LOCUS       pMH015_AAVS1_puro-pA-c 10571 bp ds-DNA     circular     18-NOV-2021
DEFINITION  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /label="ori"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          488..509
                     /label="SL047 - Seq from pOri FWD"
                     /note="sequence: gggggaaacgcctggtatcttt"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     protein_bind    877..898
                     /label="CAP binding site"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     promoter        913..918
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        919..936
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     promoter        937..943
                     /label="lac promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          942..961
                     /label="p82 -PB rev"
                     /note="sequence: tgtgtggaattgtgagcgga"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(942..961)
                     /label="p81-PB fwd"
                     /note="sequence: tccgctcacaattccacaca"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     protein_bind    951..967
                     /label="lac operator"
                     /ApEinfo_revcolor=#31849b
                     /ApEinfo_fwdcolor=#31849b
     primer_bind     975..991
                     /label="M13 rev"
                     /ApEinfo_revcolor=#a020f0
                     /ApEinfo_fwdcolor=#a020f0
     promoter        1012..1030
                     /label="T3 promoter"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    1065..1868
                     /label="left_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    1875..1900
                     /label="SA"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             1924..1977
                     /label="T2A"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     CDS             1987..2586
                     /label="PuroR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          2609..2630
                     /label="MH049"
                     /note="sequence: gctgatcagcctcgactgtgcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    2624..2848
                     /label="polyA"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          2834..2857
                     /label="MH025"
                     /note="sequence: aaccagctgggggctagcaaagcctaggaagacgtacggtgggctctatggcttctgagg"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2836..2857
                     /label="MH025 binding"
                     /note="sequence: gtgggctctatggcttctgagg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          2860..2919
                     /label="MH045"
                     /note="sequence: gaaagaaccagctgggggctagcaaagccttaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          2890..2919
                     /label="MH034"
                     /note="sequence: GTATTGCATAAGACTACATTcatatggacgtaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          2891..2919
                     /label="MH041"
                     /note="sequence: tgtccaaactcatcaatgtatcttaatctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          2891..2919
                     /label="MH021"
                     /note="sequence: attgtaagcgtctcaaatacaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          2891..2919
                     /label="MH041 binding"
                     /note="sequence: tctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          2891..2919
                     /label="MH017"
                     /note="sequence: ccagctgggggctagcaaagcctaggaagacaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          2891..2919
                     /label="MH011 binding"
                     /note="sequence: aagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          2891..2919
                     /label="MH011"
                     /note="sequence: gtatcttaacgcgtaaattgtaagcgtctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          2891..2919
                     /label="MH034"
                     /note="sequence: GTATTGCATAAGACTACATTcatatggacgtaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     enhancer        2909..3140
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(3144..3166)
                     /label="MH042 binding"
                     /note="sequence: cagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3144..3166)
                     /label="MH018"
                     /note="sequence: ctctatcactgatagggagtaaactcgacgcgtatacgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3144..3166)
                     /label="MH012 binding"
                     /note="sequence: gcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3144..3166)
                     /label="MH033"
                     /note="sequence: atcaatgtatcttaacgcgtaaattgtaagcgtctcagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(3144..3166)
                     /label="MH022"
                     /note="sequence: TTcatatggacgtcgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3144..3166)
                     /label="MH012"
                     /note="sequence: TATTGCATAAGACTACATTcatatggacgtccctagggcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(3144..3166)
                     /label="MH042"
                     /note="sequence: gttgtggtttgtccaaactcatcaatgtatcttaacagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(3144..3203)
                     /label="MH046"
                     /note="sequence: tgatagggagtaaactgcagtgctatatgcagctgttgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          3167..3187
                     /label="MH037"
                     /note="sequence: ggctagcaaagccttcgaacagctgcatatagcactgc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3178..3501
                     /label="9xTRE"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    3195..3213
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3231..3249
