LOCUS       pDB_S_SnapTag_Cr        8777 bp    DNA        circular     23-NOV-2021
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     >pUC57
COMMENT     NcoI, SacI
COMMENT     Sequencing: pQE30 fw (1-1000);  2075rv (2050-1000); 1825 rv
            (3000-2000) ; EGFP C F (3100-4100); pCEPrev (3970-5000); CMV-F
            pCDNA (5100-6100)
COMMENT     
COMMENT     Nco test digest
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1663..1713
                     /locus_tag="HF-Tag"
                     /label="HF-Tag"
                     /ApEinfo_label="HF-Tag"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    804..875
                     /locus_tag="Exon11"
                     /label="Exon11"
                     /ApEinfo_label="Exon11"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3085..3480
                     /locus_tag="BlasticidinR"
                     /label="BlasticidinR"
                     /ApEinfo_label="BlasticidinR"
                     /ApEinfo_fwdcolor="#ff8040"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    950..1050
                     /locus_tag="Exon12"
                     /label="Exon12"
                     /ApEinfo_label="Exon12"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3736..3746
                     /locus_tag="Exon12(1)"
                     /label="Exon12(1)"
                     /ApEinfo_label="Exon12"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3675..3708
                     /locus_tag="LoxP"
                     /label="LoxP"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    978..997
                     /locus_tag="gRNA Cry1-1"
                     /label="gRNA Cry1-1"
                     /ApEinfo_label="gRNA Cry1-1"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4532..4553
                     /locus_tag="Exon13"
                     /label="Exon13"
                     /ApEinfo_label="Exon13"
                     /ApEinfo_fwdcolor="#80ff80"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1821..1868
                     /locus_tag="Synth PolyA"
                     /label="Synth PolyA"
                     /ApEinfo_label="Synth PolyA"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    983..1003
                     /locus_tag="gRNA Cry1-2"
                     /label="gRNA Cry1-2"
                     /ApEinfo_label="gRNA Cry1-2"
                     /ApEinfo_fwdcolor="#3399ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3744..3746
                     /locus_tag="STOP CODON"
                     /label="STOP CODON"
                     /ApEinfo_label="STOP CODON"
                     /ApEinfo_fwdcolor="#ff0000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1896..2238
                     /locus_tag="SV40 ori+pro"
                     /label="SV40 ori+pro"
                     /ApEinfo_label="SV40 ori+pro"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1032..1050
                     /locus_tag="gRNA Cry1-3"
                     /label="gRNA Cry1-3"
                     /ApEinfo_label="gRNA Cry1-3"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3736..3744
                     /locus_tag="gRNA Cry1-3(1)"
                     /label="gRNA Cry1-3(1)"
                     /ApEinfo_label="gRNA Cry1-3"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2038..2234
                     /locus_tag="SV40 early promoter"
                     /label="SV40 early promoter"
                     /ApEinfo_label="SV40 early promoter"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    999..999
                     /locus_tag="mut PAM"
                     /label="mut PAM"
                     /ApEinfo_label="mut PAM"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3013..3078
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_label="P2A"
                     /ApEinfo_fwdcolor="#ff8000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1005..1005
                     /locus_tag="mut Pam"
                     /label="mut Pam"
                     /ApEinfo_label="mut Pam"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1783..1820
                     /locus_tag="LoxP(1)"
                     /label="LoxP(1)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1728..1775
                     /locus_tag="Frt F3"
                     /label="Frt F3"
                     /ApEinfo_label="Frt F3"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1060..1107
                     /locus_tag="FrtSite F"
                     /label="FrtSite F"
                     /ApEinfo_label="FrtSite F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3492..3622
                     /locus_tag="SV40 PolyA signal"
                     /label="SV40 PolyA signal"
