LOCUS       pJFRC7-JEDI-2P          9436 bp    DNA     circular UNK 30-NOV-2021
DEFINITION  .
ACCESSION   <unknown (modified)
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(3308..3328)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="38.59649122807017"
                     /fragment="True"
                     /other="misc feature"
                     /label="MCS"
     protein_bind    3328..3421
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="5X UAS"
     protein_bind    3456..3549
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="5X UAS"
     protein_bind    3584..3677
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="5X UAS"
     protein_bind    3712..3805
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="5X UAS"
     promoter        3831..4068
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="hsp70 promoter"
     intron          4084..4149
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
                     /label="Mhc intron"
     CDS             4171..5457
                     /created_by="User"
                     /label="JEDI-2P"
     CDS             4624..5342
                     /note="pLannotate"
                     /database="fpbase"
                     /identity="90.1"
                     /match_length="97.95918367346938"
                     /fragment="False"
                     /other="CDS"
                     /label="cEGFP"
     CDS             5465..5538
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="14.450867052023122"
                     /fragment="True"
                     /other="CDS"
                     /label="small t antigen"
     intron          5541..5605
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="intron"
                     /label="small t intron"
     CDS             5605..5897
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="13.841807909604519"
                     /fragment="True"
                     /other="CDS"
                     /label="large T antigen"
     CDS             5922..6004
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="3.954802259887006"
                     /fragment="True"
                     /other="CDS"
                     /label="large T antigen"
     polyA_signal    complement(6028..6161)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.265"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA signal"
                     /label="SV40 poly(A) signal"
     promoter        6289..6392
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     CDS             6394..7253
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
     rep_origin      7425..8012
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     protein_bind    complement(8100..8168)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="attB"
ORIGIN      
        1 ttcagggagc ggccaactag ccgagaacct cacctatgcc tggcacaata tggacatctt
       61 tggggcggtc aatcagccgg gctccggatg gcggcagctg gtcaaccgga cacgcggact
      121 attctgcaac gagcgacaca taccggcgcc caggaaacat ttgctcaaga acggtgagtt
      181 tctattcgca gtcggctgat ctgtgtgaaa tcttaataaa gggtccaatt accaatttga
      241 aactcagttt gcggcgtggc ctatccgggc gaacttttgg ccgtgatggg cagttccggt
      301 gccggaaaga cgaccctgct gaatgccctt gcctttcgat cgccgcaggg catccaagta
      361 tcgccatccg ggatgcgact gctcaatggc caacctgtgg acgccaagga gatgcaggcc
      421 aggtgcgcct atgtccagca ggatgacctc tttatcggct ccctaacggc cagggaacac
      481 ctgattttcc aagccatggt gcggatgcca cgacatctga cctatcggca gcgagtggcc
      541 cgcgtggatc aggtgatcca ggagctttcg ctcagcaaat gtcagcacac gatcatcggt
      601 gtgcccggca gggtgaaagg tctgtccggc ggagaaagga agcgtctggc attcgcctcc
      661 gaggctctaa ccgatccgcc gcttctgatc tgcgatgagc ccacctccgg actggactcc
      721 tttaccgccc acagcgtcgt ccaggtgctg aagaagctgt cgcagaaggg caagaccgtc
      781 atcctgacca ttcatcagcc gtcttccgag ctgtttgagc tctttgacaa gatccttctg
      841 atggccgagg gcagggtagc tttcttgggc actcccagcg aagccgtcga cttcttttcc
      901 tagtgagttc gatgtgttta ttaagggtat ctagtattac ataacatctc aactcctatc
      961 cagcgtgggt gcccagtgtc ctaccaacta caatccggcg gacttttacg tacaggtgtt
     1021 ggccgttgtg cccggacggg agatcgagtc ccgtgatcgg atcgccaaga tatgcgacaa
     1081 ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca aaaatctgga
     1141 gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt tcatgcagtt
     1201 ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc tcgtaaaagt
     1261 gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata acgcttacaa
     1321 ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca cccttcttag
     1381 tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat ttcatttaac
     1441 tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt
     1501 ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat caacggagcc
     1561 atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt
     1621 catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa tcgattcgat
     1681 ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg
     1741 ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc tcacagtgcc
     1801 actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt
     1861 cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct
     1921 aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat
     1981 accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa
     2041 atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg
     2101 ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc
     2161 gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt
     2221 gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg
     2281 ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt tttttttacc
     2341 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg
     2401 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca
     2461 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc
     2521 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca
     2581 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg
     2641 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact
     2701 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca
     2761 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat
     2821 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt
     2881 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt
     2941 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca
     3001 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg
     3061 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact