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3267..3285
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3303..3321
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     misc_feature    3339..3357
                     /label="TRE"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    3375..3393
                     /label="TRE"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     misc_feature    3411..3429
                     /label="TRE"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    3447..3465
                     /label="TRE"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     misc_feature    3483..3501
                     /label="TRE"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     promoter        3539..4693
                     /label="pEF"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(3575..3596)
                     /label="MH050"
                     /note="sequence: cggttcaattgccgacccctcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3585..3606)
                     /label="MH024"
                     /note="sequence: ctctaggcaccggttcaattgc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(3813..3832)
                     /label="SL029-seq from pEF rev"
                     /note="sequence: ctgcagccaggtggaagtaa"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(3813..3832)
                     /label="SL030-seq from pEF rev"
                     /note="sequence: ctgcagccaggtggaagtaa"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          4525..4545
                     /label="SL028-seq from pEF fwd"
                     /note="sequence: ttatgcgatggagtttcccca"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(4739..4757)
                     /label="MH001"
                     /note="sequence: GACATTGATTATTGACTcGTaCgAGGGTGGAGCTCCAGCTTTTG"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    4820..4825
                     /label="Kozak"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     misc_feature    4826..5203
                     /label="H2B"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(4975..4996)
                     /label="MH036"
                     /note="sequence: ggacgaaatgccggtgtcaggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          5080..5101
                     /label="MH053"
                     /note="sequence: caagcgctcgaccatcacctcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             5204..5227
                     /label="Translation 5204-5227"
     primer          5228..5245
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     primer          5228..5247
                     /label="Citrine F1"
                     /note="sequence: gatccaccggtcgccaccatggtgagcaagggcgagga"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    5228..5944
                     /label="Citrine"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     CDS             5228..5944
                     /label="Translation 5228-5944"
     primer          5825..5846
                     /label="MH013"
                     /note="sequence: cactacctgagctaccagtccg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(5923..5944)
                     /label="Citrine R1"
                     /note="sequence: tgactttgctcttgtccagtctagatgggccaggattctcctcgacgtctccgcaagttaggaggctccccctgccctccttgtacagctcgtccatgccg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     CDS             5945..5947
                     /label="Translation 5945-5947"
     primer          5977..6004
                     /label="SL020-Lambda Gibson seq fwd"
                     /note="sequence: gtaagtgtacccaattcgccctatagtg"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    6027..6410
                     /label="SV40/pA"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6191..6223
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          6228..6249
                     /label="MH051"
                     /note="sequence: aaaaacctcccacacctccccc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          6385..6444
                     /label="MH041"
                     /note="sequence: tgtccaaactcatcaatgtatcttaatctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          6411..6444
                     /label="MH041 binding"
                     /note="sequence: tctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#9eafd2
                     /ApEinfo_fwdcolor=#9eafd2
     primer          6411..6444
                     /label="MH011"
                     /note="sequence: gtatcttaacgcgtaaattgtaagcgtctcaaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          6416..6444
                     /label="MH017"
                     /note="sequence: ccagctgggggctagcaaagcctaggaagacaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          6416..6444
                     /label="MH034"
                     /note="sequence: GTATTGCATAAGACTACATTcatatggacgtaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          6416..6444
                     /label="MH021"
                     /note="sequence: attgtaagcgtctcaaatacaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          6416..6444
                     /label="MH045"
                     /note="sequence: gaaagaaccagctgggggctagcaaagccttaagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          6416..6444
                     /label="MH011 binding"
                     /note="sequence: aagtaacaaaacttttatgagggacagcc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     enhancer        6434..6665
                     /label="core insul"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(6669..6691)
                     /label="MH012 binding"
                     /note="sequence: gcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6669..6691)
                     /label="MH042 binding"
                     /note="sequence: cagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#b1ff67