                     /ApEinfo_label="SV40 PolyA signal"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    2291..3006
                     /locus_tag="CFP"
                     /label="CFP"
                     /ApEinfo_label="CFP"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4586..4669
                     /locus_tag="hCMV minEnh"
                     /label="hCMV minEnh"
                     /ApEinfo_label="hCMV minEnh"
                     /ApEinfo_fwdcolor="#8080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3076..3078
                     /locus_tag="PuroR"
                     /label="PuroR"
                     /ApEinfo_label="PuroR"
                     /ApEinfo_fwdcolor="#808000"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             1114..1656
                     /codon_start=SNAP-tag(R)
                     /product="human O6-alkylguanine-DNA-alkyltransferase
                     variant that forms covalent bonds with benzylguanine
                     derivatives"
                     /note="fast-reacting variant, optimized for mammalian
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAV
                     EVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKL
                     LKVVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEG
                     GLAVKEWLLAHEGHRLGKPGLG"
                     /locus_tag="SNAP-tag(R)"
                     /label="SNAP-tag(R)"
                     /ApEinfo_label="SNAP-tag(R)"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3627..3674
                     /locus_tag="Frt F3(1)"
                     /label="Frt F3(1)"
                     /ApEinfo_label="Frt F3"
                     /ApEinfo_fwdcolor="#0080c0"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1714..1721
                     /locus_tag="HF-Tag(1)"
                     /label="HF-Tag(1)"
                     /ApEinfo_label="HF-Tag"
                     /ApEinfo_fwdcolor="#ff8080"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6552..6572)
                     /locus_tag="M13-rev"
                     /label="M13-rev"
                     /ApEinfo_label="M13-rev"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     rep_origin      complement(6940..7622)
                     /locus_tag="ColE1 origin"
                     /label="ColE1 origin"
                     /ApEinfo_label="ColE1 origin"
                     /ApEinfo_fwdcolor="gray50"
                     /ApEinfo_revcolor="gray50"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_binding    complement(6578..6600)
                     /locus_tag="LacO"
                     /label="LacO"
                     /ApEinfo_label="LacO"
                     /ApEinfo_fwdcolor="#6495ed"
                     /ApEinfo_revcolor="#6495ed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1074..1107
                     /locus_tag="FRT"
                     /label="FRT"
                     /ApEinfo_label="FRT"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="pink"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     1783..1816
                     /locus_tag="LoxP(2)"
                     /label="LoxP(2)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_recomb     3675..3708
                     /locus_tag="LoxP(3)"
                     /label="LoxP(3)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="steel blue"
                     /ApEinfo_revcolor="orange"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     CDS             complement(7720..8379)
                     /locus_tag="AmpR"
                     /label="AmpR"
                     /ApEinfo_label="AmpR"
                     /ApEinfo_fwdcolor="yellow"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2298..2315)
                     /locus_tag="1927"
                     /label="1927"
                     /ApEinfo_label="1927"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4059..4081)
                     /locus_tag="1010"
                     /label="1010"
                     /ApEinfo_label="1010"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2884..2905
                     /locus_tag="1034"
                     /label="1034"
                     /ApEinfo_label="1034"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     715..735
                     /locus_tag="1000"
                     /label="1000"
                     /ApEinfo_label="1000"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2942..2961
                     /locus_tag="814"
                     /label="814"
                     /ApEinfo_label="814"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2942..2961
                     /locus_tag="838"
                     /label="838"
