LOCUS       Exported                6599 bp ds-DNA     circular UNA 24-NOV-2021
DEFINITION  natural circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 24, 2021 from SnapGene 4.1.6
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="unspecified"
                     /mol_type="genomic DNA"
     promoter        complement(240..344)
                     /label=AmpR promoter
     LTR             359..952
     CDS             1433..1849
                     /label=Truncated gag
     misc_feature    1859..2233
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     regulatory      2257..2266
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2263..2979
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     2483..2503
                     /label=GFP Fw 4
     primer_bind     2518..2536
                     /label=GFP Fw 1
     primer_bind     2567..2588
                     /label=GFP Fw 2
     primer_bind     2615..2634
                     /label=GFP Fw 3
     CDS             2989..3045
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    3046..3079
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     gene            3116..4280
                     /label=CrePI
     primer_bind     complement(3317..3336)
                     /label=MS CRE seq
     intron          3548..3641
                     /label=Protamine intron
     primer_bind     complement(3592..3613)
                     /label=Cre Rv 8
     primer_bind     complement(3633..3656)
                     /label=Cre Rv 7
     primer_bind     complement(3677..3696)
                     /label=Cre Rv 5
     primer_bind     complement(3720..3744)
                     /label=Cre Rv 6
     primer_bind     complement(3801..3821)
                     /label=Cre Rv 1
     primer_bind     complement(3967..3986)
                     /label=Cre Rv 2
     primer_bind     complement(4037..4057)
                     /label=Cre Rv 3
     primer_bind     complement(4129..4148)
                     /label=Cre Rv 4
     CDS             4248..4277
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    4292..4325
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GTATACAT). loxP511 sites are compatible with each
                     other, but incompatible with wild-type loxP sites."
     LTR             4396..4989
     rep_origin      complement(5226..5814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5978..239)
                     /label=Bla
ORIGIN
        1 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
       61 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
      181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
      241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
      301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tcctgcataa
      361 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
      421 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      481 ctgaatatgg gccaaagcgg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      541 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      601 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      661 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      721 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      781 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      841 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      901 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
      961 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     1021 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     1081 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1141 gttcggaaca cccggccgca accctgggag acgtcccagg tcgggggccg tttttgtggc
     1201 ccgacctgag tccaaaaatc ccgatcgttt tggactcttt ggtgcacccc ccttagagga
     1261 gggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
     1321 tttgctttcg gtttgggacc gaagccgcgc cgcgcgtctt gtctgctgca gcatcgttct
     1381 gtgttgtctc tgtctgactg tgtttctgta tttgtctgaa aatatgggcc cgggccagac
     1441 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     1501 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     1561 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     1621 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     1681 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     1741 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     1801 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     1861 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     1921 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     1981 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     2041 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     2101 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     2161 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     2221 atcctctaga ctgccggatc tgattctaga gctagcgcca ccatggtgag caagggcgag
     2281 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     2341 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     2401 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     2461 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     2521 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     2581 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2641 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2701 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2761 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2821 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2881 gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2941 gccgccggga tcactctcgg catggacgag ctgtacaagg gttccggagc cacgaacttc
     3001 tctctgttaa agcaagcagg agacgtggaa gaaaaccccg gtcccataac ttcgtatagt
     3061 atacattata cgaagttatc agaattcggg gggggggggg gggggggggg gctcgagggc
     3121 caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa cgagtgatga
     3181 ggttcgcaag aacctgtgtg actgcttcag ggatcgccag gcgttttctg agcatacctg
     3241 gaaatgtctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga ataaccggaa
     3301 atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg
     3361 tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc
     3421 cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatcagaaa
     3481 agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt
     3541 cgaccaggta agtagagggc tgggctgggc tgtggggggt gtggggtgcg ggacttgggc
     3601 atgtctggga gtccctctca ccacttttct tacctttcta ggttcgttca ctcatggaaa
     3661 atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct tataacaccc
     3721 tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt actgacggtg
     3781 ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca ggtgtagaga
     3841 aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc tctggtgtag
     3901 ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt gccgcgccat
     3961 ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca actcatcgat
     4021 tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct ggacacagtg
     4081 cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg gagatcatgc
     4141 aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac ctggatagtg
     4201 aaacaggggc aatggtgcgc ctgctggaag atggcgatgg accggtggaa caaaaactta
     4261 tttctgaaga agatctgtga tagtaagtcg gataacttcg tatagtatac attatacgaa
     4321 gttatggatc cgcgccacgc ggccgcagtc gacgataaaa taaaagattt tatttagtct
     4381 ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac
     4441 gccattttgg aaggcatgga aaaatacata actgagaata gagaagttca gatcaaggtc
     4501 aggaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     4561 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
     4621 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
     4681 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
     4741 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
     4801 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
     4861 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
     4921 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
     4981 tctttcattc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     5041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     5101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5221 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5401 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5641 tggcctaact acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca
     5701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttgcggcc ggccgcaaat
     5941 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     6001 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     6061 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     6121 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     6181 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     6241 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     6301 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     6361 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     6421 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     6481 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     6541 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacgg
//