                     /ApEinfo_fwdcolor=#b1ff67
     primer          complement(6669..6691)
                     /label="MH046"
                     /note="sequence: tgatagggagtaaactgcagtgctatatgcagctgttgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     primer          complement(6669..6691)
                     /label="MH042"
                     /note="sequence: gttgtggtttgtccaaactcatcaatgtatcttaacagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     primer          complement(6669..6691)
                     /label="MH033"
                     /note="sequence: atcaatgtatcttaacgcgtaaattgtaagcgtctcagcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(6669..6691)
                     /label="MH018"
                     /note="sequence: ctctatcactgatagggagtaaactcgacgcgtatacgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(6669..6691)
                     /label="MH022"
                     /note="sequence: TTcatatggacgtcgcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(6669..6728)
                     /label="MH012"
                     /note="sequence: TATTGCATAAGACTACATTcatatggacgtccctagggcataatactagtggccgtggag"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     misc_feature    6692..6709
                     /label="inserted MCS"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          6710..6742
                     /label="MH007"
                     /note="sequence: aGTCGAGATgacattgattattgactcAATGTAGTCTTATGCAATACACTTGTAGTCTTG"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     misc_feature    6710..6972
                     /label="RSV Promoter"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(6939..6960)
                     /label="MH014"
                     /note="sequence: TGTGGTGAATGGTCAAATGGCG"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(6950..6975)
                     /label="SL011- Sequencing from pRSV rev"
                     /note="sequence: CAGGGTGCACACCAATGTGGTGAATG"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     CDS             6986..7363
                     /label="H2B"
                     /ApEinfo_revcolor=#ffef86
                     /ApEinfo_fwdcolor=#ffef86
     primer          complement(7135..7156)
                     /label="MH036"
                     /note="sequence: ggacgaaatgccggtgtcaggg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          7240..7261
                     /label="MH053"
                     /note="sequence: caagcgctcgaccatcacctcc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     misc_feature    7364..7381
                     /label="linker"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     CDS             7364..7381
                     /label="Translation 7364-7381"
     primer          7382..7399
                     /label="SL025 - mCherry fwd"
                     /note="sequence: atggtgagcaagggcgag"
                     /ApEinfo_revcolor=#d59687
                     /ApEinfo_fwdcolor=#d59687
     CDS             7382..8092
                     /label="mCherry"
                     /ApEinfo_revcolor=#f7977a
                     /ApEinfo_fwdcolor=#f7977a
     CDS             7382..8092
                     /label="Translation 7382-8092"
     primer          complement(7393..7414)
                     /label="MH052"
                     /note="sequence: ggccatgttatcctcctcgccc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(7423..7444)
                     /label="SL040- mcherry Gibson rev"
                     /note="sequence: caccttgaagcgcatgaactcc"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          7423..7447
                     /label="SL041- mcherry fwd"
                     /note="sequence: ggagttcatgcgcttcaaggtgcac"
                     /ApEinfo_revcolor=#faac61
                     /ApEinfo_fwdcolor=#faac61
     misc_feature    7633..7633
                     /label="mutation c->t"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     primer          complement(7705..7724)
                     /label="QB2267"
                     /note="sequence: cctgggtcacggtcaccacg"
                     /ApEinfo_revcolor=#f58a5e
                     /ApEinfo_fwdcolor=#f58a5e
     primer          complement(8071..8092)
                     /label="SL026 - mCherry rev"
                     /note="sequence: agacttgtacagctcgtccatg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          8086..8108
                     /label="SL033-seq from end of mcherry dual reporter"
                     /note="sequence: caagtcttaagcgactctagatc"
                     /ApEinfo_revcolor=#75c6a9
                     /ApEinfo_fwdcolor=#75c6a9
     misc_feature    8105..8330
                     /label="SV40 polyA"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          8114..8146
                     /label="SL007 Sequencing from SV40 fwd"
                     /note="sequence: cagccataccacatttgtagaggttttacttgc"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          8151..8172
                     /label="MH051"
                     /note="sequence: aaaaacctcccacacctccccc"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     polyA_signal    8242..8292
                     /label="SV40\pA"
                     /ApEinfo_revcolor=#c6c9d1
                     /ApEinfo_fwdcolor=#c6c9d1
     misc_feature    8331..8336
                     /label="inserted NdeI (for color switching)"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     primer          complement(8345..8372)
                     /label="MH008"
                     /note="sequence: ctaaagggaacaaaagctggagctccaccctcgcatttatcagggttattgtctcatgag"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(8416..8434)
                     /label="MH040 binding"
                     /note="sequence: gggaataagggcgacacgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
     primer          complement(8416..8475)
                     /label="MH040"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          complement(8416..8475)