                     /ApEinfo_label="838"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     942..961
                     /locus_tag="1926"
                     /label="1926"
                     /ApEinfo_label="1926"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     942..963
                     /locus_tag="1823"
                     /label="1823"
                     /ApEinfo_label="1823"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2916..2934
                     /locus_tag="729"
                     /label="729"
                     /ApEinfo_label="729"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2521..2540
                     /locus_tag="734"
                     /label="734"
                     /ApEinfo_label="734"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4256..4278)
                     /locus_tag="1841"
                     /label="1841"
                     /ApEinfo_label="1841"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6556..6572)
                     /locus_tag="43"
                     /label="43"
                     /ApEinfo_label="43"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2060..2077
                     /locus_tag="811"
                     /label="811"
                     /ApEinfo_label="811"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     960..979
                     /locus_tag="1840"
                     /label="1840"
                     /ApEinfo_label="1840"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     637..657
                     /locus_tag="1002"
                     /label="1002"
                     /ApEinfo_label="1002"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2652..2671)
                     /locus_tag="1925"
                     /label="1925"
                     /ApEinfo_label="1925"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2880..2899
                     /locus_tag="1024"
                     /label="1024"
                     /ApEinfo_label="1024"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8756..8774
                     /locus_tag="805"
                     /label="805"
                     /ApEinfo_label="805"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2911..2933
                     /locus_tag="1584"
                     /label="1584"
                     /ApEinfo_label="1584"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2942..2961)
                     /locus_tag="1023"
                     /label="1023"
                     /ApEinfo_label="1023"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4226..4250)
                     /locus_tag="1824"
                     /label="1824"
                     /ApEinfo_label="1824"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     843..862
                     /locus_tag="1054"
                     /label="1054"
                     /ApEinfo_label="1054"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4116..4136)
                     /locus_tag="1013"
                     /label="1013"
                     /ApEinfo_label="1013"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2974..2996
                     /locus_tag="870"
                     /label="870"
                     /ApEinfo_label="870"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2597..2616)
                     /locus_tag="735"
                     /label="735"
                     /ApEinfo_label="735"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     803..822
                     /locus_tag="1260"
                     /label="1260"
                     /ApEinfo_label="1260"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2873..2892)
                     /locus_tag="1825"
                     /label="1825"
                     /ApEinfo_label="1825"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     977..996
                     /locus_tag="1436"
                     /label="1436"
                     /ApEinfo_label="1436"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3580..3600)
                     /locus_tag="792"
                     /label="792"
                     /ApEinfo_label="792"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3748..3775
                     /locus_tag="1967"
                     /label="1967"
                     /ApEinfo_label="1967"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6584..6603)
                     /locus_tag="1069"
                     /label="1069"
                     /ApEinfo_label="1069"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4070..4093)
                     /locus_tag="1012"
                     /label="1012"
                     /ApEinfo_label="1012"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(968..986)