     3121 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca
     3181 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     3241 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca
     3301 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct
     3361 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg
     3421 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt
     3481 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact
     3541 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg
     3601 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc
     3661 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact
     3721 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc
     3781 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata
     3841 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc
     3901 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta
     3961 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac
     4021 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa
     4081 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc
     4141 tttacttcag gcggccgcgg ctcgagcaaa atggagacga ctgtgaggta tgaacagggg
     4201 tcagagctca ctaaaacttc gagctctcca acagcagatg agcccacgat aaagattgat
     4261 gatggtcgtg atgagggtaa tgaacaagac agctgttcca ataccattag gagaaaaatt
     4321 tccccgtttg tgatgtcatt tggattcaga gtatttggag ttgtgcttat cattgtagac
     4381 atcatagtgg tgattgtgga tctggccatc agtgagaaga aaagaggcat tagagagatt
     4441 cttgaaggtg tttccctggc tatagcactc ttcttccttg ttgatgttct catgagagtg
     4501 tttgttgaag gcttcaagaa ctatttccgg tccaaactgg ttactttgga tgcagtcata
     4561 gtagtgggca ctctgctaat taatatgacc tactccttct ctgaccttgc tgcctttaac
     4621 agcgagaacg tgtatattac cgcggataaa cagaaaaacg gcattaaagc gaactttacc
     4681 gtgcgccata acgtggaaga tggcagcgtg cagctggcgg atcattatca gcagaacacc
     4741 ccgattggcg atggcccggt gctgctgccg gataaccatt atctgagcca ccagaccgtg
     4801 ctgagcaaag atccgaacga aaaacgcgat cacatggtgc tgctggaatt tgtgaccgca
     4861 gcgggcatta cacacggcat ggatgaactg tatggcggca ccggcggcag cgcgagccag
     4921 ggcgaagaac tgtttaccgg cgtggtgccg attctggtgg aactggatgg cgatgtgaac
     4981 ggccataaat ttagcgtgcg cggcgaaggc gaaggcgatg cgaccattgg caaactgacc
     5041 ctgaaattta tttgcaccac cggcaaacta ccggtgccgt ggccgaccct ggtgaccacc
     5101 ttaacctatg gcgtgcagtg ctttagccgc tatccggatc atatgaaacg ccatgatttt
     5161 tttaaaagcg cgatgccgga aggctatgtg caggaacgca ccattagctt taaagatgat
     5221 ggcaaatata aaacccgcgc ggtggtgaaa tttgaaggcg ataccctggt gaaccgcatt
     5281 gaactgaaag gcaccgattt taaagaagat ggcaacattc tggggcataa actggaatat
     5341 gatacagatc agatgccgca catggttact cttcttcgag ttctgaaaat tgttatctta
     5401 ataagaatat ttcgcctggc ttcacagaag aaacaacttg aagtggtaac ctaatgatct
     5461 agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     5521 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     5581 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     5641 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     5701 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     5761 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     5821 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     5881 aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     5941 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     6001 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     6061 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6121 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcgatctggc cggccgttta
     6181 aacgaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     6241 gataataatg gtttcttaga ctcaggtggc acttttcggg gaaatgtgcg cggaacccct
     6301 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     6361 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     6421 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     6481 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     6541 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     6601 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6661 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6721 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6781 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6901 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6961 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7021 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7081 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     7141 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     7201 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     7261 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     7321 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     7381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7561 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8041 ttcctggcct tttgctggcc ttttgctcac atgttaccgt cgacgatgta ggtcacggtc
     8101 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
     8161 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
     8221 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
     8281 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
     8341 acggtcacgg cgggcatgtc gacaagccga acatatgggc gcgcctagta tgtatgtaag
     8401 ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt ttactgcact
     8461 ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg
     8521 taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt
     8581 ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct
     8641 ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt gcattttgtc
     8701 agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca aagtgctgtg
     8761 ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg tggtgattgg
     8821 ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca agaggatcag
     8881 gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa caatgtgagt
     8941 agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt gcaacatcaa
     9001 attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatt gcacaaacaa
     9061 atagtgacac gaaacagatt attctggtag ctgttctcgc tatataagac aatttttgag
     9121 atcatatcat gatcaagaca tctaaaggca ttcattttcg actatattct tttttacaaa
     9181 aaatataaca accagatatt ttaagctgat cctagatgca caaaaaataa ataaaagtat
     9241 aaacctactt cgtaggatac ttcggggtac tttttgttcg gggttagatg agcataacgc
     9301 ttgtagttga tatttgagat cccctatcat tgcagggtga cagcggagcg gcttcgcaga
     9361 gctgcattaa ccagggcttc gggcaggcca aaaactacgg cacgctccgg ccacccagtc
     9421 cgccggagga ctccgg
//