                     /label="MH043"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     misc_feature    8477..9313
                     /label="right_arm"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     primer          complement(9162..9183)
                     /label="MH054"
                     /note="sequence: agagcagggccttagggaagcg"
                     /ApEinfo_revcolor=#c7b0e3
                     /ApEinfo_fwdcolor=#c7b0e3
     CDS             complement(9588..10379)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          10184..10207
                     /label="SL008 Sequencing from Amp rev"
                     /note="sequence: gttgctcttgcccggcgtcaatac"
                     /ApEinfo_revcolor=#d6b295
                     /ApEinfo_fwdcolor=#d6b295
     primer          complement(10187..10207)
                     /label="SL037-Amp fwd"
                     /note="sequence: gtattgacgccgggcaagagc"
                     /ApEinfo_revcolor=#b4abac
                     /ApEinfo_fwdcolor=#b4abac
     CDS             complement(10380..10448)
                     /label="AmpR"
                     /ApEinfo_revcolor=#b7e6d7
                     /ApEinfo_fwdcolor=#b7e6d7
     primer          10412..10431
                     /label="MH040"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#ff9ccd
                     /ApEinfo_fwdcolor=#ff9ccd
     primer          10412..10431
                     /label="MH043"
                     /note="sequence: caataatcaatgtctctagaggccgagacgcttacaatttagggaataagggcgacacgg"
                     /ApEinfo_revcolor=#f8d3a9
                     /ApEinfo_fwdcolor=#f8d3a9
     primer          10413..10431
                     /label="MH040 binding"
                     /note="sequence: gggaataagggcgacacgg"
                     /ApEinfo_revcolor=#84b0dc
                     /ApEinfo_fwdcolor=#84b0dc
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa acgaattcgc cctttgcttt ctctgaccag
     1081 cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct
     1141 acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct ccactccctc
     1201 ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc acttccttct
     1261 cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg tcctctccgg
     1321 gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc ccttttcctt
     1381 ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg acggatgtct
     1441 cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt ctggacaacc
     1501 ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt tctttgcctg
     1561 gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg ggcagctccc
     1621 ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca tggcatcttc
     1681 caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat gtccacttca
     1741 ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc accttatatt
     1801 cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc tccaccccac
     1861 agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag atctggcagc
     1921 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     1981 ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2581 gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcta
     2881 gcaaagcctt aagtaacaaa acttttatga gggacagccc ccccccaaag cccccaggga
     2941 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
     3001 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
     3061 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
     3121 ctggggggat acggggaaaa ggcctccacg gccactagta ttatgcaaca gctgcatata
     3181 gcactgcagt ttactcccta tcagtgatag agagtcaaaa ggccatgccc tccctatcag
     3241 tgatagagag agcagactca aggtggtccc tatcagtgat agagagattg agcaagaggg
     3301 cctccctatc agtgatagag aatagcactg cagtttactc cctatcagtg atagagagtc
     3361 aaaaggccat gccctcccta tcagtgatag agagagcaga ctcaaggtgg tccctatcag
     3421 tgatagagag attgagcaag agggcctccc tatcagtgat agagattgat ccagtcaggg
     3481 cctccctatc agtgatagag aagctatgtg caggtagggc tgtacagtgg gaatgcatga
     3541 gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc
     3601 ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt
     3661 tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg
     3721 caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct
     3781 ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat
     3841 tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg
     3901 agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg
     3961 aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa
     4021 tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca
     4081 agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc
     4141 ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg
     4201 gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc
     4261 gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct
     4321 tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg
     4381 tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc
     4441 cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt ggagtacgtc
     4501 gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga
     4561 gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga
     4621 gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt
     4681 tcaggtgtcg tgagctagcg attacgccaa gctcgaaatt aaccctcact aaagggaaca
     4741 aaagctggag ctccaccgcg gtggcggccg ctctagcccg ggcggatccc ccgggctgca
     4801 ggaattcgat atcaagcttg ccaccatgcc agagccagcg aagtctgctc ccgccccgaa
     4861 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
     4921 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
     4981 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga
     5041 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
     5101 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
     5161 gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatcccc gggtaccggt