                     /locus_tag="991"
                     /label="991"
                     /ApEinfo_label="991"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     635..654
                     /locus_tag="1004"
                     /label="1004"
                     /ApEinfo_label="1004"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1941..1960)
                     /locus_tag="2052"
                     /label="2052"
                     /ApEinfo_label="2052"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(1897..1917)
                     /locus_tag="2075"
                     /label="2075"
                     /ApEinfo_label="2075"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6568..6591)
                     /locus_tag="30nt_M13R"
                     /label="30nt_M13R"
                     /ApEinfo_label="30nt_M13R"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(4599..4619)
                     /locus_tag="2076"
                     /label="2076"
                     /ApEinfo_label="2076"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2943..2964
                     /locus_tag="EGFP C F"
                     /label="EGFP C F"
                     /ApEinfo_label="EGFP C F"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2335..2356)
                     /locus_tag="EGFP Nrev"
                     /label="EGFP Nrev"
                     /ApEinfo_label="EGFP Nrev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4780..4799
                     /locus_tag="CMVF_pCDNA3"
                     /label="CMVF_pCDNA3"
                     /ApEinfo_label="CMVF_pCDNA3"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6555..6572)
                     /locus_tag="M13R"
                     /label="M13R"
                     /ApEinfo_label="M13R"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(29..51)
                     /locus_tag="PGEX 3'"
                     /label="PGEX 3'"
                     /ApEinfo_label="PGEX 3'"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8691..8708
                     /locus_tag="PQE30 forward"
                     /label="PQE30 forward"
                     /ApEinfo_label="PQE30 forward"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3583..3602
                     /locus_tag="pCEP-Reverse"
                     /label="pCEP-Reverse"
                     /ApEinfo_label="pCEP-Reverse"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3709..3712
                     /locus_tag="LoxP(4)"
                     /label="LoxP(4)"
                     /ApEinfo_label="LoxP"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    6259..6537
                     /locus_tag="PolyA"
                     /label="PolyA"
                     /ApEinfo_label="PolyA"
                     /ApEinfo_fwdcolor="#0080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4670..4872
                     /locus_tag="hCMV promoter"
                     /label="hCMV promoter"
                     /ApEinfo_label="hCMV promoter"
                     /ApEinfo_fwdcolor="#8080ff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3839..3860)
                     /locus_tag="1261"
                     /label="1261"
                     /ApEinfo_label="1261"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3922..3941)
                     /locus_tag="1934"
                     /label="1934"
                     /ApEinfo_label="1934"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4868..4888
                     /locus_tag="pCMV forward"
                     /label="pCMV forward"
                     /ApEinfo_label="pCMV forward"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4867..4886
                     /locus_tag="pCEP-Forward"
                     /label="pCEP-Forward"
                     /ApEinfo_label="pCEP-Forward"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    4967..6241
                     /locus_tag="trunc hCD4"
                     /label="trunc hCD4"
                     /ApEinfo_label="trunc hCD4"
                     /ApEinfo_fwdcolor="#ffa579"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8513..8532
                     /locus_tag="AmpStart"
                     /label="AmpStart"
                     /ApEinfo_label="AmpStart"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(7767..7786)
                     /locus_tag="AmpStop"
                     /label="AmpStop"
                     /ApEinfo_label="AmpStop"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4869..4886
                     /locus_tag="CEP4-for"
                     /label="CEP4-for"
                     /ApEinfo_label="CEP4-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4823..4843
                     /locus_tag="CMV-for"
