatct ggtgacacac ccccatttcc tggagccatc
     8341 tctctccttg ccagaacctc taaggtttgc ttacgatgga gccagagagg atcctgggag
     8401 ggagagcttg gcagggggtg ggagggaagg gggggatgcg tgacctgccc ggttctcagt
     8461 ggccaccctg cgctaccctc tcccagaacc tgagctgctc tgacgcggct gtctggtgcg
     8521 tttcactgat cctggtgctg cagcttcctt acacttccca agaggagaag cagtttggaa
     8581 aaacaaaatc agaataagtt ggtcctgagt tctaactttg gctcttcacc tttctagtcc
     8641 ccaatttata ttgttcctcc gtgcgtcagt tttacctgtg agataaggcc agtagccagc
     8701 cccgtcctgg cagggctgtg gtgaggaggg gggtgtccgt gtggaaaact ccctttgtga
     8761 gaatggtgcg tcctaggtgt tcaccaggtc gtggccgcct ctactccctt tctctttctc
     8821 catccttctt tccttaaaga gtccccagtg ctatctggga catattcctc cgcccagagc
     8881 agggtcccgc ttccctaagg ccctgctctg ggcttctggg tttgagtcct tggcaagccc
     8941 aggagaggcg ctcaggcttc cctgtccccc ttcctcgtcc accatctcat gcccctggct
     9001 ctcctgcccc ttccctacag gggttcctgg ctctgctctt cgccttcttg acgagttctt
     9061 ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
     9121 tatttcacac cgcatcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     9181 tatttttcta aatacattca aatatgtatc cgctcatgag attatcaaaa aggatcttca
     9241 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     9301 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     9361 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     9421 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     9481 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     9541 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     9601 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     9661 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     9721 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     9781 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     9841 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     9901 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     9961 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
    10021 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
    10081 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
    10141 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
    10201 gcatttatca gggttattgt ctcatgacca aaatccctta acgtgagttt tcgttccact
    10261 gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//