     5221 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5281 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     5341 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5401 gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct accccgacca
     5461 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5521 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     5581 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5641 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     5701 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     5761 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     5821 caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     5881 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     5941 caagtaggtc gacctcgagg gggggcccgg taccaggtaa gtgtacccaa ttcgccctat
     6001 agtgagtcgt attacaattc actcgatcgc ccttcccaac agttgcgcag cctgaatggc
     6061 gaatggagat ccaattttta agtgtataat gtgttaaact actgattcta attgtttgtg
     6121 tattttagat tcacagtccc aaggctcatt tcaggcccct cagtcctcac agtctgttca
     6181 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     6241 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     6301 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     6361 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa tctcaaagta
     6421 acaaaacttt tatgagggac agcccccccc caaagccccc agggatgtaa ttacgtccct
     6481 cccccgctag ggggcagcag cgagccgccc ggggctccgc tccggtccgg cgctcccccc
     6541 gcatccccga gccggcagcg tgcggggaca gcccgggcac ggggaaggtg gcacgggatc
     6601 gctttcctct gaacgcttct cgctgctctt tgagcctgca gacacctggg gggatacggg
     6661 gaaaaggcct ccacggccac tagtattatg ccctagggac gtccatatgA ATGTAGTCTT
     6721 ATGCAATACA CTTGTAGTCT TGCAACATGG TAACGATGAG TTAGCAACAT GCCTTACAAG
     6781 GAGAGAAAAA GCACCGTGCA TGCCGATTGG TGGAAGTAAG GTGGTACGAT CGTGCCTTAT
     6841 TAGGAAGGCA ACAGACAGGT CTGACATGGA TTGGACGAAC CACTGAATTG CGCATTGCAG
     6901 AGATAATTGT ATTTAAGTGC CTAGCTCGAT ACAATAAACG CCATTTGACC ATTCACCACA
     6961 TTGGTGTGCA CCCTGCAGat ctagaatgcc agagccagcg aagtctgctc ccgccccgaa
     7021 aaagggctcc aagaaggcgg tgactaaggc gcagaagaaa ggcggcaaga agcgcaagcg
     7081 cagccgcaag gagagctatt ccatctatgt gtacaaggtt ctgaagcagg tccaccctga
     7141 caccggcatt tcgtccaagg ccatgggcat catgaattcg tttgtgaacg acattttcga
     7201 gcgcatcgca ggtgaggctt cccgcctggc gcattacaac aagcgctcga ccatcacctc
     7261 cagggagatc cagacggccg tgcgcctgct gctgcctggg gagttggcca agcacgccgt
     7321 gtccgagggt actaaggcca tcaccaagta caccagcgct aaggatccac cggtcgccac
     7381 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa
     7441 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
     7501 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc
     7561 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca
     7621 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg
     7681 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga
     7741 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt
     7801 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg
     7861 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc
     7921 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt
     7981 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
     8041 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt cttaagcgac
     8101 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
     8161 cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     8221 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     8281 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct catatgtgta
     8341 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     8401 gagtattcaa catttccgtg tcgcccttat tccctaaatt gtaagcgtct cggcctctag
     8461 agacattgat tattgaacta gggacaggat tggtgacaga aaagccccat ccttaggcct
     8521 cctccttcct agtctcctga tattgggtct aacccccacc tcctgttagg cagattcctt
     8581 atctggtgac acacccccat ttcctggagc catctctctc cttgccagaa cctctaaggt
     8641 ttgcttacga tggagccaga gaggatcctg ggagggagag cttggcaggg ggtgggaggg
     8701 aaggggggga tgcgtgacct gcccggttct cagtggccac cctgcgctac cctctcccag
     8761 aacctgagct gctctgacgc ggctgtctgg tgcgtttcac tgatcctggt gctgcagctt
     8821 ccttacactt cccaagagga gaagcagttt ggaaaaacaa aatcagaata agttggtcct
     8881 gagttctaac tttggctctt cacctttcta gtccccaatt tatattgttc ctccgtgcgt
     8941 cagttttacc tgtgagataa ggccagtagc cagccccgtc ctggcagggc tgtggtgagg
     9001 aggggggtgt ccgtgtggaa aactcccttt gtgagaatgg tgcgtcctag gtgttcacca
     9061 ggtcgtggcc gcctctactc cctttctctt tctccatcct tctttcctta aagagtcccc
     9121 agtgctatct gggacatatt cctccgccca gagcagggtc ccgcttccct aaggccctgc
     9181 tctgggcttc tgggtttgag tccttggcaa gcccaggaga ggcgctcagg cttccctgtc
     9241 ccccttcctc gtccaccatc tcatgcccct ggctctcctg ccccttccct acaggggttc
     9301 ctggctctgc tcttcgcctt cttgacgagt tcttctgaat tattaacgct tacaatttcc
     9361 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcatc aggtggcact
     9421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9481 tatccgctca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9541 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     9601 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     9661 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     9721 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     9781 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     9841 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     9901 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     9961 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10021 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10081 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10141 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10201 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10261 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10321 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10381 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10441 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10561 aaaggatctt c
//