                     /label="CMV-for"
                     /ApEinfo_label="CMV-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3583..3602
                     /locus_tag="EBV-rev"
                     /label="EBV-rev"
                     /ApEinfo_label="EBV-rev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2916..2934
                     /locus_tag="EGFP-C-F-31"
                     /label="EGFP-C-F-31"
                     /ApEinfo_label="EGFP-C-F-31"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2947..2964
                     /locus_tag="EGFP-C-for"
                     /label="EGFP-C-for"
                     /ApEinfo_label="EGFP-C-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(3492..3509)
                     /locus_tag="EGFP-C-Rev"
                     /label="EGFP-C-Rev"
                     /ApEinfo_label="EGFP-C-Rev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2925..2941
                     /locus_tag="EGFP-for"
                     /label="EGFP-for"
                     /ApEinfo_label="EGFP-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4780..4799
                     /locus_tag="EGFP-N-for"
                     /label="EGFP-N-for"
                     /ApEinfo_label="EGFP-N-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2365..2383)
                     /locus_tag="EGFP-N-R+33"
                     /label="EGFP-N-R+33"
                     /ApEinfo_label="EGFP-N-R+33"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2398..2416)
                     /locus_tag="EGFP-N-rev"
                     /label="EGFP-N-rev"
                     /ApEinfo_label="EGFP-N-rev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(57..76)
                     /locus_tag="GEX3-rev"
                     /label="GEX3-rev"
                     /ApEinfo_label="GEX3-rev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(29..47)
                     /locus_tag="GEX3-rev-25"
                     /label="GEX3-rev-25"
                     /ApEinfo_label="GEX3-rev-25"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4836..4852
                     /locus_tag="IRES-for"
                     /label="IRES-for"
                     /ApEinfo_label="IRES-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6570..6589)
                     /locus_tag="LacOp-for"
                     /label="LacOp-for"
                     /ApEinfo_label="LacOp-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6556..6572)
                     /locus_tag="M13r"
                     /label="M13r"
                     /ApEinfo_label="M13r"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     3529..3550
                     /locus_tag="pCI-rev"
                     /label="pCI-rev"
                     /ApEinfo_label="pCI-rev"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(2332..2348)
                     /locus_tag="PEN1-737R"
                     /label="PEN1-737R"
                     /ApEinfo_label="PEN1-737R"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4862..4881
                     /locus_tag="pTRE-for"
                     /label="pTRE-for"
                     /ApEinfo_label="pTRE-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     complement(6604..6623)
                     /locus_tag="pUCM13-rev-157"
                     /label="pUCM13-rev-157"
                     /ApEinfo_label="pUCM13-rev-157"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     8757..8775
                     /locus_tag="QE-for"
                     /label="QE-for"
                     /ApEinfo_label="QE-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     2069..2088
                     /locus_tag="SV40-for"
                     /label="SV40-for"
                     /ApEinfo_label="SV40-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     primer_bind     4923..4939
                     /locus_tag="TET-CMV-for"
                     /label="TET-CMV-for"
                     /ApEinfo_label="TET-CMV-for"
                     /ApEinfo_fwdcolor="orchid"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatggg ccggccctta agGTTTAAAC tcatattttt ccataaactg gacacactga
      241 cagaaatgtt tccctaaaac atacttctct gttctagcca acctattctc ctataatagt
      301 gtttgttttt agaagggttg ataggattgc ttaggctagg taactgccac atagtctgtg
      361 tcttctgctt gtcgtatgat ttgaattgtt taactcatcg ggcaggccct tattccctta
      421 tcctcaagga aatatgtttg gaggagctct gaaggaaaag cgtgggaaaa gggacaagag
      481 tgagggagga atgtctatct cactcttgcc cctatgtttt accttcatgg tcaagtttca
      541 aattgtcatc tagtaaggaa atgtttttta gaaaatgatc atcaatgatt tttcctttga
      601 gctgtcaaca cttctgtgag cctaaattta agttttcctg gtttgagcat gtgatttttg
      661 gttcatagga agattgagat ttagtcttaa aagcatataa atgaccttaa gtacaagcgc
      721 taaaagttgg atttgtagac ttaataatac atacatcttg gatttgattt taagtaattt
      781 tactgtgttt taaatactaa cagGTTGCTC TCAAGGGAGT GGTATTTTAC ACTATGCTCA
      841 TGGCGACAGT CAGCAAACTC ACCTGTTGAA GCAAGgtaag aatgaagcat tggagcatac
      901 tgttcttttt ccttttccta tcttaaacat acatttttta aatgtgcagG AAGAAGCTCC
      961 ATGGGCACTG GTCTCAGTGG TGGGAAACGT CCTAGTCAaG AAGAaGACAC ACAGAGTATT
     1021 GGTCCTAAAG TCCAGAGACA GAGCACTAAT agcggccgcG AAGTTCCTAT TCcGAAGTTC
     1081 CTATTCtcta gaaaGtATAG GAACTTCacc ggtgacaaag actgcgaaat gaagcgcacc
     1141 accctggata gccctctggg caagctggaa ctgtctgggt gcgaacaggg cctgcaccgt
     1201 atcatcttcc tgggcaaagg aacatctgcc gccgacgccg tggaagtgcc tgccccagcc
     1261 gccgtgctgg gcggaccaga gccactgatg caggccaccg cctggctcaa cgcctacttt
     1321 caccagcctg aggccatcga ggagttccct gtgccagccc tgcaccaccc agtgttccag
     1381 caggagagct ttacccgcca ggtgctgtgg aaactgctga aagtggtgaa gttcggagag
     1441 gtcatcagct acagccacct ggccgccctg gccggcaatc ccgccgccac cgccgccgtg
     1501 aaaaccgccc tgagcggaaa tcccgtgccc attctgatcc cctgccaccg ggtggtgcag
     1561 ggcgacctgg acgtgggggg ctacgagggc gggctcgccg tgaaagagtg gctgctggcc
     1621 cacgagggcc acagactggg caagcctggg ctgggtacta gtCACCATCA CCATCACCAT
     1681 GGTAGCGGTG ACTACAAAGA CGATGACGAC AAGgctagca aAGATCTGAA GTTCCTATTC
     1741 cGAAGTTCCT ATTCttcaaa taGtATAGGA ACTTCAGATC TaATAACTTC GTATAGCATA
     1801 CATTATACGA AGTTATTttg AATAAAATAT CTTTATTTTC ATTACATCTG TGTGTTGGTT
     1861 TTTTGTGTTG AGCTGATTTA ACAAAAATTT AACGCTGTGG AATGTGTGTC AGTTAGGGTG
     1921 TGGAAAGTCC CCAGGCTCCC CAGGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT
     1981 CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC
     2041 ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC
     2101 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG
     2161 CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC
     2221 TAGGCTTTTG CAAAAAGCTA TTGTATATCC ATTTTCGGAT CTGATCAGca cTGAGCAggt
     2281 accgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     2341 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     2401 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     2461 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     2521 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     2581 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     2641 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     2701 ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag
     2761 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     2821 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     2881 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     2941 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     3001 tacaaggaat tcggaagcgg agctactaac ttcagcctgc tgaagcaggc tggagacgtg
     3061 gaggagaacc ctggacctca cgtgGCCAAG CCTTTGTCTC AAGAAGAATC CACCCTCATT
     3121 GAAAGAGCAA CGGCTACAAT CAACAGCATC CCCATCTCTG AAGACTACAG CGTCGCCAGC
     3181 GCAGCTCTCT CTAGCGACGG CCGCATCTTC ACTGGTGTCA ATGTATATCA TTTTACTGGG
     3241 GGACCTTGTG CAGAACTCGT GGTGCTGGGC ACTGCTGCTG CTGCGGCAGC TGGCAACCTG
     3301 ACTTGTATCG TCGCGATCGG AAATGAGAAC AGGGGCATCT TGAGCCCCTG CGGACGGTGC
     3361 CGACAGGTGC TTCTCGATCT GCATCCTGGG ATCAAAGCCA TAGTGAAGGA CAGTGATGGA
     3421 CAGCCGACGG CAGTTGGGAT TCGTGAATTG CTGCCCTCTG GTTATGTGTG GGAGGGCTAA
     3481 ctcgagACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
     3541 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
     3601 TCAATGTATC TTATCATGTC TGTATAGAAG TTCCTATTCc GAAGTTCCTA TTCttcaaat
     3661 aGtATAGGAA CTTCATAACT TCGTATAGCA TACATTATAC GAAGTTATtt gaTTAATTAA
     3721 GCGATCGCTT CGAAtGCACT AATTAGgtaa atattttaga gctgtatttc ttgctttaga
     3781 agagtatata attaacataa attaagataa tttcaaaaat ggagcaaatc tctattttca
     3841 aaccagaaaa tcttgaggca ttaattttta agcaattttt acaaactcag ttaatttttg
     3901 gtcaagagac atgcatctgt actggagaaa ttgttgcacc agttttatat tcatctgaac
     3961 caatgctctt taaattagag atgtttatga ttttgtggtc aagttttttc ttagaaaaag
     4021 acaacttttt tatttcctta ctatgtaact atgagtctaa aacaattaaa gtggcctgtt
     4081 tattttagtg acattaatat aatcttttta tgaactttcc cctaaatctt tgcctcttaa
     4141 atgttgataa atttctttta tctgttatga tgcttctaaa atctaaatta tttccaattt
     4201 gggaatagtt caaaattttt taaaatgctg gcccttatta gaagtatcag aaagccttgc
     4261 ctgcattcaa tttaattgga tttgggatgt cattttgtga tttaaattaa tatgaaaaat
     4321 atttatacgt tggatttgcc agtttttaaa aattttctgt ttcttcagtt tctaacactg
     4381 tttacatttt ttattgctta gtttttttat gtcaacctaa ttagactata agtatcttga
     4441 agataaggtc aataaacact catcacattt ttgtcattga attatttgca atcaagcttt
     4501 acctagtttt tttttccccc ttaaatcaca gAAAACATTC AGGAGGAATA CTGGTCGACa
     4561 agcttGCATG CCTGCAGGTC AATTCCCTGG CATTATGCCC AGTACATGAC CTTATGGGAC
     4621 TTTCCTACTT GGCAGTACAT CTACGTATTA GTCATCGCTA TTACCATGGT GATGCGGTTT
     4681 TGGCAGTACA TCAATGGGCG TGGATAGCGG TTTGACTCAC GGGGATTTCC AAGTCTCCAC
     4741 CCCATTGACG TCAATGGGAG TTTGTTTTGG CACCAAAATC AACGGGACTT TCCAAAATGT
     4801 CGTAACAACT CCGCCCCATT GACGCAAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
     4861 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG ATCGCCTGGA GACGCCATCC ACGCTGTTTT
     4921 GACCTCCATA GAAGACACCG GGACCGATCC AGCCTGGGGA TCCACCATGA ACCGGGGAGT
     4981 CCCTTTTAGG CACTTGCTTC TGGTGCTGCA ACTGGCGCTC CTCCCAGCAG CCACTCAGGG
     5041 AAAGAAAGTG GTGCTGGGCA AAAAAGGGGA TACAGTGGAA CTGACCTGTA CAGCTTCCCA
     5101 GAAGAAGAGC ATACAATTCC ACTGGAAAAA CTCCAACCAG ATAAAGATTC TGGGAAATCA
     5161 GGGCTCCTTC TTAACTAAAG GTCCATCCAA GCTGAATGAT CGCGCTGACT CAAGAAGAAG
     5221 CCTTTGGGAC CAAGGAAACT TCCCCCTGAT CATCAAGAAT CTTAAGATAG AAGACTCAGA
     5281 TACTTACATC TGTGAAGTGG AGGACCAGAA GGAGGAGGTG CAATTGCTAG TGTTCGGATT
     5341 GACTGCCAAC TCTGACACCC ACCTGCTTCA GGGGCAGAGC CTGACCCTGA CCTTGGAGAG
     5401 CCCCCCTGGT AGTAGCCCCT CAGTGCAATG TAGGAGTCCA AGGGGTAAAA ACATACAGGG
     5461 GGGGAAGACC CTCTCCGTGT CTCAGCTGGA GCTCCAGGAT AGTGGCACCT GGACATGCAC
     5521 TGTCTTGCAG AACCAGAAGA AGGTGGAGTT CAAAATAGAC ATCGTGGTGC TAGCTTTCCA
     5581 GAAGGCCTCC AGCATAGTCT ATAAGAAAGA GGGGGAACAG GTGGAGTTCT CCTTCCCACT
     5641 CGCCTTTACA GTTGAAAAGC TGACGGGCAG TGGCGAGCTG TGGTGGCAGG CGGAGAGGGC
     5701 TTCCTCCTCC AAGTCTTGGA TCACCTTTGA CCTGAAGAAC AAGGAAGTGT CTGTAAAACG
     5761 GGTTACCCAG GACCCTAAGC TCCAGATGGG CAAGAAGCTC CCGCTCCACC TCACCCTGCC
     5821 CCAGGCCTTG CCTCAGTATG CTGGCTCTGG AAACCTCACC CTGGCCCTTG AAGCGAAAAC
     5881 AGGAAAGTTG CATCAGGAAG TGAACCTGGT GGTGATGAGA GCCACTCAGC TCCAGAAAAA
     5941 TTTGACCTGT GAGGTGTGGG GACCCACCTC CCCTAAGCTG ATGCTGAGCT TGAAACTGGA
     6001 GAACAAGGAG GCAAAGGTCT CGAAGCGGGA GAAGGCGGTG TGGGTGCTGA ACCCTGAGGC
     6061 GGGGATGTGG CAGTGTCTGC TGAGTGACTC GGGACAGGTC CTGCTGGAAT CCAACATCAA
     6121 GGTTCTGCCC ACATGGTCGA CCCCGGTGCA GCCAATGGCC CTGATTGTGC TGGGGGGCGT
     6181 CGCCGGCCTC CTGCTTTTCA TTGGGCTAGG CATCTTCTTC TGTGTCAGGT GCCGGCACTG
     6241 AAGGCGACCT GCGGATCCCA CGTCACTATT GTATACTCTA TATTATACTC TATGTTATAC
     6301 TCTGTAATCC TACTCAATAA ACGTGTCACG CCTGTGAAAC CGTACTAAGT CTCCCGTGTC
     6361 TTCTTATCAC CATCAGGTGA CATCCTCGCC CAGGCTGTCA ATCATGCCGG TATCGATTCC
     6421 AGTAGCACCG GCCCCACGCT GACAACCCAC TCTTGCAGCG TTAGCAGCGC CCCTCTTAAC
     6481 AAGCCGACCC CCACCAGCGT CGCGGTTACT AACACTCCTC TCCCCGACCT GCAGCCCaag
     6541 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     6601 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     6661 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     6721 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6781 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     6841 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     6901 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     6961 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7021 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7081 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7141 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7201 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7261 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7321 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7381 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7441 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7501 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7561 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     7621 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     7681 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     7741 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     7801 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     7861 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     7921 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     7981 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     8041 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     8101 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     8161 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     8221 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     8281 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     8341 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     8401 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     8461 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     8521 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     8581 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     8641 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     8701 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     8761 cacgaggccc tttcgtc    
//
